Mouse Adiponectin ELISA Kit

Mouse Adiponectin ELISA Kit (ab226900) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Adiponectin protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Adiponectin with 26 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Adiponectin is a 30kDa monomer that combines to form trimers (low molecular weight), hexamers (middle molecular weight), and other higher molecular weight multimers. Adiponectin is an important adipokine involved in fat metabolism and insulin sensitivity. Insulin stimulates the secretion of adiponectin. Decreased levels of adiponectin are associated with insulin resistance, whereas high levels of adiponectin are associated with reduced risk of type-2 diabetes.