Skip to main content

Mouse ALT ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse ALT in Heparin Plasma, Citrate plasma, Serum, EDTA Plasma samples.

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Mouse

Range

46.88 - 3000 pg/mL

Assay time

1h 30m

Sensitivity

= 4 pg/mL

Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Shipping To:
Korea, Rep of

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Images

Target data

What's included?

1 x 384 Tests
Components
10X Mouse ALT Capture Antibody
1 x 600 µL
10X Mouse ALT Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
384 well CaptSure™ microplates
1 x 1 Unit
5X Cell Extraction Buffer PTR (ab193970)
1 x 50 mL
Antibody Diluent 5BR
1 x 6 mL
Mouse ALT Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Stop Solution
2 x 12 mL
TMB Development Solution
2 x 12 mL

Recommended products

  1. Loading...
  2. Loading...
  3. Loading...
  4. Loading...

Mouse ALT ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse ALT in Heparin Plasma, Citrate plasma, Serum, EDTA Plasma samples.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Mouse

Range

46.88 - 3000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 4 pg/mL

Precision

Intra assay

Sample
Serum
n
8
mean
-
SD
-
C.V.
6.4

Inter assay

Sample
Serum
n
3
mean
-
SD
-
C.V.
7

Recovery

Sample specific recovery

Sample type
Serum
Average %
= 95
Range
80 - 113 %
Sample type
EDTA Plasma
Average %
= 108
Range
103 - 114 %
Sample type
Heparin Plasma
Average %
= 91
Range
85 - 95 %
Sample type
Citrate plasma
Average %
= 95
Range
86 - 113 %

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Mouse ALT ELISA kit (ab282882) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse ALT protein in mouse serum and plasma samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse ALT with 4 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

ALT is an aminotransferase that plays a key role in metabolism. It forms pyruvate through the reversible transamination of alanine and 2-oxoglutarate; this reaction is pivotal for gluconeogenesis and amino acid degradation. It is part of a key blood panel diagnostic that is indicative of liver health.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

There was a problem

We can’t download that datasheet. Please try again. If you need help, contact our Customer Services team at technical@abcam.com