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Mouse ALT ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse ALT in Heparin Plasma, Citrate plasma, Serum, EDTA Plasma samples.
Alternative names=Alanine aminotransferase 1, ALT1, Glutamate pyruvate transaminase 1, Glutamic--alanine transaminase 1, Glutamic--pyruvic transaminase 1, GPT 1, Gpt, Gpt1
Colorimetric
Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
Sandwich (quantitative)
Mouse
46.88 - 3000 pg/mL
1h 30m
= 4 pg/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Mouse ALT ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse ALT in Heparin Plasma, Citrate plasma, Serum, EDTA Plasma samples.
Alternative names=Alanine aminotransferase 1, ALT1, Glutamate pyruvate transaminase 1, Glutamic--alanine transaminase 1, Glutamic--pyruvic transaminase 1, GPT 1, Gpt, Gpt1
Colorimetric
Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
Sandwich (quantitative)
Mouse
46.88 - 3000 pg/mL
1h 30m
Pre-coated microplate (12 x 8 well strips)
= 4 pg/mL
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 6.4 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 7 |
Sample type | Average % | Range |
---|---|---|
Sample type Serum | Average % = 95 | Range 80 - 113 % |
Sample type EDTA Plasma | Average % = 108 | Range 103 - 114 % |
Sample type Heparin Plasma | Average % = 91 | Range 85 - 95 % |
Sample type Citrate plasma | Average % = 95 | Range 86 - 113 % |
Blue Ice
+4°C
+4°C
Mouse ALT ELISA kit (ab282882) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse ALT protein in mouse serum and plasma samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse ALT with 4 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
ALT is an aminotransferase that plays a key role in metabolism. It forms pyruvate through the reversible transamination of alanine and 2-oxoglutarate; this reaction is pivotal for gluconeogenesis and amino acid degradation. It is part of a key blood panel diagnostic that is indicative of liver health.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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