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Mouse IL-6 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse IL-6 in Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum samples.
Alternative names=Interleukin-6, IL-6, B-cell hybridoma growth factor, Interleukin HP-1, Il6, Il-6
- Detection method
Colorimetric
- Sample types
Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Mouse
- Range
15.6 - 1000 pg/mL
- Assay time
1h 30m
- Sensitivity
= 11.3 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Mouse IL-6 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse IL-6 in Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum samples.
Alternative names=Interleukin-6, IL-6, B-cell hybridoma growth factor, Interleukin HP-1, Il6, Il-6
Key facts
- Detection method
Colorimetric
- Sample types
Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Mouse
- Range
15.6 - 1000 pg/mL
- Assay time
1h 30m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 11.3 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Spleen CCS | n 5 | mean - | SD - | C.V. 3.9 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Spleen CCS | n 3 | mean - | SD - | C.V. 3.4 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 107 | Range 103 - 114 % |
Sample type EDTA Plasma | Average % = 112 | Range 109 - 116 % |
Sample type Heparin Plasma | Average % = 98 | Range 95 - 103 % |
Sample type Citrate plasma | Average % = 99 | Range 96 - 102 % |
Sample type Cell culture media | Average % = 100 | Range 89 - 112 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Mouse IL-6 ELISA Kit (ab222503) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-6 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-6 with 11.3 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
ASSAY SPECIFICITY
This kit recognizes both native and recombinant mouse IL-6 protein in serum, plasma, and cell culture supernatant samples only. Cell and tissue extract samples have not been tested with this kit.
SPECIES REACTIVITY
This kit recognizes mouse and rat IL-6 protein.
Rat IL-6 protein was measured at 250pg/mL and 100% cross reactivity was observed. Human IL-6 protein was measured at 1000pg/mL no cross reactivity was observed.
Interleukin 6 (IL-6) is a cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response and plays an essential role in the final differentiation of B-cells into Ig-secreting cells. IL-6 is involved in lymphocyte and monocyte differentiation and IL-6 induces myeloma and plasmacytoma growth as well as nerve cells differentiation. B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS are all responsive to IL-6. IL-6 is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance
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