Mouse IP-10 ELISA Kit (CXCL10)

Mouse IP-10 ELISA Kit (CXCL10) (ab260067) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IP-10 (CXCL10) protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IP-10 (CXCL10) with 21.75 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

ASSAY SPECIFICITY: This kit recognizes both native and recombinant mouse IP-10 protein in serum, citrate plasma, and cell culture supernatant samples only.

Cell and tissue extract samples have not been tested with this kit.

This kit is incompatible with heparin plasma and EDTA plasma samples.

CROSS REACTIVITY: Recombinant human IP-10 and rat IP-10 were each prepared at 50 ng/mL and 1.5 ng/mL and assayed for cross reactivity. No cross reactivity was observed.

SPECIES REACTIVITY: This kit recognizes mouse IP-10 protein.

No signal was observed in 50% serum samples from the following species: Human, Rat, Cow.

C-X-C motif chemokine 10 (CXCL10 or IP-10) is a small 10.8kD protein that is secreted by several cell types in response to interferon-gamma (IFNg). These cell types include monocytes, endothelial cells and fibroblasts. Upon secretion, CXCL10 is cleaved into an 8.7kD biologically active protein to function in chemotaxis for T-cells, NK cells, monocytes/macrophages and dendritic cells. In addition, CXCL10 has antitumor activity through the inhibition of bone marrow colony formation and angiogenesis. CXCL10 elicits its effects by binding to the cell surface chemokine receptor 3 (CXCR3).