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Mouse NT-ProANP ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse NT-ProANP in Cell culture media, Heparin Plasma, Cell culture supernatant, EDTA Plasma samples.
Alternative names=Natriuretic peptides A, Atrial natriuretic factor prohormone, Atrial natriuretic peptide prohormone, Atriopeptigen, Cardiodilatin, preproCDD-ANF, preproANF, proANF, preproANP, proANP, CDD, Pnd, Nppa
- Detection method
Colorimetric
- Sample types
Cell culture media, Heparin Plasma, Cell culture supernatant, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Mouse
- Range
125 - 8000 pg/mL
- Assay time
1h 30m
- Sensitivity
= 43.4 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Mouse NT-ProANP ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse NT-ProANP in Cell culture media, Heparin Plasma, Cell culture supernatant, EDTA Plasma samples.
Alternative names=Natriuretic peptides A, Atrial natriuretic factor prohormone, Atrial natriuretic peptide prohormone, Atriopeptigen, Cardiodilatin, preproCDD-ANF, preproANF, proANF, preproANP, proANP, CDD, Pnd, Nppa
Key facts
- Detection method
Colorimetric
- Sample types
Cell culture media, Heparin Plasma, Cell culture supernatant, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Mouse
- Range
125 - 8000 pg/mL
- Assay time
1h 30m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 43.4 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Plasma | n 8 | mean - | SD - | C.V. 3.6 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Plasma | n 3 | mean - | SD - | C.V. 4.9 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 99 | Range 84 - 111 % |
Sample type EDTA Plasma | Average % = 88 | Range 80 - 97 % |
Sample type Cell culture media | Average % = 110 | Range 99 - 122 % |
Sample type Heparin Plasma | Average % = 92 | Range 81 - 104 % |
Sample type Citrate plasma | Average % = 100 | Range 85 - 117 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Mouse NT-ProANP ELISA Kit (ab267800) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of NT-ProANP protein in cell culture supernatant, edta plasma, hep plasma, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse NT-ProANP with 43.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Mouse NT-ProANP is the propeptide portion of the 129 amino acids long Atrial Natriuretic Peptide (ANP) preproprotein. ANP is primarily synthesized in the heart and is secreted in response to atrial distention. The ANP proprotein is cleaved by serine protease corin to produce the functional hormone, atrial natriuretic factor (ANF). ANF circulates in plasma; it is involved in cardiovascular homeostasis through the regulation of natriuresis, diuresis, and vasodilation directed toward reduction of body fluid and blood pressure regulation. NT-ProANP circulates at low levels at the early stages of hypertension and increases during chronic heart failure. Mouse NT-ProANP is 94% identical to rat NT-ProANP on amino acid level. This kit recognizes mouse NT-ProANP protein (amino acids 25 – 122 of Uniprot # P05125). This kit does not recognize mouse ANP proprotein (amino acids 25 – 152 of Uniprot # P05125).
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