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Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse/Rat ACTH (adrenocorticotropic hormone) in Heparin Plasma, Citrate plasma, Serum, EDTA Plasma samples.
Alternative names=Adrenocorticotropic hormone, Adrenocorticotropin, Alpha melanocyte stimulating hormone, Beta LPH, Beta melanocyte stimulating hormone, Beta-endorphin, Corticotropin lipotropin, Corticotropin-like intermediary peptide, Gamma LPH, Lipotropin beta, Lipotropin gamma, Lipotropin, included, MSH, Melanocyte-stimulating hormone, included, Melanotropin alpha, Melanotropin beta, Melanotropin gamma, Melanotropin, included, Met-enkephalin, NPP, Opiomelanocortin prepropeptide, POC, POMC, Pomc-1, Pomc2, Pro ACTH endorphin, Pro opiomelanocortin, Pro-opiomelanocortin-alpha, Proopiomelanocortin preproprotein, Tetracosactide, alpha-MSH, beta-MSH, gamma-MSH
Colorimetric
Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
Sandwich (quantitative)
Mouse, Rat
7.81 - 500 pg/mL
1h 30m
= 6 pg/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse/Rat ACTH (adrenocorticotropic hormone) in Heparin Plasma, Citrate plasma, Serum, EDTA Plasma samples.
Alternative names=Adrenocorticotropic hormone, Adrenocorticotropin, Alpha melanocyte stimulating hormone, Beta LPH, Beta melanocyte stimulating hormone, Beta-endorphin, Corticotropin lipotropin, Corticotropin-like intermediary peptide, Gamma LPH, Lipotropin beta, Lipotropin gamma, Lipotropin, included, MSH, Melanocyte-stimulating hormone, included, Melanotropin alpha, Melanotropin beta, Melanotropin gamma, Melanotropin, included, Met-enkephalin, NPP, Opiomelanocortin prepropeptide, POC, POMC, Pomc-1, Pomc2, Pro ACTH endorphin, Pro opiomelanocortin, Pro-opiomelanocortin-alpha, Proopiomelanocortin preproprotein, Tetracosactide, alpha-MSH, beta-MSH, gamma-MSH
Colorimetric
Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
Sandwich (quantitative)
Mouse, Rat
7.81 - 500 pg/mL
1h 30m
Pre-coated microplate (12 x 8 well strips)
= 6 pg/mL
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Plasma | n 8 | mean - | SD - | C.V. 4.2 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Plasma | n 3 | mean - | SD - | C.V. 6.6 |
Sample type | Average % | Range |
---|---|---|
Sample type Serum | Average % = 87 | Range 82 - 92 % |
Sample type EDTA Plasma | Average % = 105 | Range 100 - 110 % |
Sample type Heparin Plasma | Average % = 116 | Range 113 - 120 % |
Sample type Citrate plasma | Average % = 101 | Range 89 - 107 % |
Blue Ice
+4°C
+4°C
Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone) (ab263880) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ACTH (adrenocorticotropic hormone) protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse/Rat ACTH (adrenocorticotropic hormone) with 6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
ACTH, adrenocorticotropic hormone, is a peptide hormone cleavage product of the POMC protein produced by the pituitary gland. ACTH plays an important role in stress and circadian rhythm signaling. ACTH's primary action is on the adrenal cortex, where its binding induces the secretion of glucocorticoid steroid hormones. The peptide sequence for this hormone is highly evolutionarily conserved amongst mammals. Specifically, for mouse and rat, this sequence shares 100% identity.
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