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Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse/Rat ACTH (adrenocorticotropic hormone) in Heparin Plasma, Citrate plasma, Serum, EDTA Plasma samples.

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Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Mouse, Rat

Range

7.81 - 500 pg/mL

Assay time

1h 30m

Sensitivity

= 6 pg/mL

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Shipping To:
Korea, Rep of

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Images

Target data

What's included?

10 x 96 Tests
Components
10X Mouse/Rat ACTH (adrenocorticotropic hormone) Capture Antibody
10 x 600 µL
10X Mouse/Rat ACTH (adrenocorticotropic hormone) Detector Antibody
10 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 200 mL
5X Cell Extraction Buffer PTR (ab193970)
2 x 50 mL
Antibody Diluent 4BI
10 x 6 mL
Mouse/Rat ACTH (adrenocorticotropic hormone) Lyophilized Protein
10 x 2 Vial
Plate Seals
10 x 1 Unit
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
10 x 1 Unit
Stop Solution
1 x 120 mL
TMB Development Solution
1 x 120 mL

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Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse/Rat ACTH (adrenocorticotropic hormone) in Heparin Plasma, Citrate plasma, Serum, EDTA Plasma samples.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Mouse, Rat

Range

7.81 - 500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 6 pg/mL

Precision

Intra assay

Sample
Plasma
n
8
mean
-
SD
-
C.V.
4.2

Inter assay

Sample
Plasma
n
3
mean
-
SD
-
C.V.
6.6

Recovery

Sample specific recovery

Sample type
Serum
Average %
= 87
Range
82 - 92 %
Sample type
EDTA Plasma
Average %
= 105
Range
100 - 110 %
Sample type
Heparin Plasma
Average %
= 116
Range
113 - 120 %
Sample type
Citrate plasma
Average %
= 101
Range
89 - 107 %

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone) (ab263880) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ACTH (adrenocorticotropic hormone) protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse/Rat ACTH (adrenocorticotropic hormone) with 6 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

ACTH, adrenocorticotropic hormone, is a peptide hormone cleavage product of the POMC protein produced by the pituitary gland. ACTH plays an important role in stress and circadian rhythm signaling. ACTH's primary action is on the adrenal cortex, where its binding induces the secretion of glucocorticoid steroid hormones. The peptide sequence for this hormone is highly evolutionarily conserved amongst mammals. Specifically, for mouse and rat, this sequence shares 100% identity.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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