Rat IgG ELISA Kit

Rat IgG ELISA Kit (ab189578) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IgG protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat IgG with 55 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

ASSAY SPECIFICITY

This kit recognizes native rat IgG in serum, plasma and cell culture supernatant samples.

SPECIES REACTIVITY

This kit recognizes rat IgG protein and cross reacts with hamster IgG.

Other species reactivity was determined by measuring 1 to 1 million diluted serum samples of various species, interpolating the protein concentrations from the rat IgG standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in rat serum assayed at the same dilution.

Reactivity < 0.3% was determined for the following species: Mouse, Guinea Pig, Rabbit, Dog, Goat, Pig, Cow, Human, Chicken

Hamster serum displays 71.4% cross-reactvity.

There are four classes of immunoglobulins in rat: IgA, IgE, IgM, and IgG. IgG is the most abundant immunoglobulin and is equally distributed in blood and tissue. In rat, the IgG class is further divided into four subclasses: IgG1, IgG2a, IgG2b, and IgG2c. The general immunoglobulin structure is composed of four polypeptide chains, two heavy and two light chains linked together and to each other by disulfide bonds, creating a tetrameric quaternary structure. The resulting tetramer creates two identical halves which together form a Y like structure. While the amino-terminal portions that exhibits highly variable amino-acid composition are involved in antigen binding, the C terminal constant parts are involved in complement binding, placental passage and binding to cell membrane.

IgG is involved in response to a foreign antigen and the presence of IgG usually signifies a mature antibody response. IgG has a molecular weight of about 150 kDa, can bind to many pathogens and also plays an important role in antibody dependent cell-mediated cytotoxicity. Typically rat serum and plasma samples contain about 7 to 10 mg/ml of IgG.