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Step up your high-throughput screening with our 384-well SimpleStep ELISA kits.
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Troubleshooting for ELISA
Our experts share practical tips and solutions in clear guides to common issues, like weak signal, high background noise, and more.
- Competitive ELISA troubleshooting tips
Solutions to common problems with competitive ELISA.
- High background in ELISA
Usually caused by issues in assay set-up or the wells in your plate.
- Inconsistent results and high coefficient of variation in ELISA
High variance indicates greater inconsistency and error in your results.
- Difficulty in obtaining signal for ELISA
This can range from no to very low or inconsistent signal when signal is expected.
- Problems with standard curve fit in ELISA
Accurate standard curves are needed to determine the concentration of target protein in your sample.
- Blank areas in ELISPOT
Find solutions for blank areas in ELISPOT.
- Blank centre ELISPOT
Find solutions for a blank centre in ELISPOT
- Confluent spots in ELISPOT
Find solutions for confluent spots in ELISPOT.
- False positives in ELISPOT
Find solutions for false positives in ELISPOT.
- High background ELISPOT
Discover solutions for high background in ELISPOT
- Why are there no spots on my ELISPOT?
Find solutions to no spots in ELISPOT.
- Poorly defined spots in ELISPOT
Find solutions for poorly defined spots in ELISPOT.