Cytokine Array - Human Cytokine Antibody Array (Membrane, 42 Targets)
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(47 Publications)
Cytokine Array - Human Cytokine Antibody Array (Membrane, 42 Targets) ab133997 is a membrane based multiplex immunoassay for the simultaneous detection of 42 human cytokines in all sample types.
- More data, less sample: screen multiple cytokines with the same sample
- Global view of cytokine expression
- Chemiluminescent detection - no specific equipment required
- Highly sensitive (pg/mL)
View Alternative Names
RNASE5, ANG, Angiogenin, Ribonuclease 5, RNase 5, MCP1, SCYA2, CCL2, C-C motif chemokine 2, HC11, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, Small-inducible cytokine A2, MCAF, MCP-1, IFNB2, IL6, Interleukin-6, IL-6, B-cell stimulatory factor 2, CTL differentiation factor, Hybridoma growth factor, Interferon beta-2, BSF-2, CDF, IFN-beta-2, C17orf33, GCSF, CSF3, Granulocyte colony-stimulating factor, G-CSF, Pluripoietin, PDGF2, SIS, PDGFB, Platelet-derived growth factor subunit B, PDGF subunit B, PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis, ENA78, SCYB5, CXCL5, C-X-C motif chemokine 5, ENA-78(1-78), Epithelial-derived neutrophil-activating protein 78, Neutrophil-activating peptide ENA-78, Small-inducible cytokine B5, Macrophage colony-stimulating factor 1, CSF-1, M-CSF, MCSF, Lanimostim, Proteoglycan macrophage colony-stimulating factor, PG-M-CSF, CSF1, D17S136E, SCYA5, CCL5, C-C motif chemokine 5, EoCP, Eosinophil chemotactic cytokine, SIS-delta, Small-inducible cytokine A5, T cell-specific protein P228, T-cell-specific protein RANTES, TCP228, SCYA17, TARC, CCL17, C-C motif chemokine 17, CC chemokine TARC, Small-inducible cytokine A17, Thymus and activation-regulated chemokine, MCP2, SCYA10, SCYA8, CCL8, C-C motif chemokine 8, HC14, Monocyte chemoattractant protein 2, Monocyte chemotactic protein 2, Small-inducible cytokine A8, MCP-2, GRO2, GROB, MIP2A, SCYB2, CXCL2, C-X-C motif chemokine 2, Growth-regulated protein beta, Macrophage inflammatory protein 2-alpha, Gro-beta, MIP2-alpha, TGFB, TGFB1, Transforming growth factor beta-1 proprotein, MDC, SCYA22, A-152E5.1, CCL22, C-C motif chemokine 22, CC chemokine STCP-1, MDC(1-69), Macrophage-derived chemokine, Small-inducible cytokine A22, Stimulated T-cell chemotactic protein 1, SCYA1, CCL1, C-C motif chemokine 1, Small-inducible cytokine A1, T lymphocyte-secreted protein I-309, Interleukin-3, IL-3, Hematopoietic growth factor, Mast cell growth factor, Multipotential colony-stimulating factor, P-cell-stimulating factor, MCGF, IL3, Interleukin-2, IL-2, T-cell growth factor, TCGF, IL2, Interleukin-4, IL-4, B-cell stimulatory factor 1, Binetrakin, Lymphocyte stimulatory factor 1, Pitrakinra, BSF-1, IL4, IL1F2, IL1B, Interleukin-1 beta, IL-1 beta, Catabolin, TNFA, TNFSF2, TNF, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Pro-epidermal growth factor, EGF, GMCSF, CSF2, Granulocyte-macrophage colony-stimulating factor, GM-CSF, Colony-stimulating factor, Molgramostin, Sargramostim, CSF, GRO, GRO1, GROA, MGSA, SCYB1, CXCL1, Growth-regulated alpha protein, C-X-C motif chemokine 1, GRO-alpha(1-73), Melanoma growth stimulatory activity, Neutrophil-activating protein 3, NAP-3, GRO3, GROG, SCYB3, CXCL3, C-X-C motif chemokine 3, GRO-gamma(1-73), Growth-regulated protein gamma, Macrophage inflammatory protein 2-beta, GRO-gamma, MIP2-beta, Interferon gamma, IFN-gamma, Immune interferon, IFNG, IBP1, IGF-1, IGF1, Insulin-like growth factor 1, Insulin-like growth factor I, Mechano growth factor, Somatomedin-C, IGF-I, MGF, IL1F1, IL1A, Interleukin-1 alpha, IL-1 alpha, Hematopoietin-1, Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10, NC30, IL13, Interleukin-13, IL-13, Interleukin-15, IL-15, IL15, Interleukin-5, IL-5, B-cell differentiation factor I, Eosinophil differentiation factor, T-cell replacing factor, TRF, IL5, Interleukin-7, IL-7, IL7, IL8, CXCL8, Interleukin-8, IL-8, C-X-C motif chemokine 8, Chemokine (C-X-C motif) ligand 8, Emoctakin, Granulocyte chemotactic protein 1, Monocyte-derived neutrophil chemotactic factor, Monocyte-derived neutrophil-activating peptide, Neutrophil-activating protein 1, Protein 3-10C, T-cell chemotactic factor, GCP-1, MDNCF, MONAP, NAP-1, OB, OBS, LEP, Leptin, Obese protein, Obesity factor, MCP3, SCYA6, SCYA7, CCL7, C-C motif chemokine 7, Monocyte chemoattractant protein 3, Monocyte chemotactic protein 3, NC28, Small-inducible cytokine A7, MCP-3, CMK, MIG, SCYB9, CXCL9, C-X-C motif chemokine 9, Gamma-interferon-induced monokine, Monokine induced by interferon-gamma, Small-inducible cytokine B9, HuMIG, MIP5, NCC3, SCYA15, CCL15, C-C motif chemokine 15, Chemokine CC-2, Leukotactin-1, MIP-1 delta, Macrophage inflammatory protein 5, Mrp-2b, NCC-3, Small-inducible cytokine A15, HCC-2, LKN-1, MIP-5, Oncostatin-M, OSM, MGF, SCF, KITLG, Kit ligand, Mast cell growth factor, Stem cell factor, c-Kit ligand, SDF1, SDF1A, SDF1B, CXCL12, Stromal cell-derived factor 1, SDF-1, hSDF-1, C-X-C motif chemokine 12, Intercrine reduced in hepatomas, Pre-B cell growth-stimulating factor, IRH, hIRH, PBSF, MGDF, THPO, Thrombopoietin, C-mpl ligand, Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, Myeloproliferative leukemia virus oncogene ligand, ML, TNFB, TNFSF1, LTA, Lymphotoxin-alpha, LT-alpha, TNF-beta, Tumor necrosis factor ligand superfamily member 1, VEGF, VEGFA, L-VEGF, Vascular permeability factor, VPF, NKSF1, IL12A, Interleukin-12 subunit alpha, IL-12A, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, NK cell stimulatory factor chain 1, CLMF p35
- Multiplex Protein Detection
Lab
Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 42 Targets) (AB133997)
Quantification of Human Cytokine Array (membrane antibody array).
Human serum from a pooled donor (n=50) sample was diluted to 50% and assayed using ab133997. Mean pixel density was quantified using CCD camera software analysis.
- Multiplex Protein Detection
Lab
Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 42 Targets) (AB133997)
Quantification of Human Cytokine Array (membrane antibody array).
Cells were cultured unstimulated or stimulated with 10 μg/mL PHA. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab133997. Media alone was used as a negative control. Mean pixel density was quantified using CCD camera software analysis.
- Multiplex Protein Detection
Unknown
Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 42 Targets) (AB133997)
Cytokine array testing image - Abreview.
The antibody array was used to determine the change in expression of cytokines related to cell proliferation.
One million Human breast cancer cells (MCF7) were cultured in a 10mm dish for 48 hours (in 8ml of RPMI-1640 medium) and were then treated with 5 mM Compound X (not disclosed) for 48 hours. The Abcam protocol was followed for the rest of the procedure. 1 mL of supernatant of culture medium was used for sample incubation (4°C overnight).
Rating : 4.5/5
This image is courtesy of an anonymous Abreview.
- Multiplex Protein Detection
Lab
Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 42 Targets) (AB133997)
Human serum from a pooled donor (n=50) sample was diluted to 50% and assayed using ab133997.
- Multiplex Protein Detection
Lab
Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 42 Targets) (AB133997)
Multiplex Protein Detection - Human Cytokine Array - Membrane Antibody Array.
Human peripheral blood cells (1x106 cells/mL) were cultured in RPMI media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 mg/mL streptomycin sulfate.
Cells were cultured unstimulated or stimulated with 10 μg/mL PHA. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab133997. Media alone was used as a negative control.
Reactivity data
Product details
ab133997 is a cytokine array to be used for the simultaneous detection of 42 Human cytokines. It is suitable for all sample types.
Targets: ENA-78, GCSF, GM-CSF, GRO, GRO-alpha, I-309, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 p40/p70, IL-13, IL-15, IFN-gamma, MCP-1, MCP-2, MCP-3, MCSF, MDC, MIG, MIP-1delta, RANTES, SCF, SDF-1, TARC, TGF-beta1, TNF-alpha, TNF-beta, EGF, IGF-I, Angiogenin, Oncostatin M, Thrombopoietin, VEGF-A, PDGF BB, Leptin
Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
If you are interested in this cytokine array, cytokine array ab133998, ab133996, ab169817, ab134003, ab134000, ab169804 and ab169805 may also be of interest.
A table listing all of our human membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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Product protocols
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Target data
Additional targets
Publications (47)
Recent publications for all applications. Explore the full list and refine your search
Molecular cancer 24:116 PubMed40241135
2025
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Pituitary 28:37 PubMed40064752
2025
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Cancer immunology, immunotherapy : CII 74:96 PubMed39904796
2025
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Theranostics 15:1966-1986 PubMed39897553
2025
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Life science alliance 8: PubMed39658088
2024
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Cell death & disease 15:879 PubMed39632807
2024
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Advanced materials (Deerfield Beach, Fla.) 36:e2407156 PubMed39543808
2024
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Scientific reports 14:25815 PubMed39468126
2024
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PloS one 19:e0308838 PubMed39163400
2024
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Cancer cell international 24:276 PubMed39103836
2024
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