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ab169817 is for the simultaneous detection of 60 Human Cytokines.

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Images

Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 60 targets) (AB169817), expandable thumbnail
  • Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 60 targets) (AB169817), expandable thumbnail

Publications

Key facts

Assay type
Semi-quantitative
Reactive species
Human
Target
ANG
(See target data below)

Target data

Function

Secreted ribonuclease that can either promote or restrict cell proliferation of target cells, depending on the context (PubMed:12051708, PubMed:1400510, PubMed:19332886, PubMed:20129916, PubMed:21855800, PubMed:23047679, PubMed:23843625, PubMed:2424496, PubMed:2459697, PubMed:2730651, PubMed:27518564, PubMed:28176817, PubMed:29100074, PubMed:29748193, PubMed:3122207, PubMed:32510170, PubMed:38718836, PubMed:8159680, PubMed:8570639, PubMed:8622921, PubMed:9578571). Endocytosed in target cells via its receptor PLXNB2 and translocates to the cytoplasm or nucleus (PubMed:29100074, PubMed:32510170). Under stress conditions, localizes to the cytoplasm and promotes the assembly of stress granules (SGs): specifically cleaves a subset of tRNAs within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs), resulting in translation repression and inhibition of cell proliferation (PubMed:1400510, PubMed:19332886, PubMed:20129916, PubMed:21855800, PubMed:23047679, PubMed:27518564, PubMed:29100074, PubMed:29748193, PubMed:32510170, PubMed:38718836). tiRNas also prevent formation of apoptosome, thereby promoting cell survival (By similarity). Preferentially cleaves RNAs between a pyrimidine and an adenosine residue, suggesting that it cleaves the anticodon loop of tRNA(Ala) (32-UUAGCAU-38) after positions 33 and 36 (PubMed:3289612, PubMed:38718836). Cleaves a subset of tRNAs, including tRNA(Ala), tRNA(Glu), tRNA(Gly), tRNA(Lys), tRNA(Val), tRNA(His), tRNA(Asp) and tRNA(Sec) (PubMed:31582561). Under growth conditions and in differentiated cells, translocates to the nucleus and stimulates ribosomal RNA (rRNA) transcription, including that containing the initiation site sequences of 45S rRNA, thereby promoting cell growth and proliferation (PubMed:12051708, PubMed:15735021, PubMed:27518564, PubMed:29100074, PubMed:8127865). Angiogenin induces vascularization of normal and malignant tissues via its ability to promote rRNA transcription (PubMed:19354288, PubMed:4074709, PubMed:8448182). Involved in hematopoietic stem and progenitor cell (HSPC) growth and survival by promoting rRNA transcription in growth conditions and inhibiting translation in response to stress, respectively (PubMed:27518564). Mediates the crosstalk between myeloid and intestinal epithelial cells to protect the intestinal epithelial barrier integrity: secreted by myeloid cells and promotes intestinal epithelial cells proliferation and survival (PubMed:32510170). Also mediates osteoclast-endothelial cell crosstalk in growing bone: produced by osteoclasts and protects the neighboring vascular cells against senescence by promoting rRNA transcription (By similarity).

Additional Targets

BDNF, CXCL13, BMP4, BMP6, CCL23, CNTF, EGF, CCL11, CCL24, CCL26, FGF6, FGF7, FLT3LG, CX3CL1, CXCL6, GDNF, CSF2, CCL1, IFNG, IGFBP1, IGFBP2, IGFBP4, IGF1, IL10, IL13, IL15, IL16, IL1A, IL1B, IL1RN, IL2, IL3, IL4, IL5, IL6, IL7, LEP, TNFSF14, CCL2, CCL8, CCL7, CCL13, CSF1, CCL22, CXCL9, CCL15, CCL20, PPBP, NTF3, CCL18, PDGFB, CCL5, KITLG, CXCL12, CCL17, TGFB1, TGFB3, TNF, LTA

Alternative names

RNASE5, ANG, Angiogenin, Ribonuclease 5, RNase 5

What's included?

1 x 4 Membranes
Components
1,000X HRP-Conjugated Streptavidin
1 x 50 µL
1X Blocking Buffer
1 x 25 mL
20X Wash Buffer I
1 x 10 mL
20X Wash Buffer II
1 x 10 mL
2X Cell Lysis Buffer
1 x 10 mL
8-Well Incubation Tray (with Lid)
1 x 1 Unit
Biotinylated Antibody Cocktail (C6)
2 x 1 Vial
Detection Buffer C
1 x 1.5 mL
Detection Buffer D
1 x 1.5 mL
Human Cytokine Antibody Array Membranes (C6)
1 x 4 Unit
Plastic sheets
1 x 1 Unit

Recommended products

ab169817 is for the simultaneous detection of 60 Human Cytokines.

Key facts

Assay type
Semi-quantitative
Reactive species
Human
Target
ANG
Applications
Multiplex Protein Detection
Sample types
Saliva, Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture media, Cell culture supernatant, Milk, Serum, Other biological fluids, Whole Blood, Cell Lysate

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

ab169817 is for the simultaneous detection of 60 Human Cytokines. Suitable for all sample types.

Targets: Angiogenin, BDNF, BLC, BMP-4, BMP-6, CK beta 8-1, CNTF, EGF, Eotaxin-1, Eotaxin-2, Eotaxin-3, FGF-6, FGF-7, Flt-3 Ligand, Fractalkine, GCP-2, GDNF, GM-CSF, I-309, IFN-gamma, IGFBP-1, IGFBP-2, IGFBP-4, IGF-1, IL-10, IL-13, IL-15, IL-16, IL-1 alpha, IL-1 beta, IL-1 ra, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, Leptin, Light, MCP-1, MCP-2, MCP-3, MCP-4, M-CSF, MDC, MIG, MIP-1 delta, MIP-3 alpha, NAP-2, NT-3, PARC, PDGF-BB, RANTES, SCF, SDF-1, TARC, TGF beta 1, TGF beta 3, TNF alpha, TNF beta

Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.


If you are interested in this cytokine array, arrays ab133997 and ab133998 may also be of interest. **A table listing all of our human membrane antibody cytokine arrays and other arrays and the analytes they measure is available .**"


Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 60 targets) (ab169817), expandable thumbnail
    This image is courtesy of a customer review submitted by Nicole Blanchette.

    Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 60 targets) (ab169817)

    Left image: Conditioned media from MCF-7 cells cultured in 2D; right image: Conditioned media from MCF-7 cells cultured in 3D"

    The array was used to examine the potential differences in cytokine profile expression in 2D and 3D culture breast cancer cells (MCF-7), which may account for the significant upregulation of hepcidin (an iron export regulatory protein) observed in 3D culture. Experiments were performed according to the manufacturer's instructions for the array, using cell lysate and conditioned media. Samples and biotin-conjugated anti-cytokines were incubated overnight at 4°C. Since many cytokines were detected at low levels when imaged, a comparison between 2D and 3D culture was not conclusive. Conditioned media and cell lysates yielded similar results."

    Rating : 4/5. Product is recommended to anyone seeking information on production of a broad range of cytokines. Product is very easy to use.

    Verified user: Nicole Blanchette, Graduate Assistant, Molecular Biology & Biophysics, UConnHealth, Farmington, CT, USA.

  • Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 60 targets) (ab169817), expandable thumbnail
    This image is courtesy of an anonymous customer review.

    Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 60 targets) (ab169817)

    Left image: Conditioned media from iPSC-derived astrocytes; right image: Media only control.

    Samples were incubated overnight at 4C as recommended and included the large volume wash. Images were captured using CCD camera for the exposure times indicated on the image.

    Rating 5/5. Simple, sensitive and accurate method to detect multiple cytokines and growth factors from a single sample. The membranes were also consistent across the batch which allowed me to test several samples in parallel. Highly recommended.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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