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AB169817

Cytokine Array - Human Cytokine Antibody Array (Membrane, 60 targets)

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(2 Publications)

ab169817 is for the simultaneous detection of 60 Human Cytokines.

View Alternative Names

RNASE5, ANG, Angiogenin, Ribonuclease 5, RNase 5, MCP1, SCYA2, CCL2, C-C motif chemokine 2, HC11, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, Small-inducible cytokine A2, MCAF, MCP-1, AMAC1, DCCK1, MIP4, PARC, SCYA18, CCL18, C-C motif chemokine 18, Alternative macrophage activation-associated CC chemokine 1, CC chemokine PARC, Dendritic cell chemokine 1, Macrophage inflammatory protein 4, Pulmonary and activation-regulated chemokine, Small-inducible cytokine A18, AMAC-1, DC-CK1, MIP-4, Neurotrophic factor BDNF precursor form, proBDNF, Abrineurin, Brain-derived neurotrophic factor, BDNF, BP2, IBP2, IGFBP2, Insulin-like growth factor-binding protein 2, IBP-2, IGF-binding protein 2, IGFBP-2, Glial cell line-derived neurotrophic factor, hGDNF, Astrocyte-derived trophic factor, ATF, GDNF, IFNB2, IL6, Interleukin-6, IL-6, B-cell stimulatory factor 2, CTL differentiation factor, Hybridoma growth factor, Interferon beta-2, BSF-2, CDF, IFN-beta-2, PDGF2, SIS, PDGFB, Platelet-derived growth factor subunit B, PDGF subunit B, PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis, GCP2, SCYB6, CXCL6, C-X-C motif chemokine 6, Chemokine alpha 3, Granulocyte chemotactic protein 2, Small-inducible cytokine B6, CKA-3, GCP-2, CTAP3, CXCL7, SCYB7, TGB1, THBGB1, PPBP, Platelet basic protein, PBP, C-X-C motif chemokine 7, Leukocyte-derived growth factor, Macrophage-derived growth factor, Small-inducible cytokine B7, LDGF, MDGF, Macrophage colony-stimulating factor 1, CSF-1, M-CSF, MCSF, Lanimostim, Proteoglycan macrophage colony-stimulating factor, PG-M-CSF, CSF1, D17S136E, SCYA5, CCL5, C-C motif chemokine 5, EoCP, Eosinophil chemotactic cytokine, SIS-delta, Small-inducible cytokine A5, T cell-specific protein P228, T-cell-specific protein RANTES, TCP228, SCYA17, TARC, CCL17, C-C motif chemokine 17, CC chemokine TARC, Small-inducible cytokine A17, Thymus and activation-regulated chemokine, VGR, BMP6, Bone morphogenetic protein 6, BMP-6, VG-1-related protein, VG-1-R, VGR-1, MCP2, SCYA10, SCYA8, CCL8, C-C motif chemokine 8, HC14, Monocyte chemoattractant protein 2, Monocyte chemotactic protein 2, Small-inducible cytokine A8, MCP-2, Fms-related tyrosine kinase 3 ligand, Flt3 ligand, Flt3L, SL cytokine, FLT3LG, TGFB, TGFB1, Transforming growth factor beta-1 proprotein, Transforming growth factor beta-3 proprotein, TGFB3, IBP4, IGFBP4, Insulin-like growth factor-binding protein 4, IBP-4, IGF-binding protein 4, IGFBP-4, MDC, SCYA22, A-152E5.1, CCL22, C-C motif chemokine 22, CC chemokine STCP-1, MDC(1-69), Macrophage-derived chemokine, Small-inducible cytokine A22, Stimulated T-cell chemotactic protein 1, CD258, HVEML, LIGHT, UNQ391/PRO726, TNFSF14, Tumor necrosis factor ligand superfamily member 14, Herpes virus entry mediator ligand, HVEM-L, Herpesvirus entry mediator ligand, MCP4, NCC1, SCYA13, CCL13, C-C motif chemokine 13, CK-beta-10, Monocyte chemoattractant protein 4, Monocyte chemotactic protein 4, NCC-1, Small-inducible cytokine A13, MCP-4, SCYA1, CCL1, C-C motif chemokine 1, Small-inducible cytokine A1, T lymphocyte-secreted protein I-309, Interleukin-3, IL-3, Hematopoietic growth factor, Mast cell growth factor, Multipotential colony-stimulating factor, P-cell-stimulating factor, MCGF, IL3, Interleukin-2, IL-2, T-cell growth factor, TCGF, IL2, Interleukin-4, IL-4, B-cell stimulatory factor 1, Binetrakin, Lymphocyte stimulatory factor 1, Pitrakinra, BSF-1, IL4, IL1F2, IL1B, Interleukin-1 beta, IL-1 beta, Catabolin, TNFA, TNFSF2, TNF, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, HST2, HSTF2, FGF6, Fibroblast growth factor 6, FGF-6, Heparin secretory-transforming protein 2, Heparin-binding growth factor 6, HST-2, HSTF-2, HBGF-6, BCA1, BLC, SCYB13, CXCL13, C-X-C motif chemokine 13, Angie, B cell-attracting chemokine 1, B lymphocyte chemoattractant, CXC chemokine BLC, Small-inducible cytokine B13, BCA-1, BMP2B, DVR4, BMP4, Bone morphogenetic protein 4, BMP-4, Bone morphogenetic protein 2B, BMP-2B, MIP3, MPIF1, SCYA23, CCL23, C-C motif chemokine 23, CK-beta-8, Macrophage inflammatory protein 3, Myeloid progenitor inhibitory factor 1, Small-inducible cytokine A23, CKB-8, MIP-3, MPIF-1, Ciliary neurotrophic factor, CNTF, Pro-epidermal growth factor, EGF, SCYA11, CCL11, Eotaxin, C-C motif chemokine 11, Eosinophil chemotactic protein, Small-inducible cytokine A11, MPIF2, SCYA24, CCL24, C-C motif chemokine 24, CK-beta-6, Eosinophil chemotactic protein 2, Myeloid progenitor inhibitory factor 2, Small-inducible cytokine A24, Eotaxin-2, MPIF-2, SCYA26, UNQ216/PRO242, CCL26, C-C motif chemokine 26, CC chemokine IMAC, Eotaxin-3, Macrophage inflammatory protein 4-alpha, Small-inducible cytokine A26, Thymic stroma chemokine-1, MIP-4-alpha, TSC-1, KGF, FGF7, Fibroblast growth factor 7, FGF-7, Heparin-binding growth factor 7, Keratinocyte growth factor, HBGF-7, FKN, NTT, SCYD1, A-152E5.2, CX3CL1, Fractalkine, C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, Neurotactin, Small-inducible cytokine D1, GMCSF, CSF2, Granulocyte-macrophage colony-stimulating factor, GM-CSF, Colony-stimulating factor, Molgramostin, Sargramostim, CSF, Interferon gamma, IFN-gamma, Immune interferon, IFNG, IBP1, IGF-1, IGF1, Insulin-like growth factor 1, Insulin-like growth factor I, Mechano growth factor, Somatomedin-C, IGF-I, MGF, IBP1, IGFBP1, Insulin-like growth factor-binding protein 1, IBP-1, IGF-binding protein 1, IGFBP-1, Placental protein 12, PP12, IL1F1, IL1A, Interleukin-1 alpha, IL-1 alpha, Hematopoietin-1, IL1F3, IL1RA, IL1RN, Interleukin-1 receptor antagonist protein, IL-1RN, IL-1ra, IRAP, ICIL-1RA, IL1 inhibitor, Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10, NC30, IL13, Interleukin-13, IL-13, Interleukin-15, IL-15, IL15, Pro-interleukin-16, IL16, Interleukin-5, IL-5, B-cell differentiation factor I, Eosinophil differentiation factor, T-cell replacing factor, TRF, IL5, Interleukin-7, IL-7, IL7, OB, OBS, LEP, Leptin, Obese protein, Obesity factor, MCP3, SCYA6, SCYA7, CCL7, C-C motif chemokine 7, Monocyte chemoattractant protein 3, Monocyte chemotactic protein 3, NC28, Small-inducible cytokine A7, MCP-3, CMK, MIG, SCYB9, CXCL9, C-X-C motif chemokine 9, Gamma-interferon-induced monokine, Monokine induced by interferon-gamma, Small-inducible cytokine B9, HuMIG, MIP5, NCC3, SCYA15, CCL15, C-C motif chemokine 15, Chemokine CC-2, Leukotactin-1, MIP-1 delta, Macrophage inflammatory protein 5, Mrp-2b, NCC-3, Small-inducible cytokine A15, HCC-2, LKN-1, MIP-5, LARC, MIP3A, SCYA20, CCL20, C-C motif chemokine 20, Beta-chemokine exodus-1, CC chemokine LARC, Liver and activation-regulated chemokine, Macrophage inflammatory protein 3 alpha, Small-inducible cytokine A20, MIP-3-alpha, Neurotrophin-3, NT-3, HDNF, Nerve growth factor 2, Neurotrophic factor, NGF-2, NTF3, MGF, SCF, KITLG, Kit ligand, Mast cell growth factor, Stem cell factor, c-Kit ligand, SDF1, SDF1A, SDF1B, CXCL12, Stromal cell-derived factor 1, SDF-1, hSDF-1, C-X-C motif chemokine 12, Intercrine reduced in hepatomas, Pre-B cell growth-stimulating factor, IRH, hIRH, PBSF, TNFB, TNFSF1, LTA, Lymphotoxin-alpha, LT-alpha, TNF-beta, Tumor necrosis factor ligand superfamily member 1

2 Images
Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 60 targets) (AB169817)
  • Multiplex Protein Detection

Unknown

Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 60 targets) (AB169817)

Left image : Conditioned media from MCF-7 cells cultured in 2D; right image : Conditioned media from MCF-7 cells cultured in 3D"

The array was used to examine the potential differences in cytokine profile expression in 2D and 3D culture breast cancer cells (MCF-7), which may account for the significant upregulation of hepcidin (an iron export regulatory protein) observed in 3D culture. Experiments were performed according to the manufacturer's instructions for the array, using cell lysate and conditioned media. Samples and biotin-conjugated anti-cytokines were incubated overnight at 4°C. Since many cytokines were detected at low levels when imaged, a comparison between 2D and 3D culture was not conclusive. Conditioned media and cell lysates yielded similar results."

Rating : 4/5. Product is recommended to anyone seeking information on production of a broad range of cytokines. Product is very easy to use.

Verified user : Nicole Blanchette, Graduate Assistant, Molecular Biology & Biophysics, UConnHealth, Farmington, CT, USA.

This image is courtesy of an Abreview submitted by Nicole Blanchette.

Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 60 targets) (AB169817)
  • Multiplex Protein Detection

Unknown

Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 60 targets) (AB169817)

Left image : Conditioned media from iPSC-derived astrocytes; right image : Media only control.

Samples were incubated overnight at 4C as recommended and included the large volume wash. Images were captured using CCD camera for the exposure times indicated on the image.

Rating 5/5. Simple, sensitive and accurate method to detect multiple cytokines and growth factors from a single sample. The membranes were also consistent across the batch which allowed me to test several samples in parallel. Highly recommended.

This image is courtesy of an anonymous Abreview.

Key facts

Reacts with

Human

Target

ANG

target

Applications

Multiplex Protein Detection

applications

Sample types

Saliva, Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture media, Cell culture supernatant, Milk, Serum, Other biological fluids, Whole Blood, Cell Lysate

Reactivity data

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Product details

ab169817 is for the simultaneous detection of 60 Human Cytokines. Suitable for all sample types.

Targets: Angiogenin, BDNF, BLC, BMP-4, BMP-6, CK beta 8-1, CNTF, EGF, Eotaxin-1, Eotaxin-2, Eotaxin-3, FGF-6, FGF-7, Flt-3 Ligand, Fractalkine, GCP-2, GDNF, GM-CSF, I-309, IFN-gamma, IGFBP-1, IGFBP-2, IGFBP-4, IGF-1, IL-10, IL-13, IL-15, IL-16, IL-1 alpha, IL-1 beta, IL-1 ra, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, Leptin, Light, MCP-1, MCP-2, MCP-3, MCP-4, M-CSF, MDC, MIG, MIP-1 delta, MIP-3 alpha, NAP-2, NT-3, PARC, PDGF-BB, RANTES, SCF, SDF-1, TARC, TGF beta 1, TGF beta 3, TNF alpha, TNF beta

Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.

If you are interested in this cytokine array, arrays ab133997 and ab133998 may also be of interest.

A table listing all of our human membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Product protocols

Target data

Secreted ribonuclease that can either promote or restrict cell proliferation of target cells, depending on the context (PubMed : 12051708, PubMed : 1400510, PubMed : 19332886, PubMed : 20129916, PubMed : 21855800, PubMed : 23047679, PubMed : 23843625, PubMed : 2424496, PubMed : 2459697, PubMed : 2730651, PubMed : 27518564, PubMed : 28176817, PubMed : 29100074, PubMed : 29748193, PubMed : 3122207, PubMed : 32510170, PubMed : 38718836, PubMed : 8159680, PubMed : 8570639, PubMed : 8622921, PubMed : 9578571). Endocytosed in target cells via its receptor PLXNB2 and translocates to the cytoplasm or nucleus (PubMed : 29100074, PubMed : 32510170). Under stress conditions, localizes to the cytoplasm and promotes the assembly of stress granules (SGs) : specifically cleaves a subset of tRNAs within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs), resulting in translation repression and inhibition of cell proliferation (PubMed : 1400510, PubMed : 19332886, PubMed : 20129916, PubMed : 21855800, PubMed : 23047679, PubMed : 27518564, PubMed : 29100074, PubMed : 29748193, PubMed : 32510170, PubMed : 38718836). tiRNas also prevent formation of apoptosome, thereby promoting cell survival (By similarity). Preferentially cleaves RNAs between a pyrimidine and an adenosine residue, suggesting that it cleaves the anticodon loop of tRNA(Ala) (32-UUAGCAU-38) after positions 33 and 36 (PubMed : 3289612, PubMed : 38718836). Cleaves a subset of tRNAs, including tRNA(Ala), tRNA(Glu), tRNA(Gly), tRNA(Lys), tRNA(Val), tRNA(His), tRNA(Asp) and tRNA(Sec) (PubMed : 31582561). Under growth conditions and in differentiated cells, translocates to the nucleus and stimulates ribosomal RNA (rRNA) transcription, including that containing the initiation site sequences of 45S rRNA, thereby promoting cell growth and proliferation (PubMed : 12051708, PubMed : 15735021, PubMed : 27518564, PubMed : 29100074, PubMed : 8127865). Angiogenin induces vascularization of normal and malignant tissues via its ability to promote rRNA transcription (PubMed : 19354288, PubMed : 4074709, PubMed : 8448182). Involved in hematopoietic stem and progenitor cell (HSPC) growth and survival by promoting rRNA transcription in growth conditions and inhibiting translation in response to stress, respectively (PubMed : 27518564). Mediates the crosstalk between myeloid and intestinal epithelial cells to protect the intestinal epithelial barrier integrity : secreted by myeloid cells and promotes intestinal epithelial cells proliferation and survival (PubMed : 32510170). Also mediates osteoclast-endothelial cell crosstalk in growing bone : produced by osteoclasts and protects the neighboring vascular cells against senescence by promoting rRNA transcription (By similarity).
See full target information ANG

Additional targets

BDNF,CXCL13,BMP4,BMP6,CCL23,CNTF,EGF,CCL11,CCL24,CCL26,FGF6,FGF7,FLT3LG,CX3CL1,CXCL6,GDNF,CSF2,CCL1,IFNG,IGFBP1,IGFBP2,IGFBP4,IGF1,IL10,IL13,IL15,IL16,IL1A,IL1B,IL1RN,IL2,IL3,IL4,IL5,IL6,IL7,LEP,TNFSF14,CCL2,CCL8,CCL7,CCL13,CSF1,CCL22,CXCL9,CCL15,CCL20,PPBP,NTF3,CCL18,PDGFB,CCL5,KITLG,CXCL12,CCL17,TGFB1,TGFB3,TNF,LTA

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular cancer 20:107 PubMed34419074

2021

RIPK3 activation induces TRIM28 derepression in cancer cells and enhances the anti-tumor microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Han-Hee Park,Hwa-Ryeon Kim,Sang-Yeong Park,Sung-Min Hwang,Sun Mi Hong,Sangwook Park,Ho Chul Kang,Michael J Morgan,Jong-Ho Cha,Dakeun Lee,Jae-Seok Roe,You-Sun Kim

Cell 180:411-426.e16 PubMed31928844

2020

Modulation of RNA Condensation by the DEAD-Box Protein eIF4A.

Applications

Unspecified application

Species

Unspecified reactive species

Devin Tauber,Gabriel Tauber,Anthony Khong,Briana Van Treeck,Jerry Pelletier,Roy Parker
View all publications
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