AB133993

Cytokine Array - Mouse Cytokine Antibody Array (Membrane, 22 Targets)

Name: Mouse Cytokine Antibody Array (Membrane, 22 Targets) (ab133993) | Abcam
Brand: Abcam Limited
SKU: AB133993
Price: 505 USD
Availability: InStock

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(19 Publications)

Cytokine Array - Mouse Cytokine Antibody Array (Membrane, 22 Targets) ab133993 is a membrane based multiplex immunoassay for the simultaneous detection of 22 mouse cytokines in all sample types.

- More data, less sample: screen multiple cytokines with the same sample
- Global view of cytokine expression
- Chemiluminescent detection - no specific equipment required
- Highly sensitive (pg/mL)

View Alternative Names

Scya5, Ccl5, C-C motif chemokine 5, MuRantes, SIS-delta, Small-inducible cytokine A5, T-cell-specific protein RANTES, CD120a, Tnfr-1, Tnfr1, Tnfrsf1a, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor 1, Tumor necrosis factor receptor type I, p55, p60, TNF-R1, TNF-RI, TNFR-I, Mcp5, Scya12, Ccl12, C-C motif chemokine 12, MCP-1-related chemokine, Monocyte chemoattractant protein 5, Monocyte chemotactic protein 5, Small-inducible cytokine A12, MCP-5, CD131, Aic2b, Csf2rb1, Il3rb1, Csf2rb, Cytokine receptor common subunit beta, GM-CSF/IL-3/IL-5 receptor common beta subunit, Csfg, Csf3, Granulocyte colony-stimulating factor, G-CSF, Interferon gamma, IFN-gamma, Ifng, Interleukin-12 subunit alpha, IL-12A, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, CLMF p35, Il12a, Interleukin-12 subunit beta, IL-12B, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p40, CLMF p40, Il12b, Il-13, Il13, Interleukin-13, IL-13, T-cell activation protein P600, Csfmu, Il-3, Il3, Interleukin-3, IL-3, Hematopoietic growth factor, Mast cell growth factor, Multipotential colony-stimulating factor, P-cell-stimulating factor, MCGF, Il-4, Il4, Interleukin-4, IL-4, B-cell IgG differentiation factor, B-cell growth factor 1, B-cell stimulatory factor 1, IGG1 induction factor, Lymphocyte stimulatory factor 1, BSF-1, Il-5, Il5, Interleukin-5, IL-5, B-cell growth factor II, Cytotoxic T-lymphocyte inducer, Eosinophil differentiation factor, T-cell replacing factor, BCGF-II, TRF, Il-6, Il6, Interleukin-6, IL-6, B-cell hybridoma growth factor, Interleukin HP-1, Interleukin-9, IL-9, Cytokine P40, T-cell growth factor P40, Il9, Kitl, Mgf, Sl, Slf, Kitlg, Kit ligand, Hematopoietic growth factor KL, Mast cell growth factor, Steel factor, Stem cell factor, c-Kit ligand, MGF, SCF, Thrombopoietin, C-mpl ligand, Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, Myeloproliferative leukemia virus oncogene ligand, ML, MGDF, Thpo, Vegf, Vegfa, L-VEGF, Vascular permeability factor, VPF, Ctla8, Il17, Il17a, Interleukin-17A, IL-17, IL-17A, Cytotoxic T-lymphocyte-associated antigen 8, CTLA-8, Tnfa, Tnfsf2, Tnf, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Je, Mcp1, Scya2, Ccl2, C-C motif chemokine 2, Monocyte chemoattractant protein 1, Monocyte chemotactic protein 1, Platelet-derived growth factor-inducible protein JE, Small-inducible cytokine A2, MCP-1, Il-10, Il10, Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, Il-2, Il2, Interleukin-2, IL-2, T-cell growth factor, TCGF

1 Images

Multiplex Protein Detection - Cytokine Array - Mouse Cytokine Antibody Array (Membrane, 22 Targets) (AB133993)

Multiplex Protein Detection

Unknown

Multiplex Protein Detection - Cytokine Array - Mouse Cytokine Antibody Array (Membrane, 22 Targets) (AB133993)

The mouse cytokine antibody array containing 22 target proteins was used to determine the effect of an anti-inflammatory synthetic peptide (AISP) on the induction of cytokine production in mouse macrophages Raw264.7. Three million macrophages were plated in two sets in a six well plate and used in the study; one set was treated with vehicle alone while the other set was treated with the AISP at 20 μg/mL for 5 h. After 5 h of AISP treatment, the conditioned medium were collected and centrifuged at 14,000 rpm for 10 min and the supernatant was collected in a pre-cooled tube and stored overnight at 4 °C. The experiment was performed following the manufacturer's instructions. All incubations were performed at 4°C for 12 h. After incubation and washing steps, the proteins were detected using the provided detection reagents. Membranes were exposed for 15 s. The representative membranes for non-AISP and the AISP treated macrophages are shown.

Abreview rating 4/5 stars. Review from Abcam user community. Verified customer - Yi Chen, Ph.D. Research Fellow, Spiegelman Lab, Dana-Farber Cancer Institute, Department of Cancer Biology, Boston, USA. Submitted 25-Jun-15.

This image is courtesy of an Abreview submitted by M.C Alejandro Huante-Mendoza and Dr. Víctor Manuel Baizabal-Aguirre

Key facts

Reacts with

Mouse

Target

Ccl5

target

Applications

Multiplex Protein Detection

applications

Sample types

Saliva, Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture media, Cell culture supernatant, Milk, Serum, Other biological fluids, Whole Blood, Cell Lysate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Multiplex Protein Detection": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

ab133993 is for simultaneous detection of 22 Mouse Cytokines. Suitable for all sample types.

Targets: GCSF, GM-CSF, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12p70, IL-13, IL-17, IFN-gamma, MCP-1, MCP-5, RANTES, SCF, sTNF RI, TNF-alpha, Thrombopoietin, VEGF

Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.

If you are interested in this cytokine array, a table of mouse cytokine membrane arrays and the analytes they measure is available here.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Product protocols

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Target data

Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. May also be an agonist of the G protein-coupled receptor GPR75. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells.

See full target information Ccl5

Additional targets

Csf2rb,Il10,Il2,Ccl12,Ccl2,Csf3,Ifng,Il12a,Il12b,Il13,Il3,Il4,Il17a,Il5,Il6,Il9,Kitlg,Thpo,Tnfrsf1a,Vegfa,Tnf

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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Brain sciences 14: PubMed38790446

2024

B355252 Suppresses LPS-Induced Neuroinflammation in the Mouse Brain.

Applications

Unspecified application

Species

Unspecified reactive species

Qingping He,Qi Qi,Gordon C Ibeanu,P Andy Li

Cellular and molecular gastroenterology and hepatology 17:965-981 PubMed38342302

2024

Myeloid Deletion of Cdc42 Protects Liver From Hepatic Ischemia-Reperfusion Injury via Inhibiting Macrophage-Mediated Inflammation in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jing He,Meng-Yu Tang,Li-Xin Liu,Chen-Xian Kong,Wen Chen,Lu Wang,Shao-Bin Zhi,Hong-Wei Sun,Yu-Chun Huang,Guo-Yu Chen,Hong-Bo Xin,Ke-Yu Deng

Toxics 11: PubMed37888727

2023

Acetyl Tributyl Citrate Exposure at Seemingly Safe Concentrations Induces Adverse Effects in Different Genders of Type 2 Diabetes Mice, Especially Brain Tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Yuchao Zhang,Zhihuai Zhang,Sijie Zhu,Liangyu Liu,Xudong Liu,Xu Yang

Pharmaceutics 15: PubMed37376217

2023

Arginine-Rich Cell-Penetrating Peptide-Mediated Transduction of Mouse Nasal Cells with FOXP3 Protein Alleviates Allergic Rhinitis.

Applications

Unspecified application

Species

Unspecified reactive species

Toru Miwa,Yumi Takemiya,Kazuki Amesara,Hiroko Kawai,Yuichi Teranishi

Experimental & molecular medicine 55:520-531 PubMed36854775

2023

TGFBI remodels adipose metabolism by regulating the Notch-1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Seul Gi Lee,Jongbeom Chae,Seon Min Woo,Seung Un Seo,Ha-Jeong Kim,Sang-Yeob Kim,David D Schlaepfer,In-San Kim,Hee-Sae Park,Taeg Kyu Kwon,Ju-Ock Nam

Frontiers in cardiovascular medicine 9:1075358 PubMed36741841

2023

Electrical, structural, and autonomic atrial remodeling underlies atrial fibrillation in inflammatory atrial cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshiko Murakata,Fumi Yamagami,Nobuyuki Murakoshi,DongZhu Xu,Zhonghu Song,Siqi Li,Yuta Okabe,Kazuhiro Aonuma,ZiXun Yuan,Haruka Mori,Kazutaka Aonuma,Kazuko Tajiri,Masaki Ieda

Frontiers in physiology 13:900961 PubMed36045744

2022

Finasteride Alleviates High Fat Associated Protein-Overload Nephropathy by Inhibiting Trimethylamine N-Oxide Synthesis and Regulating Gut Microbiota.

Applications

Unspecified application

Species

Unspecified reactive species

Zuoyuan Wang,Li You,Yuan Ren,Xiaoye Zhu,Xiaoyi Mao,Xiaowan Liang,Tingting Wang,Yumeng Guo,Te Liu,Jun Xue

Cancer research 82:885-899 PubMed34965936

2021

Single-Cell Analysis Unveils the Role of the Tumor Immune Microenvironment and Notch Signaling in Dormant Minimal Residual Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Mahnaz Janghorban,Yuchen Yang,Na Zhao,Clark Hamor,Tuan M Nguyen,Xiang H-F Zhang,Jeffrey M Rosen

Scientific reports 11:22009 PubMed34759307

2021

ASKA technology-based pull-down method reveals a suppressive effect of ASK1 on the inflammatory NOD-RIPK2 pathway in brown adipocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Saki Takayanagi,Kengo Watanabe,Takeshi Maruyama,Motoyuki Ogawa,Kazuhiro Morishita,Mayumi Soga,Tomohisa Hatta,Tohru Natsume,Tomoya Hirano,Hiroyuki Kagechika,Kazuki Hattori,Isao Naguro,Hidenori Ichijo

Nature cancer 2:444-456 PubMed33899001

2021

EZH2 inhibition activates a dsRNA-STING-interferon stress axis that potentiates response to PD-1 checkpoint blockade in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Katherine L Morel,Anjali V Sheahan,Deborah L Burkhart,Sylvan C Baca,Nadia Boufaied,Yin Liu,Xintao Qiu,Israel Cañadas,Kevin Roehle,Max Heckler,Carla Calagua,Huihui Ye,Constantia Pantelidou,Phillip Galbo,Sukanya Panja,Antonina Mitrofanova,Scott Wilkinson,Nichelle C Whitlock,Shana Y Trostel,Anis A Hamid,Adam S Kibel,David A Barbie,Atish D Choudhury,Mark M Pomerantz,Christopher J Sweeney,Henry W Long,David J Einstein,Geoffrey I Shapiro,Stephanie K Dougan,Adam G Sowalsky,Housheng Hansen He,Matthew L Freedman,Steven P Balk,Massimo Loda,David P Labbé,Brian M Olson,Leigh Ellis

View all publications

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Cytokine Array - Mouse Cytokine Antibody Array (Membrane, 22 Targets)

Multiplex Protein Detection - Cytokine Array - Mouse Cytokine Antibody Array (Membrane, 22 Targets) (AB133993)

Key facts

Reacts with

Target

Applications

Sample types

Complementary Products

Reagents, Controls & Accessories

Secondary Antibodies

Multiplex Kits

Multiplex Kits

Multiplex Kits

Reactivity data

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Additional targets

Publications (19)

Product promise