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AB133994

Cytokine Array - Mouse Cytokine Antibody Array (Membrane, 32 Targets)

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(4 Publications)

Cytokine Array - Mouse Cytokine Antibody Array (Membrane, 32 Targets) ab133994 is a membrane based multiplex immunoassay for the simultaneous detection of 32 mouse cytokines in all sample types.

- More data, less sample: screen multiple cytokines with the same sample
- Global view of cytokine expression
- Chemiluminescent detection - no specific equipment required
- Highly sensitive (pg/mL)

View Alternative Names

Scya11, Ccl11, Eotaxin, C-C motif chemokine 11, Eosinophil chemotactic protein, Small-inducible cytokine A11, CD120a, Tnfr-1, Tnfr1, Tnfrsf1a, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor 1, Tumor necrosis factor receptor type I, p55, p60, TNF-R1, TNF-RI, TNFR-I, Mip1a, Scya3, Ccl3, C-C motif chemokine 3, Heparin-binding chemotaxis protein, L2G25B, Macrophage inflammatory protein 1-alpha, SIS-alpha, Small-inducible cytokine A3, TY-5, MIP-1-alpha, Mcp5, Scya12, Ccl12, C-C motif chemokine 12, MCP-1-related chemokine, Monocyte chemoattractant protein 5, Monocyte chemotactic protein 5, Small-inducible cytokine A12, MCP-5, Scya21, Scya21b, Ccl21b, C-C motif chemokine 21b, 6Ckine, Beta-chemokine exodus-2, Small-inducible cytokine A21b, Thymus-derived chemotactic agent 4, TCA4, CD131, Aic2b, Csf2rb1, Il3rb1, Csf2rb, Cytokine receptor common subunit beta, GM-CSF/IL-3/IL-5 receptor common beta subunit, Tarc, Ccl17, C-C motif chemokine 17, ABCD-2, CC chemokine TARC, Small-inducible cytokine A17, Thymus and activation-regulated chemokine, Elc, Scya19, Ccl19, C-C motif chemokine 19, Epstein-Barr virus-induced molecule 1 ligand chemokine, Small-inducible cytokine A19, EBI1 ligand chemokine, ELC, Ilc, Scya27, Ccl27, C-C motif chemokine 27, CC chemokine ILC, Cutaneous T-cell-attracting chemokine, ESkine, IL-11 R-alpha-locus chemokine, Skinkine, Small-inducible cytokine A27, CTACK, ALP, mILC, Scya5, Ccl5, C-C motif chemokine 5, MuRantes, SIS-delta, Small-inducible cytokine A5, T-cell-specific protein RANTES, Csfg, Csf3, Granulocyte colony-stimulating factor, G-CSF, Gro, Gro1, Mgsa, Scyb1, Cxcl1, Growth-regulated alpha protein, C-X-C motif chemokine 1, Platelet-derived growth factor-inducible protein KC, Secretory protein N51, Mip-2, Mip2, Scyb2, Cxcl2, C-X-C motif chemokine 2, Macrophage inflammatory protein 2, MIP2, Interferon gamma, IFN-gamma, Ifng, Interleukin-12 subunit alpha, IL-12A, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, CLMF p35, Il12a, Interleukin-12 subunit beta, IL-12B, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p40, CLMF p40, Il12b, Il-13, Il13, Interleukin-13, IL-13, T-cell activation protein P600, Csfmu, Il-3, Il3, Interleukin-3, IL-3, Hematopoietic growth factor, Mast cell growth factor, Multipotential colony-stimulating factor, P-cell-stimulating factor, MCGF, Il-4, Il4, Interleukin-4, IL-4, B-cell IgG differentiation factor, B-cell growth factor 1, B-cell stimulatory factor 1, IGG1 induction factor, Lymphocyte stimulatory factor 1, BSF-1, Il-5, Il5, Interleukin-5, IL-5, B-cell growth factor II, Cytotoxic T-lymphocyte inducer, Eosinophil differentiation factor, T-cell replacing factor, BCGF-II, TRF, Il-6, Il6, Interleukin-6, IL-6, B-cell hybridoma growth factor, Interleukin HP-1, Interleukin-9, IL-9, Cytokine P40, T-cell growth factor P40, Il9, Kitl, Mgf, Sl, Slf, Kitlg, Kit ligand, Hematopoietic growth factor KL, Mast cell growth factor, Steel factor, Stem cell factor, c-Kit ligand, MGF, SCF, Ob, Lep, Leptin, Obesity factor, Thrombopoietin, C-mpl ligand, Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, Myeloproliferative leukemia virus oncogene ligand, ML, MGDF, Thpo, Timp, Timp-1, Timp1, Metalloproteinase inhibitor 1, Collagenase inhibitor 16C8 fibroblast, Erythroid-potentiating activity, TPA-S1, TPA-induced protein, Tissue inhibitor of metalloproteinases 1, EPA, TIMP-1, Vegf, Vegfa, L-VEGF, Vascular permeability factor, VPF, Tnfa, Tnfsf2, Tnf, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Je, Mcp1, Scya2, Ccl2, C-C motif chemokine 2, Monocyte chemoattractant protein 1, Monocyte chemotactic protein 1, Platelet-derived growth factor-inducible protein JE, Small-inducible cytokine A2, MCP-1, Il-10, Il10, Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, Il-2, Il2, Interleukin-2, IL-2, T-cell growth factor, TCGF

1 Images
Multiplex Protein Detection - Cytokine Array - Mouse Cytokine Antibody Array (Membrane, 32 Targets) (AB133994)
  • Multiplex Protein Detection

Unknown

Multiplex Protein Detection - Cytokine Array - Mouse Cytokine Antibody Array (Membrane, 32 Targets) (AB133994)

The cytokine antibody array was used to detect changes in cytokine/chemokine secretion by cultured mouse eosinophils treated with recombinant protein of interest or with vehicle control. Briefly, mouse primary eosinophils were harvested and cultured in suspension, 300ng/ml recombinant protein X (our protein of interest) or vehicle alone were added to the cells for 24 hours. Then the conditioned medium were harvested by centrifugation and added directly without dilution onto the cytokine antibody array (the array was first blocked according to manufacturer's protocol). Manufacturer's protocol was followed for the subsequent steps and the end result is shown in the following picture. The amount of three candidate cytokine/chemokines were identified to have changed in the treated conditioned medium : GCSF (H1 and H2), Leptin (K3 and K4), and KC (J3 and J4). The changes of Leptin and KC secretion were subsequently confirmed by ELISA in independent experiments.

Abreview rating 4/5 stars. Review from Abcam user community. Verified customers - Yi Chen, Ph.D. Research Fellow, Spiegelman Lab, Dana-Farber Cancer Institute, Department of Cancer Biology, Boston, USA. Submitted 25-Jun-15.

This image is courtesy of an Abreview submitted by Yi Chen, Ph.D.

Key facts

Reacts with

Mouse

Target

Ccl11

target

Applications

Multiplex Protein Detection

applications

Sample types

Saliva, Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture media, Cell culture supernatant, Milk, Serum, Other biological fluids, Whole Blood, Cell Lysate

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Multiplex Protein Detection": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Product details

ab133994 is for simultaneous detection of 32 Mouse Cytokines. Suitable for all sample types.

Targets: 6Ckine, CTACK, Eotaxin, GCSF, GM-CSF, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p40/p70, IL-12 p70, IL-13, IL-17, IFN-gamma, KC/CXCL1, Leptin/OB, MCP-1, MCP-5, MIP-1alpha, MIP-2, MIP-3beta, RANTES, SCF, sTNF RI, TARC, TIMP-1, TNF-alpha, Thrombopoietin, VEGF-A

Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.

If you are interested in this cytokine array, a table listing all of our mouse membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Product protocols

Target data

Chemokine that plays a central role in both allergic and non-allergic inflammatory reactions by inducing the migration of different leukocyte types including eosinophils, basophils, macrophages and dendritic cells (PubMed : 7568052, PubMed : 8574847). Exerts its effects primarily by binding to the CCR3 receptor. Induces also chemotaxis of endothelial cells and promotes angiogenesis by activating the PI3K/Akt pathway.
See full target information Ccl11

Additional targets

Ccl21b,Csf2rb,Il10,Tnf,Il2,Ccl12,Ccl2,Ccl17,Ccl19,Ccl27,Ccl5,Csf3,Ccl3,Cxcl1,Cxcl2,Ifng,Il12a,Il12b,Il13,Il3,Il4,Il5,Il6,Il9,Kitlg,Lep,Thpo,Timp1,Tnfrsf1a,Vegfa

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 15:4673-4692 PubMed40225565

2025

A new nano-encapsulated TSPO ligand reduces neuroinflammation and improves cognitive functions in Alzheimer's disease model.

Applications

Unspecified application

Species

Unspecified reactive species

Antonella Casamassa,Paola Brancaccio,Virginia Campani,Angela Corvino,Serenella Anzilotti,Giovanni Pecoraro,Giorgia Andreozzi,Raffaella Daniele,Ilaria Piccialli,Anna Pannaccione,Pierluigi Reveglia,Lucia Lecce,Carmela Paolillo,Ferdinando Fiorino,Giuseppe De Rosa,Giuseppe Caliendo,Lucio Annunziato,Giuseppe Pignataro

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2305842 PubMed37967351

2023

A Novel Primary Cilium-Mediated Mechanism Through which Osteocytes Regulate Metastatic Behavior of Both Breast and Prostate Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Stefaan W Verbruggen,Joanne Nolan,Michael P Duffy,Oliver M T Pearce,Christopher R Jacobs,Martin M Knight

The Journal of experimental medicine 220: PubMed36828390

2023

Pleiotrophin drives a prometastatic immune niche in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Debolina Ganguly,Marcel O Schmidt,Morgan Coleman,Tuong-Vi Cindy Ngo,Noah Sorrelle,Adrian T A Dominguez,Gilbert Z Murimwa,Jason E Toombs,Cheryl Lewis,Yisheng V Fang,Fatima Valdes-Mora,David Gallego-Ortega,Anton Wellstein,Rolf A Brekken

Cancer cell 39:423-437.e7 PubMed33450198

2021

Cathepsin C promotes breast cancer lung metastasis by modulating neutrophil infiltration and neutrophil extracellular trap formation.

Applications

Unspecified application

Species

Unspecified reactive species

Yansen Xiao,Min Cong,Jiatao Li,Dasa He,Qiuyao Wu,Pu Tian,Yuan Wang,Shuaixi Yang,Chenxi Liang,Yajun Liang,Jili Wen,Yingjie Liu,Wenqian Luo,Xianzhe Lv,Yunfei He,Dong-Dong Cheng,Tianhao Zhou,Wenjing Zhao,Peiyuan Zhang,Xue Zhang,Yichuan Xiao,Youcun Qian,Hongxia Wang,Qiang Gao,Qing-Cheng Yang,Qifeng Yang,Guohong Hu
View all publications
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