Skip to main content

ab133994 is for simultaneous detection of 32 Mouse Cytokines.

Be the first to review this product! Submit a review

Images

Multiplex Protein Detection - Cytokine Array - Mouse Cytokine Antibody Array (Membrane, 32 Targets) (AB133994), expandable thumbnail

Publications

Key facts

Assay type
Semi-quantitative
Reactive species
Mouse
Target
Ccl11
(See target data below)

Target data

Function

In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils (a prominent feature of allergic inflammatory reactions), but not lymphocytes, macrophages or neutrophils (PubMed:7568052, PubMed:8574847). Binds to CCR3 (By similarity).

Additional Targets

Ccl21b, Csf2rb, Ccl3, Il10, Tnf, Il2, Ccl12, Ccl2, Ccl17, Ccl3, Il10, Tnf, Il2, Ccl12, Ccl2, Ccl17, Ccl19, Ccl27, Ccl5, Csf3, Cxcl1, Cxcl2, Ifng, Il12a, Il12b, Il13, Il3, Il4, Il5, Il6, Il9, Kitlg, Lep, Thpo, Timp1, Tnfrsf1a, Thpo, Vegfa

Alternative names

Scya11, Ccl11, Eotaxin, C-C motif chemokine 11, Eosinophil chemotactic protein, Small-inducible cytokine A11

What's included?

1 x 4 Membranes
Components
1,000X HRP-Conjugated Streptavidin
1 x 50 µL
1X Blocking Buffer
1 x 25 mL
20X Wash Buffer I
1 x 10 mL
20X Wash Buffer II
1 x 10 mL
2X Cell Lysis Buffer
1 x 10 mL
8-Well Incubation Tray (with Lid)
1 x 1 Unit
Biotin-Conjugated Anti-Cytokines
2 x 1 Vial
Cytokine Antibody Array Membranes
1 x 4 Unit
Detection Buffer C
1 x 1.5 mL
Detection Buffer D
1 x 1.5 mL
Plastic sheets
1 x 1 Unit

Recommended products

ab133994 is for simultaneous detection of 32 Mouse Cytokines.

Key facts

Assay type
Semi-quantitative
Reactive species
Mouse
Target
Ccl11
Applications
Multiplex Protein Detection
Sample types
Saliva, Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture media, Cell culture supernatant, Milk, Serum, Other biological fluids, Whole Blood, Cell Lysate

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

ab133994 is for simultaneous detection of 32 Mouse Cytokines. Suitable for all sample types.

Targets: 6Ckine, CTACK, Eotaxin, GCSF, GM-CSF, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p40/p70, IL-12 p70, IL-13, IL-17, IFN-gamma, KC/CXCL1, Leptin/OB, MCP-1, MCP-5, MIP-1alpha, MIP-2, MIP-3beta, RANTES, SCF, sTNF RI, TARC, TIMP-1, TNF-alpha, Thrombopoietin, VEGF-A

Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.


**If you are interested in this cytokine array, a table listing all of our mouse membrane antibody cytokine arrays and other arrays and the analytes they measure is available .**


Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Multiplex Protein Detection - Cytokine Array - Mouse Cytokine Antibody Array (Membrane, 32 Targets) (ab133994), expandable thumbnail
    This image is courtesy of a customer review submitted by Yi Chen, Ph.D.

    Multiplex Protein Detection - Cytokine Array - Mouse Cytokine Antibody Array (Membrane, 32 Targets) (ab133994)

    The cytokine antibody array was used to detect changes in cytokine/chemokine secretion by cultured mouse eosinophils treated with recombinant protein of interest or with vehicle control. Briefly, mouse primary eosinophils were harvested and cultured in suspension, 300ng/ml recombinant protein X (our protein of interest) or vehicle alone were added to the cells for 24 hours. Then the conditioned medium were harvested by centrifugation and added directly without dilution onto the cytokine antibody array (the array was first blocked according to manufacturer's protocol). Manufacturer's protocol was followed for the subsequent steps and the end result is shown in the following picture. The amount of three candidate cytokine/chemokines were identified to have changed in the treated conditioned medium: GCSF (H1 and H2), Leptin (K3 and K4), and KC (J3 and J4). The changes of Leptin and KC secretion were subsequently confirmed by ELISA in independent experiments.

    Abreview rating 4/5 stars. Review from Abcam user community. Verified customers - Yi Chen, Ph.D. Research Fellow, Spiegelman Lab, Dana-Farber Cancer Institute, Department of Cancer Biology, Boston, USA. Submitted 25-Jun-15.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com