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ab133995 is for simultaneous detection of 62 Mouse Cytokines.

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Multiplex Protein Detection - Cytokine Array - Mouse Cytokine Antibody Array (Membrane, 62 Targets) (AB133995), expandable thumbnail

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Key facts

Assay type
Semi-quantitative
Reactive species
Mouse
Target
Axl
(See target data below)

Target data

Function

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, AXL binds and induces tyrosine phosphorylation of PI3-kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TNS2. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Also plays an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response.

Additional Targets

Csf2rb, Tnfrsf1b, Ccl3, Ccl1, Il10, Sell, Cxcl16, Ccl9, Il17a, Lepr, Tnf, Il2, Igfbp6, Cd40, Ccl12, Ccl2, Ccl17, Tnfrsf1b, Ccl3, Ccl1, Il10, Sell, Cxcl16, Ccl9, Il17a, Lepr, Tnf, Il2, Igfbp6, Cd40, Ccl12, Ccl2, Ccl17, Ccl11, Ccl19, Ccl20, Ccl24, Ccl25, Ccl27, Ccl5, Csf1, Csf3, Cx3cl1, Cxcl1, Cxcl10, Cxcl12, Cxcl13, Cxcl2, Cxcl5, Cxcl9, Faslg, Ifng, Igfbp3, Igfbp5, Il12a, Il12b, Il13, IL1A_MOUSE, Il1b, Il3, Il4, Il5, Il6, Il9, Kitlg, Lep, Pf4, Selp, Thpo, Timp1, Tnfrsf8, Tnfsf8, Vcam1, Vegfa, Xcl1, Tnfrsf1a

Alternative names

Ark, Ufo, Axl, Tyrosine-protein kinase receptor UFO, Adhesion-related kinase

What's included?

1 x 8 Membranes
Components
1,000X HRP-Conjugated Streptavidin
1 x 50 µL
1X Blocking Buffer
2 x 25 mL
20X Wash Buffer I
1 x 20 mL
20X Wash Buffer II
1 x 20 mL
2X Cell Lysis Buffer
1 x 16 mL
8-Well Incubation Tray (with Lid)
1 x 1 Unit
Biotinylated Antibody Cocktail (C3)
4 x 1 Vial
Detection Buffer C
1 x 2.5 mL
Detection Buffer D
1 x 2.5 mL
Mouse Cytokine Antibody Arrays Membranes (C3)
2 x 4 Unit
Plastic sheets
1 x 1 Unit

Recommended products

ab133995 is for simultaneous detection of 62 Mouse Cytokines.

Key facts

Assay type
Semi-quantitative
Reactive species
Mouse
Target
Axl
Applications
Multiplex Protein Detection
Sample types
Saliva, Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture media, Cell culture supernatant, Milk, Serum, Other biological fluids, Whole Blood, Cell Lysate

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

ab133995 is for simultaneous detection of 62 Mouse Cytokines. Suitable for all sample types.

Targets: Axl, BLC, CD30 L, CD30, CD40, CRG-2, CTACK, CXCL16, Eotaxin, Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CSF, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1beta, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, IL-1alpha, IL-2, IL-3, IL-3 Rbeta, IL-4, IL-5, IL-6, IL-9, KC/CXCL1, Leptin/OB, Leptin R, LIX, L-Selectin, Lymphotactin, MCP1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3beta, MIP-3alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1alpha, sTNF RI, sTNF RII, TARC, TCA-3, TECK, TIMP-1, TNFalpha, Thrombopoietin, VCAM-1, VEGF-A

Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.


**If you are interested in this cytokine array, a table listing all of our mouse membrane antibody cytokine arrays and other arrays and the analytes they measure is available .**


Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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1 product image

  • Multiplex Protein Detection - Cytokine Array - Mouse Cytokine Antibody Array (Membrane, 62 Targets) (ab133995), expandable thumbnail

    Multiplex Protein Detection - Cytokine Array - Mouse Cytokine Antibody Array (Membrane, 62 Targets) (ab133995)

    Typical results obtained with Abcam Human Cytokine Membrane Antibody Array. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at RT for 1 min.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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