AB133995

Cytokine Array - Mouse Cytokine Antibody Array (Membrane, 62 Targets)

Name: Mouse Cytokine Antibody Array (Membrane, 62 Targets) (ab133995) | Abcam
Brand: Abcam Limited
SKU: AB133995
Price: 840 USD
Availability: InStock

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(12 Publications)

Cytokine Array - Mouse Cytokine Antibody Array (Membrane, 62 Targets) ab133995 is a membrane based multiplex immunoassay for the simultaneous detection of 62 mouse cytokines in all sample types.

- More data, less sample: screen multiple cytokines with the same sample
- Global view of cytokine expression
- Chemiluminescent detection - no specific equipment required
- Highly sensitive (pg/mL)

View Alternative Names

Ark, Ufo, Axl, Tyrosine-protein kinase receptor UFO, Adhesion-related kinase

1 Images

Multiplex Protein Detection - Cytokine Array - Mouse Cytokine Antibody Array (Membrane, 62 Targets) (AB133995)

Multiplex Protein Detection

Unknown

Multiplex Protein Detection - Cytokine Array - Mouse Cytokine Antibody Array (Membrane, 62 Targets) (AB133995)

Typical results obtained with Abcam Human Cytokine Membrane Antibody Array. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at RT for 1 min.

Key facts

Assay type

Semi-quantitative

Reacts with

Mouse

Target

Axl

target

Applications

Multiplex Protein Detection

applications

Sample types

Saliva, Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture media, Cell culture supernatant, Milk, Serum, Other biological fluids, Whole Blood, Cell Lysate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Multiplex Protein Detection": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

ab133995 is for simultaneous detection of 62 Mouse Cytokines. Suitable for all sample types.

Targets: Axl, BLC, CD30 L, CD30, CD40, CRG-2, CTACK, CXCL16, Eotaxin, Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CSF, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1beta, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, IL-1alpha, IL-2, IL-3, IL-3 Rbeta, IL-4, IL-5, IL-6, IL-9, KC/CXCL1, Leptin/OB, Leptin R, LIX, L-Selectin, Lymphotactin, MCP1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3beta, MIP-3alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1alpha, sTNF RI, sTNF RII, TARC, TCA-3, TECK, TIMP-1, TNFalpha, Thrombopoietin, VCAM-1, VEGF-A

Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.

If you are interested in this cytokine array, a table listing all of our mouse membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Product protocols

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Target data

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, AXL binds and induces tyrosine phosphorylation of PI3-kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TNS2. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Also plays an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response.

See full target information Axl

Additional targets

Csf2rb,Tnfrsf1b,Tnf,Il2,Igfbp6,Cd40,Ccl12,Ccl2,Ccl17,Ccl3,Ccl1,Il10,Sell,Cxcl16,Ccl9,Il17a,Lepr,Ccl11,Ccl19,Ccl20,Ccl24,Ccl25,Ccl27,Ccl5,Csf1,Csf3,Cx3cl1,Cxcl1,Cxcl10,Cxcl12,Cxcl13,Cxcl2,Cxcl5,Cxcl9,Faslg,Ifng,Igfbp3,Igfbp5,Il12a,Il12b,Il13,IL1A_MOUSE,Il1b,Il3,Il4,Il5,Il6,Il9,Kitlg,Lep,Pf4,Selp,Thpo,Timp1,Tnfrsf8,Tnfsf8,Vcam1,Vegfa,Xcl1,Tnfrsf1a

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:5927 PubMed39966413

2025

Impact of short wavelength light exposure on body weight, mobility, anxiety like behaviour and cytokine expression.

Applications

Unspecified application

Species

Unspecified reactive species

Heba Al-Hussaini,Mohammed Al-Onaizi,Bara S Abed,Michael B Powner,Sonia M Hasan,Glen Jeffery

Journal of lipid research 65:100620 PubMed39151591

2024

Matrix stiffness regulates the triad communication of adipocytes/macrophages/endothelial cells through CXCL13.

Applications

Unspecified application

Species

Unspecified reactive species

Arthur Choisez,Seiichiro Ishihara,Takuro Ishii,Yidan Xu,Sepideh D Firouzjah,Hisashi Haga,Ryoichi Nagatomi,Joji Kusuyama

Nature communications 15:807 PubMed38280848

2024

Activation of GPR3-β-arrestin2-PKM2 pathway in Kupffer cells stimulates glycolysis and inhibits obesity and liver pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Dong,Guangan Hu,Zhongqi Fan,Huirui Wang,Yinghui Gao,Sisi Wang,Hao Xu,Michael B Yaffe,Matthew G Vander Heiden,Guoyue Lv,Jianzhu Chen

European journal of immunology 53:e2350455 PubMed37471504

2023

Fusobacterium nucleatum infection activates the noncanonical inflammasome and exacerbates inflammatory response in DSS-induced colitis.

Applications

Unspecified application

Species

Unspecified reactive species

Kotchakorn Boonyaleka,Tokuju Okano,Tamako Iida,Anongwee Leewananthawet,Miwa Sasai,Masahiro Yamamoto,Hiroshi Ashida,Toshihiko Suzuki

The European journal of neuroscience 56:6003-6021 PubMed36226387

2022

Impaired spatial navigation and age-dependent hippocampal synaptic dysfunction are associated with chronic inflammatory response in db/db mice.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammed Al-Onaizi,Ahmad Al-Sarraf,Kawthar Braysh,Fatema Kazem,Heba Al-Hussaini,Muddanna Rao,Narayana Kilarkaje,Ayman ElAli

Cell reports 37:110148 PubMed34965424

2021

The microglial P2Y receptor mediates neuronal loss and memory deficits in neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Mar Puigdellívol,Stefan Milde,Anna Vilalta,Tom O J Cockram,David H Allendorf,Jeffrey Y Lee,Jacob M Dundee,Katryna Pampuščenko,Vilmante Borutaite,Hugh N Nuthall,Jack H Brelstaff,Maria Grazia Spillantini,Guy C Brown

Journal of neuroinflammation 18:225 PubMed34635136

2021

Inflammatory neuronal loss in the substantia nigra induced by systemic lipopolysaccharide is prevented by knockout of the P2Y receptor in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Stefan Milde,Francesca W van Tartwijk,Anna Vilalta,Tamara C Hornik,Jacob M Dundee,Mar Puigdellívol,Guy C Brown

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 8:e2100584 PubMed34382372

2021

N2-Polarized Neutrophils Guide Bone Mesenchymal Stem Cell Recruitment and Initiate Bone Regeneration: A Missing Piece of the Bone Regeneration Puzzle.

Applications

Unspecified application

Species

Unspecified reactive species

Bolei Cai,Dan Lin,Yan Li,Le Wang,Jirong Xie,Taiqiang Dai,Fuwei Liu,Mingyue Tang,Lei Tian,Yuan Yuan,Liang Kong,Steve G F Shen

The Journal of reproduction and development 67:257-264 PubMed34176822

2021

Advanced maternal age induces fetal growth restriction through decreased placental inflammatory cytokine expression and immune cell accumulation in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshiki Hirata,Yusuke Katsukura,Yuka Henmi,Ren Ozawa,Sayaka Shimazaki,Akira Kurosawa,Yasushi Torii,Hironori Takahashi,Hisataka Iwata,Takehito Kuwayama,Koumei Shirasuna

Scientific reports 10:9884 PubMed32555372

2020

High fat diet worsens Alzheimer's disease-related behavioral abnormalities and neuropathology in APP/PS1 mice, but not by synergistically decreasing cerebral blood flow.

Applications

Unspecified application

Species

Unspecified reactive species

Oliver Bracko,Lindsay K Vinarcsik,Jean C Cruz Hernández,Nancy E Ruiz-Uribe,Mohammad Haft-Javaherian,Kaja Falkenhain,Egle M Ramanauskaite,Muhammad Ali,Aditi Mohapatra,Madisen A Swallow,Brendah N Njiru,Victorine Muse,Pietro E Michelucci,Nozomi Nishimura,Chris B Schaffer

View all publications

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Cytokine Array - Mouse Cytokine Antibody Array (Membrane, 62 Targets)

Multiplex Protein Detection - Cytokine Array - Mouse Cytokine Antibody Array (Membrane, 62 Targets) (AB133995)

Key facts

Assay type

Reacts with

Target

Applications

Sample types

Reactivity data

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Additional targets

Publications (12)

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Product promise