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ab193659 can be used for the simultaneous detection of 96 Mouse Cytokine proteins in serum, plasma, cell culture media and other liquid samples types.

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Multiplex Protein Detection - Cytokine Array - Mouse Cytokine Antibody Array (Membrane, 96 Targets) (AB193659), expandable thumbnail

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Key facts

Assay type
Semi-quantitative
Reactive species
Mouse
Target
Timp2
(See target data below)

Additional Targets

Shh, Csf2rb, Vegfd, Met, Tyro3, Tnfrsf11b, Tnfrsf1b, Ccl3, Ccl1, Spp1, Il10, Il15, Sell, Cxcl15, Kdr, Cxcl16, Ccl9, Il17a, Lepr, Flt1, Tnf, Timp2, Il2, Tnfrsf19, Mmp9, Igfbp6, Cd40, Tnfrsf18, Ccl12, Ccl2, Tnfsf11, Ccl17, Vegfd, Met, Tyro3, Tnfrsf11b, Tnfrsf1b, Ccl3, Ccl1, Spp1, Il10, Il15, Sell, Cxcl15, Kdr, Cxcl16, Ccl9, Il17a, Lepr, Flt1, Tnf, Timp2, Il2, Tnfrsf19, Mmp9, Igfbp6, Cd40, Tnfrsf18, Ccl12, Ccl2, Tnfsf11, Ccl17, Ccl11, Ccl19, Ccl20, Ccl22, Ccl24, Ccl25, Ccl27, Ccl5, Csf1, Csf3, Cx3cl1, Cxcl1, Cxcl10, Cxcl11, Cxcl12, Cxcl13, Cxcl2, Cxcl5, Cxcl9, Dpp4, Faslg, Fcgr2, Fgf2, Flt3lg, Flt4, Icam1, Ifng, Igf1, Igf2, Igfbp2, Igfbp3, Igfbp5, Il12a, Il12b, Il13, Il17rb, IL1A_MOUSE, Igf2, Il3, Il4, Il5, Il6, Il7, Il9, Kitlg, Lep, Mmp2, Mmp3, Pf4, CXCL7/PBP, Retn, Sele, Selp, Thpo, Timp1, Tnfrsf8, Tnfsf8, Thpo, Tslp, Vcam1, Vegfa, Xcl1, Tnfrsf1a

What's included?

1 x 4 Membranes
Components
1,000X HRP-Conjugated Streptavidin
1 x 50 µL
1X Blocking Buffer
2 x 25 mL
20X Wash Buffer I
1 x 20 mL
20X Wash Buffer II
1 x 20 mL
2X Cell Lysis Buffer
1 x 16 mL
8-Well Incubation Tray (with Lid)
1 x 1 Unit
Biotinylated Antibody Cocktail (C3)
2 x 1 Vial
Biotinylated Antibody Cocktail (C4)
2 x 1 Vial
Detection Buffer C
1 x 2.5 mL
Detection Buffer D
1 x 2.5 mL
Mouse Cytokine Antibody Arrays Membranes (C3)
1 x 4 Unit
Mouse Cytokine Antibody Arrays Membranes (C4)
1 x 4 Unit
Plastic sheets
1 x 1 Unit

Recommended products

ab193659 can be used for the simultaneous detection of 96 Mouse Cytokine proteins in serum, plasma, cell culture media and other liquid samples types.

Key facts

Assay type
Semi-quantitative
Reactive species
Mouse
Target
Timp2
Applications
Multiplex Protein Detection
Sample types
Tissue Lysate, Plasma, Cell culture media, Serum, Other biological fluids, Cell Lysate

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

ab193659 can be used for the simultaneous detection of 96 Mouse Cytokine proteins in serum, plasma, cell culture media and other liquid samples types. It comes as 1 kit including 2 separate membranes labelled C3 and C4 (see below for images showing the array layout on each membrane).

Target names: Axl, Bfgf, BLC (CXCL13), CD30 Ligand (TNFSF8), CD30 (TNFRSF8), CD40 (TNFRSF5), CRG-2, CTACK (CCL27), CXCL16, CD26 (DPPIV), Dtk, Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), E-Selectin, Fas Ligand (TNFSF6), Fc gamma RIIB (CD32b), Flt-3 Ligand, Fractalkine (CX3CL1), GCSF, GITR (TNFRSF18), GM-CSF, HGFR, ICAM-1 (CD54) IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-5, IGFBP-6, IGF-1, IGF-2, IL-1 beta (IL-1 F2), IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-15, IL-17A, IL-17 RB, IL-1 alpha (IL-1 F1), IL-2, IL-3, IL-3 R beta, IL-4, IL-5, IL-6, IL-7, IL-9, I-TAC (CXCL11), KC (CXCL1), Leptin, Leptin R, LIX, L-Selectin (CD62L), Lungkine (CXCL15), Lymphotactin (XCL1), MCP-1 (CCL2), MCP-5, M-CSF, MDC (CCL22), MIG (CXCL9), MIP-1 alpha (CCL3), MIP-1 gamma, MIP-2, MIP-3 beta (CCL19), MIP-3 alpha (CCL20), MMP-2, MMP-3, Osteopontin (SPP1), Osteoprotegerin (TNFRSF11B), Platelet Factor 4 (CXCL4), Pro-MMP-9, P-Selectin, RANTES (CCL5), Resistin, SCF, SDF-1 alpha (CXCL12 alpha), Sonic Hedgehog N-Terminal (Shh-N), TNF RI (TNFRSF1A), TNF RII (TNFRSF1B), TARC (CCL17), I-309 (TCA-3/CCL1), TECK (CCL25), TCK-1 (CXCL7), TIMP-1, TIMP-2, TNF alpha, Thrombopoietin (TPO), TRANCE (TNFSF11), TROY (TNFRSF19), TSLP, VCAM-1 (CD106), VEGF-A, VEGFR1, VEGFR2, VEGFR3, VEGF-D

Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.


**A table listing all of our mouse membrane antibody cytokine arrays and other arrays and the analytes they measure is available .**


Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Multiplex Protein Detection - Cytokine Array - Mouse Cytokine Antibody Array (Membrane, 96 Targets) (ab193659), expandable thumbnail

    Multiplex Protein Detection - Cytokine Array - Mouse Cytokine Antibody Array (Membrane, 96 Targets) (ab193659)

    Typical images obtained with Abcam Cytokine Antibody Arrays.

    Typical images obtained with Abcam Mouse Cytokine Antibody Array - Membrane (96 Targets) ab193659. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at room temperature for 1 minute.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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