JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB133992

Cytokine Array - Rat Cytokine Antibody Array (Membrane, 34 Targets)

Be the first to review this product! Submit a review

|

(11 Publications)

ab133992 is for simultaneous detection of 34 Rat Cytokines.
1 Images
Multiplex Protein Detection - Cytokine Array - Rat Cytokine Antibody Array (Membrane, 34 Targets) (AB133992)
  • Multiplex Protein Detection

Unknown

Multiplex Protein Detection - Cytokine Array - Rat Cytokine Antibody Array (Membrane, 34 Targets) (AB133992)

Typical results obtained with Abcam Human Cytokine Membrane Antibody Array. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at RT for 1 min.

Key facts

Assay type

Semi-quantitative

Reacts with

Rat

Target

Cd86

target

Applications

Multiplex Protein Detection

applications

Sample types

Saliva, Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture media, Cell culture supernatant, Milk, Serum, Other biological fluids, Whole Blood, Cell Lysate

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Multiplex Protein Detection": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Product details

ab133992 is for simultaneous detection of 34 Rat Cytokines. Suitable for all sample types.

Targets: Activin A, Agrin, Thymus Chemokine-1, B7-2/CD86, CINC-1, CINC-2alpha, CINC-3, CNTF, Fas Ligand, Fractalkine, GM-CSF, ICAM-1, IFN-gamma, IL-1 R6, IL-10, IL-13, IL-1alpha, IL-1beta, IL-2, IL-4, IL-6, Leptin, LIX, L-Selectin, MCP-1, MIP-3alpha, MMP-8, beta-NGF, PDGF-AA, Prolactin R, RAGE, TIMP-1, TNF-alpha, VEGF.

Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.

If you are interested in this cytokine array, a table listing all of our rat membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

What's included?

{ "values": { "1x8Membranes": { "sellingSize": "1 x 8 Membranes", "publicAssetCode":"ab133992-1x8Membranes", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plastic sheets", "number":"AB133992-CMP11" }, { "size":"1 x 20 mL", "name":"20X Wash Buffer I", "number":"AB133992-CMP06" }, { "size":"1 x 2.5 mL", "name":"Detection Buffer C", "number":"AB133992-CMP04" }, { "size":"1 x 1 Unit", "name":"8-Well Incubation Tray (with Lid)", "number":"AB133992-CMP09" }, { "size":"4 x 1 Vial", "name":"Biotin-Conjugated Anti-Cytokines", "number":"AB133992-CMP02" }, { "size":"1 x 20 mL", "name":"20X Wash Buffer II", "number":"AB133992-CMP07" }, { "size":"1 x 50 µL", "name":"1,000X HRP-Conjugated Streptavidin", "number":"AB133992-CMP03" }, { "size":"1 x 16 mL", "name":"2X Cell Lysis Buffer", "number":"AB133992-CMP08" }, { "size":"2 x 25 mL", "name":"1X Blocking Buffer", "number":"AB133992-CMP01" }, { "size":"1 x 2.5 mL", "name":"Detection Buffer D", "number":"AB133992-CMP05" }, { "size":"2 x 4 Unit", "name":"Cytokine Antibody Array Membranes", "number":"AB133992-CMP10" } ] }, "1x4Membranes": { "sellingSize": "1 x 4 Membranes", "publicAssetCode":"ab133992-1x4Membranes", "assetComponentDetails": [ { "size":"1 x 1.5 mL", "name":"Detection Buffer C", "number":"AB133992-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"20X Wash Buffer I", "number":"AB133992-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"20X Wash Buffer II", "number":"AB133992-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"8-Well Incubation Tray (with Lid)", "number":"AB133992-CMP09", "productcode":"" }, { "size":"1 x 25 mL", "name":"1X Blocking Buffer", "number":"AB133992-CMP01", "productcode":"" }, { "size":"1 x 50 µL", "name":"1,000X HRP-Conjugated Streptavidin", "number":"AB133992-CMP03", "productcode":"" }, { "size":"1 x 4 Unit", "name":"Cytokine Antibody Array Membranes", "number":"AB133992-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotin-Conjugated Anti-Cytokines", "number":"AB133992-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"2X Cell Lysis Buffer", "number":"AB133992-CMP08", "productcode":"" }, { "size":"1 x 1.5 mL", "name":"Detection Buffer D", "number":"AB133992-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plastic sheets", "number":"AB133992-CMP11", "productcode":"" } ] } } }

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Product protocols

Target data

See full target information Cd86

Additional targets

Faslg,Sell,Mmp8,Il1rl2

Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Cells 13: PubMed39056770

2024

Octadecaneuropeptide, ODN, Promotes Cell Survival against 6-OHDA-Induced Oxidative Stress and Apoptosis by Modulating the Expression of miR-34b, miR-29a, and miR-21in Cultured Astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Amine Bourzam,Yosra Hamdi,Seyma Bahdoudi,Karthi Duraisamy,Mouna El Mehdi,Magali Basille-Dugay,Omayma Dlimi,Maher Kharrat,Anne Vejux,Gérard Lizard,Taoufik Ghrairi,Benjamin Lefranc,David Vaudry,Jean A Boutin,Jérôme Leprince,Olfa Masmoudi-Kouki

International journal of molecular sciences 25: PubMed39000527

2024

Nematode Galectin Inhibits Basophilic Leukaemia RBL-2H3 Cells Apoptosis in IgE-Mediated Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Marta Maruszewska-Cheruiyot,Ludmiła Szewczak,Katarzyna Krawczak-Wójcik,Michael James Stear,Katarzyna Donskow-Łysoniewska

Bioengineering & translational medicine 9:e10635 PubMed38435829

2024

Schwann cells acquire a repair phenotype after assembling into spheroids and show enhanced in vivo therapeutic potential for promoting peripheral nerve repair.

Applications

Unspecified application

Species

Unspecified reactive species

Shih-Heng Chen,Hsin-Wen Wang,Pei-Ching Yang,Shih-Shien Chen,Chia-Hsin Ho,Pei-Ching Yang,Ying-Chi Kao,Shao-Wen Liu,Han Chiu,Yu-Jie Lin,Er-Yuan Chuang,Jen-Huang Huang,Huang-Kai Kao,Chieh-Cheng Huang

Scientific reports 13:8622 PubMed37244975

2023

VEGF overexpressed mesoangioblasts enhance urethral and vaginal recovery following simulated vaginal birth in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Marina G M C Mori da Cunha,Bernard K van der Veer,Giorgia Giacomazzi,Katerina Mackova,Laura Cattani,Kian Peng Koh,Greetje Vande Velde,Rik Gijsbers,Maarten Albersen,Maurilio Sampaolesi,Jan Deprest

JCI insight 7: PubMed36134661

2022

Loss of TRPV2-mediated blood flow autoregulation recapitulates diabetic retinopathy in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Michael O'Hare,Gema Esquiva,Mary K McGahon,Jose Manuel Romero Hombrebueno,Josy Augustine,Paul Canning,Kevin S Edgar,Peter Barabas,Thomas Friedel,Patrizia Cincolà,Jennifer Henry,Katie Mayne,Hannah Ferrin,Alan W Stitt,Timothy J Lyons,Derek P Brazil,David J Grieve,J Graham McGeown,Tim M Curtis

Biomedicines 10: PubMed35453528

2022

Anti-Inflammatory CDGSH Iron-Sulfur Domain 2: A Biomarker of Central Nervous System Insult in Cellular, Animal Models and Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Woon-Man Kung,Chai-Ching Lin,Wei-Jung Chen,Li-Lin Jiang,Yu-Yo Sun,Kuang-Hui Hsieh,Muh-Shi Lin

Purinergic signalling 18:135-154 PubMed34964926

2021

P2X7 receptor: the regulator of glioma tumor development and survival.

Applications

Unspecified application

Species

Unspecified reactive species

Damian Matyśniak,Vira Chumak,Natalia Nowak,Artur Kukla,Lilya Lehka,Magdalena Oslislok,Paweł Pomorski

Experimental biology and medicine (Maywood, N.J.) 246:695-706 PubMed33148012

2020

Prenatal indole-3-carbinol administration activates aryl hydrocarbon receptor-responsive genes and attenuates lung injury in a bronchopulmonary dysplasia model.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriela Guzmán-Navarro,Mario Bermúdez de León,Irene Martín-Estal,Raquel Cuevas-Díaz Durán,Laura Villarreal-Alvarado,Anakaren Vaquera-Vázquez,Tania Cuevas-Cerda,Karina Garza-García,Luis Eduardo Cuervo-Pérez,Álvaro Barbosa-Quintana,José Eduardo Pérez-Saucedo,Víctor J Lara-Díaz,Fabiola Castorena-Torres

PloS one 14:e0215678 PubMed31009502

2019

Cytokines secreted from bone marrow derived mesenchymal stem cells promote apoptosis and change cell cycle distribution of K562 cell line as clinical agent in cell transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Ezzatollah Fathi,Raheleh Farahzadi,Behnaz Valipour,Zohreh Sanaat

Frontiers in microbiology 9:3292 PubMed30687277

2019

Fermentation Products of sp. nov. BD3526 Alleviates the Symptoms of Type 2 Diabetes Mellitus in GK Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenyi Qiao,Jin Han,Huafeng Feng,Huajun Zheng,Jiang Wu,Caixia Gao,Meng Yang,Chunping You,Zhenmin Liu,Zhengjun Wu
View all publications
websiteProtocolBooklet
en

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com