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ab133992 is for simultaneous detection of 34 Rat Cytokines.

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Multiplex Protein Detection - Cytokine Array - Rat Cytokine Antibody Array (Membrane, 34 Targets) (AB133992), expandable thumbnail

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Key facts

Assay type
Semi-quantitative
Reactive species
Rat
Target
Cd86
(See target data below)

Target data

Additional Targets

Faslg, Sell, Mmp8, Il1rl2

What's included?

1 x 8 Membranes
Components
1,000X HRP-Conjugated Streptavidin
1 x 50 µL
1X Blocking Buffer
2 x 25 mL
20X Wash Buffer I
1 x 20 mL
20X Wash Buffer II
1 x 20 mL
2X Cell Lysis Buffer
1 x 16 mL
8-Well Incubation Tray (with Lid)
1 x 1 Unit
Biotin-Conjugated Anti-Cytokines
4 x 1 Vial
Cytokine Antibody Array Membranes
2 x 4 Unit
Detection Buffer C
1 x 2.5 mL
Detection Buffer D
1 x 2.5 mL
Plastic sheets
1 x 1 Unit

Recommended products

ab133992 is for simultaneous detection of 34 Rat Cytokines.

Key facts

Assay type
Semi-quantitative
Reactive species
Rat
Target
Cd86
Applications
Multiplex Protein Detection
Sample types
Saliva, Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture media, Cell culture supernatant, Milk, Serum, Other biological fluids, Whole Blood, Cell Lysate

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

ab133992 is for simultaneous detection of 34 Rat Cytokines. Suitable for all sample types.

Targets: Activin A, Agrin, Thymus Chemokine-1, B7-2/CD86, CINC-1, CINC-2alpha, CINC-3, CNTF, Fas Ligand, Fractalkine, GM-CSF, ICAM-1, IFN-gamma, IL-1 R6, IL-10, IL-13, IL-1alpha, IL-1beta, IL-2, IL-4, IL-6, Leptin, LIX, L-Selectin, MCP-1, MIP-3alpha, MMP-8, beta-NGF, PDGF-AA, Prolactin R, RAGE, TIMP-1, TNF-alpha, VEGF.

Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.


If you are interested in this cytokine array, a table listing all of our rat membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.


Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Multiplex Protein Detection - Cytokine Array - Rat Cytokine Antibody Array (Membrane, 34 Targets) (ab133992), expandable thumbnail

    Multiplex Protein Detection - Cytokine Array - Rat Cytokine Antibody Array (Membrane, 34 Targets) (ab133992)

    Typical results obtained with Abcam Human Cytokine Membrane Antibody Array. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at RT for 1 min.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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