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AB197414

Feline Cytokine Antibody Array (10 Targets) - Quantitative

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ab197414 can be used for quantitative measurement of 10 Feline cytokines.

View Alternative Names

Interferon gamma, IFN-gamma, IFNG, Interleukin-5, IL-5, Eosinophil differentiation factor, T-cell replacing factor, TRF, IL5, IL8, CXCL8, Interleukin-8, IL-8, C-X-C motif chemokine 8, Chemokine (C-X-C motif) ligand 8, Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10, Interleukin-12 subunit beta, IL-12B, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p40, CLMF p40, IL12B, Interleukin-1 beta, IL-1 beta, IL1B, Interleukin-4, IL-4, B-cell stimulatory factor 1, Lymphocyte stimulatory factor 1, BSF-1, IL4, CD178, FASL, TNFSF6, FASLG, Tumor necrosis factor ligand superfamily member 6, Fas antigen ligand, Fas ligand, FasL

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Multiplex Protein Detection - Feline Cytokine Antibody Array (10 Targets) - Quantitative (AB197414)
  • Multiplex Protein Detection

Supplier Data

Multiplex Protein Detection - Feline Cytokine Antibody Array (10 Targets) - Quantitative (AB197414)

Standard Curve obtained with Abcam Feline Cytokine Antibody Array - Quantitative (10 targets) (ab197414).

Multiplex Protein Detection - Feline Cytokine Antibody Array (10 Targets) - Quantitative (AB197414)
  • Multiplex Protein Detection

Supplier Data

Multiplex Protein Detection - Feline Cytokine Antibody Array (10 Targets) - Quantitative (AB197414)

Array Map for Feline Cytokine Antibody Array - Quantitative (10 targets) (ab197414).

Multiplex Protein Detection - Feline Cytokine Antibody Array (10 Targets) - Quantitative (AB197414)
  • Multiplex Protein Detection

Supplier Data

Multiplex Protein Detection - Feline Cytokine Antibody Array (10 Targets) - Quantitative (AB197414)

Assay Summary for Feline Cytokine Antibody Array - Quantitative (10 targets) (ab197414).

Key facts

Reacts with

Cat

Target

IFNG

target

Detection method

Fluorescent

Assay Platform

Multiplex

Sample types

Tissue Lysate, Plasma, Cell culture media, Serum, Other biological fluids, Cell Lysate

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Product details

ab197414 can be used for quantitative measurement of 10 Feline cytokines. Suitable for serum, plasma, cell culture media, other body fluids, cell and tissue lysates.
Targets names: Fas, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-8, IL-10, IL-12p40 and RANTES.

Quantitative antibody arrays can be used to quantitate up to 40 cytokines with as little as 50 µL of sample. Arrays are available for 400 human and 200 mouse proteins. Each glass slide is spotted with 16 identical antibody arrays and is provided with a 16 well gasket to allow separate samples to be applied to each array. Each antibody is spotted in quadruplicate in each array. For reproducible quantitation, eight of the arrays are used with a cocktail of protein standards to produce a standard curve. The same 8 standard curve arrays can be used across multiple slides, allowing measurement of 22 experimental samples with 2 slides, 36 with 3 slides etc. For high through-put, 4 slides can be nested into a tray matching a standard microplate, allowing automated processing with a liquid handling workstation. Array processing can be completed within one working day (see workflow diagram below) and arrays can be analyzed with a wide number of laser glass slide array / gene microarray scanners. If you don’t have a suitable scanner then we recommend our membrane antibody arrays, which can be analyzed with any WB chemiluminescent reader. "

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL (By similarity). Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation (By similarity).
See full target information IFNG

Additional targets

CXCL8,FASLG,IL10,IL12B,IL1B,IL5,IL4
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Product promise

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