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For the simultaneous detection of 20 Human Cytokine levels in 4 or 8 samples.

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Images

Multiplex Protein Detection - Human Acute Kidney Injury Antibody Array - Membrane (20 targets) (AB169806), expandable thumbnail

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Key facts

Assay type

Semi-quantitative

Reactive species

Human

Target
ALB
(See target data below)

Target data

Function

Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs (Probable). Its main function is the regulation of the colloidal osmotic pressure of blood (Probable). Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (PubMed:19021548). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed:6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed:6234017).

Targets

B2M, CALB1, CCL2, CLU, CST3, CXCL10, CXCL16, FABP1, GPNMB, HAVCR1, HGF, LCN2, MIF, SPP1, TFF3, TIMP1, TNFRSF1A, VCAM1, VEGFA

Alternative names

What's included?

1 x 8 Membranes
Components
1,000X HRP-Conjugated Streptavidin
1 x 50 µL
1X Blocking Buffer
2 x 25 mL
2000X Biotin-Conjugated Anti-Cytokines
4 x 1 Vial
20X Wash Buffer I
1 x 20 mL
20X Wash Buffer II
1 x 20 mL
2X Cell Lysis Buffer
1 x 16 mL
8-Well Incubation Tray (with Lid)
1 x 1 Unit
Detection Buffer C
1 x 2.5 mL
Detection Buffer D
1 x 2.5 mL
Human Acute Kidney Injury Antibody Array Membranes
2 x 4 Unit
Plastic sheets
1 x 1 Unit

Recommended products

For the simultaneous detection of 20 Human Cytokine levels in 4 or 8 samples.

Key facts

Assay type

Semi-quantitative

Reactive species

Human

Target

ALB

Applications

Multiplex Protein Detection

Sample types

Saliva, Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture media, Cell culture supernatant, Milk, Serum, Other biological fluids, Whole Blood, Cell Lysate

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

For the simultaneous detection of 20 Human Cytokine levels in 4 or 8 samples. Suitable for all sample types.

Targets:

KIM-1, Albumin (ALB), Osteopontin (Osteopontin), Trefoil Factor 3 (TFF3), B2M, Clusterin (CLU), CXCL16, Osteoactivin, Liver-type fatty acid binding protein (L-FABP), MCP-1, sTNFRI, Calbindin (CALB1), Interferon gamma-induced protein 10 (IP-10), Cystatin C, HGF, MIF, NGAL, TIMP-1, VCAM-1, VEGF-A

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Product promise

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1 product image

  • Multiplex Protein Detection - Human Acute Kidney Injury Antibody Array - Membrane (20 targets) (ab169806), expandable thumbnail

    Multiplex Protein Detection - Human Acute Kidney Injury Antibody Array - Membrane (20 targets) (ab169806)

    Typical results obtained with Abcam Membrane Antibody Arrays. Image above presents typical images obtained with Abcam Human Cytokine Membrane Antibody Array. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at RT for 1 min.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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