ab197434 can be used for quantitative measurement of 17 Human Adhesion Molecule.
Images
Key facts
- Assay type
- Quantitative
- Reactive species
- Human
- Target
- ALCAM
(See target data below) - Assay Platform
- Multiplex
Target data
Function
Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts (PubMed:15048703, PubMed:15496415, PubMed:16352806, PubMed:23169771, PubMed:24945728, PubMed:7760007). Promotes T-cell activation and proliferation via its interactions with CD6 (PubMed:15048703, PubMed:16352806, PubMed:24945728). Contributes to the formation and maturation of the immunological synapse via its interactions with CD6 (PubMed:15294938, PubMed:16352806). Mediates homotypic interactions with cells that express ALCAM (PubMed:15496415, PubMed:16352806). Acts as a ligand for the LILRB4 receptor, enhancing LILRB4-mediated inhibition of T cell proliferation (PubMed:29263213). Required for normal hematopoietic stem cell engraftment in the bone marrow (PubMed:24740813). Mediates attachment of dendritic cells onto endothelial cells via homotypic interaction (PubMed:23169771). Inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions (PubMed:15496415, PubMed:23169771). Required for normal organization of the lymph vessel network. Required for normal hematopoietic stem cell engraftment in the bone marrow. Plays a role in hematopoiesis; required for normal numbers of hematopoietic stem cells in bone marrow. Promotes in vitro osteoblast proliferation and differentiation (By similarity). Promotes neurite extension, axon growth and axon guidance; axons grow preferentially on surfaces that contain ALCAM. Mediates outgrowth and pathfinding for retinal ganglion cell axons (By similarity). Isoform 3. Inhibits activities of membrane-bound isoforms by competing for the same interaction partners. Inhibits cell attachment via homotypic interactions. Promotes endothelial cell migration. Inhibits endothelial cell tube formation.
Additional Targets
BCAM, CDH1, CDH3, CDH5, CEACAM1, EPCAM, ICAM1, ICAM2, ICAM3, NCAM1, NRCAM, PECAM1, SELE, SELL, SELP, VCAM1
Alternative names
CD166, MEMD, ALCAM, CD166 antigen, Activated leukocyte cell adhesion molecule
What's included?
Recommended products
ab197434 can be used for quantitative measurement of 17 Human Adhesion Molecule.
Key facts
- Assay type
- Quantitative
- Reactive species
- Human
- Target
- ALCAM
- Detection method
- Fluorescent
- Assay Platform
- Multiplex
- Sample types
- Tissue Lysate, Plasma, Cell culture media, Serum, Other biological fluids, Cell Lysate
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
ab197434 can be used for quantitative measurement of 17 Human Adhesion Molecule. Suitable for serum, plasma, cell culture media, other body fluids, cell and tissue lysates.
Target names: ALCAM, BCAM, CEACAM-1, E-Cadherin, EpCAM,E-Selectin, ICAM-1, ICAM-2, ICAM-3, L-Selectin, NCAM-1, NrCAM, P-Cadherin, P-Selectin, PECAM-1, VCAM-1, VE-Cadherin.
The analytes measured by this kit are split across a single array on the same slide, provided in the kit.
Quantitative antibody arrays can be used to quantitate up to 40 cytokines with as little as 50 µL of sample. Arrays are available for 400 human and 200 mouse proteins. Each glass slide is spotted with 16 identical antibody arrays and is provided with a 16 well gasket to allow separate samples to be applied to each array.Each antibody is spotted in quadruplicate on each array. For reproducible quantitation, eight of the arrays are used with a cocktail of protein standards to produce a standard curve. The same 8 standard curve arrays can be used across multiple slides, allowing measurement of 22 experimental samples with 2 slides, 36 with 3 slides etc. For high through-put, 4 slides can be nested into a tray matching a standard microplate (not supplied), allowing automated processing with a liquid handling workstation. Array processing can be completed within one working day and arrays can be analyzed with a wide number of laser glass slide array / gene microarray scanners (see scanner requirements at ). If you don’t have a suitable scanner then we recommend our membrane antibody arrays, which can be analyzed with any WB chemiluminescent reader. See below for a schematic protocol, the fluorophore is a dye with equivalent to excitation/emission to Cy3.
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3 product images
Multiplex Protein Detection - Human Adhesion Molecule Antibody Array (17 Targets) - Quantitative (ab197434)
Assay summary for Abcam Human Adhesion Molecule Antibody Array (17 Targets) - Quantitative (ab197434).
Multiplex Protein Detection - Human Adhesion Molecule Antibody Array (17 Targets) - Quantitative (ab197434)
Array map for Abcam Human Adhesion Molecule Antibody Array (17 Targets) - Quantitative (ab197434).
Multiplex Protein Detection - Human Adhesion Molecule Antibody Array (17 Targets) - Quantitative (ab197434)
Standard Curve obtained using Abcam Human Adhesion Molecule Antibody Array (10 Targets) - Quantitative (ab197434).
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