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ab197418 can be used for quantitative measurement of 60 Human angiogenesis markers.

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Multiplex Protein Detection - Human Angiogenesis Antibody Array (60 Targets) - Quantitative (AB197418), expandable thumbnail

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Key facts

Assay type

Quantitative

Reactive species

Human

Target
Inhibin beta A chain
(See target data below)
Assay Platform

Multiplex

Target data

Function

Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.

Targets

AGRP, ANG, ANGPT1, ANGPT2, PLG, ANGPTL4, FGF2, CXCL16, CXCL5, EGF, FGF4, FST, CSF3, CSF2, CXCL3, HBEGF, HGF, CCL1, IFNG, IGF1, IL1A, IL1B, IL10, IL12B, IL12A, IL17A, IL2, IL4, IL6, CXCL8, CXCL10, CXCL11, LEP, LIF, CCL2, CCL8, CCL7, CCL13, MMP1, PDGFB, CD31, PGF, CCL5, Protransforming growth factor alpha, TGFB1, TGFB3, THPO, TIE1, TEK, TIMP1, TIMP2, TNF, LTA, PLAUR, VEGFA, VEGFD, KDR, FLT4

Alternative names

What's included?

50 Test
Components
20X Wash Buffer I
12 x 30 mL
20X Wash Buffer II
4 x 30 mL
Adhesive Film
8 x 1 Unit
Cy3 equivalent dye-conjugated Streptavidin
8 x 5 µL
Human Angiogenesis Array A (30 T) Biotinylated Antibody Cocktail
4 x 25 µL
Human Angiogenesis Array A (30 T) Glass Slide
4 x 1 Slide
Human Angiogenesis Array A (30 T) Lyophilized Cytokine Standard Mix
2 x 1 Vial
Human Angiogenesis Array B (30 T) Biotinylated Antibody Cocktail
4 x 25 µL
Human Angiogenesis Array B (30 T) Glass Slide
4 x 1 Slide
Human Angiogenesis Array B (30 T) Lyophilized Cytokine Standard Mix
2 x 1 Vial
Sample Diluent
4 x 15 mL
Slide Washer/Dryer (30 mL Centrifuge Tube)
4 x 1 Unit

Recommended products

ab197418 can be used for quantitative measurement of 60 Human angiogenesis markers.

Key facts

Assay type

Quantitative

Reactive species

Human

Target

Inhibin beta A chain

Detection method

Fluorescent

Assay Platform

Multiplex

Sample types

Tissue Lysate, Plasma, Cell culture media, Serum, Other biological fluids, Cell Lysate

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

ab197418 can be used for quantitative measurement of 60 Human angiogenesis markers. Suitable for serum, plasma, cell culture media, other body fluids, cell and tissue lysates.Targets names: Activin A, AgRP, Angiogenin, ANG-2, ANGPTL4, bFGF, ENA-78, GRO, HB-EGF, HGF, IFNγ, IGF-I, IL-1α, IL-2, IL-6, IL-8, IL-17, IP-10, Leptin, LIF, MCP-1, PDGF-BB, PIGF, RANTES, TGFβ1, TIMP-1, TIMP-2, TNFα, TNFβ, TPO, ANG-1, Angiostatin, CXCL16, EGF, FGF-4, Follistatin, G-CSF, GM-CSF, I-309, IL-1β, IL-4, IL-10, IL-12p40, IL-12p70, I-TAC, MCP-2, MCP-3, MCP-4, MMP-1, MMP-9, PECAM-1, TGFα, TGFβ3, Tie-1, Tie-2, uPAR, VEGF, VEGF R2, VEGF R3, VEGF-D.

The analytes measured by this kit are split across 2 different arrays on different slides, both of which are provided in the kit.

Quantitative antibody arrays can be used to quantitate up to 40 cytokines with as little as 50 µL of sample. Arrays are available for 400 human and 200 mouse proteins. Each glass slide is spotted with 16 identical antibody arrays and is provided with a 16 well gasket to allow separate samples to be applied to each array. Each antibody is spotted in quadruplicate in each array. For reproducible quantitation, eight of the arrays are used with a cocktail of protein standards to produce a standard curve. The same 8 standard curve arrays can be used across multiple slides, allowing measurement of 22 experimental samples with 2 slides, 36 with 3 slides etc. For high through-put, 4 slides can be nested into a tray matching a standard microplate, allowing automated processing with a liquid handling workstation. Array processing can be completed within one working day (see workflow diagram below) and arrays can be analyzed with a wide number of laser glass slide array / gene microarray scanners. If you don’t have a suitable scanner then we recommend our membrane antibody arrays, which can be analyzed with any WB chemiluminescent reader. The standard is affinity purified. "

Product promise

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1 product image

  • Multiplex Protein Detection - Human Angiogenesis Antibody Array (60 Targets) - Quantitative (ab197418), expandable thumbnail

    Multiplex Protein Detection - Human Angiogenesis Antibody Array (60 Targets) - Quantitative (ab197418)

    Assay Summary for Human Angiogenesis Antibody Array A (30 Targets) - Quantitative (ab197418).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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