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AB134000

Human Angiogenesis Antibody Array - Membrane (20 Targets)

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(9 Publications)

ab13400 is for simultaneous detection of 20 Human Angiogenic factors.

View Alternative Names

RNASE5, ANG, Angiogenin, Ribonuclease 5, RNase 5, MCP1, SCYA2, CCL2, C-C motif chemokine 2, HC11, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, Small-inducible cytokine A2, MCAF, MCP-1, IFNB2, IL6, Interleukin-6, IL-6, B-cell stimulatory factor 2, CTL differentiation factor, Hybridoma growth factor, Interferon beta-2, BSF-2, CDF, IFN-beta-2, FGFB, FGF2, Fibroblast growth factor 2, FGF-2, Basic fibroblast growth factor, Heparin-binding growth factor 2, bFGF, HBGF-2, PDGF2, SIS, PDGFB, Platelet-derived growth factor subunit B, PDGF subunit B, PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis, ENA78, SCYB5, CXCL5, C-X-C motif chemokine 5, ENA-78(1-78), Epithelial-derived neutrophil-activating protein 78, Neutrophil-activating peptide ENA-78, Small-inducible cytokine B5, D17S136E, SCYA5, CCL5, C-C motif chemokine 5, EoCP, Eosinophil chemotactic cytokine, SIS-delta, Small-inducible cytokine A5, T cell-specific protein P228, T-cell-specific protein RANTES, TCP228, TGFB, TGFB1, Transforming growth factor beta-1 proprotein, Metalloproteinase inhibitor 2, CSC-21K, Tissue inhibitor of metalloproteinases 2, TIMP-2, TIMP2, Pro-epidermal growth factor, EGF, GRO3, GROG, SCYB3, CXCL3, C-X-C motif chemokine 3, GRO-gamma(1-73), Growth-regulated protein gamma, Macrophage inflammatory protein 2-beta, GRO-gamma, MIP2-beta, Interferon gamma, IFN-gamma, Immune interferon, IFNG, IBP1, IGF-1, IGF1, Insulin-like growth factor 1, Insulin-like growth factor I, Mechano growth factor, Somatomedin-C, IGF-I, MGF, IL8, CXCL8, Interleukin-8, IL-8, C-X-C motif chemokine 8, Chemokine (C-X-C motif) ligand 8, Emoctakin, Granulocyte chemotactic protein 1, Monocyte-derived neutrophil chemotactic factor, Monocyte-derived neutrophil-activating peptide, Neutrophil-activating protein 1, Protein 3-10C, T-cell chemotactic factor, GCP-1, MDNCF, MONAP, NAP-1, OB, OBS, LEP, Leptin, Obese protein, Obesity factor, PGFL, PLGF, PGF, Placenta growth factor, PlGF, MGDF, THPO, Thrombopoietin, C-mpl ligand, Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, Myeloproliferative leukemia virus oncogene ligand, ML, CLGI, TIMP, TIMP1, Metalloproteinase inhibitor 1, Erythroid-potentiating activity, Fibroblast collagenase inhibitor, Tissue inhibitor of metalloproteinases 1, EPA, Collagenase inhibitor, TIMP-1, VEGF, VEGFA, L-VEGF, Vascular permeability factor, VPF, FIGF, VEGFD, Vascular endothelial growth factor D, VEGF-D, c-Fos-induced growth factor

3 Images
ELISA - Human Angiogenesis Antibody Array - Membrane (20 Targets) (AB134000)
  • ELISA

Unknown

ELISA - Human Angiogenesis Antibody Array - Membrane (20 Targets) (AB134000)

THP-1 cells (Human peripheral blood monocytes) were seeded at 1x106 cells/mL and cultured in RPMI media supplemented with 10% fetal calf serum, 0.05mM 2-mercaptoethanol, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were cultured for 2 days in the presence of LPS. Conditioned media was harvested after 48 hours post-stimulation, aliquoted and assayed using ab134000. Mean pixel density was quantified using CCD camera software analysis.

HUVEC cells (Human umbilical vein endothelial cells) were seeded at 1x106 cells/mL and cultured in RPMI media supplemented with 10% fetal calf serum, 0.1 mg/mL heparin, 0.05 mg/mL ECGS, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab134000. Mean pixel density was quantified using CCD camera software analysis.

ELISA - Human Angiogenesis Antibody Array - Membrane (20 Targets) (AB134000)
  • ELISA

Unknown

ELISA - Human Angiogenesis Antibody Array - Membrane (20 Targets) (AB134000)

Human serum and plasma (EDTA) from a pooled donor (n=50) sample was diluted to 25% and assayed using ab134000. Mean pixel density was quantified using CCD camera software analysis.

Multiplex Protein Detection - Human Angiogenesis Antibody Array - Membrane (20 Targets) (AB134000)
  • Multiplex Protein Detection

Unknown

Multiplex Protein Detection - Human Angiogenesis Antibody Array - Membrane (20 Targets) (AB134000)

Typical results obtained with Abcam Human Cytokine Antibody Array - Membrane. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at RT for 1 min.

Key facts

Reacts with

Human

Target

ANG

target

Applications

Multiplex Protein Detection

applications

Detection method

Luminescent

Sample types

Saliva, Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture media, Cell culture supernatant, Milk, Serum, Other biological fluids, Whole Blood, Cell Lysate

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Complementary Products

Reagents, Controls & Accessories

AB270055

Protease Inhibitor Cocktail EDTA Free (100X)

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Multiplex Kits

AB193655

Human Angiogenesis Antibody Array - Membrane (43 Targets)

Multiplex Protein Detection - Human Angiogenesis Antibody Array - Membrane (43 Targets) (AB193655)

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Multiplex Kits

AB133998

Cytokine Array - Human Cytokine Antibody Array (Membrane, 80 Targets)

Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 80 Targets) (AB133998)

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Multiplex Kits

AB133997

Cytokine Array - Human Cytokine Antibody Array (Membrane, 42 Targets)

Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 42 Targets) (AB133997)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Multiplex Protein Detection": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab13400 is for simultaneous detection of 20 Human Angiogenic factors. Suitable for all sample types.

Targets: Angiogenin, EGF, ENA-78, bFGF, GRO, IFN-gamma, IGF-I, IL-6, IL-8, Leptin, MCP-1, PDGF-BB, PIGF, RANTES, TGF-beta1, TIMP-1, TIMP-2, Thrombopoietin, VEGF-A, VEGF-D

Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.

If you are interested in this cytokine array, arrays ab133997, ab133998 and ab169819 may also be of interest.

A table listing all of our human membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "1x4Membranes": { "sellingSize": "1 x 4 Membranes", "publicAssetCode":"ab134000-1x4Membranes", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"8-Well Incubation Tray (with Lid)", "number":"AB134000-CMP10" }, { "size":"1 x 25 mL", "name":"1X Blocking Buffer", "number":"AB134000-CMP03" }, { "size":"2 x 1 Vial", "name":"Biotinylated Antibody Cocktail (C1)", "number":"AB134000-CMP01" }, { "size":"1 x 50 µL", "name":"1,000X HRP-Conjugated Streptavidin", "number":"AB134000-CMP04" }, { "size":"1 x 4 Unit", "name":"Human Angiogenesis Antibody Array Membranes (C1)", "number":"AB134000-CMP02" }, { "size":"1 x 1.5 mL", "name":"Detection Buffer C", "number":"AB134000-CMP05" }, { "size":"1 x 10 mL", "name":"2X Cell Lysis Buffer", "number":"AB134000-CMP09" }, { "size":"1 x 10 mL", "name":"20X Wash Buffer II", "number":"AB134000-CMP08" }, { "size":"1 x 1.5 mL", "name":"Detection Buffer D", "number":"AB134000-CMP06" }, { "size":"1 x 10 mL", "name":"20X Wash Buffer I", "number":"AB134000-CMP07" }, { "size":"1 x 1 Unit", "name":"Plastic sheets", "number":"AB134000-CMP11" } ] }, "1x8Membranes": { "sellingSize": "1 x 8 Membranes", "publicAssetCode":"ab134000-1x8Membranes", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plastic sheets", "number":"AB134000-CMP11", "productcode":"" }, { "size":"2 x 25 mL", "name":"1X Blocking Buffer", "number":"AB134000-CMP03", "productcode":"" }, { "size":"2 x 4 Unit", "name":"Human Angiogenesis Antibody Array Membranes (C1)", "number":"AB134000-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"8-Well Incubation Tray (with Lid)", "number":"AB134000-CMP10", "productcode":"" }, { "size":"1 x 50 µL", "name":"1,000X HRP-Conjugated Streptavidin", "number":"AB134000-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"20X Wash Buffer II", "number":"AB134000-CMP08", "productcode":"" }, { "size":"4 x 1 Vial", "name":"Biotinylated Antibody Cocktail (C1)", "number":"AB134000-CMP01", "productcode":"" }, { "size":"1 x 16 mL", "name":"2X Cell Lysis Buffer", "number":"AB134000-CMP09", "productcode":"" }, { "size":"1 x 2.5 mL", "name":"Detection Buffer C", "number":"AB134000-CMP05", "productcode":"" }, { "size":"1 x 2.5 mL", "name":"Detection Buffer D", "number":"AB134000-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"20X Wash Buffer I", "number":"AB134000-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Secreted ribonuclease that can either promote or restrict cell proliferation of target cells, depending on the context (PubMed : 12051708, PubMed : 1400510, PubMed : 19332886, PubMed : 20129916, PubMed : 21855800, PubMed : 23047679, PubMed : 23843625, PubMed : 2424496, PubMed : 2459697, PubMed : 2730651, PubMed : 27518564, PubMed : 28176817, PubMed : 29100074, PubMed : 29748193, PubMed : 3122207, PubMed : 32510170, PubMed : 38718836, PubMed : 8159680, PubMed : 8570639, PubMed : 8622921, PubMed : 9578571). Endocytosed in target cells via its receptor PLXNB2 and translocates to the cytoplasm or nucleus (PubMed : 29100074, PubMed : 32510170). Under stress conditions, localizes to the cytoplasm and promotes the assembly of stress granules (SGs) : specifically cleaves a subset of tRNAs within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs), resulting in translation repression and inhibition of cell proliferation (PubMed : 1400510, PubMed : 19332886, PubMed : 20129916, PubMed : 21855800, PubMed : 23047679, PubMed : 27518564, PubMed : 29100074, PubMed : 29748193, PubMed : 32510170, PubMed : 38718836). tiRNas also prevent formation of apoptosome, thereby promoting cell survival (By similarity). Preferentially cleaves RNAs between a pyrimidine and an adenosine residue, suggesting that it cleaves the anticodon loop of tRNA(Ala) (32-UUAGCAU-38) after positions 33 and 36 (PubMed : 3289612, PubMed : 38718836). Cleaves a subset of tRNAs, including tRNA(Ala), tRNA(Glu), tRNA(Gly), tRNA(Lys), tRNA(Val), tRNA(His), tRNA(Asp) and tRNA(Sec) (PubMed : 31582561). Under growth conditions and in differentiated cells, translocates to the nucleus and stimulates ribosomal RNA (rRNA) transcription, including that containing the initiation site sequences of 45S rRNA, thereby promoting cell growth and proliferation (PubMed : 12051708, PubMed : 15735021, PubMed : 27518564, PubMed : 29100074, PubMed : 8127865). Angiogenin induces vascularization of normal and malignant tissues via its ability to promote rRNA transcription (PubMed : 19354288, PubMed : 4074709, PubMed : 8448182). Involved in hematopoietic stem and progenitor cell (HSPC) growth and survival by promoting rRNA transcription in growth conditions and inhibiting translation in response to stress, respectively (PubMed : 27518564). Mediates the crosstalk between myeloid and intestinal epithelial cells to protect the intestinal epithelial barrier integrity : secreted by myeloid cells and promotes intestinal epithelial cells proliferation and survival (PubMed : 32510170). Also mediates osteoclast-endothelial cell crosstalk in growing bone : produced by osteoclasts and protects the neighboring vascular cells against senescence by promoting rRNA transcription (By similarity).
See full target information ANG

Additional targets

FGF2,CXCL5,EGF,CXCL3,IFNG,IGF1,IL6,CXCL8,LEP,CCL2,PDGFB,PGF,CCL5,TGFB1,THPO,TIMP1,TIMP2,VEGFA,VEGFD
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Materials today. Bio 25:101001 PubMed38420144

2024

Assessing the functional potential of conditioned media derived from amniotic epithelial stem cells engineered on 3D biomimetic scaffolds: An in vitro model for tendon regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Valentina Russo,Giuseppe Prencipe,Annunziata Mauro,Mohammad El Khatib,Arlette A Haidar-Montes,Nico Cambise,Maura Turriani,Johannes Stöckl,Peter Steinberger,Loreto Lancia,Matthias Schnabelrauch,Paolo Berardinelli,Barbara Barboni

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2302561 PubMed37897317

2023

Mammary Microvessels are Sensitive to Menstrual Cycle Sex Hormones.

Applications

Unspecified application

Species

Unspecified reactive species

Carmen Moccia,Marta Cherubini,Marina Fortea,Akinola Akinbote,Prasanna Padmanaban,Violeta Beltran-Sastre,Kristina Haase

International journal of molecular sciences 24: PubMed36835400

2023

Endothelial Differentiation of Knockout iPSCs Triggers the Establishment of a Specific Gene Expression Signature.

Applications

Unspecified application

Species

Unspecified reactive species

Robin A Pilz,Dariush Skowronek,Lara Mellinger,Sander Bekeschus,Ute Felbor,Matthias Rath

PLoS pathogens 19:e1011077 PubMed36652443

2023

Ebola virus disrupts the inner blood-retinal barrier by induction of vascular endothelial growth factor in pericytes.

Applications

Unspecified application

Species

Unspecified reactive species

Jiawang Gao,Zhengyuan Guo,Wei Li,Xiaowei Zhang,Xian-En Zhang,Zongqiang Cui

Biomolecules 12: PubMed36139063

2022

Regenerative Potential of A Bovine ECM-Derived Hydrogel for Biomedical Applications.

Applications

Unspecified application

Species

Unspecified reactive species

Dalila Di Francesco,Fabio Bertani,Luca Fusaro,Nausicaa Clemente,Flavia Carton,Maria Talmon,Luigia Grazia Fresu,Francesca Boccafoschi

Scientific reports 12:8003 PubMed35568708

2022

Modelling liver cancer microenvironment using a novel 3D culture system.

Applications

Unspecified application

Species

Unspecified reactive species

Ala'a Al Hrout,Karla Cervantes-Gracia,Richard Chahwan,Amr Amin

Cells 11: PubMed35159271

2022

Hypoxia-Mimetic CoCl Agent Enhances Pro-Angiogenic Activities in Ovine Amniotic Epithelial Cells-Derived Conditioned Medium.

Applications

Unspecified application

Species

Unspecified reactive species

Miriam Di Mattia,Annunziata Mauro,Simona Delle Monache,Fanny Pulcini,Valentina Russo,Paolo Berardinelli,Maria Rita Citeroni,Maura Turriani,Alessia Peserico,Barbara Barboni

Frontiers in physiology 12:735915 PubMed34690810

2021

Classical and Non-classical Fibrosis Phenotypes Are Revealed by Lung and Cardiac Like Microvascular Tissues On-Chip.

Applications

Unspecified application

Species

Unspecified reactive species

Akinola Akinbote,Violeta Beltran-Sastre,Marta Cherubini,Roberta Visone,Cynthia Hajal,Defne Cobanoglu,Kristina Haase

Scientific reports 8:14733 PubMed30283098

2018

NF-κB-related decrease of glioma angiogenic potential by graphite nanoparticles and graphene oxide nanoplatelets.

Applications

Unspecified application

Species

Unspecified reactive species

Mateusz Wierzbicki,Ewa Sawosz,Barbara Strojny,Sławomir Jaworski,Marta Grodzik,André Chwalibog
View all publications
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