ab13400 is for simultaneous detection of 20 Human Angiogenic factors.
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- International journal of molecular sciences 24:2023Endothelial Differentiation of Knockout iPSCs Triggers the Establishment of a Specific Gene Expression Signature.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRobin A Pilz,Dariush Skowronek,Lara Mellinger,Sander Bekeschus,Ute Felbor,Matthias RathPubMed 36835400
- PLoS pathogens 19:e10110772023Ebola virus disrupts the inner blood-retinal barrier by induction of vascular endothelial growth factor in pericytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiawang Gao,Zhengyuan Guo,Wei Li,Xiaowei Zhang,Xian-En Zhang,Zongqiang CuiPubMed 36652443
- Scientific reports 12:80032022Modelling liver cancer microenvironment using a novel 3D culture system.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAla'a Al Hrout,Karla Cervantes-Gracia,Richard Chahwan,Amr AminPubMed 35568708
- Cells 11:2022Hypoxia-Mimetic CoCl Agent Enhances Pro-Angiogenic Activities in Ovine Amniotic Epithelial Cells-Derived Conditioned Medium.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMiriam Di Mattia,Annunziata Mauro,Simona Delle Monache,Fanny Pulcini,Valentina Russo,Paolo Berardinelli,Maria Rita Citeroni,Maura Turriani,Alessia Peserico,Barbara BarboniPubMed 35159271
- Frontiers in physiology 12:7359152021Classical and Non-classical Fibrosis Phenotypes Are Revealed by Lung and Cardiac Like Microvascular Tissues On-Chip.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAkinola Akinbote,Violeta Beltran-Sastre,Marta Cherubini,Roberta Visone,Cynthia Hajal,Defne Cobanoglu,Kristina HaasePubMed 34690810
- Scientific reports 8:147332018NF-κB-related decrease of glioma angiogenic potential by graphite nanoparticles and graphene oxide nanoplatelets.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMateusz Wierzbicki et. al.PubMed 30283098
Key facts
- Assay type
- Semi-quantitative
- Reactive species
- Human
- Target
- ANG
(See target data below)
Target data
Function
Secreted ribonuclease that can either promote or restrict cell proliferation of target cells, depending on the context (PubMed:12051708, PubMed:1400510, PubMed:19332886, PubMed:20129916, PubMed:21855800, PubMed:23047679, PubMed:23843625, PubMed:2424496, PubMed:2459697, PubMed:2730651, PubMed:27518564, PubMed:28176817, PubMed:29100074, PubMed:29748193, PubMed:3122207, PubMed:32510170, PubMed:38718836, PubMed:8159680, PubMed:8570639, PubMed:8622921, PubMed:9578571). Endocytosed in target cells via its receptor PLXNB2 and translocates to the cytoplasm or nucleus (PubMed:29100074, PubMed:32510170). Under stress conditions, localizes to the cytoplasm and promotes the assembly of stress granules (SGs): specifically cleaves a subset of tRNAs within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs), resulting in translation repression and inhibition of cell proliferation (PubMed:1400510, PubMed:19332886, PubMed:20129916, PubMed:21855800, PubMed:23047679, PubMed:27518564, PubMed:29100074, PubMed:29748193, PubMed:32510170, PubMed:38718836). tiRNas also prevent formation of apoptosome, thereby promoting cell survival (By similarity). Preferentially cleaves RNAs between a pyrimidine and an adenosine residue, suggesting that it cleaves the anticodon loop of tRNA(Ala) (32-UUAGCAU-38) after positions 33 and 36 (PubMed:3289612, PubMed:38718836). Cleaves a subset of tRNAs, including tRNA(Ala), tRNA(Glu), tRNA(Gly), tRNA(Lys), tRNA(Val), tRNA(His), tRNA(Asp) and tRNA(Sec) (PubMed:31582561). Under growth conditions and in differentiated cells, translocates to the nucleus and stimulates ribosomal RNA (rRNA) transcription, including that containing the initiation site sequences of 45S rRNA, thereby promoting cell growth and proliferation (PubMed:12051708, PubMed:15735021, PubMed:27518564, PubMed:29100074, PubMed:8127865). Angiogenin induces vascularization of normal and malignant tissues via its ability to promote rRNA transcription (PubMed:19354288, PubMed:4074709, PubMed:8448182). Involved in hematopoietic stem and progenitor cell (HSPC) growth and survival by promoting rRNA transcription in growth conditions and inhibiting translation in response to stress, respectively (PubMed:27518564). Mediates the crosstalk between myeloid and intestinal epithelial cells to protect the intestinal epithelial barrier integrity: secreted by myeloid cells and promotes intestinal epithelial cells proliferation and survival (PubMed:32510170). Also mediates osteoclast-endothelial cell crosstalk in growing bone: produced by osteoclasts and protects the neighboring vascular cells against senescence by promoting rRNA transcription (By similarity).
Additional Targets
FGF2, CXCL5, EGF, CXCL3, IFNG, IGF1, IL6, CXCL8, LEP, CCL2, PDGFB, PGF, CCL5, TGFB1, THPO, TIMP1, TIMP2, VEGFA, VEGFD
Alternative names
RNASE5, ANG, Angiogenin, Ribonuclease 5, RNase 5
What's included?
Recommended products
ab13400 is for simultaneous detection of 20 Human Angiogenic factors.
Key facts
- Assay type
- Semi-quantitative
- Reactive species
- Human
- Target
- ANG
- Applications
- Multiplex Protein Detection
- Detection method
- Luminescent
- Sample types
- Saliva, Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture media, Cell culture supernatant, Milk, Serum, Other biological fluids, Whole Blood, Cell Lysate
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
ab13400 is for simultaneous detection of 20 Human Angiogenic factors. Suitable for all sample types.
Targets: Angiogenin, EGF, ENA-78, bFGF, GRO, IFN-gamma, IGF-I, IL-6, IL-8, Leptin, MCP-1, PDGF-BB, PIGF, RANTES, TGF-beta1, TIMP-1, TIMP-2, Thrombopoietin, VEGF-A, VEGF-D
Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
If you are interested in this cytokine array, arrays ab133997, ab133998 and ab169819 may also be of interest. **A table listing all of our human membrane antibody cytokine arrays and other arrays and the analytes they measure is available .**"
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Multiplex Protein Detection - Human Angiogenesis Antibody Array - Membrane (20 Targets) (ab134000)
Typical results obtained with Abcam Human Cytokine Antibody Array - Membrane. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at RT for 1 min.
ELISA - Human Angiogenesis Antibody Array - Membrane (20 Targets) (ab134000)
THP-1 cells (Human peripheral blood monocytes) were seeded at 1x106 cells/mL and cultured in RPMI media supplemented with 10% fetal calf serum, 0.05mM 2-mercaptoethanol, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were cultured for 2 days in the presence of LPS. Conditioned media was harvested after 48 hours post-stimulation, aliquoted and assayed using ab134000. Mean pixel density was quantified using CCD camera software analysis.
HUVEC cells (Human umbilical vein endothelial cells) were seeded at 1x106 cells/mL and cultured in RPMI media supplemented with 10% fetal calf serum, 0.1 mg/mL heparin, 0.05 mg/mL ECGS, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab134000. Mean pixel density was quantified using CCD camera software analysis.
ELISA - Human Angiogenesis Antibody Array - Membrane (20 Targets) (ab134000)
Human serum and plasma (EDTA) from a pooled donor (n=50) sample was diluted to 25% and assayed using ab134000. Mean pixel density was quantified using CCD camera software analysis.
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