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AB169808

Human Angiogenesis Antibody Array - Membrane (23 targets)

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(2 Publications)

ab169808 is for simultaneous detection of 23 Human Angiogenic Factors.

View Alternative Names

KIAA0003, ANGPT1, Angiopoietin-1, ANG-1, Plasminogen, PLG, ITAC, SCYB11, SCYB9B, CXCL11, C-X-C motif chemokine 11, Beta-R1, H174, Interferon gamma-inducible protein 9, Interferon-inducible T-cell alpha chemoattractant, Small-inducible cytokine B11, IP-9, I-TAC, CD87, MO3, UPAR, PLAUR, Urokinase plasminogen activator surface receptor, U-PAR, uPAR, Monocyte activation antigen Mo3, CD202b, TIE2, VMCM, VMCM1, TEK, Angiopoietin-1 receptor, Endothelial tyrosine kinase, Tunica interna endothelial cell kinase, Tyrosine kinase with Ig and EGF homology domains-2, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE-2, p140 TEK, hTIE2, CD309, FLK1, VEGFR2, KDR, Vascular endothelial growth factor receptor 2, VEGFR-2, Fetal liver kinase 1, Kinase insert domain receptor, Protein-tyrosine kinase receptor flk-1, FLK-1, VEGFR3, FLT4, Vascular endothelial growth factor receptor 3, VEGFR-3, Fms-like tyrosine kinase 4, Tyrosine-protein kinase receptor FLT4, FLT-4, C17orf33, GCSF, CSF3, Granulocyte colony-stimulating factor, G-CSF, Pluripoietin, CD31, Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, PECAM1, MCP4, NCC1, SCYA13, CCL13, C-C motif chemokine 13, CK-beta-10, Monocyte chemoattractant protein 4, Monocyte chemotactic protein 4, NCC-1, Small-inducible cytokine A13, MCP-4, Collagen alpha-1(XVIII) chain, COL18A1, SCYA1, CCL1, C-C motif chemokine 1, Small-inducible cytokine A1, T lymphocyte-secreted protein I-309, Interleukin-2, IL-2, T-cell growth factor, TCGF, IL2, Interleukin-4, IL-4, B-cell stimulatory factor 1, Binetrakin, Lymphocyte stimulatory factor 1, Pitrakinra, BSF-1, IL4, IL1F2, IL1B, Interleukin-1 beta, IL-1 beta, Catabolin, TNFA, TNFSF2, TNF, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Angiopoietin-2, ANG-2, ANGPT2, GMCSF, CSF2, Granulocyte-macrophage colony-stimulating factor, GM-CSF, Colony-stimulating factor, Molgramostin, Sargramostim, CSF, IL1F1, IL1A, Interleukin-1 alpha, IL-1 alpha, Hematopoietin-1, Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10, MCP3, SCYA6, SCYA7, CCL7, C-C motif chemokine 7, Monocyte chemoattractant protein 3, Monocyte chemotactic protein 3, NC28, Small-inducible cytokine A7, MCP-3, CLG, MMP1, Interstitial collagenase, Fibroblast collagenase, Matrix metalloproteinase-1, MMP-1

1 Images
Multiplex Protein Detection - Human Angiogenesis Antibody Array - Membrane (23 targets) (AB169808)
  • Multiplex Protein Detection

Unknown

Multiplex Protein Detection - Human Angiogenesis Antibody Array - Membrane (23 targets) (AB169808)

Typical results obtained with Abcam Membrane Antibody Arrays. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at room temperature for 1 minute.

Key facts

Reacts with

Human

Target

ANGPT1

target

Applications

Multiplex Protein Detection

applications

Detection method

Luminescent

Sample types

Saliva, Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture media, Cell culture supernatant, Milk, Serum, Other biological fluids, Whole Blood, Cell Lysate

Reactivity data

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Product details

ab169808 is for simultaneous detection of 23 Human Angiogenic Factors. Suitable for all liquid sample types.

Targets: Angiopoietin 1, Angiopoietin 2, Angiostatin, Endostatin, G-CSF, GM-CSF, I-309, IL-10, IL-1 alpha, IL-1 beta, IL-2, IL-4, I-TAC, MCP-3, MCP-4, MMP-1, MMP-9, PECAM-1, Tie-2, TNF alpha, uPAR, VEGFR2, VEGFR3

Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.

A table listing all of our human membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Product protocols

Target data

Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme.
See full target information ANGPT1

Additional targets

ANGPT2,PLG,COL18A1,CSF3,CSF2,CCL1,IL1A,IL1B,IL10,IL2,IL4,CXCL11,CCL7,CCL13,MMP1,PECAM1,TEK,TNF,PLAUR,KDR,FLT4

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 12:8003 PubMed35568708

2022

Modelling liver cancer microenvironment using a novel 3D culture system.

Applications

Unspecified application

Species

Unspecified reactive species

Ala'a Al Hrout,Karla Cervantes-Gracia,Richard Chahwan,Amr Amin

Frontiers in cardiovascular medicine 5:108 PubMed30159316

2018

Serum Biomarkers of Endothelial Dysfunction in Fabry Associated Cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Jefferson Loso,Natalie Lund,Maxim Avanesov,Nicole Muschol,Susanne Lezius,Kathrin Cordts,Edzard Schwedhelm,Monica Patten
View all publications
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