Skip to main content

ab193655 can be used for simultaneous detection of 43 Human Angiogenesis proteins in serum, plasma, cell culture media and other liquid sample types.

Be the first to review this product! Submit a review

Images

Multiplex Protein Detection - Human Angiogenesis Antibody Array - Membrane (43 Targets) (AB193655), expandable thumbnail

Publications

Key facts

Assay type
Semi-quantitative
Reactive species
Human
Target
ANG
(See target data below)
Assay Platform
Microplate

Target data

Function

Secreted ribonuclease that can either promote or restrict cell proliferation of target cells, depending on the context (PubMed:12051708, PubMed:1400510, PubMed:19332886, PubMed:20129916, PubMed:21855800, PubMed:23047679, PubMed:23843625, PubMed:2424496, PubMed:2459697, PubMed:2730651, PubMed:27518564, PubMed:28176817, PubMed:29100074, PubMed:29748193, PubMed:3122207, PubMed:32510170, PubMed:38718836, PubMed:8159680, PubMed:8570639, PubMed:8622921, PubMed:9578571). Endocytosed in target cells via its receptor PLXNB2 and translocates to the cytoplasm or nucleus (PubMed:29100074, PubMed:32510170). Under stress conditions, localizes to the cytoplasm and promotes the assembly of stress granules (SGs): specifically cleaves a subset of tRNAs within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs), resulting in translation repression and inhibition of cell proliferation (PubMed:1400510, PubMed:19332886, PubMed:20129916, PubMed:21855800, PubMed:23047679, PubMed:27518564, PubMed:29100074, PubMed:29748193, PubMed:32510170, PubMed:38718836). tiRNas also prevent formation of apoptosome, thereby promoting cell survival (By similarity). Preferentially cleaves RNAs between a pyrimidine and an adenosine residue, suggesting that it cleaves the anticodon loop of tRNA(Ala) (32-UUAGCAU-38) after positions 33 and 36 (PubMed:3289612, PubMed:38718836). Cleaves a subset of tRNAs, including tRNA(Ala), tRNA(Glu), tRNA(Gly), tRNA(Lys), tRNA(Val), tRNA(His), tRNA(Asp) and tRNA(Sec) (PubMed:31582561). Under growth conditions and in differentiated cells, translocates to the nucleus and stimulates ribosomal RNA (rRNA) transcription, including that containing the initiation site sequences of 45S rRNA, thereby promoting cell growth and proliferation (PubMed:12051708, PubMed:15735021, PubMed:27518564, PubMed:29100074, PubMed:8127865). Angiogenin induces vascularization of normal and malignant tissues via its ability to promote rRNA transcription (PubMed:19354288, PubMed:4074709, PubMed:8448182). Involved in hematopoietic stem and progenitor cell (HSPC) growth and survival by promoting rRNA transcription in growth conditions and inhibiting translation in response to stress, respectively (PubMed:27518564). Mediates the crosstalk between myeloid and intestinal epithelial cells to protect the intestinal epithelial barrier integrity: secreted by myeloid cells and promotes intestinal epithelial cells proliferation and survival (PubMed:32510170). Also mediates osteoclast-endothelial cell crosstalk in growing bone: produced by osteoclasts and protects the neighboring vascular cells against senescence by promoting rRNA transcription (By similarity).

Additional Targets

ANGPT1, ANGPT2, PLG, FGF2, CXCL5, EGF, COL18A1, CSF3, CSF2, CXCL3, CCL1, IFNG, IGF1, IL1A, IL1B, IL10, IL2, IL4, IL6, CXCL8, CXCL11, LEP, CCL2, CCL7, CCL13, MMP1, PDGFB, PECAM1, PGF, CCL5, TGFB1, THPO, TEK, TIMP1, TIMP2, TNF, PLAUR, VEGFA, VEGFD, KDR, FLT4

Alternative names

RNASE5, ANG, Angiogenin, Ribonuclease 5, RNase 5

What's included?

1 x 8 Membranes
Components
1,000X HRP-Conjugated Streptavidin
2 x 50 µL
1X Blocking Buffer
4 x 25 mL
20X Wash Buffer I
2 x 20 mL
20X Wash Buffer II
2 x 20 mL
2X Cell Lysis Buffer
2 x 16 mL
8-Well Incubation Tray (with Lid)
2 x 1 Unit
Biotinylated Antibody Cocktail (C1)
4 x 1 Vial
Biotinylated Antibody Cocktail (C2)
4 x 1 Vial
Detection Buffer C
2 x 2.5 mL
Detection Buffer D
2 x 2.5 mL
Human Angiogenesis Antibody Array Membranes (C1)
2 x 4 Unit
Human Angiogenesis Antibody Array Membranes (C2)
2 x 4 Unit
Plastic Sheets
2 x 1 Unit

Recommended products

ab193655 can be used for simultaneous detection of 43 Human Angiogenesis proteins in serum, plasma, cell culture media and other liquid sample types.

Key facts

Assay type
Semi-quantitative
Reactive species
Human
Target
ANG
Applications
Multiplex Protein Detection
Detection method
Luminescent
Assay Platform
Microplate
Sample types
Tissue Lysate, Plasma, Cell culture media, Serum, Other biological fluids, Cell Lysate

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

ab193655 can be used for simultaneous detection of 43 Human Angiogenesis proteins in serum, plasma, cell culture media and other liquid sample types. It comes as 1 kit including 2 separate membranes labelled C1 and C2 (see below for images showing the array layout on each membrane).

Targets: Angiogenin, Angiopoietin-1, Angiopoietin-2, Angiostatin, bFGF, EGF, ENA-78 (CXCL5), Endostatin, GCSF, GM-CSF, GRO alpha, GRO beta, GRO gamma, I-309 (TCA-3/CCL1), IFN-gamma, IGF-1, IL-10, IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-2, IL-4, IL-6, IL-8 (CXCL8), I-TAC (CXCL11), Leptin, MCP-1 (CCL2), MCP-3 (MARC/CCL7), MCP-4 (CCL13), MMP-1, MMP-9, PDGF-BB, PECAM-1 (CD31), PLGF, RANTES (CCL5), TGF beta 1, Thrombopoietin (TPO), Tie-2,TIMP-1, TIMP-2, TNF alpha, uPAR, VEGF-A, VEGFR2, VEGFR3, VEGF-D.


If you are interested in this cytokine array, arrays ab133998, ab133997, ab134000, ab169808 and ab169804 may also be of interest. A list of human antibody membrane arrays is available .


Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Multiplex Protein Detection - Human Angiogenesis Antibody Array - Membrane (43 Targets) (ab193655), expandable thumbnail

    Multiplex Protein Detection - Human Angiogenesis Antibody Array - Membrane (43 Targets) (ab193655)

    Typical images obtained with Abcam Human Angiogenesis Antibody Array - Membrane C2 (43 Targets) ab193655. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at room temperature for 1 minute.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com