Human Apoptosis Antibody Array - Membrane (43 Targets)
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(75 Publications)
Human Apoptosis Antibody Array - Membrane (43 Targets) ab134001 is a membrane based multiplex immunoassay for the simultaneous detection of 43 human apoptotic markers in all sample types.
- More data, less sample: screen multiple apoptotic markers with the same sample
- Global view of apoptosis
- Chemiluminescent detection - no specific equipment required
- Highly sensitive (pg/mL)
View Alternative Names
BBC6, BCL2L8, BAD, Bcl2-associated agonist of cell death, Bcl-2-binding component 6, Bcl-2-like protein 8, Bcl-xL/Bcl-2-associated death promoter, Bcl2 antagonist of cell death, Bcl2-L-8, CD40, TNFRSF5, Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40, CD358, DR6, UNQ437/PRO868, TNFRSF21, Tumor necrosis factor receptor superfamily member 21, Death receptor 6, HSP72, HSPA1, HSX70, HSPA1A, Heat shock 70 kDa protein 1A, Heat shock 70 kDa protein 1, Heat shock protein family A member 1A, HSP70-1, HSP70.1, BP2, IBP2, IGFBP2, Insulin-like growth factor-binding protein 2, IBP-2, IGF-binding protein 2, IGFBP-2, CD120a, TNFAR, TNFR1, TNFRSF1A, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor 1, Tumor necrosis factor receptor type I, p55, p60, TNF-R1, TNF-RI, TNFR-I, CD221, Insulin-like growth factor 1 receptor, Insulin-like growth factor I receptor, IGF-I receptor, IGF1R, HSP60, HSPD1, 60 kDa chaperonin, Chaperonin 60, Heat shock protein 60, Heat shock protein family D member 1, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, CPN60, HSP-60, Hsp60, CD262, DR5, KILLER, TRAILR2, TRICK2, ZTNFR9, UNQ160/PRO186, TNFRSF10B, Tumor necrosis factor receptor superfamily member 10B, Death receptor 5, TNF-related apoptosis-inducing ligand receptor 2, TRAIL receptor 2, TRAIL-R2, BCL2L4, BAX, Apoptosis regulator BAX, Bcl-2-like protein 4, Bcl2-L-4, API4, IAP4, BIRC5, Baculoviral IAP repeat-containing protein 5, Apoptosis inhibitor 4, Apoptosis inhibitor survivin, HSP72, HSPA1B, Heat shock 70 kDa protein 1B, Heat shock 70 kDa protein 2, Heat shock protein family A member 1B, HSP70-2, HSP70.2, IBP4, IGFBP4, Insulin-like growth factor-binding protein 4, IBP-4, IGF-binding protein 4, IGFBP-4, CD95, APT1, FAS1, TNFRSF6, FAS, Tumor necrosis factor receptor superfamily member 6, Apo-1 antigen, Apoptosis-mediating surface antigen FAS, FASLG receptor, CD261, APO2, DR4, TRAILR1, TNFRSF10A, Tumor necrosis factor receptor superfamily member 10A, Death receptor 4, TNF-related apoptosis-inducing ligand receptor 1, TRAIL receptor 1, TRAIL-R1, P53, TP53, Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, KIP1, p27, CDKN1B, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, Apoptosis regulator Bcl-2, BCL2, CPP32, CASP3, Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, Protein Yama, SREBP cleavage activity 1, CPP-32, SCA-1, OMI, PRSS25, HTRA2, High temperature requirement protein A2, Omi stress-regulated endoprotease, Serine protease 25, Serine proteinase OMI, HtrA2, TNFA, TNFSF2, TNF, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, APG2, HSPH2, HSPA4, Heat shock 70 kDa protein 4, HSP70RY, Heat shock 70-related protein APG-2, Heat shock protein family H member 2, CD178, APT1LG1, CD95L, FASL, TNFSF6, FASLG, Tumor necrosis factor ligand superfamily member 6, Apoptosis antigen ligand, CD95 ligand, Fas antigen ligand, APTL, CD95-L, Fas ligand, FasL, IBP1, IGF-1, IGF1, Insulin-like growth factor 1, Insulin-like growth factor I, Mechano growth factor, Somatomedin-C, IGF-I, MGF, IBP1, IGFBP1, Insulin-like growth factor-binding protein 1, IBP-1, IGF-binding protein 1, IGFBP-1, Placental protein 12, PP12, IBP3, IGFBP3, Insulin-like growth factor-binding protein 3, IBP-3, IGF-binding protein 3, IGFBP-3, TNFB, TNFSF1, LTA, Lymphotoxin-alpha, LT-alpha, TNF-beta, Tumor necrosis factor ligand superfamily member 1, CD263, DCR1, LIT, TRAILR3, TRID, UNQ321/PRO366, TNFRSF10C, Tumor necrosis factor receptor superfamily member 10C, Antagonist decoy receptor for TRAIL/Apo-2L, Decoy TRAIL receptor without death domain, Decoy receptor 1, Lymphocyte inhibitor of TRAIL, TNF-related apoptosis-inducing ligand receptor 3, TRAIL receptor without an intracellular domain, DcR1, TRAIL receptor 3, TRAIL-R3, CD264, DCR2, TRAILR4, TRUNDD, UNQ251/PRO288, TNFRSF10D, Tumor necrosis factor receptor superfamily member 10D, Decoy receptor 2, TNF-related apoptosis-inducing ligand receptor 4, TRAIL receptor with a truncated death domain, DcR2, TRAIL receptor 4, TRAIL-R4, IGF-2, PP1446, IGF2, Insulin-like growth factor 2, Insulin-like growth factor II, Somatomedin-A, T3M-11-derived growth factor, IGF-II, SMAC, DIABLO, Diablo IAP-binding mitochondrial protein, Direct IAP-binding protein with low pI, Second mitochondria-derived activator of caspases, API3, BIRC4, IAP3, XIAP, E3 ubiquitin-protein ligase XIAP, Baculoviral IAP repeat-containing protein 4, IAP-like protein, Inhibitor of apoptosis protein 3, RING-type E3 ubiquitin transferase XIAP, X-linked inhibitor of apoptosis protein, ILP, hILP, IAP-3, hIAP-3, hIAP3, X-linked IAP, CD154, CD40L, TNFSF5, TRAP, CD40LG, CD40 ligand, CD40-L, T-cell antigen Gp39, TNF-related activation protein, Tumor necrosis factor ligand superfamily member 5, HSP27, HSP28, HSPB1, Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, Heat shock protein family B member 1, Stress-responsive protein 27, HSP 27, SRP27, BIM, BCL2L11, Bcl-2-like protein 11, Bcl2-L-11, Bcl2-interacting mediator of cell death, CD120b, TNFBR, TNFR2, TNFRSF1B, Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor 2, Tumor necrosis factor receptor type II, p75, p80 TNF-alpha receptor, TNF-R2, TNF-RII, TNFR-II, IBP6, IGFBP6, Insulin-like growth factor-binding protein 6, IBP-6, IGF-binding protein 6, IGFBP-6, API2, MIHC, RNF49, BIRC3, Baculoviral IAP repeat-containing protein 3, Apoptosis inhibitor 2, Cellular inhibitor of apoptosis 2, IAP homolog C, Inhibitor of apoptosis protein 1, RING finger protein 49, RING-type E3 ubiquitin transferase BIRC3, TNFR2-TRAF-signaling complex protein 1, C-IAP2, hIAP-1, hIAP1, CYC, CYCS, Cytochrome c, MCH5, CASP8, Caspase-8, CASP-8, Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4, FADD-homologous ICE/ced-3-like protease, FADD-like ICE, ICE-like apoptotic protease 5, MORT1-associated ced-3 homolog, FLICE, MACH, KIAP, LIVIN, MLIAP, RNF50, UNQ5800/PRO19607/PRO21344, BIRC7, Baculoviral IAP repeat-containing protein 7, Kidney inhibitor of apoptosis protein, Livin, Melanoma inhibitor of apoptosis protein, RING finger protein 50, RING-type E3 ubiquitin transferase BIRC7, ML-IAP, BH3-interacting domain death agonist, p22 BID, BID, CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1, CDKN1A, Cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, Melanoma differentiation-associated protein 6, p21, MDA-6, BCLW, KIAA0271, BCL2L2, Bcl-2-like protein 2, Bcl2-L-2, Apoptosis regulator Bcl-W, IBP5, IGFBP5, Insulin-like growth factor-binding protein 5, IBP-5, IGF-binding protein 5, IGFBP-5
- Multiplex Protein Detection
Unknown
Multiplex Protein Detection - Human Apoptosis Antibody Array - Membrane (43 Targets) (AB134001)
Multiplex Protein Detection - Human Apoptosis Antibody Array - Membrane (43 Targets).
Apoptotic protein profiling in Staurosporine-treated and untreated Jurkat cell lines
107 Jurkat cells were untreated or treated with staurosporine (0.5 μM) for 3 hours. Equal total lysate protein was loaded on each Abcam Apoptosis Antibody Array membrane. Antibody spots exhibiting signal differences are indicated in red boxes.
Using tissue or cell lysate samples may mask the positive control spots. This would need additional optimization steps.
- Array
Supplier Data
Array - Human Apoptosis Antibody Array - Membrane (43 Targets) (AB134001)
Array using human serum, tested at a 5x dilution
Reactivity data
Product details
ab134001 is for simultaneous detection of 43 Human Apoptotic markers.
Targets: Bad, Bax, Bcl-2, Bcl-w, BID, BIM, Caspase3, caspase8, CD40, CD40L, cIAP-2, cytoC, DR6, Fas, FasL, HSP27, HSP60, HSP70, HTRA, IGF-I, IGF-II, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-5, IGFBP-6, IGF-1sR, livin, p21, p27, p53, SMAC, Survivin, sTNF-R1, sTNF-R2, TNF-alpha, TNF-beta, TRAILR-1, TRAILR-2, TRAILR-3, TRAILR-4, XIAP
Caspase 3-will detect only the active form
Caspase 8-will detect the full length pro-form and the active form.
Antibody arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
We do not recommend cell and tissue lysates for the ab134001 as it may mask the positive control spots.
Using tissue or cell lysate samples may mask the positive control spots. This would need additional optimization steps.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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Properties and storage information
Shipped at conditions
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Appropriate long-term storage conditions
Storage information
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Additional targets
Publications (75)
Recent publications for all applications. Explore the full list and refine your search
Investigational new drugs 43:167-179 PubMed39875774
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Stem cell research & therapy 15:363 PubMed39396003
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RSC medicinal chemistry : PubMed39263684
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Stem cell research & therapy 15:189 PubMed38956646
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Cancer gene therapy 31:1164-1176 PubMed38918490
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Cancer cell international 24:207 PubMed38867223
2024
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Molecules (Basel, Switzerland) 29: PubMed38792269
2024
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Cell death & disease 15:255 PubMed38600086
2024
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Biomolecules & biomedicine 24:323-336 PubMed37540586
2024
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ChemMedChem 19:e202300680 PubMed38323458
2024
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