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ab134001 is for simultaneous detection of 43 Human Apoptotic markers.


Images

Multiplex Protein Detection - Human Apoptosis Antibody Array - Membrane (43 Targets) (AB134001), expandable thumbnail
  • Array - Human Apoptosis Antibody Array - Membrane (43 Targets) (AB134001), expandable thumbnail

Publications

Key facts

Assay type
Semi-quantitative
Reactive species
Human
Target
BAD
(See target data below)

Target data

Function

Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.

Additional Targets

BAX, BCL2, BCL2L2, BID, BCL2L11, CASP3, CASP8, CD40, CD40LG, BIRC3, CYCS, TNFRSF21, FAS, FASLG, HSPB1, HSPD1, HSPA4, HTRA2, IGF1, IGF1R, IGF2, IGFBP1, IGFBP2, IGFBP3, IGFBP4, IGFBP5, IGFBP6, BIRC7, CDKN1A, CDKN1B, TP53, DIABLO, BIRC5, TNF, LTA, TNFRSF1A, TNFRSF1B, TNFRSF10A, TNFRSF10B, TNFRSF10C, TNFRSF10D, XIAP

Alternative names

BBC6, BCL2L8, BAD, Bcl2-associated agonist of cell death, Bcl-2-binding component 6, Bcl-2-like protein 8, Bcl-xL/Bcl-2-associated death promoter, Bcl2 antagonist of cell death, Bcl2-L-8

What's included?

1 x 8 Membranes
Components
1,000X HRP-Conjugated Streptavidin
1 x 50 µL
1X Blocking Buffer
2 x 25 mL
20X Wash Buffer I
1 x 20 mL
20X Wash Buffer II
1 x 20 mL
2X Cell Lysis Buffer
1 x 16 mL
8-Well Incubation Tray (with Lid)
1 x 1 Unit
Apoptosis Antibody Array Membranes
2 x 4 Unit
Biotin-Conjugated Anti-Cytokines
4 x 1 Vial
Detection Buffer C
1 x 2.5 mL
Detection Buffer D
1 x 2.5 mL
Plastic sheets
1 x 1 Unit
Protease Inhibitor Cocktail
2 x 1 Unit

Recommended products

ab134001 is for simultaneous detection of 43 Human Apoptotic markers.

Key facts

Assay type
Semi-quantitative
Reactive species
Human
Target
BAD
Applications
Multiplex Protein Detection
Sample types
Cell culture supernatant, Plasma, Serum

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

ab134001 is for simultaneous detection of 43 Human Apoptotic markers.

Targets: Bad, Bax, Bcl-2, Bcl-w, BID, BIM, Caspase3, caspase8, CD40, CD40L, cIAP-2, cytoC, DR6, Fas, FasL, HSP27, HSP60, HSP70, HTRA, IGF-I, IGF-II, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-5, IGFBP-6, IGF-1sR, livin, p21, p27, p53, SMAC, Survivin, sTNF-R1, sTNF-R2, TNF-alpha, TNF-beta, TRAILR-1, TRAILR-2, TRAILR-3, TRAILR-4, XIAP

Caspase 3-will detect only the active form

Caspase 8-will detect the full length pro-form and the active form.

Antibody arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.

We do not recommend cell and tissue lysates for the ab134001 as it may mask the positive control spots.


Using tissue or cell lysate samples may mask the positive control spots. This would need additional optimization steps.


Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Multiplex Protein Detection - Human Apoptosis Antibody Array - Membrane (43 Targets) (ab134001), expandable thumbnail

    Multiplex Protein Detection - Human Apoptosis Antibody Array - Membrane (43 Targets) (ab134001)

    Multiplex Protein Detection - Human Apoptosis Antibody Array - Membrane (43 Targets).

    Apoptotic protein profiling in Staurosporine-treated and untreated Jurkat cell lines

    107 Jurkat cells were untreated or treated with staurosporine (0.5 μM) for 3 hours. Equal total lysate protein was loaded on each Abcam Apoptosis Antibody Array membrane. Antibody spots exhibiting signal differences are indicated in red boxes.

    Using tissue or cell lysate samples may mask the positive control spots. This would need additional optimization steps.

  • Array - Human Apoptosis Antibody Array - Membrane (43 Targets) (ab134001), expandable thumbnail

    Array - Human Apoptosis Antibody Array - Membrane (43 Targets) (ab134001)

    Array using human serum, tested at a 5x dilution

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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