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AB134001

Human Apoptosis Antibody Array - Membrane (43 Targets)

1

(1 Review)

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(75 Publications)

Human Apoptosis Antibody Array - Membrane (43 Targets) ab134001 is a membrane based multiplex immunoassay for the simultaneous detection of 43 human apoptotic markers in all sample types.

- More data, less sample: screen multiple apoptotic markers with the same sample
- Global view of apoptosis
- Chemiluminescent detection - no specific equipment required
- Highly sensitive (pg/mL)

View Alternative Names

BBC6, BCL2L8, BAD, Bcl2-associated agonist of cell death, Bcl-2-binding component 6, Bcl-2-like protein 8, Bcl-xL/Bcl-2-associated death promoter, Bcl2 antagonist of cell death, Bcl2-L-8, CD40, TNFRSF5, Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40, CD358, DR6, UNQ437/PRO868, TNFRSF21, Tumor necrosis factor receptor superfamily member 21, Death receptor 6, HSP72, HSPA1, HSX70, HSPA1A, Heat shock 70 kDa protein 1A, Heat shock 70 kDa protein 1, Heat shock protein family A member 1A, HSP70-1, HSP70.1, BP2, IBP2, IGFBP2, Insulin-like growth factor-binding protein 2, IBP-2, IGF-binding protein 2, IGFBP-2, CD120a, TNFAR, TNFR1, TNFRSF1A, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor 1, Tumor necrosis factor receptor type I, p55, p60, TNF-R1, TNF-RI, TNFR-I, CD221, Insulin-like growth factor 1 receptor, Insulin-like growth factor I receptor, IGF-I receptor, IGF1R, HSP60, HSPD1, 60 kDa chaperonin, Chaperonin 60, Heat shock protein 60, Heat shock protein family D member 1, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, CPN60, HSP-60, Hsp60, CD262, DR5, KILLER, TRAILR2, TRICK2, ZTNFR9, UNQ160/PRO186, TNFRSF10B, Tumor necrosis factor receptor superfamily member 10B, Death receptor 5, TNF-related apoptosis-inducing ligand receptor 2, TRAIL receptor 2, TRAIL-R2, BCL2L4, BAX, Apoptosis regulator BAX, Bcl-2-like protein 4, Bcl2-L-4, API4, IAP4, BIRC5, Baculoviral IAP repeat-containing protein 5, Apoptosis inhibitor 4, Apoptosis inhibitor survivin, HSP72, HSPA1B, Heat shock 70 kDa protein 1B, Heat shock 70 kDa protein 2, Heat shock protein family A member 1B, HSP70-2, HSP70.2, IBP4, IGFBP4, Insulin-like growth factor-binding protein 4, IBP-4, IGF-binding protein 4, IGFBP-4, CD95, APT1, FAS1, TNFRSF6, FAS, Tumor necrosis factor receptor superfamily member 6, Apo-1 antigen, Apoptosis-mediating surface antigen FAS, FASLG receptor, CD261, APO2, DR4, TRAILR1, TNFRSF10A, Tumor necrosis factor receptor superfamily member 10A, Death receptor 4, TNF-related apoptosis-inducing ligand receptor 1, TRAIL receptor 1, TRAIL-R1, P53, TP53, Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, KIP1, p27, CDKN1B, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, Apoptosis regulator Bcl-2, BCL2, CPP32, CASP3, Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, Protein Yama, SREBP cleavage activity 1, CPP-32, SCA-1, OMI, PRSS25, HTRA2, High temperature requirement protein A2, Omi stress-regulated endoprotease, Serine protease 25, Serine proteinase OMI, HtrA2, TNFA, TNFSF2, TNF, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, APG2, HSPH2, HSPA4, Heat shock 70 kDa protein 4, HSP70RY, Heat shock 70-related protein APG-2, Heat shock protein family H member 2, CD178, APT1LG1, CD95L, FASL, TNFSF6, FASLG, Tumor necrosis factor ligand superfamily member 6, Apoptosis antigen ligand, CD95 ligand, Fas antigen ligand, APTL, CD95-L, Fas ligand, FasL, IBP1, IGF-1, IGF1, Insulin-like growth factor 1, Insulin-like growth factor I, Mechano growth factor, Somatomedin-C, IGF-I, MGF, IBP1, IGFBP1, Insulin-like growth factor-binding protein 1, IBP-1, IGF-binding protein 1, IGFBP-1, Placental protein 12, PP12, IBP3, IGFBP3, Insulin-like growth factor-binding protein 3, IBP-3, IGF-binding protein 3, IGFBP-3, TNFB, TNFSF1, LTA, Lymphotoxin-alpha, LT-alpha, TNF-beta, Tumor necrosis factor ligand superfamily member 1, CD263, DCR1, LIT, TRAILR3, TRID, UNQ321/PRO366, TNFRSF10C, Tumor necrosis factor receptor superfamily member 10C, Antagonist decoy receptor for TRAIL/Apo-2L, Decoy TRAIL receptor without death domain, Decoy receptor 1, Lymphocyte inhibitor of TRAIL, TNF-related apoptosis-inducing ligand receptor 3, TRAIL receptor without an intracellular domain, DcR1, TRAIL receptor 3, TRAIL-R3, CD264, DCR2, TRAILR4, TRUNDD, UNQ251/PRO288, TNFRSF10D, Tumor necrosis factor receptor superfamily member 10D, Decoy receptor 2, TNF-related apoptosis-inducing ligand receptor 4, TRAIL receptor with a truncated death domain, DcR2, TRAIL receptor 4, TRAIL-R4, IGF-2, PP1446, IGF2, Insulin-like growth factor 2, Insulin-like growth factor II, Somatomedin-A, T3M-11-derived growth factor, IGF-II, SMAC, DIABLO, Diablo IAP-binding mitochondrial protein, Direct IAP-binding protein with low pI, Second mitochondria-derived activator of caspases, API3, BIRC4, IAP3, XIAP, E3 ubiquitin-protein ligase XIAP, Baculoviral IAP repeat-containing protein 4, IAP-like protein, Inhibitor of apoptosis protein 3, RING-type E3 ubiquitin transferase XIAP, X-linked inhibitor of apoptosis protein, ILP, hILP, IAP-3, hIAP-3, hIAP3, X-linked IAP, CD154, CD40L, TNFSF5, TRAP, CD40LG, CD40 ligand, CD40-L, T-cell antigen Gp39, TNF-related activation protein, Tumor necrosis factor ligand superfamily member 5, HSP27, HSP28, HSPB1, Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, Heat shock protein family B member 1, Stress-responsive protein 27, HSP 27, SRP27, BIM, BCL2L11, Bcl-2-like protein 11, Bcl2-L-11, Bcl2-interacting mediator of cell death, CD120b, TNFBR, TNFR2, TNFRSF1B, Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor 2, Tumor necrosis factor receptor type II, p75, p80 TNF-alpha receptor, TNF-R2, TNF-RII, TNFR-II, IBP6, IGFBP6, Insulin-like growth factor-binding protein 6, IBP-6, IGF-binding protein 6, IGFBP-6, API2, MIHC, RNF49, BIRC3, Baculoviral IAP repeat-containing protein 3, Apoptosis inhibitor 2, Cellular inhibitor of apoptosis 2, IAP homolog C, Inhibitor of apoptosis protein 1, RING finger protein 49, RING-type E3 ubiquitin transferase BIRC3, TNFR2-TRAF-signaling complex protein 1, C-IAP2, hIAP-1, hIAP1, CYC, CYCS, Cytochrome c, MCH5, CASP8, Caspase-8, CASP-8, Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4, FADD-homologous ICE/ced-3-like protease, FADD-like ICE, ICE-like apoptotic protease 5, MORT1-associated ced-3 homolog, FLICE, MACH, KIAP, LIVIN, MLIAP, RNF50, UNQ5800/PRO19607/PRO21344, BIRC7, Baculoviral IAP repeat-containing protein 7, Kidney inhibitor of apoptosis protein, Livin, Melanoma inhibitor of apoptosis protein, RING finger protein 50, RING-type E3 ubiquitin transferase BIRC7, ML-IAP, BH3-interacting domain death agonist, p22 BID, BID, CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1, CDKN1A, Cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, Melanoma differentiation-associated protein 6, p21, MDA-6, BCLW, KIAA0271, BCL2L2, Bcl-2-like protein 2, Bcl2-L-2, Apoptosis regulator Bcl-W, IBP5, IGFBP5, Insulin-like growth factor-binding protein 5, IBP-5, IGF-binding protein 5, IGFBP-5

2 Images
Multiplex Protein Detection - Human Apoptosis Antibody Array - Membrane (43 Targets) (AB134001)
  • Multiplex Protein Detection

Unknown

Multiplex Protein Detection - Human Apoptosis Antibody Array - Membrane (43 Targets) (AB134001)

Multiplex Protein Detection - Human Apoptosis Antibody Array - Membrane (43 Targets).

Apoptotic protein profiling in Staurosporine-treated and untreated Jurkat cell lines

107 Jurkat cells were untreated or treated with staurosporine (0.5 μM) for 3 hours. Equal total lysate protein was loaded on each Abcam Apoptosis Antibody Array membrane. Antibody spots exhibiting signal differences are indicated in red boxes.

Using tissue or cell lysate samples may mask the positive control spots. This would need additional optimization steps.

Array - Human Apoptosis Antibody Array - Membrane (43 Targets) (AB134001)
  • Array

Supplier Data

Array - Human Apoptosis Antibody Array - Membrane (43 Targets) (AB134001)

Array using human serum, tested at a 5x dilution

Key facts

Reacts with

Human

Target

BAD

target

Applications

Multiplex Protein Detection

applications

Sample types

Cell culture supernatant, Plasma, Serum

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Multiplex Protein Detection": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Product details

ab134001 is for simultaneous detection of 43 Human Apoptotic markers.

Targets: Bad, Bax, Bcl-2, Bcl-w, BID, BIM, Caspase3, caspase8, CD40, CD40L, cIAP-2, cytoC, DR6, Fas, FasL, HSP27, HSP60, HSP70, HTRA, IGF-I, IGF-II, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-5, IGFBP-6, IGF-1sR, livin, p21, p27, p53, SMAC, Survivin, sTNF-R1, sTNF-R2, TNF-alpha, TNF-beta, TRAILR-1, TRAILR-2, TRAILR-3, TRAILR-4, XIAP

Caspase 3-will detect only the active form

Caspase 8-will detect the full length pro-form and the active form.

Antibody arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.

We do not recommend cell and tissue lysates for the ab134001 as it may mask the positive control spots.

Using tissue or cell lysate samples may mask the positive control spots. This would need additional optimization steps.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Product protocols

Target data

Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
See full target information BAD

Additional targets

BAX,BCL2,BCL2L2,BID,BCL2L11,CASP3,CASP8,CD40,CD40LG,BIRC3,CYCS,TNFRSF21,FAS,FASLG,HSPB1,HSPD1,HSPA4,HTRA2,IGF1,IGF1R,IGF2,IGFBP1,IGFBP2,IGFBP3,IGFBP4,IGFBP5,IGFBP6,BIRC7,CDKN1A,CDKN1B,TP53,DIABLO,BIRC5,TNF,LTA,TNFRSF1A,TNFRSF1B,TNFRSF10A,TNFRSF10B,TNFRSF10C,TNFRSF10D,XIAP,HSPA1B,HSPA1A

Publications (75)

Recent publications for all applications. Explore the full list and refine your search

Investigational new drugs 43:167-179 PubMed39875774

2025

Targeting MYC for the treatment of breast cancer: use of the novel MYC-GSPT1 degrader, GT19630.

Applications

Unspecified application

Species

Unspecified reactive species

Minhong Tang,John Crown,Michael J Duffy

Stem cell research & therapy 15:363 PubMed39396003

2024

Extracellular vesicles from human cardiac stromal cells up-regulate cardiomyocyte protective responses to hypoxia.

Applications

Unspecified application

Species

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Andreas Czosseck,Max M Chen,Chuan-Chih Hsu,Gleb Shamrin,Annette Meeson,Rachel Oldershaw,Helen Nguyen,Dora Livkisa,David J Lundy

RSC medicinal chemistry : PubMed39263684

2024

Exploring apoptotic induction of malabaricone A in triple-negative breast cancer cells: an acylphenol phyto-entity isolated from the fruit rind of Lam.

Applications

Unspecified application

Species

Unspecified reactive species

Pothiyil S Vimalkumar,Neethu Sivadas,Vishnu Priya Murali,Daisy R Sherin,Madhukrishnan Murali,Anuja Gracy Joseph,Kokkuvayil Vasu Radhakrishnan,Kaustabh Kumar Maiti

Stem cell research & therapy 15:189 PubMed38956646

2024

Autophagy modulation effect on homotypic transfer of intracellular components via tunneling nanotubes in mesenchymal stem cells.

Applications

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Species

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Fatemeh Sadeghsoltani,Çığır Biray Avci,Parisa Hassanpour,Sanya Haiaty,Mohamad Rahmati,Ali Mota,Reza Rahbarghazi,Maryam Nemati,Mahdi Mahdipour,Mehdi Talebi,Leila Sabour Takanlou,Maryam Sabour Takanlou,Amir Mehdizadeh

Cancer gene therapy 31:1164-1176 PubMed38918490

2024

Extracellular vesicles from type-2 macrophages increase the survival of chronic lymphocytic leukemia cells ex vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Léa Ikhlef,Nina Ratti,Stéphanie Durand,Rémy Formento,Héloïse Daverat,Marie Boutaud,Clément Guillou,Natalya Dmytruk,Nathalie Gachard,Pascal Cosette,Marie-Odile Jauberteau,Paul-François Gallet

Cancer cell international 24:207 PubMed38867223

2024

TEX19 increases the levels of CDK4 and promotes breast cancer by disrupting SKP2-mediated CDK4 ubiquitination.

Applications

Unspecified application

Species

Unspecified reactive species

Huantao Liu,He Wang,Hongyu Zhang,Miaomiao Yu,Yu Tang

Molecules (Basel, Switzerland) 29: PubMed38792269

2024

Anticancer Activity and Molecular Mechanisms of Acetylated and Methylated Quercetin in Human Breast Cancer Cells.

Applications

Unspecified application

Species

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Kozue Sakao,Shihomi Hamamoto,Daigo Urakawa,Ziyu He,De-Xing Hou

Cell death & disease 15:255 PubMed38600086

2024

Combination of bazedoxifene with chemotherapy and SMAC-mimetics for the treatment of colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Rhynelle S Dmello,Michelle Palmieri,Pathum S Thilakasiri,Larissa Doughty,Tracy L Nero,Ashleigh R Poh,Sarah Q To,Erinna F Lee,W Douglas Fairlie,Lisa Mielke,Michael W Parker,Ivan K H Poon,Eduard Batlle,Matthias Ernst,Ashwini L Chand

Biomolecules & biomedicine 24:323-336 PubMed37540586

2024

CCNI2 promotes pancreatic cancer through PI3K/AKT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Bingyang Hu,Wenzhi Zhang,Changsheng Zhang,Chonghui Li,Ning Zhang,Ke Pan,Xinlan Ge,Tao Wan

ChemMedChem 19:e202300680 PubMed38323458

2024

Effective Anticancer Potential of a New Sulfonamide as a Carbonic Anhydrase IX Inhibitor Against Aggressive Tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Ismail Koyuncu,Ebru Temiz,Eray Metin Güler,Mustafa Durgun,Ozgür Yuksekdag,Simone Giovannuzzi,Claudiu T Supuran
View all publications
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