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AB197424

Human Bone Metabolism Antibody Array (10 Targets) - Quantitative

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ab197424 can be used for quantitative measurement of 31 Human Bone Metabolism proteins.

View Alternative Names

BMP2A, BMP2, Bone morphogenetic protein 2, BMP-2, Bone morphogenetic protein 2A, BMP-2A, PDGF2, SIS, PDGFB, Platelet-derived growth factor subunit B, PDGF subunit B, PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis, VGR, BMP6, Bone morphogenetic protein 6, BMP-6, VG-1-related protein, VG-1-R, VGR-1, Transforming growth factor beta-3 proprotein, TGFB3, STMY1, MMP3, Stromelysin-1, SL-1, Matrix metalloproteinase-3, Transin-1, MMP-3, OP1, BMP7, Bone morphogenetic protein 7, BMP-7, Osteogenic protein 1, OP-1, OCIF, OPG, TNFRSF11B, Tumor necrosis factor receptor superfamily member 11B, Osteoclastogenesis inhibitory factor, Osteoprotegerin, CD254, OPGL, RANKL, TRANCE, TNFSF11, Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, Osteoprotegerin ligand, Receptor activator of nuclear factor kappa-B ligand, TNF-related activation-induced cytokine, ODF, BNSP, OPN, PSEC0156, SPP1, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin, SPP-1, UNQ492/PRO1008, DKK1, Dickkopf-related protein 1, Dickkopf-1, Dkk-1, hDkk-1, SK

2 Images
Multiplex Protein Detection - Human Bone Metabolism Antibody Array (10 Targets) - Quantitative (AB197424)
  • Multiplex Protein Detection

Supplier Data

Multiplex Protein Detection - Human Bone Metabolism Antibody Array (10 Targets) - Quantitative (AB197424)

Array Map for Abcam Human Bone Metabolism Antibody Array (10 Targets) - Quantitative (ab197424).

Multiplex Protein Detection - Human Bone Metabolism Antibody Array (10 Targets) - Quantitative (AB197424)
  • Multiplex Protein Detection

Supplier Data

Multiplex Protein Detection - Human Bone Metabolism Antibody Array (10 Targets) - Quantitative (AB197424)

Standard Curves obtained using Abcam Human Bone Metabolism Antibody Array (10 Targets) - Quantitative (ab197424).

Key facts

Reacts with

Human

Target

BMP2

target

Detection method

Fluorescent

Assay Platform

Multiplex

Sample types

Tissue Lysate, Plasma, Cell culture media, Serum, Other biological fluids, Cell Lysate

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Product details

ab197424 can be used for quantitative measurement of 31 Human Bone Metabolism proteins. Suitable serum, plasma, cell culture media, other body fluids, cell and tissue lysates.
Targets: BMP-2, BMP-6, BMP-7, DKK-1, MMP-3, OPG, OPN, PDGF-BB, TGFβ3, TRANCE.

Quantitative antibody arrays can be used to quantitate up to 40 cytokines with as little as 50 µL of sample. Arrays are available for 400 human and 200 mouse proteins. Each glass slide is spotted with 16 identical antibody arrays and is provided with a 16 well gasket to allow separate samples to be applied to each array. Each antibody is spotted in quadruplicate in each array. For reproducible quantitation, eight of the arrays are used with a cocktail of protein standards to produce a standard curve. The same 8 standard curve arrays can be used across multiple slides, allowing measurement of 22 experimental samples with 2 slides, 36 with 3 slides etc. For high through-put, 4 slides can be nested into a tray matching a standard microplate, allowing automated processing with a liquid handling workstation. Array processing can be completed within one working day (see workflow diagram below) and arrays can be analyzed with a wide number of laser glass slide array / gene microarray scanners. If you don’t have a suitable scanner then we recommend our membrane antibody arrays, which can be analyzed with any WB chemiluminescent reader. The standard is affinity purified. "

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes, including cardiogenesis, neurogenesis, and osteogenesis (PubMed : 18436533, PubMed : 24362451, PubMed : 31019025). Induces cartilage and bone formation (PubMed : 3201241). Initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2 (PubMed : 15064755, PubMed : 17295905, PubMed : 18436533). Once all three components are bound together in a complex at the cell surface, BMPR2 phosphorylates and activates BMPR1A (PubMed : 7791754). In turn, BMPR1A propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes. Also acts to promote expression of HAMP, via the interaction with its receptor BMPR1A/ALK3 (PubMed : 31800957). Can also signal through non-canonical pathways such as ERK/MAP kinase signaling cascade that regulates osteoblast differentiation (PubMed : 16771708, PubMed : 20851880). Also stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A-ATF4 pathway by stimulating EIF2A phosphorylation which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation (PubMed : 24362451). Acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, expression is repressed during the bell stage by MSX1-mediated inhibition of CTNNB1 signaling (By similarity).
See full target information BMP2

Additional targets

BMP6,BMP7,DKK1,MMP3,PDGFB,SPP1,TGFB3,TNFRSF11B,TNFSF11
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Product promise

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For full details, please see our Terms & Conditions

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