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AB197435

Human Chemokine Antibody Array (40 Targets) - Quantitative

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ab197435 can be used for quantitative measurement of 40 Human cytokines.

View Alternative Names

SCYA21, UNQ784/PRO1600, CCL21, C-C motif chemokine 21, 6Ckine, Beta-chemokine exodus-2, Secondary lymphoid-tissue chemokine, Small-inducible cytokine A21, SLC

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Multiplex Protein Detection - Human Chemokine Antibody Array (40 Targets) - Quantitative (AB197435)
  • Multiplex Protein Detection

Supplier Data

Multiplex Protein Detection - Human Chemokine Antibody Array (40 Targets) - Quantitative (AB197435)

Array map for Abcam Human Chemokine Antibody Array (40 Targets) - Quantitative (ab197435).

Multiplex Protein Detection - Human Chemokine Antibody Array (40 Targets) - Quantitative (AB197435)
  • Multiplex Protein Detection

Supplier Data

Multiplex Protein Detection - Human Chemokine Antibody Array (40 Targets) - Quantitative (AB197435)

Standard Curves obtained using Abcam Human Chemokine Antibody Array (40 Targets) - Quantitative (ab197435).

Multiplex Protein Detection - Human Chemokine Antibody Array (40 Targets) - Quantitative (AB197435)
  • Multiplex Protein Detection

Supplier Data

Multiplex Protein Detection - Human Chemokine Antibody Array (40 Targets) - Quantitative (AB197435)

Assay summary for Abcam Human Chemokine Antibody Array (40 Targets) - Quantitative (ab197435).

Key facts

Assay type

Quantitative

Reacts with

Human

Target

CCL21

target

Detection method

Fluorescent

Assay Platform

Multiplex

Sample types

Tissue Lysate, Plasma, Cell culture media, Serum, Other biological fluids, Cell Lysate

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Product details

ab197435 can be used for quantitative measurement of 40 Human cytokines. Suitable for serum, plasma, cell culture media, other body fluids, cell and tissue lysates.

Target names: 6Ckine, Axl, BTC, CCL28, CTACK, CXCL16, ENA-78, Eotaxin-3, GCP-2, GRO, HCC-1, HCC-4, IL-9, IL-17F, IL-18 BPa, IL-28A, IL-29, IL-31, IP-10, I-TAC, LIF, LIGHT, Lymphotactin, MCP-2, MCP-3, MCP-4, MDC, MIF, MIP-3α, MIP-3β, MPIF-1, MSPα, NAP-2, OPN, PARC, PF4, SDF-1α, TARC, TECK, TSLP.

The analytes measured by this kit are split across a single array on the same slide, provided in the kit.

Quantitative antibody arrays can be used to quantitate up to 40 cytokines with as little as 50 µL of sample. Arrays are available for 400 human and 200 mouse proteins. Each glass slide is spotted with 16 identical antibody arrays and is provided with a 16 well gasket to allow separate samples to be applied to each array.Each antibody is spotted in quadruplicate on each array. For reproducible quantitation, eight of the arrays are used with a cocktail of protein standards to produce a standard curve. The same 8 standard curve arrays can be used across multiple slides, allowing measurement of 22 experimental samples with 2 slides, 36 with 3 slides etc. For high through-put, 4 slides can be nested into a tray matching a standard microplate (not supplied), allowing automated processing with a liquid handling workstation. Array processing can be completed within one working day and arrays can be analyzed with a wide number of laser glass slide array / gene microarray scanners (see scanner requirements at ). If you don’t have a suitable scanner then we recommend our membrane antibody arrays, which can be analyzed with any WB chemiluminescent reader. See below for a schematic protocol, the fluorophore is a dye with equivalent to excitation/ emission to Cy3. The standard is affinity purified.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.
See full target information CCL21

Additional targets

AXL,Probetacellulin,CCL28,CCL27,CXCL16,CXCL5,CCL26,CXCL6,CXCL3,CCL14,CCL16,IL17F,Interleukin-18-binding protein,IFNL2,IFNL1,IL31,IL9,CXCL10,CXCL11,LIF,TNFSF14,XCL1,CCL8,CCL7,CCL13,CCL22,MIF,CCL20,CCL19,CCL23,MST1,PPBP,SPP1,CCL18,PF4,CXCL12,CCL17,CCL25,TSLP
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