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ab169812 is for the simultaneous detection 38 Human Chemokine Factors.

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Images

Multiplex Protein Detection - Human Chemokine Antibody Array - Membrane (38 targets) (AB169812), expandable thumbnail

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Key facts

Assay type
Semi-quantitative
Reactive species
Human
Target
CXCL13
(See target data below)

Target data

Function

Chemotactic for B-lymphocytes but not for T-lymphocytes, monocytes and neutrophils. Does not induce calcium release in B-lymphocytes. Binds to BLR1/CXCR5.

Additional Targets

CCL28, CCL23, CCL27, CXCL16, CXCL5, CCL11, CCL24, CCL26, CX3CL1, CXCL6, CXCL1, CXCL3, CCL16, CCL1, CXCL8, CXCL10, CXCL11, XCL1, CCL2, CCL8, CCL7, CCL13, CCL22, CXCL9, CCL3, CCL4, CCL15, CCL20, CCL19, CCL23, PPBP, CCL18, CCL5, CXCL12, CXCL12, CCL17, CCL25

Alternative names

BCA1, BLC, SCYB13, CXCL13, C-X-C motif chemokine 13, Angie, B cell-attracting chemokine 1, B lymphocyte chemoattractant, CXC chemokine BLC, Small-inducible cytokine B13, BCA-1

What's included?

1 x 8 Membranes
Components
1,000X HRP-Conjugated Streptavidin
1 x 50 µL
1X Blocking Buffer
2 x 25 mL
2000X Biotin-Conjugated Anti Cytokines
4 x 1 Vial
20X Wash Buffer I
1 x 20 mL
20X Wash Buffer II
1 x 20 mL
2X Cell Lysis Buffer
1 x 16 mL
8-Well Incubation Tray (with Lid)
1 x 1 Unit
Chemokine Antibody Array Membranes
2 x 4 Unit
Detection Buffer C
1 x 2.5 mL
Detection Buffer D
1 x 2.5 mL
Plastic sheets
1 x 1 Unit

Recommended products

ab169812 is for the simultaneous detection 38 Human Chemokine Factors.

Key facts

Assay type
Semi-quantitative
Reactive species
Human
Target
CXCL13
Applications
Multiplex Protein Detection
Sample types
Saliva, Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture media, Cell culture supernatant, Milk, Serum, Other biological fluids, Whole Blood, Cell Lysate

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

ab169812 is for the simultaneous detection 38 Human Chemokine Factors. Suitable for all liquid sample types.

Targets: BLC / CXCL13, CCL28, Ck beta 8-1 / CCL23, CTACK, CXCL16, ENA-78 / CXCL5, Eotaxin-1 / CCL11, Eotaxin-2 / CCL24, Eotaxin-3 / CCL26, Fractalkine / CX3CL1, GCP-2 / CXCL6, GRO / CXCL1+2+3, GRO alpha / CXCL1, HCC-4 / CCL16, I-309 / CCL1, I-TAC / CXCL11, IL-8 / CXCL8, IP-10 / CXCL10, Lymphotactin / XCL1, MCP-1 / CCL2, MCP-2 / CCL8, MCP-3 / CCL7, MCP-4 / CCL13, MDC / CCL22, MIG / CXCL9, MIP-1 alpha / CCL3, MIP-1 beta / CCL4, MIP-1 delta / CCL15, MIP-3 alpha / CCL20, MIP-3 beta / CCL19, MPIF-1 / CCL23, NAP-2 / CXCL7 / PPBP, PARC / CCL18, RANTES / CCL5, SDF-1 alpha / CXCL12alpha, SDF-1 beta / CXCL12beta, TARC / CCL17, TECK / CCL25

Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.


If you are interested in this cytokine array, array ab133998 may also be of interest. **A table listing all of our human membrane antibody cytokine arrays and other arrays and the analytes they measure is available .** "


Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Multiplex Protein Detection - Human Chemokine Antibody Array - Membrane (38 targets) (ab169812), expandable thumbnail

    Multiplex Protein Detection - Human Chemokine Antibody Array - Membrane (38 targets) (ab169812)

    Typical images obtained with Abcam Human Cytokine Antibody Array. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at room temperature for 1 minute.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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