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Abcam's Human Cytokine Antibody Array (120 Targets)- Quantitative ab197426 can be used for quantitative measurement of 120 Human Cytokine proteins.

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Multiplex Protein Detection - Human Cytokine Antibody Array (120 Targets) - Quantitative (AB197426), expandable thumbnail

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Key facts

Assay type

Quantitative

Reactive species

Human

Target

CCL21
(See target data below)

Assay Platform

Multiplex

Target data

Function

Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.

Targets

AREG, AXL, BDNF, BTC, NGF, FGF2, CXCL13, BMP4, BMP5, BMP7, CCL28, CCL27, CXCL16, CXCL5, EGF, EGFR, PROK1, CCL11, CCL24, CCL26, FGF4, FGF7, CXCL6, CSF3, GDF15, GDNF, CSF2, CXCL3, GH1, HBEGF, CCL14, CCL16, HGF, CCL1, ICAM1, IFNG, IGF1, IGFBP1, IGFBP2, IGFBP3, IGFBP4, IGFBP6, IL1A, IL1B, IL1RN, IL10, IL11, IL12B, IL12A, IL13, IL15, IL16, IL17A, IL17F, IL18BP, IL2, IFNL2, IFNL1, IL31, IL4, IL5, IL6, IL6R, IL7, CXCL8, IL9, INS, CXCL10, CXCL11, LIF, TNFSF14, XCL1_HUMAN, CCL2, CCL8, CCL7, CCL13, CSF1, CSF1R, CCL22, MIF, CXCL9, CCL3, CCL4, CCL15, CCL20, CCL19, CCL23, MST1, PPBP, NGFR, NTF3, NTF4, SPP1, TNFRSF11B, CCL18, PDGFA, PDGFB, PF4, PGF, CCL5, KITLG, KIT, CXCL12, CCL17, CCL25, TGFA, TGFB1, TGFB3, TIMP1, TIMP2, TNF, LTA, TNFRSF1A, TNFRSF1B, TSLP, VEGFA, VEGFD, KDR, FLT4

Alternative names

What's included?

50 Test
Components
20X Wash Buffer I
18 x 30 mL
20X Wash Buffer II
6 x 30 mL
Adhesive Film
12 x 1 Unit
Cy3 equivalent dye-conjugated Streptavidin
12 x 5 µL
Human Chemokine Array (40 T) Biotinylated Antibody Cocktail
4 x 25 µL
Human Chemokine Array (40 T) Glass Slide
4 x 1 Slide
Human Chemokine Array (40 T) Lyophilized Cytokine Standard Mix
2 x 1 Vial
Human Growth Factor Array (40 T) Biotinylated Antibody Cocktail
4 x 25 µL
Human Growth Factor Array (40 T) Glass Slide
4 x 1 Slide
Human Growth Factor Array (40 T) Lyophilized Cytokine Standard Mix
2 x 1 Vial
Human Inflammation Array (40 T) Biotinylated Antibody Cocktail
4 x 25 µL
Human Inflammation Array (40 T) Glass Slide
4 x 1 Slide
Human Inflammation Array (40 T) Lyophilized Cytokine Standard Mix
2 x 1 Vial
Sample Diluent
6 x 15 mL
Slide Washer/Dryer (30 mL Centrifuge Tube)
6 x 1 Unit

Recommended products

Abcam's Human Cytokine Antibody Array (120 Targets)- Quantitative ab197426 can be used for quantitative measurement of 120 Human Cytokine proteins.

Key facts

Assay type

Quantitative

Reactive species

Human

Target

CCL21

Detection method

Fluorescent

Assay Platform

Multiplex

Sample types

Tissue Lysate, Plasma, Cell culture media, Serum, Cell Lysate

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Abcam's Human Cytokine Antibody Array (120 Targets)- Quantitative ab197426 can be used for quantitative measurement of 120 Human Cytokine proteins. Suitable for serum, plasma, cell culture media, other body fluids, cell and tissue lysates.
Targets: 6Ckine (CCL21), Amphiregulin, Axl, BDNF, Betacellulin (BTC), beta-NGF, bFGF, BLC (CXCL13), BMP-4, BMP-5, BMP-7, CCL28 (MEC), CTACK (CCL27), CXCL16, EGF, EGFR, EG-VEGF (PK1), ENA-78 (CXCL5), Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), Eotaxin-3 (CCL26), FGF-4, FGF-7 (KGF), GCP-2 (CXCL6), GCSF, GDF-15, GDNF, GM-CSF, GRO alpha/beta/gamma, Growth Hormone, HB-EGF, HCC-1 (CCL14), HCC-4 (CCL16), HGF, I-309 (TCA-3/CCL1), ICAM-1 (CD54), IFN-gamma, IGF-1, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-1 ra (IL-1 F3), IL-10, IL-11, IL-12 p40, IL-12 p70, IL-13, IL-15, IL-16, IL-17A, IL-17F, IL-18 BP alpha, IL-2, IL-28A (IFN-lambda 2), IL-29 (IFN-lambda 1), IL-31, IL-4, IL-5, IL-6, IL-6 R, IL-7, IL-8 (CXCL8), IL-9, Insulin, IP-10 (CXCL10), I-TAC (CXCL11), LIF, LIGHT (TNFSF14), Lymphotactin (XCL1), MCP-1 (CCL2), MCP-2 (CCL8), MCP-3 (MARC/CCL7), MCP-4 (CCL13), M-CSF, M-CSF R, MDC (CCL22), MIF, MIG (CXCL9), MIP-1 alpha (CCL3), MIP-1 beta (CCL4), MIP-1 delta (CCL15), MIP-3 alpha (CCL20), MIP-3 beta (CCL19), MPIF-1 (CCL23), MSP alpha/beta, NAP-2 (PPBP/CXCL7), NGFR (TNFRSF16), NT-3, NT-4, Osteopontin (SPP1), Osteoprotegerin (TNFRSF11B), PARC (CCL18), PDGF-AA, PDGF-BB, Platelet Factor 4 (CXCL4), PLGF, RANTES (CCL5), SCF, SCF R (CD117/c-kit), SDF-1 alpha (CXCL12 alpha), TARC (CCL17), TECK (CCL25), TGF alpha, TGF beta 1, TGF beta 3, TIMP-1, TIMP-2, TNF alpha, TNF beta (TNFSF1B), TNF RI (TNFRSF1A), TNF RII (TNFRSF1B), TSLP, VEGF-A, VEGF-D, VEGFR2, VEGFR3.
Quantitative antibody arrays can be used to quantitate up to 40 cytokines with as little as 50 µL of sample. Arrays are available for 400 human and 200 mouse proteins. Each glass slide is spotted with 16 identical antibody arrays and is provided with a 16 well gasket to allow separate samples to be applied to each array. Each antibody is spotted in quadruplicate in each array. For reproducible quantitation, eight of the arrays are used with a cocktail of protein standards to produce a standard curve. The same 8 standard curve arrays can be used across multiple slides, allowing measurement of 22 experimental samples with 2 slides, 36 with 3 slides etc. For high through-put, 4 slides can be nested into a tray matching a standard microplate, allowing automated processing with a liquid handling workstation. Array processing can be completed within one working day (see workflow diagram below) and arrays can be analyzed with a wide number of laser glass slide array / gene microarray scanners. If you don’t have a suitable scanner then we recommend our membrane antibody arrays, which can be analyzed with any WB chemiluminescent reader. The standard is affinity purified. "

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  • Multiplex Protein Detection - Human Cytokine Antibody Array (120 Targets) - Quantitative (ab197426), expandable thumbnail

    Multiplex Protein Detection - Human Cytokine Antibody Array (120 Targets) - Quantitative (ab197426)

    Assay summary for Abcam Human Cytokine Antibody Array (120 Targets) - Quantitative (ab197426).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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