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AB197425

Human Cytokine Antibody Array (80 Targets) - Quantitative

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ab197425 can be used for quantitative measurement of 80 Human cytokines.

View Alternative Names

AREGB, SDGF, AREG, Amphiregulin, AR, Colorectum cell-derived growth factor, CRDGF

Key facts

Assay type

Quantitative

Reacts with

Human

Target

AREG

target

Detection method

Fluorescent

Assay Platform

Multiplex

Sample types

Tissue Lysate, Plasma, Cell culture supernatant, Serum, Other biological fluids, Cell Lysate

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Product details

ab197425 can be used for quantitative measurement of 80 Human cytokines. Suitable for serum, plasma, cell culture media, other body fluid, cell and tissue lysates.
Targets: BLC, Eotaxin, Eotaxin-2, G-CSF, GM-CSF, I-309, ICAM-1, IFNγ, IL-1α, IL-1β, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-6 sR, IL-7, IL-8, IL-10, IL-11, IL-12p40, IL-12p70, IL-13, IL-15, IL-16, IL-17, MCP-1, M-CSF, MIG, MIP-1α, MIP-1β, MIP-1δ, PDGF-BB, RANTES, TIMP-1, TIMP-2, TNFα, TNFβ, TNF sRI, TNF sRII, AR, BDNF, bFGF, BMP-4, BMP-5, BMP-7, β-NGF, EGF, EGF R, EG-VEGF, FGF-4, FGF-7, GDF-15, GDNF, GH, HB-EGF, HGF, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IGF-I, Insulin, MCSF R, NGF R, NT-3, NT-4, OPG, PDGF-AA, PIGF, SCF, SCF R, TGFα, TGFβ1, TGFβ3, VEGF, VEGF R2, VEGF R3, VEGF-D.

The analytes measured by this kit are split across 2 different arrays on different slides, both of which are provided in the kit.

Quantitative antibody arrays can be used to quantitate up to 40 cytokines with as little as 50 µL of sample. Arrays are available for 400 human and 200 mouse proteins. Each glass slide is spotted with 16 identical antibody arrays and is provided with a 16 well gasket to allow separate samples to be applied to each array. Each antibody is spotted in quadruplicate in each array. For reproducible quantitation, eight of the arrays are used with a cocktail of protein standards to produce a standard curve. The same 8 standard curve arrays can be used across multiple slides, allowing measurement of 22 experimental samples with 2 slides, 36 with 3 slides etc. For high through-put, 4 slides can be nested into a tray matching a standard microplate, allowing automated processing with a liquid handling workstation. Array processing can be completed within one working day (see workflow diagram below) and arrays can be analyzed with a wide number of laser glass slide array / gene microarray scanners. If you don’t have a suitable scanner then we recommend our membrane antibody arrays, which can be analyzed with any WB chemiluminescent reader. The standard is affinity purified. . ."

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.
See full target information AREG

Additional targets

BDNF,NGF,FGF2,CXCL13,BMP4,Bone morphogenetic protein 5,BMP7,EGF,EGFR,PROK1,CCL11,CCL24,FGF4,FGF7,CSF3,GDF15,GDNF,CSF2,GH1,HBEGF,HGF,CCL1,ICAM1,IFNG,IGF1,IGFBP1,IGFBP2,IGFBP3,IGFBP4,IGFBP6,IL1A,IL1B,IL1RN,IL10,IL11,IL12B,IL12A,IL13,IL15,IL16,IL17A,IL2,IL4,IL5,IL6,IL6R,IL7,CXCL8,INS,CCL2,CSF1,CSF1R,CXCL9,CCL3,CCL4,CCL15,NGFR,NTF3,NTF4,TNFRSF11B,PDGFA,PDGFB,PGF,CCL5,KITLG,KIT,Protransforming growth factor alpha,TGFB1,TGFB3,TIMP1,TIMP2,TNF,LTA,TNFRSF1A,TNFRSF1B,VEGFA,VEGFD,KDR,FLT4
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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