JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB197436

Human Cytokine Antibody Array A (20 Targets) - Quantitative

Be the first to review this product! Submit a review

|

(0 Publication)

ab197436 can be used for quantitative measurement of 20 Human cytokines.
3 Images
Multiplex Protein Detection - Human Cytokine Antibody Array A (20 Targets) - Quantitative (AB197436)
  • Multiplex Protein Detection

Supplier Data

Multiplex Protein Detection - Human Cytokine Antibody Array A (20 Targets) - Quantitative (AB197436)

Standard Curve obtained with Abcam Human Cytokine Antibody Array A (20 Targets) - Quantitative (ab197436).

Multiplex Protein Detection - Human Cytokine Antibody Array A (20 Targets) - Quantitative (AB197436)
  • Multiplex Protein Detection

Supplier Data

Multiplex Protein Detection - Human Cytokine Antibody Array A (20 Targets) - Quantitative (AB197436)

Assay summary for Abcam Human Cytokine Antibody Array A (20 Targets) - Quantitative (ab197436).

Multiplex Protein Detection - Human Cytokine Antibody Array A (20 Targets) - Quantitative (AB197436)
  • Multiplex Protein Detection

Supplier Data

Multiplex Protein Detection - Human Cytokine Antibody Array A (20 Targets) - Quantitative (AB197436)

Array map for Abcam Human Cytokine Antibody Array A (20 Targets) - Quantitative (ab197436).

Key facts

Reacts with

Human

Target

CCL2

target

Detection method

Fluorescent

Assay Platform

Multiplex

Sample types

Tissue Lysate, Plasma, Cell culture media, Serum, Other biological fluids, Cell Lysate

style
left-column

Complementary Products

Reagents, Controls & Accessories

AB270055

Protease Inhibitor Cocktail EDTA Free (100X)

  • 0
  • 0
Multiplex Kits

AB133999

Mouse Inflammation Antibody Array - Membrane (40 Targets)

Multiplex Protein Detection - Mouse Inflammation Antibody Array - Membrane (40 Targets) (AB133999)

  • 0
  • 0
Multiplex Kits

AB133998

Cytokine Array - Human Cytokine Antibody Array (Membrane, 80 Targets)

Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 80 Targets) (AB133998)

  • 0
  • 0
Assay Kits

AB210966

Human Pro-Collagen I alpha 1 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (AB210966)

  • 5
  • 1
Multiplex Kits

AB134002

Human Growth Factor Antibody Array - Membrane (41 Targets)

Multiplex Protein Detection - Human Growth Factor Antibody Array - Membrane (41 Targets) (AB134002)

  • 0
  • 0
style
left-column

Product details

ab197436 can be used for quantitative measurement of 20 Human cytokines. Suitable for serum, plasma, cell culture media, other body fluids, cell and tissue lysates.

Target names: GRO, GM-CSF, IFNγ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12, IL-13, MCP-1, MIP-1α, MIP-1β, MMP-9, RANTES, TNFα, VEGF.

The analytes measured by this kit are split across a single array on the same slide, provided in the kit.

Quantitative antibody arrays can be used to quantitate up to 40 cytokines with as little as 50 µL of sample. Arrays are available for 400 human and 200 mouse proteins. Each glass slide is spotted with 16 identical antibody arrays and is provided with a 16 well gasket to allow separate samples to be applied to each array. Each antibody is spotted in quadruplicate in each array. For reproducible quantitation, eight of the arrays are used with a cocktail of protein standards to produce a standard curve. The same 8 standard curve arrays can be used across multiple slides, allowing measurement of 22 experimental samples with 2 slides, 36 with 3 slides etc. For high through-put, 4 slides can be nested into a tray matching a standard microplate, allowing automated processing with a liquid handling workstation. Array processing can be completed within one working day (see workflow diagram below) and arrays can be analyzed with a wide number of laser glass slide array / gene microarray scanners. If you don’t have a suitable scanner then we recommend our membrane antibody arrays, which can be analyzed with any WB chemiluminescent reader. The standard is affinity purified.

style
left-column

What's included?

{ "values": { "50Test": { "sellingSize": "50 Test", "publicAssetCode":"ab197436-50Test", "assetComponentDetails": [ { "size":"6 x 30 mL", "name":"20X Wash Buffer I", "number":"AB197436-CMP06" }, { "size":"2 x 30 mL", "name":"20X Wash Buffer II", "number":"AB197436-CMP07" }, { "size":"2 x 1 Unit", "name":"Slide Washer/Dryer (30 mL Centrifuge Tube)", "number":"AB197436-CMP02" }, { "size":"2 x 15 mL", "name":"Sample Diluent", "number":"AB197436-CMP01" }, { "size":"4 x 1 Slide", "name":"Human Cytokine Array A (20 T) Glass Slide", "number":"AB197436-CMP04" }, { "size":"2 x 1 Vial", "name":"Human Cytokine Array A (20 T) Lyophilized Cytokine Standard Mix", "number":"AB197436-CMP05" }, { "size":"4 x 25 µL", "name":"Human Cytokine Array A (20 T) Biotinylated Antibody Cocktail", "number":"AB197436-CMP03" }, { "size":"4 x 1 Unit", "name":"Adhesive Film", "number":"AB197436-CMP09" }, { "size":"4 x 5 µL", "name":"Cy3 equivalent dye-conjugated Streptavidin", "number":"AB197436-CMP08" } ] }, "8Test": { "sellingSize": "8 Test", "publicAssetCode":"ab197436-8Test", "assetComponentDetails": [ { "size":"1 x 1 Slide", "name":"Human Cytokine Array A (20 T) Glass Slide", "number":"AB197436-CMP04" }, { "size":"1 x 30 mL", "name":"20X Wash Buffer II", "number":"AB197436-CMP07" }, { "size":"1 x 1 Unit", "name":"Slide Washer/Dryer (30 mL Centrifuge Tube)", "number":"AB197436-CMP02" }, { "size":"1 x 15 mL", "name":"Sample Diluent", "number":"AB197436-CMP01" }, { "size":"2 x 30 mL", "name":"20X Wash Buffer I", "number":"AB197436-CMP06" }, { "size":"1 x 25 µL", "name":"Human Cytokine Array A (20 T) Biotinylated Antibody Cocktail", "number":"AB197436-CMP03" }, { "size":"1 x 1 Vial", "name":"Human Cytokine Array A (20 T) Lyophilized Cytokine Standard Mix", "number":"AB197436-CMP05" }, { "size":"1 x 1 Unit", "name":"Adhesive Film", "number":"AB197436-CMP09" }, { "size":"1 x 5 µL", "name":"Cy3 equivalent dye-conjugated Streptavidin", "number":"AB197436-CMP08" } ] }, "22Test": { "sellingSize": "22 Test", "publicAssetCode":"ab197436-22Test", "assetComponentDetails": [ { "size":"3 x 30 mL", "name":"20X Wash Buffer I", "number":"AB197436-CMP06", "productcode":"" }, { "size":"1 x 30 mL", "name":"20X Wash Buffer II", "number":"AB197436-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Slide Washer/Dryer (30 mL Centrifuge Tube)", "number":"AB197436-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"Sample Diluent", "number":"AB197436-CMP01", "productcode":"" }, { "size":"2 x 1 Slide", "name":"Human Cytokine Array A (20 T) Glass Slide", "number":"AB197436-CMP04", "productcode":"" }, { "size":"2 x 25 µL", "name":"Human Cytokine Array A (20 T) Biotinylated Antibody Cocktail", "number":"AB197436-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Human Cytokine Array A (20 T) Lyophilized Cytokine Standard Mix", "number":"AB197436-CMP05", "productcode":"" }, { "size":"2 x 1 Unit", "name":"Adhesive Film", "number":"AB197436-CMP09", "productcode":"" }, { "size":"2 x 5 µL", "name":"Cy3 equivalent dye-conjugated Streptavidin", "number":"AB197436-CMP08", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

Acts as a ligand for C-C chemokine receptor CCR2 (PubMed : 10529171, PubMed : 10587439, PubMed : 9837883). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (PubMed : 10587439, PubMed : 9837883). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (PubMed : 8195247, PubMed : 8627182, PubMed : 9792674). May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis (PubMed : 8107690).
See full target information CCL2

Additional targets

CCL3,CCL4,CCL5,CSF2,CXCL1,CXCL2,CXCL3,CXCL8,IFNG,IL10,IL12A,IL13,IL1A,IL1B,IL2,IL4,IL5,IL6,TNF,VEGFA

Alternative Names

MCP1, SCYA2, CCL2, C-C motif chemokine 2, HC11, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, Small-inducible cytokine A2, MCAF, MCP-1, IFNB2, IL6, Interleukin-6, IL-6, B-cell stimulatory factor 2, CTL differentiation factor, Hybridoma growth factor, Interferon beta-2, BSF-2, CDF, IFN-beta-2, D17S136E, SCYA5, CCL5, C-C motif chemokine 5, EoCP, Eosinophil chemotactic cytokine, SIS-delta, Small-inducible cytokine A5, T cell-specific protein P228, T-cell-specific protein RANTES, TCP228, GRO2, GROB, MIP2A, SCYB2, CXCL2, C-X-C motif chemokine 2, Growth-regulated protein beta, Macrophage inflammatory protein 2-alpha, Gro-beta, MIP2-alpha, LAG1, MIP1B, SCYA4, CCL4, C-C motif chemokine 4, G-26 T-lymphocyte-secreted protein, HC21, Lymphocyte activation gene 1 protein, MIP-1-beta(1-69), Macrophage inflammatory protein 1-beta, PAT 744, Protein H400, SIS-gamma, Small-inducible cytokine A4, T-cell activation protein 2, LAG-1, MIP-1-beta, ACT-2, Interleukin-2, IL-2, T-cell growth factor, TCGF, IL2, Interleukin-4, IL-4, B-cell stimulatory factor 1, Binetrakin, Lymphocyte stimulatory factor 1, Pitrakinra, BSF-1, IL4, IL1F2, IL1B, Interleukin-1 beta, IL-1 beta, Catabolin, TNFA, TNFSF2, TNF, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, G0S19-1, MIP1A, SCYA3, CCL3, C-C motif chemokine 3, G0/G1 switch regulatory protein 19-1, Macrophage inflammatory protein 1-alpha, PAT 464.1, SIS-beta, Small-inducible cytokine A3, Tonsillar lymphocyte LD78 alpha protein, MIP-1-alpha, GMCSF, CSF2, Granulocyte-macrophage colony-stimulating factor, GM-CSF, Colony-stimulating factor, Molgramostin, Sargramostim, CSF, GRO, GRO1, GROA, MGSA, SCYB1, CXCL1, Growth-regulated alpha protein, C-X-C motif chemokine 1, GRO-alpha(1-73), Melanoma growth stimulatory activity, Neutrophil-activating protein 3, NAP-3, GRO3, GROG, SCYB3, CXCL3, C-X-C motif chemokine 3, GRO-gamma(1-73), Growth-regulated protein gamma, Macrophage inflammatory protein 2-beta, GRO-gamma, MIP2-beta, Interferon gamma, IFN-gamma, Immune interferon, IFNG, IL1F1, IL1A, Interleukin-1 alpha, IL-1 alpha, Hematopoietin-1, Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10, NC30, IL13, Interleukin-13, IL-13, Interleukin-5, IL-5, B-cell differentiation factor I, Eosinophil differentiation factor, T-cell replacing factor, TRF, IL5, IL8, CXCL8, Interleukin-8, IL-8, C-X-C motif chemokine 8, Chemokine (C-X-C motif) ligand 8, Emoctakin, Granulocyte chemotactic protein 1, Monocyte-derived neutrophil chemotactic factor, Monocyte-derived neutrophil-activating peptide, Neutrophil-activating protein 1, Protein 3-10C, T-cell chemotactic factor, GCP-1, MDNCF, MONAP, NAP-1, VEGF, VEGFA, L-VEGF, Vascular permeability factor, VPF, NKSF1, IL12A, Interleukin-12 subunit alpha, IL-12A, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, NK cell stimulatory factor chain 1, CLMF p35
style
left-column
style
left-column
websiteProtocolBooklet
en
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com