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AB197442

Human Cytokine Antibody Array E (40 Targets) - Quantitative

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ab197442 can be used for quantitative measurement of 40 Human cytokines.

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ANGPT5, UNQ153/PRO179, ANGPTL3, Angiopoietin-related protein 3, Angiopoietin-5, Angiopoietin-like protein 3, ANG-5, CD23, CD23A, CLEC4J, FCE2, IGEBF, FCER2, Low affinity immunoglobulin epsilon Fc receptor, BLAST-2, C-type lectin domain family 4 member J, Fc-epsilon-RII, Immunoglobulin E-binding factor, Lymphocyte IgE receptor, ZCYTO10, UNQ852/PRO1801, IL20, Interleukin-20, IL-20, Cytokine Zcyto10, FIL1Z, IL1F7, IL1H4, IL1RP1, IL37, Interleukin-37, IL-37, FIL1 zeta, IL-1X, Interleukin-1 family member 7, Interleukin-1 homolog 4, Interleukin-1 zeta, Interleukin-1-related protein, IL-1F7, IL-1H, IL-1H4, IL-1 zeta, IL-1RP1, Chitinase-3-like protein 1, 39 kDa synovial protein, Cartilage glycoprotein 39, YKL-40, CGP-39, GP-39, hCGP-39, CHI3L1, CDHP, CDH3, Cadherin-3, Placental cadherin, P-cadherin, MER, MERTK, Tyrosine-protein kinase Mer, Proto-oncogene c-Mer, Receptor tyrosine kinase MerTK, Fibroblast growth factor 9, FGF-9, Glia-activating factor, Heparin-binding growth factor 9, GAF, HBGF-9, FGF9, CD125, IL5R, IL5RA, Interleukin-5 receptor subunit alpha, IL-5 receptor subunit alpha, IL-5R subunit alpha, IL-5R-alpha, IL-5RA, CDw125, IL1E, IL1F9, IL1H1, IL1RP2, UNQ2456/PRO5737, IL36G, Interleukin-36 gamma, IL-1-related protein 2, Interleukin-1 epsilon, Interleukin-1 family member 9, Interleukin-1 homolog 1, IL-1RP2, IL-1 epsilon, IL-1F9, IL-1H1, CD152, CTLA4, Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4, CTLA-4, CPSB, CTSB, Cathepsin B, APP secretase, Cathepsin B1, APPS, CD26, ADCP2, DPP4, Dipeptidyl peptidase 4, ADABP, Adenosine deaminase complexing protein 2, Dipeptidyl peptidase IV, T-cell activation antigen CD26, TP103, ADCP-2, DPP IV, APO3L, DR3LG, UNQ181/PRO207, TNFSF12, Tumor necrosis factor ligand superfamily member 12, APO3 ligand, TNF-related weak inducer of apoptosis, TWEAK, FOLH, NAALAD1, PSM, PSMA, GIG27, FOLH1, Glutamate carboxypeptidase 2, Cell growth-inhibiting gene 27 protein, Folate hydrolase 1, Folylpoly-gamma-glutamate carboxypeptidase, Glutamate carboxypeptidase II, Membrane glutamate carboxypeptidase, N-acetylated-alpha-linked acidic dipeptidase I, Prostate-specific membrane antigen, Pteroylpoly-gamma-glutamate carboxypeptidase, FGCP, GCPII, mGCP, NAALADase I, UNQ561/PRO1122, IL17C, Interleukin-17C, IL-17C, Cytokine CX2, CD218a, IL1RRP, IL18R1, Interleukin-18 receptor 1, IL-18R-1, IL-18R1, CD218 antigen-like family member A, CDw218a, IL1 receptor-related protein, Interleukin-18 receptor alpha, IL-1Rrp, IL1R-rp, IL-18R-alpha, IL-18Ralpha, CD142, Tissue factor, TF, Coagulation factor III, Thromboplastin, F3, HST2, HSTF2, FGF6, Fibroblast growth factor 6, FGF-6, Heparin secretory-transforming protein 2, Heparin-binding growth factor 6, HST-2, HSTF-2, HBGF-6, UNQ208/PRO234, LAYN, Layilin, G250, MN, CA9, Carbonic anhydrase 9, Carbonate dehydratase IX, Carbonic anhydrase IX, Membrane antigen MN, P54/58N, Renal cell carcinoma-associated antigen G250, pMW1, CA-IX, CAIX, RCC-associated antigen G250, IGIF, IL1F4, IL18, Interleukin-18, IL-18, Iboctadekin, Interferon gamma-inducing factor, Interleukin-1 gamma, IFN-gamma-inducing factor, IL-1 gamma, FIL1T, IL1HY2, IL38, FKSG75, UNQ6119/PRO20041, IL1F10, Interleukin-1 family member 10, IL-1F10, Family of interleukin 1-theta, Interleukin-1 HY2, Interleukin-1 theta, Interleukin-38, FIL1 theta, IL-1HY2, IL-1 theta, IL-38, FIL1E, IL1E, IL1F6, IL36A, Interleukin-36 alpha, FIL1 epsilon, Interleukin-1 epsilon, Interleukin-1 family member 6, IL-1 epsilon, IL-1F6, FIL1D, IL1F5, IL1HY1, IL1L1, IL1RP3, UNQ1896/PRO4342, IL36RN, Interleukin-36 receptor antagonist protein, IL-36Ra, FIL1 delta, IL-1-related protein 3, Interleukin-1 HY1, Interleukin-1 delta, Interleukin-1 family member 5, Interleukin-1 receptor antagonist homolog 1, Interleukin-1-like protein 1, IL-1RP3, IL-1HY1, IL-1 delta, IL-1F5, IL-1ra homolog 1, IL-1L1, C16orf77, IL34, Interleukin-34, IL-34, ED1, EDA2, EDA, Ectodysplasin-A, Ectodermal dysplasia protein, EDA protein, CD295, DB, OBR, LEPR, Leptin receptor, LEP-R, HuB219, OB receptor, OB-R, BIGH3, TGFBI, Transforming growth factor-beta-induced protein ig-h3, Beta ig-h3, Kerato-epithelin, RGD-containing collagen-associated protein, RGD-CAP, Erythropoietin receptor, EPO-R, EPOR, CD210, IL10R, IL10RA, Interleukin-10 receptor subunit alpha, IL-10 receptor subunit alpha, IL-10R subunit alpha, IL-10RA, CDw210a, Interleukin-10 receptor subunit 1, IL-10R subunit 1, IL-10R1, Transforming growth factor beta receptor type 3, TGF-beta receptor type 3, TGFR-3, Betaglycan, Transforming growth factor beta receptor III, TGF-beta receptor type III, TGFBR3, Prostasin, Channel-activating protease 1, Serine protease 8, CAP1, PRSS8, Dickkopf-related protein 4, Dickkopf-4, Dkk-4, hDkk-4, DKK4, UNQ301/PRO342, SIGIRR, Single Ig IL-1-related receptor, Single Ig IL-1R-related molecule, Single immunoglobulin domain-containing IL1R-related protein, Toll/interleukin-1 receptor 8, TIR8, MPSL1, PUMP1, MMP7, Matrilysin, Matrin, Matrix metalloproteinase-7, Pump-1 protease, Uterine metalloproteinase, MMP-7, IL1F8, IL1H2, IL36B, Interleukin-36 beta, FIL1 eta, Interleukin-1 eta, Interleukin-1 family member 8, Interleukin-1 homolog 2, IL-1 eta, IL-1F8, IL-1H2, IBP5, IGFBP5, Insulin-like growth factor-binding protein 5, IBP-5, IGF-binding protein 5, IGFBP-5, MPN, UNQ1884/PRO4327, PRSS27, Serine protease 27, Marapsin, Pancreasin, Growth arrest-specific protein 1, GAS-1, GAS1

3 Images
Multiplex Protein Detection - Human Cytokine Antibody Array E (40 Targets) - Quantitative (AB197442)
  • Multiplex Protein Detection

Supplier Data

Multiplex Protein Detection - Human Cytokine Antibody Array E (40 Targets) - Quantitative (AB197442)

Array map for Abcam Human Cytokine Antibody Array E (40 Targets) - Quantitative (ab197442).

Multiplex Protein Detection - Human Cytokine Antibody Array E (40 Targets) - Quantitative (AB197442)
  • Multiplex Protein Detection

Supplier Data

Multiplex Protein Detection - Human Cytokine Antibody Array E (40 Targets) - Quantitative (AB197442)

Standard Curve obtained using Abcam Human Cytokine Antibody Array E (40 Targets) - Quantitative (ab197442).

Multiplex Protein Detection - Human Cytokine Antibody Array E (40 Targets) - Quantitative (AB197442)
  • Multiplex Protein Detection

Supplier Data

Multiplex Protein Detection - Human Cytokine Antibody Array E (40 Targets) - Quantitative (AB197442)

Assay summary for Abcam Human Cytokine Antibody Array E (40 Targets) - Quantitative (ab197442).

Key facts

Reacts with

Human

Target

ANGPTL3

target

Detection method

Fluorescent

Assay Platform

Multiplex

Sample types

Tissue Lysate, Plasma, Cell culture media, Serum, Other biological fluids, Cell Lysate

Product details

ab197442 can be used for quantitative measurement of 40 Human cytokines. Suitable for serum, plasma, cell culture media, other body fluids, cell and tissue lysates.

Targets names: ANGPTL3, βIG-H3, CA9, Cathepsin B, CD23, CHI3L1, CTLA4, Dkk-4, DPPIV, EDA-A2, Epo R, FGF-6, FGF-9, Gas1, IGFBP-5, IL-1F5, IL-1F6, IL-1F7, IL-1F8, IL-1F9, IL-1F10, IL-1R5, IL-17C, IL-18, IL-20, IL-34, IL-5 Rα, IL-10 Rα, Layilin, Leptin R, Marapsin, Mer, MMP-, P-Cadherin, Prostasin, PSMA, SIGIRR, TGFβ RIII, Tissue Factor, TWEAK.

Quantitative antibody arrays can be used to quantitate up to 40 cytokines with as little as 50 µL of sample. Arrays are available for 400 human and 200 mouse proteins. Each glass slide is spotted with 16 identical antibody arrays and is provided with a 16 well gasket to allow separate samples to be applied to each array. Each antibody is spotted in quadruplicate in each array. For reproducible quantitation, eight of the arrays are used with a cocktail of protein standards to produce a standard curve. The same 8 standard curve arrays can be used across multiple slides, allowing measurement of 22 experimental samples with 2 slides, 36 with 3 slides etc. For high through-put, 4 slides can be nested into a tray matching a standard microplate, allowing automated processing with a liquid handling workstation. Array processing can be completed within one working day (see workflow diagram below) and arrays can be analyzed with a wide number of laser glass slide array / gene microarray scanners. If you don’t have a suitable scanner then we recommend our membrane antibody arrays, which can be analyzed with any WB chemiluminescent reader. The standard is affinity purified.

What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Product protocols

Target data

Acts in part as a hepatokine that is involved in regulation of lipid and glucose metabolism (PubMed : 11788823, PubMed : 12909640, PubMed : 23661675, PubMed : 25495645). Proposed to play a role in the trafficking of energy substrates to either storage or oxidative tissues in response to food intake (By similarity). Has a stimulatory effect on plasma triglycerides (TG), which is achieved by suppressing plasma TG clearance via inhibition of LPL activity. The inhibition of LPL activity appears to be an indirect mechanism involving recruitment of proprotein convertases PCSK6 and FURIN to LPL leading to cleavage and dissociation of LPL from the cell surface; the function does not require ANGPTL3 proteolytic cleavage but seems to be mediated by the N-terminal domain, and is not inhibited by GPIHBP1 (PubMed : 12097324, PubMed : 19318355, PubMed : 20581395). Can inhibit endothelial lipase, causing increased plasma levels of high density lipoprotein (HDL) cholesterol and phospholipids (PubMed : 17110602, PubMed : 19028676). Can bind to adipocytes to activate lipolysis, releasing free fatty acids and glycerol (PubMed : 12565906). Suppresses LPL specifically in oxidative tissues which is required to route very low density lipoprotein (VLDL)-TG to white adipose tissue (WAT) for storage in response to food; the function may involve cooperation with circulating, liver-derived ANGPTL8 and ANGPTL4 expression in WAT (By similarity). Contributes to lower plasma levels of low density lipoprotein (LDL)-cholesterol by a mechanism that is independent of the canonical pathway implicating APOE and LDLR. May stimulate hypothalamic LPL activity (By similarity).. ANGPTL3(17-221). In vitro inhibits LPL activity; not effective on GPIHBP1-stabilized LPL.. Involved in angiogenesis. Binds to endothelial cells via integrin alpha-V/beta-3 (ITGAV : ITGB3), activates FAK, MAPK and Akt signaling pathways and induces cell adhesion and cell migration (PubMed : 11877390). Secreted from podocytes, may modulate properties of glomerular endothelial cells involving integrin alpha-V/beta-3 and Akt signaling (PubMed : 18535744). May increase the motility of podocytes. May induce actin filament rearrangements in podocytes implicating integrin alpha-V/beta-3 and Rac1 activation. Binds to hematopoietic stem cells (HSC) and is involved in the regulation of HSC activity probably implicating down-regulation of IKZF1/IKAROS (By similarity).
See full target information ANGPTL3

Additional targets

TGFBI,CA9,CTSB,FCER2,DPP4,CHI3L1,F3,CTLA4,Dickkopf-related protein 4,EDA,EPOR,FGF6,Fibroblast growth factor 9,FOLH1,Growth arrest-specific protein 1,IGFBP5,IL10RA,IL17C,IL18,IL18R1,IL20,IL34,IL36A,IL36B,IL36G,IL36RN,IL37,IL1F10,IL5RA,LAYN,LEPR,PRSS27,MERTK,MMP7,CDH3,Prostasin,SIGIRR,TGFBR3,TNFSF12
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websiteProtocolBooklet
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