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Abcam's Human Cytokine Antibody Array I (ab211059) for use with cell cultured media, serum, plasma, cell and tissue lysates.

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Protein Array - Human Cytokine Antibody Array I (Membrane, 37 Targets) (AB211059), expandable thumbnail

Key facts

Reactive species
Human
Target
CD244
(See target data below)

Target data

Function

Heterophilic receptor of the signaling lymphocytic activation molecule (SLAM) family; its ligand is CD48. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Acts as activating natural killer (NK) cell receptor (PubMed:10359122, PubMed:11714776, PubMed:8376943). Activating function implicates association with SH2D1A and FYN (PubMed:15713798). Downstreaming signaling involves predominantly VAV1, and, to a lesser degree, INPP5D/SHIP1 and CBL. Signal attenuation in the absence of SH2D1A is proposed to be dependent on INPP5D and to a lesser extent PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:10934222, PubMed:15713798). Stimulates NK cell cytotoxicity, production of IFN-gamma and granule exocytosis (PubMed:11714776, PubMed:8376943). Optimal expansion and activation of NK cells seems to be dependent on the engagement of CD244 with CD48 expressed on neighboring NK cells (By similarity). Acts as costimulator in NK activation by enhancing signals by other NK receptors such as NCR3 and NCR1 (PubMed:10741393). At early stages of NK cell differentiation may function as an inhibitory receptor possibly ensuring the self-tolerance of developing NK cells (PubMed:11917118). Involved in the regulation of CD8(+) T-cell proliferation; expression on activated T-cells and binding to CD48 provides costimulatory-like function for neighboring T-cells (By similarity). Inhibits inflammatory responses in dendritic cells (DCs) (By similarity).

Additional Targets

ADAM9, TNFSF13, BMP2, GDF2, C5, CTSL, CD200, ADGRE5, RARRES2, TNFRSF6B, FABP2, FAP, FGF19, LGALS3, IFNAR2, IGF2R, IL1RL2, IL24, IL33, KLK14, LGMN, OLR1, MBL2, MME, NOTCH1, CCN3, GPNMB, PDCD1, PGLYRP1, SERPINA4, FRZB, THBD, TLR2, TF, WIF1

Alternative names

CD244, 2B4, Natural killer cell receptor 2B4, NK cell activation-inducing ligand, NK cell type I receptor protein 2B4, SLAM family member 4, Signaling lymphocytic activation molecule 4, NAIL, NKR2B4, h2B4, SLAMF4

What's included?

8 Membrane
Components
1,000X HRP-Conjugated Streptavidin
1 x 50 µL
1X Blocking Buffer
2 x 25 mL
20X Wash Buffer I
1 x 20 mL
20X Wash Buffer II
1 x 20 mL
2X Cell Lysis Buffer
1 x 16 mL
8-Well Incubation Tray (with Lid)
1 x 1 Unit
Antibody Arrays
4 x 2 Unit
Biotinylated Antibody Cocktail
4 x 1 Vial
Detection Buffer C
1 x 2.5 mL
Detection Buffer D
1 x 2.5 mL
Plastic sheets
1 x 1 Unit

Recommended products

Abcam's Human Cytokine Antibody Array I (ab211059) for use with cell cultured media, serum, plasma, cell and tissue lysates.

Key facts

Reactive species
Human
Target
CD244
Applications
Multiplex Protein Detection
Sample types
Tissue Lysate, Saliva, Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture media, Sputum Supernatant, Cell culture supernatant, Milk, Serum, Other biological fluids, Whole Blood, Cell Lysate, Tissue Culture Media

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

Abcam's Human Cytokine Antibody Array I (ab211059) for use with cell cultured media, serum, plasma, cell and tissue lysates.

Targets: 2B4 (CD244), ADAM-9, APRIL, BMP-2, BMP-9, Cathepsin L, CD200, CD97, Chemerin, C5a, DcR3, RABP2, FAP, FGF-19, Galectin-3, HGFR, IFN-alpha / beta R2, IGF-2 R, IL-1 R6, IL-24, IL-33, Kallikrein 14, Legumain, LOX-1, MBL, Neprilysin, Notch-1, NOV (CCN3), Osteoactivin (GPNMB), PD-1, PGRPs, SERPINA4, sFRP-3, Thrombomodulin, TLR2, Transferrin, WIF-1.


Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1 mL of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison. "


Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

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1 product image

  • Protein Array - Human Cytokine Antibody Array I (Membrane, 37 Targets) (ab211059), expandable thumbnail

    Protein Array - Human Cytokine Antibody Array I (Membrane, 37 Targets) (ab211059)

    Typical results.

    Assay 1: Sample 1

    Assay 2: Sample 2

    Assay 3: Control

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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