AB211059

Human Cytokine Antibody Array I (Membrane, 37 Targets)

Name: Human Cytokine Antibody Array I (Membrane, 37 Targets) (ab211059) | Abcam
Brand: Abcam Limited
SKU: AB211059
Price: 925 USD
Availability: InStock

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(1 Publication)

Abcam's Human Cytokine Antibody Array I (ab211059) for use with cell cultured media, serum, plasma, cell and tissue lysates.

View Alternative Names

CD244, 2B4, Natural killer cell receptor 2B4, NK cell activation-inducing ligand, NK cell type I receptor protein 2B4, SLAM family member 4, Signaling lymphocytic activation molecule 4, NAIL, NKR2B4, h2B4, SLAMF4

1 Images

Protein Array - Human Cytokine Antibody Array I (Membrane, 37 Targets) (AB211059)

Protein Array

Supplier Data

Protein Array - Human Cytokine Antibody Array I (Membrane, 37 Targets) (AB211059)

Typical results.

Assay 1 : Sample 1

Assay 2 : Sample 2

Assay 3 : Control

Key facts

Reacts with

Human

Target

CD244

target

Applications

Multiplex Protein Detection

applications

Sample types

Tissue Lysate, Saliva, Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture media, Sputum Supernatant, Cell culture supernatant, Milk, Serum, Other biological fluids, Whole Blood, Cell Lysate, Tissue Culture Media

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Multiplex Protein Detection": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Abcam's Human Cytokine Antibody Array I (ab211059) for use with cell cultured media, serum, plasma, cell and tissue lysates.

Targets: 2B4 (CD244), ADAM-9, APRIL, BMP-2, BMP-9, Cathepsin L, CD200, CD97, Chemerin, C5a, DcR3, RABP2, FAP, FGF-19, Galectin-3, HGFR, IFN-alpha / beta R2, IGF-2 R, IL-1 R6, IL-24, IL-33, Kallikrein 14, Legumain, LOX-1, MBL, Neprilysin, Notch-1, NOV (CCN3), Osteoactivin (GPNMB), PD-1, PGRPs, SERPINA4, sFRP-3, Thrombomodulin, TLR2, Transferrin, WIF-1.

Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1 mL of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison. "

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Product protocols

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Target data

Heterophilic receptor of the signaling lymphocytic activation molecule (SLAM) family; its ligand is CD48. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Acts as activating natural killer (NK) cell receptor (PubMed : 10359122, PubMed : 11714776, PubMed : 8376943). Activating function implicates association with SH2D1A and FYN (PubMed : 15713798). Downstreaming signaling involves predominantly VAV1, and, to a lesser degree, INPP5D/SHIP1 and CBL. Signal attenuation in the absence of SH2D1A is proposed to be dependent on INPP5D and to a lesser extent PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed : 10934222, PubMed : 15713798). Stimulates NK cell cytotoxicity, production of IFN-gamma and granule exocytosis (PubMed : 11714776, PubMed : 8376943). Optimal expansion and activation of NK cells seems to be dependent on the engagement of CD244 with CD48 expressed on neighboring NK cells (By similarity). Acts as costimulator in NK activation by enhancing signals by other NK receptors such as NCR3 and NCR1 (PubMed : 10741393). At early stages of NK cell differentiation may function as an inhibitory receptor possibly ensuring the self-tolerance of developing NK cells (PubMed : 11917118). Involved in the regulation of CD8(+) T-cell proliferation; expression on activated T-cells and binding to CD48 provides costimulatory-like function for neighboring T-cells (By similarity). Inhibits inflammatory responses in dendritic cells (DCs) (By similarity).

See full target information CD244

Additional targets

ADAM9,TNFSF13,BMP2,GDF2,C5,CTSL,CD200,ADGRE5,RARRES2,TNFRSF6B,FABP2,FAP,FGF19,LGALS3,IFNAR2,IGF2R,IL1RL2,IL24,IL33,KLK14,LGMN,OLR1,MBL2,MME,NOTCH1,CCN3,GPNMB,PDCD1,PGLYRP1,SERPINA4,FRZB,THBD,TLR2,TF,WIF1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death and differentiation 31:1398-1409 PubMed38926528

2024

FRMD6 determines the cell fate towards senescence: involvement of the Hippo-YAP-CCN3 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jung-Jin Park,Su Jin Lee,Minwoo Baek,Ok-Jun Lee,Seungyoon Nam,Jaehong Kim,Jin Young Kim,Eun-Young Shin,Eung-Gook Kim

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Cytokine Antibody Array I (Membrane, 37 Targets)

Protein Array - Human Cytokine Antibody Array I (Membrane, 37 Targets) (AB211059)

Key facts

Reacts with

Target

Applications

Sample types

Reactivity data

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Additional targets

Publications (1)

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