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ab193656 can be used for the simultaneous detection of 120 Human Cytokine proteins in serum, plasma, cell culture media and other liquid samples types.

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Images

Key facts

Assay type

Semi-quantitative

Reactive species

Human

Target

AGRP

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Target data

Function

Plays a role in weight homeostasis. Involved in the control of feeding behavior through the central melanocortin system. Acts as alpha melanocyte-stimulating hormone antagonist by inhibiting cAMP production mediated by stimulation of melanocortin receptors within the hypothalamus and adrenal gland. Has very low activity with MC5R (By similarity). Is an inverse agonist for MC3R and MC4R being able to suppress their constitutive activity. It promotes MC3R and MC4R endocytosis in an arrestin-dependent manner.

Alternative names

What's included?

1 x 8 Membranes
Components
1,000X HRP-Conjugated Streptavidin
2 x 50 µL
1X Blocking Buffer
4 x 25 mL
20X Wash Buffer I
2 x 20 mL
20X Wash Buffer II
2 x 20 mL
2X Cell Lysis Buffer
2 x 16 mL
8-Well Incubation Tray (with Lid)
2 x 1 Unit
Biotinylated Antibody Cocktail (C6)
4 x 1 Vial
Biotinylated Antibody Cocktail (C7)
4 x 1 Vial
Detection Buffer C
2 x 2.5 mL
Detection Buffer D
2 x 2.5 mL
Human Cytokine Antibody Array Membranes (C6)
2 x 4 Unit
Human Cytokine Antibody Array Membranes (C7)
2 x 4 Unit
Plastic sheets
2 x 1 Unit

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ab193656 can be used for the simultaneous detection of 120 Human Cytokine proteins in serum, plasma, cell culture media and other liquid samples types.

Alternative names

Key facts

Assay type

Semi-quantitative

Reactive species

Human

Target

AGRP

Applications

Multiplex Protein Detection

Detection method

Luminescent

Sample types

Tissue Lysate, Plasma, Cell culture media, Serum, Other biological fluids, Cell Lysate

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

ab193656 can be used for the simultaneous detection of 120 Human Cytokine proteins in serum, plasma, cell culture media and other liquid samples types. It comes as 1 kit including 2 separate membranes labelled C6 and C7 (see below for images showing the array layout on each membrane)."
Targets names: Adiponectin (ACRP30), AgRP, Amphiregulin, Angiogenin, Angiopoietin-2, Axl, BDNF, bFGF, BLC (CXCL13), BMP-4, BMP-6, beta-NGF, Betacellulin (BTC), CCL28 (MEC), CK beta 8-1 (CCL23), CNTF, CTACK (CCL27), Dtk, EGF, EGFR, ENA-78 (CXCL5), Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), Eotaxin-3 (CCL26), Fas,(TNFRSF6/Apo-1), FGF-4, FGF-6, FGF-7 (KGF), FGF-9, Flt-3 Ligand, Fractalkine (CX3CL1), GCP-2 (CXCL6), GCSF, GDNF, GITR (TNFRSF18), GITR Ligand (TNFSF18), GM-CSF, GRO alpha/beta/gamma, GRO alpha (CXCL1), HCC-4 (CCL16), HGF, I-309 (TCA-3/CCL1), CCAM-1 (CD54), ICAM-3 (CD50), IFN-gamma, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IGF-1, IGF-1 R, IL-1 R4 (ST2), IL-1 R1, IL-10, IL-11, IL-12 p40, IL-12 p70, IL-13, IL-15, IL-16 IL-17A, IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-1 ra (IL-1 F3), IL-2, IL-2 R alpha, IL-3, IL-4, IL-5, IL-6, IL-6 R, IL-7, IL-8 (CXCL8), I-TAC (CXCL11), Leptin Light (TNFSF14), Lymphotactin (XCL1), MCP-1 (CCL2), MCP-2 (CCL8), MCP-3 (MARC/CCL7), MCP-4 (CCL13), M-CSF, MDC (CCL22), MIF, MIG (CXCL9), MIP-1 alpha (CCL3), MIP-1 beta (CCL4), MIP-1 delta (CCL15), MIP-3 alpha (CCL20), MIP-3 beta (CCL19), MSP alpha/beta, NAP-2 (PPBP/CXCL7), NT-3, NT-4, Oncostatin M, Osteoprotegerin (TNFRSF11B), PARC (CCL18), PDGF-BB, PLGF, RANTES (CCL5), SCF, SDF-1 alpha (CXCL12 alpha), gp130, TNF RII (TNFRSF1B), TNF RI (TNFRSF1A), TARC (CCL17), TECK (CCL25), TGF beta 3, TGF beta 1, Thrombopoietin (TPO), TIMP-1, TIMP-2, TNF alpha, TNF beta (TNFSF1B), TRAIL R3 (TNFRSF10C), TRAIL R4 (TNFRSF10D), uPAR, VEGF-A, VEGF-D.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Multiplex Protein Detection - Human Cytokine Antibody Array - Membrane (120 Targets) (ab193656), expandable thumbnail

    Multiplex Protein Detection - Human Cytokine Antibody Array - Membrane (120 Targets) (ab193656)

    Typical images obtained with Abcam Human Cytokine Antibody Array - Membrane C7 (120 Targets) ab193656. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at room temperature for 1 minute.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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