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ab169818 is for simultaneous detection of 60 Human Cytokines in 4 or 8 samples.

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Images

Multiplex Protein Detection - Human Cytokine Antibody Array - Membrane (60 targets) (AB169818), expandable thumbnail

Publications

Key facts

Assay type

Semi-quantitative

Reactive species

Human

Target

AGRP
(See target data below)

Target data

Function

Plays a role in weight homeostasis. Involved in the control of feeding behavior through the central melanocortin system. Acts as alpha melanocyte-stimulating hormone antagonist by inhibiting cAMP production mediated by stimulation of melanocortin receptors within the hypothalamus and adrenal gland. Has very low activity with MC5R (By similarity). Is an inverse agonist for MC3R and MC4R being able to suppress their constitutive activity. It promotes MC3R and MC4R endocytosis in an arrestin-dependent manner.

Targets

ANGPT2, AREG, AXL, FGF2, NGF, BTC, CCL28, CCL27, EGFR, CXCL5, FAS, FGF4, FGF9, CSF3, TNFSF18, TNFRSF18, CXCL1, CXCL2, CXCL3, CXCL1, CCL16, HGF, ICAM1, ICAM3, IGFBP3, IGFBP6, IGF1R, IL1RL1, IL1R1, IL11, IL12B, IL12A, IL17A, IL2RA, IL6R, CXCL8, CXCL11, XCL1_HUMAN, MIF, CCL3, CCL4, CCL19, MST1, NTF4, TNFRSF11B, OSM, PGF, IL6ST, TNFRSF1B, TNFRSF1A, CCL25, TIMP1, TIMP2, THPO, TNFRSF10C, TNFRSF10D, PLAUR, VEGFA, VEGFD

Alternative names

What's included?

1 x 8 Membranes
Components
1,000X HRP-Conjugated Streptavidin
1 x 50 µL
1X Blocking Buffer
2 x 25 mL
20X Wash Buffer I
1 x 20 mL
20X Wash Buffer II
1 x 20 mL
2X Cell Lysis Buffer
1 x 16 mL
8-Well Incubation Tray (with Lid)
1 x 1 Unit
Biotinylated Antibody Cocktail (C7)
4 x 1 Vial
Detection Buffer C
1 x 2.5 mL
Detection Buffer D
1 x 2.5 mL
Human Cytokine Antibody Array Membranes (C7)
2 x 4 Unit
Plastic sheets
1 x 1 Unit

Recommended products

ab169818 is for simultaneous detection of 60 Human Cytokines in 4 or 8 samples.

Key facts

Assay type

Semi-quantitative

Reactive species

Human

Target

AGRP

Applications

Multiplex Protein Detection

Sample types

Saliva, Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture media, Cell culture supernatant, Milk, Serum, Other biological fluids, Whole Blood, Cell Lysate

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

ab169818 is for simultaneous detection of 60 Human Cytokines in 4 or 8 samples. Suitable for all liquid sample types.


Targets: Adiponectin, AgRP, Angiopoietin 2, Amphiregulin, Axl, bFGF, b-NGF, BTC, CCL28, CTACK, Dtk, EGFR, ENA-78, Fas, TNFRSF6, FGF-4, FGF-9, GCSF, GITR-Ligand, GITR, GRO, GRO alpha, HCC-4, HGF, ICAM-1, ICAM-3, IGFBP-3, IGFBP-6, IGF-1 sR, IL-1 R4/ST2, IL-1 RI, IL-11, IL-12 p40, IL-12 p70, IL-17, IL-2 ra, IL-6 R, IL-8, I-TAC, Lymphotactin, MIF, MIP-1 alpha, MIP-1 beta, MIP-3 beta,MSP-alpha chain, NT-4, Osteoprotegerin, Oncostatin M, PLGF, sgp130, sTNFRII, sTNFRI, TECK, TIMP-1, TIMP-2, Thrombopoietin, TRAIL R3, TRAIL R4, uPAR, VEGF-A, VEGF-D

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Multiplex Protein Detection - Human Cytokine Antibody Array - Membrane (60 targets) (ab169818), expandable thumbnail

    Multiplex Protein Detection - Human Cytokine Antibody Array - Membrane (60 targets) (ab169818)

    Typical images obtained with Abcam Human Cytokine Antibody Array. The membranes were probed with conditioned media from two different cell lines or a mock buffer control. Membranes were exposed to film at room temperature for 1 minute.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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