Human Growth Factor Antibody Array - Membrane (41 Targets)
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(29 Publications)
Human Growth Factor Antibody Array - Membrane (41 Targets) ab134002 is a membrane based multiplex immunoassay for the simultaneous detection of 41 human growth factors in all sample types.
- More data, less sample: screen multiple growth factors with the same sample
- Global view of growth factors
- Chemiluminescent detection - no specific equipment required
- Highly sensitive (pg/mL)
View Alternative Names
AREGB, SDGF, AREG, Amphiregulin, AR, Colorectum cell-derived growth factor, CRDGF, ERBB, ERBB1, HER1, EGFR, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, HPTA, HGF, Hepatocyte growth factor, Hepatopoietin-A, Scatter factor, SF, BP2, IBP2, IGFBP2, Insulin-like growth factor-binding protein 2, IBP-2, IGF-binding protein 2, IGFBP-2, Glial cell line-derived neurotrophic factor, hGDNF, Astrocyte-derived trophic factor, ATF, GDNF, CD140b, PDGFR, PDGFR1, PDGFRB, Platelet-derived growth factor receptor beta, PDGF-R-beta, PDGFR-beta, Beta platelet-derived growth factor receptor, Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B, Platelet-derived growth factor receptor 1, PDGFR-1, CD221, Insulin-like growth factor 1 receptor, Insulin-like growth factor I receptor, IGF-I receptor, IGF1R, CD115, FMS, CSF1R, Macrophage colony-stimulating factor 1 receptor, CSF-1 receptor, Proto-oncogene c-Fms, CSF-1-R, CSF-1R, M-CSF-R, CD117, SCFR, KIT, Mast/stem cell growth factor receptor Kit, Piebald trait protein, Proto-oncogene c-Kit, Tyrosine-protein kinase Kit, p145 c-kit, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, PBT, CD309, FLK1, VEGFR2, KDR, Vascular endothelial growth factor receptor 2, VEGFR-2, Fetal liver kinase 1, Kinase insert domain receptor, Protein-tyrosine kinase receptor flk-1, FLK-1, VEGFR3, FLT4, Vascular endothelial growth factor receptor 3, VEGFR-3, Fms-like tyrosine kinase 4, Tyrosine-protein kinase receptor FLT4, FLT-4, C17orf33, GCSF, CSF3, Granulocyte colony-stimulating factor, G-CSF, Pluripoietin, FGFB, FGF2, Fibroblast growth factor 2, FGF-2, Basic fibroblast growth factor, Heparin-binding growth factor 2, bFGF, HBGF-2, PDGF2, SIS, PDGFB, Platelet-derived growth factor subunit B, PDGF subunit B, PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis, Macrophage colony-stimulating factor 1, CSF-1, M-CSF, MCSF, Lanimostim, Proteoglycan macrophage colony-stimulating factor, PG-M-CSF, CSF1, Transforming growth factor beta-2 proprotein, Cetermin, Glioblastoma-derived T-cell suppressor factor, G-TSF, TGFB2, TGFB, TGFB1, Transforming growth factor beta-1 proprotein, Transforming growth factor beta-3 proprotein, TGFB3, NGFB, NGF, Beta-nerve growth factor, Beta-NGF, IBP4, IGFBP4, Insulin-like growth factor-binding protein 4, IBP-4, IGF-binding protein 4, IGFBP-4, DTR, DTS, HEGFL, HBEGF, Proheparin-binding EGF-like growth factor, HST2, HSTF2, FGF6, Fibroblast growth factor 6, FGF-6, Heparin secretory-transforming protein 2, Heparin-binding growth factor 6, HST-2, HSTF-2, HBGF-6, Pro-epidermal growth factor, EGF, HST, HSTF1, KS3, FGF4, Fibroblast growth factor 4, FGF-4, Heparin secretory-transforming protein 1, Heparin-binding growth factor 4, Transforming protein KS3, HST-1, HSTF-1, HBGF-4, KGF, FGF7, Fibroblast growth factor 7, FGF-7, Heparin-binding growth factor 7, Keratinocyte growth factor, HBGF-7, GMCSF, CSF2, Granulocyte-macrophage colony-stimulating factor, GM-CSF, Colony-stimulating factor, Molgramostin, Sargramostim, CSF, IBP1, IGF-1, IGF1, Insulin-like growth factor 1, Insulin-like growth factor I, Mechano growth factor, Somatomedin-C, IGF-I, MGF, IBP1, IGFBP1, Insulin-like growth factor-binding protein 1, IBP-1, IGF-binding protein 1, IGFBP-1, Placental protein 12, PP12, IBP3, IGFBP3, Insulin-like growth factor-binding protein 3, IBP-3, IGF-binding protein 3, IGFBP-3, Neurotrophin-3, NT-3, HDNF, Nerve growth factor 2, Neurotrophic factor, NGF-2, NTF3, NTF5, NTF4, Neurotrophin-4, NT-4, Neurotrophin-5, Neutrophic factor 4, NT-5, PGFL, PLGF, PGF, Placenta growth factor, PlGF, MGF, SCF, KITLG, Kit ligand, Mast cell growth factor, Stem cell factor, c-Kit ligand, VEGF, VEGFA, L-VEGF, Vascular permeability factor, VPF, FIGF, VEGFD, Vascular endothelial growth factor D, VEGF-D, c-Fos-induced growth factor, IGF-2, PP1446, IGF2, Insulin-like growth factor 2, Insulin-like growth factor II, Somatomedin-A, T3M-11-derived growth factor, IGF-II, Protransforming growth factor alpha, TGFA, PDGF1, PDGFA, Platelet-derived growth factor subunit A, PDGF subunit A, PDGF-1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha polypeptide, IBP6, IGFBP6, Insulin-like growth factor-binding protein 6, IBP-6, IGF-binding protein 6, IGFBP-6, CD140a, PDGFR2, RHEPDGFRA, PDGFRA, Platelet-derived growth factor receptor alpha, PDGF-R-alpha, PDGFR-alpha, Alpha platelet-derived growth factor receptor, Alpha-type platelet-derived growth factor receptor, CD140 antigen-like family member A, CD140a antigen, Platelet-derived growth factor alpha receptor, Platelet-derived growth factor receptor 2, PDGFR-2
- Multiplex Protein Detection
Lab
Multiplex Protein Detection - Human Growth Factor Antibody Array - Membrane (41 Targets) (AB134002)
Quantification of Human Growth Factor Antibody Array.
Cells were cultured for 48 hours, washed and harvested. Cell and tissue extracts were prepared and 250 μg of total protein was assayed using ab134002. Mean pixel density was quantified using CCD camera software analysis.
- Multiplex Protein Detection
Lab
Multiplex Protein Detection - Human Growth Factor Antibody Array - Membrane (41 Targets) (AB134002)
Human serum from a pooled donor (n=50) sample was diluted to 25% and assayed using ab134002.
- Multiplex Protein Detection
Lab
Multiplex Protein Detection - Human Growth Factor Antibody Array - Membrane (41 Targets) (AB134002)
HepG2 cells (Human hepatocellular carcinoma) were seeded at 1x106 cells/mL and cultured in EMEM media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate.
Conditioned media was harvested after 48 hours, aliquoted and assayed using ab134002.
- Multiplex Protein Detection
Lab
Multiplex Protein Detection - Human Growth Factor Antibody Array - Membrane (41 Targets) (AB134002)
Quantification of Human Growth Factor Antibody Array.
Human serum from a pooled donor (n=50) sample was diluted to 25% and assayed using ab134002. Mean pixel density was quantified using CCD camera software analysis.
- Multiplex Protein Detection
Lab
Multiplex Protein Detection - Human Growth Factor Antibody Array - Membrane (41 Targets) (AB134002)
MCF7 cells (Human breast adenocarcinoma) were seeded at 1x106 cells/mL and cultured in EMEM media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Jurkat cells (Human T lymphocytes) were seeded at 1x106 cells/mL and cultured in RPMI media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate.
Cells were cultured for 48 hours, washed and harvested. Cell and tissue extracts were prepared and 250 μg of total protein was assayed using ab134002.
- Multiplex Protein Detection
Lab
Multiplex Protein Detection - Human Growth Factor Antibody Array - Membrane (41 Targets) (AB134002)
Quantification of Human Growth Factor Antibody Array.
Conditioned media was harvested after 48 hours, aliquoted and assayed using ab134002. Mean pixel density was quantified using CCD camera software analysis.
Reactivity data
Product details
ab134002 is for simultaneous detection of 41 Human Growth Factors. Suitable for all sample types.
Targets: Amphiregulin, bFGF, EGF, EGF R, FGF-4, FGF-6, FGF-7, GCSF, GDNF, GM-CSF, HB-EGF, HGF, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IGF-I, IGF-I SR, IGF-II, M-CSF, M-CSF R, beta-NGF, NT-3, NT-4, PDGF Ra, PDGF Rß, PDGF-AA, PDGF-AB, PDGF-BB, PLGF, SCF, SCF R, TGF-alpha, TGF-beta, TGF-beta2, TGF-beta3, VEGF-A, VEGF R2, VEGF R3, VEGF-D
Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
If you are interested in this cytokine array, array ab133998 may also be of interest.
A table listing all of our human membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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Properties and storage information
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Storage information
Product protocols
- Visit the General protocols
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Target data
Additional targets
Publications (29)
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PloS one 20:e0321812 PubMed40424268
2025
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Materials today. Bio 31:101592 PubMed40092225
2025
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Scientific reports 15:5483 PubMed39952961
2025
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Materials today. Bio 29:101246 PubMed39351489
2024
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Translational neurodegeneration 12:50 PubMed37946307
2023
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International journal of molecular sciences 24: PubMed37958857
2023
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Biomaterials research 27:107 PubMed37904231
2023
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Journal of inflammation research 16:3739-3761 PubMed37663761
2023
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Communications biology 5:1270 PubMed36402892
2022
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Frontiers in bioengineering and biotechnology 10:865545 PubMed35845393
2022
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Product promise
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