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ab134002 is for simultaneous detection of 41 Human Growth Factors.

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Images

Multiplex Protein Detection - Human Growth Factor Antibody Array - Membrane (41 Targets) (AB134002), expandable thumbnail
  • Multiplex Protein Detection - Human Growth Factor Antibody Array - Membrane (41 Targets) (AB134002), expandable thumbnail
  • Multiplex Protein Detection - Human Growth Factor Antibody Array - Membrane (41 Targets) (AB134002), expandable thumbnail
  • Multiplex Protein Detection - Human Growth Factor Antibody Array - Membrane (41 Targets) (AB134002), expandable thumbnail
  • Multiplex Protein Detection - Human Growth Factor Antibody Array - Membrane (41 Targets) (AB134002), expandable thumbnail

Publications

Key facts

Assay type
Semi-quantitative
Reactive species
Human
Target
AREG
(See target data below)

Target data

Function

Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.

Additional Targets

NGF, FGF2, EGF, EGFR, FGF4, FGF6, FGF7, CSF3, GDNF, CSF2, HBEGF, HGF, IGF1, IGF1R, IGF2, IGFBP1, IGFBP2, IGFBP3, IGFBP4, IGFBP6, CSF1, CSF1R, NTF3, NTF4, PDGFRA phospho Y572 + Y574, PDGFRB, PDGFA, PDGFB, PDGFB, PGF, KITLG, KIT, Protransforming growth factor alpha, TGFB1, TGFB2, TGFB3, VEGFA, VEGFD, KDR, FLT4

Alternative names

AREGB, SDGF, AREG, Amphiregulin, AR, Colorectum cell-derived growth factor, CRDGF

What's included?

1 x 4 Membranes
Components
1,000X HRP-Conjugated Streptavidin
1 x 50 µL
1X Blocking Buffer
1 x 25 mL
20X Wash Buffer I
1 x 10 mL
20X Wash Buffer II
1 x 10 mL
2X Cell Lysis Buffer
1 x 10 mL
8-Well Incubation Tray (with Lid)
1 x 1 Unit
Biotin-Conjugated Anti-Cytokines
2 x 1 Vial
Detection Buffer C
1 x 1.5 mL
Detection Buffer D
1 x 1.5 mL
Growth Factor Antibody Array Membranes
1 x 4 Unit
Plastic sheets
1 x 1 Unit

Recommended products

ab134002 is for simultaneous detection of 41 Human Growth Factors.

Key facts

Assay type
Semi-quantitative
Reactive species
Human
Target
AREG
Applications
Multiplex Protein Detection
Sample types
Saliva, Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture media, Cell culture supernatant, Milk, Serum, Other biological fluids, Whole Blood, Cell Lysate

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

ab134002 is for simultaneous detection of 41 Human Growth Factors. Suitable for all sample types.

Targets: Amphiregulin, bFGF, EGF, EGF R, FGF-4, FGF-6, FGF-7, GCSF, GDNF, GM-CSF, HB-EGF, HGF, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IGF-I, IGF-I SR, IGF-II, M-CSF, M-CSF R, beta-NGF, NT-3, NT-4, PDGF Ra, PDGF Rß, PDGF-AA, PDGF-AB, PDGF-BB, PLGF, SCF, SCF R, TGF-alpha, TGF-beta, TGF-beta2, TGF-beta3, VEGF-A, VEGF R2, VEGF R3, VEGF-D

Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.


If you are interested in this cytokine array, array ab133998 may also be of interest. **A table listing all of our human membrane antibody cytokine arrays and other arrays and the analytes they measure is available .**"


Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Multiplex Protein Detection - Human Growth Factor Antibody Array - Membrane (41 Targets) (ab134002), expandable thumbnail

    Multiplex Protein Detection - Human Growth Factor Antibody Array - Membrane (41 Targets) (ab134002)

    MCF7 cells (Human breast adenocarcinoma) were seeded at 1x106 cells/mL and cultured in EMEM media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Jurkat cells (Human T lymphocytes) were seeded at 1x106 cells/mL and cultured in RPMI media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate.

    Cells were cultured for 48 hours, washed and harvested. Cell and tissue extracts were prepared and 250 μg of total protein was assayed using ab134002.

  • Multiplex Protein Detection - Human Growth Factor Antibody Array - Membrane (41 Targets) (ab134002), expandable thumbnail

    Multiplex Protein Detection - Human Growth Factor Antibody Array - Membrane (41 Targets) (ab134002)

    Quantification of Human Growth Factor Antibody Array.

    Cells were cultured for 48 hours, washed and harvested. Cell and tissue extracts were prepared and 250 μg of total protein was assayed using ab134002. Mean pixel density was quantified using CCD camera software analysis.

  • Multiplex Protein Detection - Human Growth Factor Antibody Array - Membrane (41 Targets) (ab134002), expandable thumbnail

    Multiplex Protein Detection - Human Growth Factor Antibody Array - Membrane (41 Targets) (ab134002)

    HepG2 cells (Human hepatocellular carcinoma) were seeded at 1x106 cells/mL and cultured in EMEM media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate.

    Conditioned media was harvested after 48 hours, aliquoted and assayed using ab134002.

  • Multiplex Protein Detection - Human Growth Factor Antibody Array - Membrane (41 Targets) (ab134002), expandable thumbnail

    Multiplex Protein Detection - Human Growth Factor Antibody Array - Membrane (41 Targets) (ab134002)

    Quantification of Human Growth Factor Antibody Array.

    Conditioned media was harvested after 48 hours, aliquoted and assayed using ab134002. Mean pixel density was quantified using CCD camera software analysis.

  • Multiplex Protein Detection - Human Growth Factor Antibody Array - Membrane (41 Targets) (ab134002), expandable thumbnail

    Multiplex Protein Detection - Human Growth Factor Antibody Array - Membrane (41 Targets) (ab134002)

    Human serum from a pooled donor (n=50) sample was diluted to 25% and assayed using ab134002.

  • Multiplex Protein Detection - Human Growth Factor Antibody Array - Membrane (41 Targets) (ab134002), expandable thumbnail

    Multiplex Protein Detection - Human Growth Factor Antibody Array - Membrane (41 Targets) (ab134002)

    Quantification of Human Growth Factor Antibody Array.

    Human serum from a pooled donor (n=50) sample was diluted to 25% and assayed using ab134002. Mean pixel density was quantified using CCD camera software analysis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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