ab197452 can be used for quantitative measurement of 8 Human immunoglobulins.
Images
Key facts
- Assay type
- Quantitative
- Reactive species
- Human
- Target
- Assay Platform
- Multiplex
Target data
Function
Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268). Mediates IgG effector functions on monocytes triggering ADCC of virus-infected cells.
Alternative names
Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1
What's included?
Recommended products
ab197452 can be used for quantitative measurement of 8 Human immunoglobulins.
Key facts
- Assay type
- Quantitative
- Reactive species
- Human
- Detection method
- Fluorescent
- Assay Platform
- Multiplex
- Sample types
- Serum
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
ab197452 can be used for quantitative measurement of 8 Human immunoglobulins. Suitable for blood serum.
Target names: IgA, IgD, IgE, IgM, IgG1, IgG2, IgG3, and IgG4.Quantitative antibody arrays can be used to quantitate up to 40 cytokines with as little as 50 µL of sample. Arrays are available for 400 human and 200 mouse proteins. Each glass slide is spotted with 16 identical antibody arrays and is provided with a 16 well gasket to allow separate samples to be applied to each array. Each antibody is spotted in quadruplicate in each array. For reproducible quantitation, eight of the arrays are used with a cocktail of protein standards to produce a standard curve. The same 8 standard curve arrays can be used across multiple slides, allowing measurement of 22 experimental samples with 2 slides, 50 with 4 slides etc. For high through-put, 4 slides can be nested into a tray matching a standard microplate, allowing automated processing with a liquid handling workstation. Array processing can be completed within one working day (see workflow diagram below) and arrays can be analyzed with a wide number of laser glass slide array / gene microarray scanners. If you don’t have a suitable scanner then we recommend our membrane antibody arrays, which can be analyzed with any WB chemiluminescent reader.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Multiplex Protein Detection - Human Ig Isotyping Antibody Array (8 Targets) - Quantitative (ab197452)
Array Map for Human Ig Isotyping Antibody Array (8 Targets) - Quantitative (ab197452).
Multiplex Protein Detection - Human Ig Isotyping Antibody Array (8 Targets) - Quantitative (ab197452)
Standard Curves obtained with Abcam Human Ig Isotyping Antibody Array (8 Targets)- Quantitative (ab197452)
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com