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AB197446

Human IGF Signaling Antibody Array (10 Targets) - Quantitative

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(1 Publication)

ab197446 can be used for quantitative measurement of 10 Human cytokines.

View Alternative Names

IBP1, IGF-1, IGF1, Insulin-like growth factor 1, Insulin-like growth factor I, Mechano growth factor, Somatomedin-C, IGF-I, MGF

3 Images
Multiplex Protein Detection - Human IGF Signaling Antibody Array (10 Targets) - Quantitative (AB197446)
  • Multiplex Protein Detection

Supplier Data

Multiplex Protein Detection - Human IGF Signaling Antibody Array (10 Targets) - Quantitative (AB197446)

Array map for Abcam Human IGF Signaling Antibody Array (10 Targets) - Quantitative (ab197446).

Multiplex Protein Detection - Human IGF Signaling Antibody Array (10 Targets) - Quantitative (AB197446)
  • Multiplex Protein Detection

Supplier Data

Multiplex Protein Detection - Human IGF Signaling Antibody Array (10 Targets) - Quantitative (AB197446)

Standard curve obtained using Abcam Human IGF Signaling Antibody Array (10 Targets) - Quantitative (ab197446).

Multiplex Protein Detection - Human IGF Signaling Antibody Array (10 Targets) - Quantitative (AB197446)
  • Multiplex Protein Detection

Supplier Data

Multiplex Protein Detection - Human IGF Signaling Antibody Array (10 Targets) - Quantitative (AB197446)

Assay summary for Abcam Human IGF Signaling Antibody Array (10 Targets) - Quantitative (ab197446).

Key facts

Assay type

Quantitative

Reacts with

Human

Target

IGF1

target

Detection method

Fluorescent

Assay Platform

Multiplex

Sample types

Tissue Lysate, Plasma, Cell culture media, Serum, Other biological fluids, Cell Lysate

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Product details

ab197446 can be used for quantitative measurement of 10 Human cytokines. Suitable for serum, plasma, cell culture media, other body fluids, cell and tissue lysates.

Targets names: IGF-1, IGF-2, Insulin, IGF-1R, IGF-2R, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6.

Quantitative antibody arrays can be used to quantitate up to 40 cytokines with as little as 50 µL of sample. Arrays are available for 400 human and 200 mouse proteins. Each glass slide is spotted with 16 identical antibody arrays and is provided with a 16 well gasket to allow separate samples to be applied to each array. Each antibody is spotted in quadruplicate in each array. For reproducible quantitation, eight of the arrays are used with a cocktail of protein standards to produce a standard curve. The same 8 standard curve arrays can be used across multiple slides, allowing measurement of 22 experimental samples with 2 slides, 36 with 3 slides etc. For high through-put, 4 slides can be nested into a tray matching a standard microplate, allowing automated processing with a liquid handling workstation. Array processing can be completed within one working day (see workflow diagram below) and arrays can be analyzed with a wide number of laser glass slide array / gene microarray scanners. If you don’t have a suitable scanner then we recommend our membrane antibody arrays, which can be analyzed with any WB chemiluminescent reader. "

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation (PubMed : 21076856, PubMed : 24132240). Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb (By similarity). Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV : ITGB3 and ITGA6 : ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1 (PubMed : 19578119, PubMed : 22351760, PubMed : 23243309, PubMed : 23696648). As part of the MAPK/ERK signaling pathway, acts as a negative regulator of apoptosis in cardiomyocytes via promotion of STUB1/CHIP-mediated ubiquitination and degradation of ICER-type isoforms of CREM (By similarity).
See full target information IGF1

Additional targets

IGF1R,IGF2,IGF2R,IGFBP1,IGFBP2,IGFBP3,IGFBP4,IGFBP6,INS
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 24: PubMed34184083

2021

M2‑like tumour‑associated macrophage‑secreted IGF promotes thyroid cancer stemness and metastasis by activating the PI3K/AKT/mTOR pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Lv,Chao Liu,Fu-Kun Chen,Zhi-Ping Feng,Li Jia,Peng-Jie Liu,Zhi-Xian Yang,Fei Hou,Zhi-Yong Deng
View all publications
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