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AB197448

Human Immune Response Antibody Array (40 Targets) - Quantitative

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ab197448 can be used for quantitative measurement of 40 Human immune response-related biomarkers.

View Alternative Names

CD14, Monocyte differentiation antigen CD14, My23 antigen, Myeloid cell-specific leucine-rich glycoprotein

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Multiplex Protein Detection - Human Immune Response Antibody Array (40 Targets) - Quantitative (AB197448)
  • Multiplex Protein Detection

Supplier Data

Multiplex Protein Detection - Human Immune Response Antibody Array (40 Targets) - Quantitative (AB197448)

Array map for Abcam Human Immune Response Antibody Array (40 Targets) - Quantitative.

Multiplex Protein Detection - Human Immune Response Antibody Array (40 Targets) - Quantitative (AB197448)
  • Multiplex Protein Detection

Supplier Data

Multiplex Protein Detection - Human Immune Response Antibody Array (40 Targets) - Quantitative (AB197448)

Standard Curve obtained with Abcam Human Immune Response Antibody Array (40 Targets) - Quantitative.

Multiplex Protein Detection - Human Immune Response Antibody Array (40 Targets) - Quantitative (AB197448)
  • Multiplex Protein Detection

Supplier Data

Multiplex Protein Detection - Human Immune Response Antibody Array (40 Targets) - Quantitative (AB197448)

Assay summary for Abcam Human Immune Response Antibody Array (40 Targets) - Quantitative.

Key facts

Assay type

Quantitative

Reacts with

Human

Target

CD14

target

Detection method

Fluorescent

Assay Platform

Multiplex

Sample types

Tissue Lysate, Plasma, Cell culture media, Serum, Other biological fluids, Cell Lysate

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Product details

ab197448 can be used for quantitative measurement of 40 Human immune response-related biomarkers. Suitable for serum, plasma, cell culture media, other body fluids, cell and tissue lysates.

Targets names: CD14, CD40, CD163, CRP, E-Selectin, Fas, FasL, GCSF, ICAM-1, IL-1α, IL-1β, IL-1R4, IL-2, IL-2Ra, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-18, Lipocalin-2, MCP-1, MCP-2, MIF, MIP-1α, MIP-1β, OPN, PAI-1, PF4, PCT, RAGE, Resistin, TM, TNFα, TREM-1, Troponin 1, uPAR, VCAM-1, VEGF.

Quantitative antibody arrays can be used to quantitate up to 40 cytokines with as little as 50 µL of sample. Arrays are available for 400 human and 200 mouse proteins. Each glass slide is spotted with 16 identical antibody arrays and is provided with a 16 well gasket to allow separate samples to be applied to each array. Each antibody is spotted in quadruplicate in each array. For reproducible quantitation, eight of the arrays are used with a cocktail of protein standards to produce a standard curve. The same 8 standard curve arrays can be used across multiple slides, allowing measurement of 22 experimental samples with 2 slides, 36 with 3 slides etc. For high through-put, 4 slides can be nested into a tray matching a standard microplate, allowing automated processing with a liquid handling workstation. Array processing can be completed within one working day (see workflow diagram below) and arrays can be analyzed with a wide number of laser glass slide array / gene microarray scanners. If you don’t have a suitable scanner then we recommend our membrane antibody arrays, which can be analyzed with any WB chemiluminescent reader. The standard is affinity purified. "

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Coreceptor for bacterial lipopolysaccharide (PubMed : 1698311, PubMed : 23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed : 20133493, PubMed : 22265692, PubMed : 23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed : 8612135). Acts as a coreceptor for TLR2 : TLR6 heterodimer in response to diacylated lipopeptides and for TLR2 : TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed : 16880211). Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (PubMed : 23880187).
See full target information CD14

Additional targets

CD163,CD40,CRP,SELE,FAS,FASLG,CSF3,ICAM1,IL1A,IL1B,IL1RL1,IL10,IL12A,IL13,IL18,IL2,IL2RA,IL4,IL6,CXCL8,LCN2,CCL2,CCL8,MIF,CCL3,CCL4,SPP1,SERPINE1,PF4,CALCA,MOK,RETN,THBD,TNF,TREM1,TNNI2,PLAUR,VCAM1,VEGFA
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Product promise

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