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AB169804

Human Inflammation Antibody Array - Membrane (20 targets)

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ab169804 detects 20 Human Inflammatory Factors in 4 or 8 samples.

View Alternative Names

SCYA1, CCL1, C-C motif chemokine 1, Small-inducible cytokine A1, T lymphocyte-secreted protein I-309

5 Images
Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (20 targets) (AB169804)
  • Multiplex Protein Detection

Lab

Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (20 targets) (AB169804)

Human peripheral blood cells (1x106 cells/mL) were cultured in RPMI media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 mg/mL streptomycin sulfate.

Cells were cultured unstimulated or stimulated with 10 mg/mL PHA. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab169804. Media alone was used as a negative control.

Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (20 targets) (AB169804)
  • Multiplex Protein Detection

Lab

Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (20 targets) (AB169804)

HepG2 cells (Human hepatocellular carcinoma) were seeded at 1x106 cells/mL and cultured in EMEM media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab169804.

Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (20 targets) (AB169804)
  • Multiplex Protein Detection

Unknown

Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (20 targets) (AB169804)

Typical results obtained with Abcam Membrane Antibody Arrays. Image above presents typical images obtained with Abcam Human Cytokine Membrane Antibody Array. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at RT for 1 min.

Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (20 targets) (AB169804)
  • Multiplex Protein Detection

Lab

Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (20 targets) (AB169804)

Quantification of Human Inflammation Antibody Array.

Conditioned media was harvested after 48 hours, aliquoted and assayed using ab169804. Mean pixel density was quantified using CCD camera software analysis.

Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (20 targets) (AB169804)
  • Multiplex Protein Detection

Lab

Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (20 targets) (AB169804)

Quantification of Human Inflammation Antibody Array.

Cells were cultured unstimulated or stimulated with 10 mg/mL PHA. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab169804. Media alone was used as a negative control. Mean pixel density was quantified using CCD camera software analysis.

Key facts

Assay type

Semi-quantitative

Reacts with

Human

Target

CCL1

target

Applications

Multiplex Protein Detection

applications

Sample types

Saliva, Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture media, Cell culture supernatant, Milk, Serum, Other biological fluids, Whole Blood, Cell Lysate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Multiplex Protein Detection": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab169804 detects 20 Human Inflammatory Factors in 4 or 8 samples. Suitable for all liquid sample types.

Targets: Eotaxin-1, Eotaxin-2, G-CSF, GM-CSF, IFN-gamma, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, IL-10, IL-11, IL-12 p40, IL-12 p70, IL-13, I-309, TIMP-2

If you are interested in this cytokine array, arrays ab133996, ab133997, ab169817, ab133998 and ab134003 may also be of interest. A list of human antibody membrane arrays is available here.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Cytokine that is chemotactic for monocytes but not for neutrophils. Binds to CCR8.
See full target information CCL1

Additional targets

CCL11,CCL24,IL1A,IL1B,IL2,IL3,IL6,IL7,TIMP2,IL12B,CSF2,CSF3,CXCL8,IFNG,IL10,IL11,IL12A,IL13
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websiteProtocolBooklet
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Complementary Products

Reagents, Controls & Accessories

AB270055

Protease Inhibitor Cocktail EDTA Free (100X)

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Multiplex Kits

AB134003

Human Inflammation Antibody Array - Membrane (40 Targets)

Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (40 Targets) (AB134003)

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Multiplex Kits

AB133996

Cytokine Array - Human Cytokine Antibody Array (Membrane, 23 Targets)

Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 23 Targets) (AB133996)

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Multiplex Kits

AB133997

Cytokine Array - Human Cytokine Antibody Array (Membrane, 42 Targets)

Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 42 Targets) (AB133997)

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Multiplex Kits

AB169817

Cytokine Array - Human Cytokine Antibody Array (Membrane, 60 targets)

Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 60 targets) (AB169817)

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Product promise

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