ab169804 detects 20 Human Inflammatory Factors in 4 or 8 samples.
Images
Key facts
- Assay type
- Semi-quantitative
- Reactive species
- Human
- Target
- CCL1
(See target data below)
Target data
Function
Cytokine that is chemotactic for monocytes but not for neutrophils. Binds to CCR8.
Additional Targets
CCL11, CCL24, CSF2, CSF3, CXCL8, IFNG, IL10, Interleukin-11, IL12A, IL13, IL1A, IL1B, IL2, IL3, IL3, IL6, IL7, TIMP2, IL12B
Alternative names
SCYA1, CCL1, C-C motif chemokine 1, Small-inducible cytokine A1, T lymphocyte-secreted protein I-309
What's included?
Recommended products
ab169804 detects 20 Human Inflammatory Factors in 4 or 8 samples.
Key facts
- Assay type
- Semi-quantitative
- Reactive species
- Human
- Target
- CCL1
- Applications
- Multiplex Protein Detection
- Sample types
- Saliva, Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture media, Cell culture supernatant, Milk, Serum, Other biological fluids, Whole Blood, Cell Lysate
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
ab169804 detects 20 Human Inflammatory Factors in 4 or 8 samples. Suitable for all liquid sample types.
Targets: Eotaxin-1, Eotaxin-2, G-CSF, GM-CSF, IFN-gamma, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, IL-10, IL-11, IL-12 p40, IL-12 p70, IL-13, I-309, TIMP-2
If you are interested in this cytokine array, arrays ab133996, ab133997, ab169817, ab133998 and ab134003 may also be of interest. A list of human antibody membrane arrays is available ."
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (20 targets) (ab169804)
Human peripheral blood cells (1x106 cells/mL) were cultured in RPMI media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 mg/mL streptomycin sulfate.
Cells were cultured unstimulated or stimulated with 10 mg/mL PHA. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab169804. Media alone was used as a negative control.
Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (20 targets) (ab169804)
Quantification of Human Inflammation Antibody Array.
Cells were cultured unstimulated or stimulated with 10 mg/mL PHA. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab169804. Media alone was used as a negative control. Mean pixel density was quantified using CCD camera software analysis.
Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (20 targets) (ab169804)
HepG2 cells (Human hepatocellular carcinoma) were seeded at 1x106 cells/mL and cultured in EMEM media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab169804.
Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (20 targets) (ab169804)
Quantification of Human Inflammation Antibody Array.
Conditioned media was harvested after 48 hours, aliquoted and assayed using ab169804. Mean pixel density was quantified using CCD camera software analysis.
Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (20 targets) (ab169804)
Typical results obtained with Abcam Membrane Antibody Arrays. Image above presents typical images obtained with Abcam Human Cytokine Membrane Antibody Array. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at RT for 1 min.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com