Human Inflammation Antibody Array - Membrane (40 Targets)
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(37 Publications)
Human Inflammation Antibody Array - Membrane (40 Targets) ab134003 is a membrane based multiplex immunoassay for the simultaneous detection of 40 human inflammatory factors in all sample types.
- More data, less sample: screen multiple inflammatory markers with the same sample
- Global view of inflammatory markers
- Chemiluminescent detection - no specific equipment required
- Highly sensitive (pg/mL)
View Alternative Names
SCYA11, CCL11, Eotaxin, C-C motif chemokine 11, Eosinophil chemotactic protein, Small-inducible cytokine A11, MCP1, SCYA2, CCL2, C-C motif chemokine 2, HC11, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, Small-inducible cytokine A2, MCAF, MCP-1, CD126, Interleukin-6 receptor subunit alpha, IL-6 receptor subunit alpha, IL-6R subunit alpha, IL-6R-alpha, IL-6RA, IL-6R 1, Membrane glycoprotein 80, gp80, IL6R, CD120a, TNFAR, TNFR1, TNFRSF1A, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor 1, Tumor necrosis factor receptor type I, p55, p60, TNF-R1, TNF-RI, TNFR-I, IFNB2, IL6, Interleukin-6, IL-6, B-cell stimulatory factor 2, CTL differentiation factor, Hybridoma growth factor, Interferon beta-2, BSF-2, CDF, IFN-beta-2, C17orf33, GCSF, CSF3, Granulocyte colony-stimulating factor, G-CSF, Pluripoietin, PDGF2, SIS, PDGFB, Platelet-derived growth factor subunit B, PDGF subunit B, PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis, Macrophage colony-stimulating factor 1, CSF-1, M-CSF, MCSF, Lanimostim, Proteoglycan macrophage colony-stimulating factor, PG-M-CSF, CSF1, D17S136E, SCYA5, CCL5, C-C motif chemokine 5, EoCP, Eosinophil chemotactic cytokine, SIS-delta, Small-inducible cytokine A5, T cell-specific protein P228, T-cell-specific protein RANTES, TCP228, Interleukin-11, IL-11, Adipogenesis inhibitory factor, AGIF, IL11, IL 11, Interleukin 11, MCP2, SCYA10, SCYA8, CCL8, C-C motif chemokine 8, HC14, Monocyte chemoattractant protein 2, Monocyte chemotactic protein 2, Small-inducible cytokine A8, MCP-2, TGFB, TGFB1, Transforming growth factor beta-1 proprotein, LAG1, MIP1B, SCYA4, CCL4, C-C motif chemokine 4, G-26 T-lymphocyte-secreted protein, HC21, Lymphocyte activation gene 1 protein, MIP-1-beta(1-69), Macrophage inflammatory protein 1-beta, PAT 744, Protein H400, SIS-gamma, Small-inducible cytokine A4, T-cell activation protein 2, LAG-1, MIP-1-beta, ACT-2, CTLA8, IL17, IL17A, Interleukin-17A, IL-17, IL-17A, Cytotoxic T-lymphocyte-associated antigen 8, CTLA-8, NKSF2, IL12B, Interleukin-12 subunit beta, IL-12B, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p40, NK cell stimulatory factor chain 2, CLMF p40, INP10, SCYB10, CXCL10, C-X-C motif chemokine 10, 10 kDa interferon gamma-induced protein, Small-inducible cytokine B10, Gamma-IP10, IP-10, Metalloproteinase inhibitor 2, CSC-21K, Tissue inhibitor of metalloproteinases 2, TIMP-2, TIMP2, SCYA1, CCL1, C-C motif chemokine 1, Small-inducible cytokine A1, T lymphocyte-secreted protein I-309, Interleukin-3, IL-3, Hematopoietic growth factor, Mast cell growth factor, Multipotential colony-stimulating factor, P-cell-stimulating factor, MCGF, IL3, Interleukin-2, IL-2, T-cell growth factor, TCGF, IL2, Interleukin-4, IL-4, B-cell stimulatory factor 1, Binetrakin, Lymphocyte stimulatory factor 1, Pitrakinra, BSF-1, IL4, IL1F2, IL1B, Interleukin-1 beta, IL-1 beta, Catabolin, TNFA, TNFSF2, TNF, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, G0S19-1, MIP1A, SCYA3, CCL3, C-C motif chemokine 3, G0/G1 switch regulatory protein 19-1, Macrophage inflammatory protein 1-alpha, PAT 464.1, SIS-beta, Small-inducible cytokine A3, Tonsillar lymphocyte LD78 alpha protein, MIP-1-alpha, MPIF2, SCYA24, CCL24, C-C motif chemokine 24, CK-beta-6, Eosinophil chemotactic protein 2, Myeloid progenitor inhibitory factor 2, Small-inducible cytokine A24, Eotaxin-2, MPIF-2, GMCSF, CSF2, Granulocyte-macrophage colony-stimulating factor, GM-CSF, Colony-stimulating factor, Molgramostin, Sargramostim, CSF, CD54, Intercellular adhesion molecule 1, ICAM-1, Major group rhinovirus receptor, ICAM1, Interferon gamma, IFN-gamma, Immune interferon, IFNG, IL1F1, IL1A, Interleukin-1 alpha, IL-1 alpha, Hematopoietin-1, Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10, NC30, IL13, Interleukin-13, IL-13, Interleukin-15, IL-15, IL15, Pro-interleukin-16, IL16, Interleukin-7, IL-7, IL7, IL8, CXCL8, Interleukin-8, IL-8, C-X-C motif chemokine 8, Chemokine (C-X-C motif) ligand 8, Emoctakin, Granulocyte chemotactic protein 1, Monocyte-derived neutrophil chemotactic factor, Monocyte-derived neutrophil-activating peptide, Neutrophil-activating protein 1, Protein 3-10C, T-cell chemotactic factor, GCP-1, MDNCF, MONAP, NAP-1, CMK, MIG, SCYB9, CXCL9, C-X-C motif chemokine 9, Gamma-interferon-induced monokine, Monokine induced by interferon-gamma, Small-inducible cytokine B9, HuMIG, MIP5, NCC3, SCYA15, CCL15, C-C motif chemokine 15, Chemokine CC-2, Leukotactin-1, MIP-1 delta, Macrophage inflammatory protein 5, Mrp-2b, NCC-3, Small-inducible cytokine A15, HCC-2, LKN-1, MIP-5, TNFB, TNFSF1, LTA, Lymphotoxin-alpha, LT-alpha, TNF-beta, Tumor necrosis factor ligand superfamily member 1, NKSF1, IL12A, Interleukin-12 subunit alpha, IL-12A, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, NK cell stimulatory factor chain 1, CLMF p35, CD120b, TNFBR, TNFR2, TNFRSF1B, Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor 2, Tumor necrosis factor receptor type II, p75, p80 TNF-alpha receptor, TNF-R2, TNF-RII, TNFR-II
- Multiplex Protein Detection
Lab
Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (40 Targets) (AB134003)
Quantification of Human Inflammation Antibody Array.
Conditioned media was harvested after 48 hours, aliquoted and assayed using ab134003. Media alone was used as a negative control. Mean pixel density was quantified using CCD camera software analysis.
- Multiplex Protein Detection
Lab
Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (40 Targets) (AB134003)
Quantification of Human Inflammation Antibody Array.
Human serum from a pooled donor (n=50) sample was diluted to 25% and assayed using ab134003. Mean pixel density was quantified using CCD camera software analysis.
- Multiplex Protein Detection
Lab
Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (40 Targets) (AB134003)
Human serum from a pooled donor (n=50) sample was diluted to 25% and assayed using ab134003.
- Multiplex Protein Detection
AbReview
Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (40 Targets) (AB134003)
Left image : Conditioned media from iPSC-derived astrocytes; right image : Media only control.
Samples were incubated overnight at 4C as recommended and included the large volume wash. Images were captured using CCD camera for the exposure times indicated on the image.
Rating 5/5. Simple, sensitive and accurate method to detect multiple cytokines and growth factors from a single sample. The membranes were also consistent across the batch which allowed me to test several samples in parallel. Highly recommended.
This image is courtesy of an anonymous Abreview.
- Multiplex Protein Detection
Lab
Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (40 Targets) (AB134003)
Human peripheral blood cells (1x106 cells/mL) were cultured in RPMI media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 mg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with 10 mg/mL PHA.
Conditioned media was harvested after 48 hours, aliquoted and assayed using ab134003. Media alone was used as a negative control.
Reactivity data
Product details
ab134003 is for simultaneous detection of 40 Human Inflammatory Factors. Suitable for all sample types.
Targets: Eotaxin, Eotaxin-2, GCSF, GM-CSF, ICAM-1, IFN-gamma, I-309, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-6, IL-6sR, IL-7, IL-8, IL-10, IL-11, IL-12p40, IL-12p70, IL-13, IL-15, IL-16, IL-17, IP-10, MCP-1, MCP-2, M-CSF, MIG, MIP-1alpha, MIP-1beta, MIP-1delta, RANTES, TGF-beta1, TNF-alpha, TNF-beta, sTNF RI, sTNF-RII, PDGF-BB, TIMP-2
Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
If you are interested in this cytokine array, arrays ab133997, ab169817, ab133998, ab169804, ab169805 and ab133996 may also be of interest.
A table listing all of our human membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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Properties and storage information
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Product protocols
- Visit the General protocols
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Target data
Additional targets
Publications (37)
Recent publications for all applications. Explore the full list and refine your search
JID innovations : skin science from molecules to population health 5:100319 PubMed39867570
2025
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International journal of nanomedicine 19:12221-12255 PubMed39600409
2024
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Haematologica 110:425-438 PubMed39323416
2024
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Cancers 16: PubMed39199680
2024
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Current issues in molecular biology 46:8685-8698 PubMed39194729
2024
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Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2402757 PubMed39041892
2024
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Materials today. Bio 26:101061 PubMed38711937
2024
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Nanotechnology, science and applications 17:107-125 PubMed38645468
2024
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Science advances 9:eadj9964 PubMed38134285
2023
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Nature communications 14:7639 PubMed37993431
2023
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