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AB134003

Human Inflammation Antibody Array - Membrane (40 Targets)

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(37 Publications)

Human Inflammation Antibody Array - Membrane (40 Targets) ab134003 is a membrane based multiplex immunoassay for the simultaneous detection of 40 human inflammatory factors in all sample types.

- More data, less sample: screen multiple inflammatory markers with the same sample
- Global view of inflammatory markers
- Chemiluminescent detection - no specific equipment required
- Highly sensitive (pg/mL)

View Alternative Names

SCYA11, CCL11, Eotaxin, C-C motif chemokine 11, Eosinophil chemotactic protein, Small-inducible cytokine A11, MCP1, SCYA2, CCL2, C-C motif chemokine 2, HC11, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, Small-inducible cytokine A2, MCAF, MCP-1, CD126, Interleukin-6 receptor subunit alpha, IL-6 receptor subunit alpha, IL-6R subunit alpha, IL-6R-alpha, IL-6RA, IL-6R 1, Membrane glycoprotein 80, gp80, IL6R, CD120a, TNFAR, TNFR1, TNFRSF1A, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor 1, Tumor necrosis factor receptor type I, p55, p60, TNF-R1, TNF-RI, TNFR-I, IFNB2, IL6, Interleukin-6, IL-6, B-cell stimulatory factor 2, CTL differentiation factor, Hybridoma growth factor, Interferon beta-2, BSF-2, CDF, IFN-beta-2, C17orf33, GCSF, CSF3, Granulocyte colony-stimulating factor, G-CSF, Pluripoietin, PDGF2, SIS, PDGFB, Platelet-derived growth factor subunit B, PDGF subunit B, PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis, Macrophage colony-stimulating factor 1, CSF-1, M-CSF, MCSF, Lanimostim, Proteoglycan macrophage colony-stimulating factor, PG-M-CSF, CSF1, D17S136E, SCYA5, CCL5, C-C motif chemokine 5, EoCP, Eosinophil chemotactic cytokine, SIS-delta, Small-inducible cytokine A5, T cell-specific protein P228, T-cell-specific protein RANTES, TCP228, Interleukin-11, IL-11, Adipogenesis inhibitory factor, AGIF, IL11, IL 11, Interleukin 11, MCP2, SCYA10, SCYA8, CCL8, C-C motif chemokine 8, HC14, Monocyte chemoattractant protein 2, Monocyte chemotactic protein 2, Small-inducible cytokine A8, MCP-2, TGFB, TGFB1, Transforming growth factor beta-1 proprotein, LAG1, MIP1B, SCYA4, CCL4, C-C motif chemokine 4, G-26 T-lymphocyte-secreted protein, HC21, Lymphocyte activation gene 1 protein, MIP-1-beta(1-69), Macrophage inflammatory protein 1-beta, PAT 744, Protein H400, SIS-gamma, Small-inducible cytokine A4, T-cell activation protein 2, LAG-1, MIP-1-beta, ACT-2, CTLA8, IL17, IL17A, Interleukin-17A, IL-17, IL-17A, Cytotoxic T-lymphocyte-associated antigen 8, CTLA-8, NKSF2, IL12B, Interleukin-12 subunit beta, IL-12B, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p40, NK cell stimulatory factor chain 2, CLMF p40, INP10, SCYB10, CXCL10, C-X-C motif chemokine 10, 10 kDa interferon gamma-induced protein, Small-inducible cytokine B10, Gamma-IP10, IP-10, Metalloproteinase inhibitor 2, CSC-21K, Tissue inhibitor of metalloproteinases 2, TIMP-2, TIMP2, SCYA1, CCL1, C-C motif chemokine 1, Small-inducible cytokine A1, T lymphocyte-secreted protein I-309, Interleukin-3, IL-3, Hematopoietic growth factor, Mast cell growth factor, Multipotential colony-stimulating factor, P-cell-stimulating factor, MCGF, IL3, Interleukin-2, IL-2, T-cell growth factor, TCGF, IL2, Interleukin-4, IL-4, B-cell stimulatory factor 1, Binetrakin, Lymphocyte stimulatory factor 1, Pitrakinra, BSF-1, IL4, IL1F2, IL1B, Interleukin-1 beta, IL-1 beta, Catabolin, TNFA, TNFSF2, TNF, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, G0S19-1, MIP1A, SCYA3, CCL3, C-C motif chemokine 3, G0/G1 switch regulatory protein 19-1, Macrophage inflammatory protein 1-alpha, PAT 464.1, SIS-beta, Small-inducible cytokine A3, Tonsillar lymphocyte LD78 alpha protein, MIP-1-alpha, MPIF2, SCYA24, CCL24, C-C motif chemokine 24, CK-beta-6, Eosinophil chemotactic protein 2, Myeloid progenitor inhibitory factor 2, Small-inducible cytokine A24, Eotaxin-2, MPIF-2, GMCSF, CSF2, Granulocyte-macrophage colony-stimulating factor, GM-CSF, Colony-stimulating factor, Molgramostin, Sargramostim, CSF, CD54, Intercellular adhesion molecule 1, ICAM-1, Major group rhinovirus receptor, ICAM1, Interferon gamma, IFN-gamma, Immune interferon, IFNG, IL1F1, IL1A, Interleukin-1 alpha, IL-1 alpha, Hematopoietin-1, Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10, NC30, IL13, Interleukin-13, IL-13, Interleukin-15, IL-15, IL15, Pro-interleukin-16, IL16, Interleukin-7, IL-7, IL7, IL8, CXCL8, Interleukin-8, IL-8, C-X-C motif chemokine 8, Chemokine (C-X-C motif) ligand 8, Emoctakin, Granulocyte chemotactic protein 1, Monocyte-derived neutrophil chemotactic factor, Monocyte-derived neutrophil-activating peptide, Neutrophil-activating protein 1, Protein 3-10C, T-cell chemotactic factor, GCP-1, MDNCF, MONAP, NAP-1, CMK, MIG, SCYB9, CXCL9, C-X-C motif chemokine 9, Gamma-interferon-induced monokine, Monokine induced by interferon-gamma, Small-inducible cytokine B9, HuMIG, MIP5, NCC3, SCYA15, CCL15, C-C motif chemokine 15, Chemokine CC-2, Leukotactin-1, MIP-1 delta, Macrophage inflammatory protein 5, Mrp-2b, NCC-3, Small-inducible cytokine A15, HCC-2, LKN-1, MIP-5, TNFB, TNFSF1, LTA, Lymphotoxin-alpha, LT-alpha, TNF-beta, Tumor necrosis factor ligand superfamily member 1, NKSF1, IL12A, Interleukin-12 subunit alpha, IL-12A, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, NK cell stimulatory factor chain 1, CLMF p35, CD120b, TNFBR, TNFR2, TNFRSF1B, Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor 2, Tumor necrosis factor receptor type II, p75, p80 TNF-alpha receptor, TNF-R2, TNF-RII, TNFR-II

5 Images
Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (40 Targets) (AB134003)
  • Multiplex Protein Detection

Lab

Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (40 Targets) (AB134003)

Quantification of Human Inflammation Antibody Array.

Conditioned media was harvested after 48 hours, aliquoted and assayed using ab134003. Media alone was used as a negative control. Mean pixel density was quantified using CCD camera software analysis.

Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (40 Targets) (AB134003)
  • Multiplex Protein Detection

Lab

Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (40 Targets) (AB134003)

Quantification of Human Inflammation Antibody Array.

Human serum from a pooled donor (n=50) sample was diluted to 25% and assayed using ab134003. Mean pixel density was quantified using CCD camera software analysis.

Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (40 Targets) (AB134003)
  • Multiplex Protein Detection

Lab

Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (40 Targets) (AB134003)

Human serum from a pooled donor (n=50) sample was diluted to 25% and assayed using ab134003.

Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (40 Targets) (AB134003)
  • Multiplex Protein Detection

AbReview

Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (40 Targets) (AB134003)

Left image : Conditioned media from iPSC-derived astrocytes; right image : Media only control.

Samples were incubated overnight at 4C as recommended and included the large volume wash. Images were captured using CCD camera for the exposure times indicated on the image.

Rating 5/5. Simple, sensitive and accurate method to detect multiple cytokines and growth factors from a single sample. The membranes were also consistent across the batch which allowed me to test several samples in parallel. Highly recommended.

This image is courtesy of an anonymous Abreview.

Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (40 Targets) (AB134003)
  • Multiplex Protein Detection

Lab

Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (40 Targets) (AB134003)

Human peripheral blood cells (1x106 cells/mL) were cultured in RPMI media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 mg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with 10 mg/mL PHA.

Conditioned media was harvested after 48 hours, aliquoted and assayed using ab134003. Media alone was used as a negative control.

Key facts

Reacts with

Human

Target

CCL11

target

Applications

Multiplex Protein Detection

applications

Sample types

Saliva, Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture media, Cell culture supernatant, Milk, Serum, Other biological fluids, Whole Blood, Cell Lysate

Reactivity data

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Product details

ab134003 is for simultaneous detection of 40 Human Inflammatory Factors. Suitable for all sample types.

Targets: Eotaxin, Eotaxin-2, GCSF, GM-CSF, ICAM-1, IFN-gamma, I-309, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-6, IL-6sR, IL-7, IL-8, IL-10, IL-11, IL-12p40, IL-12p70, IL-13, IL-15, IL-16, IL-17, IP-10, MCP-1, MCP-2, M-CSF, MIG, MIP-1alpha, MIP-1beta, MIP-1delta, RANTES, TGF-beta1, TNF-alpha, TNF-beta, sTNF RI, sTNF-RII, PDGF-BB, TIMP-2

Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.

If you are interested in this cytokine array, arrays ab133997, ab169817, ab133998, ab169804, ab169805 and ab133996 may also be of interest.

A table listing all of our human membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Product protocols

Target data

Chemokine that plays a central role in both allergic and non-allergic inflammatory reactions by inducing the migration of different leukocyte types including eosinophils, basophils, macrophages and dendritic cells (PubMed : 8597956, PubMed : 33846499). Exerts its effects primarily by binding to the CCR3 receptor (PubMed : 8631813). Induces also chemotaxis of endothelial cells and promotes angiogenesis by activating the PI3K/Akt pathway (PubMed : 28279120).
See full target information CCL11

Additional targets

CCL24,CSF3,CSF2,CCL1,ICAM1,IFNG,IL1A,IL1B,IL10,IL11,IL12B,IL12A,IL13,IL15,IL16,IL17A,IL2,IL3,IL4,IL6,IL6R,IL7,CXCL8,CXCL10,CCL2,CCL8,CSF1,CXCL9,CCL3,CCL4,CCL15,PDGFB,CCL5,TGFB1,TIMP2,TNF,LTA,TNFRSF1A,TNFRSF1B

Publications (37)

Recent publications for all applications. Explore the full list and refine your search

JID innovations : skin science from molecules to population health 5:100319 PubMed39867570

2025

DNA Methyltransferase Inhibition Upregulates the Costimulatory Molecule ICAM-1 and the Immunogenic Phenotype of Melanoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Alessandra S P Cereghetti,Patrick Turko,Phil Cheng,Stephan Benke,Ala'a Al Hrout,Andreas Dzung,Reinhard Dummer,Michael O Hottiger,Richard Chahwan,Lorenza P Ferretti,Mitchell P Levesque

International journal of nanomedicine 19:12221-12255 PubMed39600409

2024

Fullerenol C(OH) Nanoparticles and Ectoine Protect Human Nasal Epithelial Cells Against the Cytokine Storm After Addition of the Full-Length Spike Protein from SARS-CoV-2.

Applications

Unspecified application

Species

Unspecified reactive species

Malwina Sosnowska,Mateusz Wierzbicki,Barbara Nasiłowska,Totka Nikolaeva Bakalova,Klara Piotrowska,Barbara Strojny-Cieślak,Ewa Sawosz,Marta Kutwin

Haematologica 110:425-438 PubMed39323416

2024

Lymphoma cell-driven IL-16 is expressed in activated B-cell-like diffuse large B-cell lymphomas and regulates the pro-tumor microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Xuwen Guan,Yi Wang,Teng Fang,Jingya Wang,Ru Li,Mu Hao,Lugui Qiu

Cancers 16: PubMed39199680

2024

Paracrine Activation of STAT3 Drives GM-CSF Expression in Breast Carcinoma Cells, Generating a Symbiotic Signaling Network with Breast Carcinoma-Associated Fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Kingsley O Osuala,Anita Chalasani,Neha Aggarwal,Kyungmin Ji,Kamiar Moin

Current issues in molecular biology 46:8685-8698 PubMed39194729

2024

Taurine and Polyphenol Complex Repaired Epidermal Keratinocyte Wounds by Regulating IL8 and TIMP2 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Sooyeon Lee,Jae Young Shin,Oh Sun Kwon,Seung-Hyun Jun,Nae-Gyu Kang

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2402757 PubMed39041892

2024

Personalized Vascularized Models of Breast Cancer Desmoplasia Reveal Biomechanical Determinants of Drug Delivery to the Tumor.

Applications

Unspecified application

Species

Unspecified reactive species

Giovanni S Offeddu,Elena Cambria,Sarah E Shelton,Kristina Haase,Zhengpeng Wan,Luca Possenti,Huu Tuan Nguyen,Mark R Gillrie,Dean Hickman,Charles G Knutson,Roger D Kamm

Materials today. Bio 26:101061 PubMed38711937

2024

3D in vitro synovial hyperplasia model on polycaprolactone-micropatterned nanofibrous microwells for screening disease-modifying anti-rheumatic drugs.

Applications

Unspecified application

Species

Unspecified reactive species

Dongwoo Kim,Jiyeon Heo,Boa Song,Gyubok Lee,Changgi Hong,Zhuomin Jiang,Sohui Lee,Kangwon Lee,Mingyo Kim,Min Hee Park

Nanotechnology, science and applications 17:107-125 PubMed38645468

2024

Farnesol and Selected Nanoparticles (Silver, Gold, Copper, and Zinc Oxide) as Effective Agents Against Biofilms Formed by Pathogenic Microorganisms.

Applications

Unspecified application

Species

Unspecified reactive species

Agata Lange,Arkadiusz Matuszewski,Marta Kutwin,Agnieszka Ostrowska,Sławomir Jaworski

Science advances 9:eadj9964 PubMed38134285

2023

Cellular immunity analysis by a modular acoustofluidic platform: CIAMAP.

Applications

Unspecified application

Species

Unspecified reactive species

Ruoyu Zhong,Matthew Sullivan,Neil Upreti,Roy Chen,Agustin De Ganzó,Kaichun Yang,Shujie Yang,Ke Jin,Ye He,Ke Li,Jianping Xia,Zhiteng Ma,Luke P Lee,Tania Konry,Tony Jun Huang

Nature communications 14:7639 PubMed37993431

2023

Acoustofluidic Interfaces for the Mechanobiological Secretome of MSCs.

Applications

Unspecified application

Species

Unspecified reactive species

Ye He,Shujie Yang,Pengzhan Liu,Ke Li,Ke Jin,Ryan Becker,Jinxin Zhang,Chuanchuan Lin,Jianping Xia,Zhehan Ma,Zhiteng Ma,Ruoyu Zhong,Luke P Lee,Tony Jun Huang
View all publications
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