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ab134003 is for simultaneous detection of 40 Human Inflammatory Factors.

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Images

Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (40 Targets) (AB134003), expandable thumbnail
  • Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (40 Targets) (AB134003), expandable thumbnail
  • Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (40 Targets) (AB134003), expandable thumbnail
  • Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (40 Targets) (AB134003), expandable thumbnail
  • Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (40 Targets) (AB134003), expandable thumbnail

Publications

Key facts

Assay type
Semi-quantitative
Reactive species
Human
Target
CCL11
(See target data below)

Target data

Function

In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions (PubMed:8597956). Binds to CCR3 (PubMed:8631813).

Additional Targets

CCL24, CSF3, CSF2, CCL1, ICAM1, IFNG, IL1A, IL1B, IL10, Interleukin-11, IL12B, IL12A, IL13, IL15, IL16, IL17A, IL2, IL3, IL4, IL6, IL6R, IL7, CXCL8, CXCL10, CCL2, CCL8, CSF1, CXCL9, CCL3, CCL4, CCL15, PDGFB, CCL5, TGFB1, TIMP2, TNF, LTA, TNFRSF1A, TNFRSF1B

Alternative names

SCYA11, CCL11, Eotaxin, C-C motif chemokine 11, Eosinophil chemotactic protein, Small-inducible cytokine A11

What's included?

1 x 8 Membranes
Components
1,000X HRP-Conjugated Streptavidin
1 x 50 µL
1X Blocking Buffer
2 x 25 mL
20X Wash Buffer I
1 x 20 mL
20X Wash Buffer II
1 x 20 mL
2X Cell Lysis Buffer
1 x 16 mL
8-Well Incubation Tray (with Lid)
1 x 1 Unit
Biotin-Conjugated Anti-Cytokines
4 x 1 Vial
Detection Buffer C
1 x 2.5 mL
Detection Buffer D
1 x 2.5 mL
Inflammation Antibody Array Membranes
2 x 4 Unit
ab134003
Plastic sheets
1 x 1 Unit

Recommended products

ab134003 is for simultaneous detection of 40 Human Inflammatory Factors.

Key facts

Assay type
Semi-quantitative
Reactive species
Human
Target
CCL11
Applications
Multiplex Protein Detection
Sample types
Saliva, Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture media, Cell culture supernatant, Milk, Serum, Other biological fluids, Whole Blood, Cell Lysate

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

ab134003 is for simultaneous detection of 40 Human Inflammatory Factors. Suitable for all sample types.

Targets: Eotaxin, Eotaxin-2, GCSF, GM-CSF, ICAM-1, IFN-gamma, I-309, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-6, IL-6sR, IL-7, IL-8, IL-10, IL-11, IL-12p40, IL-12p70, IL-13, IL-15, IL-16, IL-17, IP-10, MCP-1, MCP-2, M-CSF, MIG, MIP-1alpha, MIP-1beta, MIP-1delta, RANTES, TGF-beta1, TNF-alpha, TNF-beta, sTNF RI, sTNF-RII, PDGF-BB, TIMP-2

Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.


If you are interested in this cytokine array, arrays ab133997, ab169817, ab133998, ab169804, ab169805 and ab133996 may also be of interest. **A table listing all of our human membrane antibody cytokine arrays and other arrays and the analytes they measure is available .** "


Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (40 Targets) (ab134003), expandable thumbnail

    Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (40 Targets) (ab134003)

    Human peripheral blood cells (1x106 cells/mL) were cultured in RPMI media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 mg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with 10 mg/mL PHA.

    Conditioned media was harvested after 48 hours, aliquoted and assayed using ab134003. Media alone was used as a negative control.

  • Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (40 Targets) (ab134003), expandable thumbnail

    Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (40 Targets) (ab134003)

    Quantification of Human Inflammation Antibody Array.

    Conditioned media was harvested after 48 hours, aliquoted and assayed using ab134003. Media alone was used as a negative control. Mean pixel density was quantified using CCD camera software analysis.

  • Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (40 Targets) (ab134003), expandable thumbnail

    Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (40 Targets) (ab134003)

    Human serum from a pooled donor (n=50) sample was diluted to 25% and assayed using ab134003.

  • Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (40 Targets) (ab134003), expandable thumbnail

    Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (40 Targets) (ab134003)

    Quantification of Human Inflammation Antibody Array.

    Human serum from a pooled donor (n=50) sample was diluted to 25% and assayed using ab134003. Mean pixel density was quantified using CCD camera software analysis.

  • Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (40 Targets) (ab134003), expandable thumbnail
    This image is courtesy of an anonymous customer review.

    Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (40 Targets) (ab134003)

    Left image: Conditioned media from iPSC-derived astrocytes; right image: Media only control.

    Samples were incubated overnight at 4C as recommended and included the large volume wash. Images were captured using CCD camera for the exposure times indicated on the image.

    Rating 5/5. Simple, sensitive and accurate method to detect multiple cytokines and growth factors from a single sample. The membranes were also consistent across the batch which allowed me to test several samples in parallel. Highly recommended.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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