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AB197453

Human MMP Antibody Array (10 Targets) - Quantitative

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(2 Publications)

ab197453 can be used for quantitative measurement of 10 Human MMPs.

View Alternative Names

CLG, MMP1, Interstitial collagenase, Fibroblast collagenase, Matrix metalloproteinase-1, MMP-1

2 Images
Multiplex Protein Detection - Human MMP Antibody Array (10 Targets) - Quantitative (AB197453)
  • Multiplex Protein Detection

Supplier Data

Multiplex Protein Detection - Human MMP Antibody Array (10 Targets) - Quantitative (AB197453)

Standard Curves obtained with Abcam Human MMP Antibody Array (10 Targets) - Quantitative (ab197453).

Multiplex Protein Detection - Human MMP Antibody Array (10 Targets) - Quantitative (AB197453)
  • Multiplex Protein Detection

Supplier Data

Multiplex Protein Detection - Human MMP Antibody Array (10 Targets) - Quantitative (AB197453)

Array Map for Human MMP Antibody Array (10 Targets) - Quantitative (ab197453).

Key facts

Assay type

Quantitative

Reacts with

Human

Target

MMP1

target

Detection method

Fluorescent

Assay Platform

Multiplex

Sample types

Tissue Lysate, Plasma, Cell culture media, Serum, Other biological fluids, Cell Lysate

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Product details

ab197453 can be used for quantitative measurement of 10 Human MMPs. Suitable for serum, plasma, cell culture media, other body fluids, cell and tissue lysates.

Target names: MMP-1, MMP-2, MMP-3, MMP-8, MMP-9, MMP-10, MMP-13, TIMP-1, TIMP-2, TIMP-4.

Quantitative antibody arrays can be used to quantitate up to 40 cytokines with as little as 50 µL of sample. Arrays are available for 400 human and 200 mouse proteins. Each glass slide is spotted with 16 identical antibody arrays and is provided with a 16 well gasket to allow separate samples to be applied to each array. Each antibody is spotted in quadruplicate in each array. For reproducible quantitation, eight of the arrays are used with a cocktail of protein standards to produce a standard curve. The same 8 standard curve arrays can be used across multiple slides, allowing measurement of 22 experimental samples with 2 slides, 36 with 3 slides etc. For high through-put, 4 slides can be nested into a tray matching a standard microplate, allowing automated processing with a liquid handling workstation. Array processing can be completed within one working day (see workflow diagram below) and arrays can be analyzed with a wide number of laser glass slide array / gene microarray scanners. If you don’t have a suitable scanner then we recommend our membrane antibody arrays, which can be analyzed with any WB chemiluminescent reader. The standard is affinity purified. "

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X (PubMed : 1645757, PubMed : 2153297, PubMed : 2557822). In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity (PubMed : 16807369).
See full target information MMP1

Additional targets

MMP10,MMP13,MMP2,MMP3,MMP8,TIMP1,TIMP2,Metalloproteinase inhibitor 4
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 21: PubMed33143089

2020

Hypoxia Regulates DPP4 Expression, Proteolytic Inactivation, and Shedding from Ovarian Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Laura R Moffitt,Maree Bilandzic,Amy L Wilson,Yiqian Chen,Mark D Gorrell,Martin K Oehler,Magdalena Plebanski,Andrew N Stephens

Molecular cancer research : MCR 15:1398-1409 PubMed28637905

2017

p120-Catenin Downregulation and Mutations Cooperate to Induce Invasion through MMP1 in HNSCC.

Applications

Unspecified application

Species

Unspecified reactive species

Michal Kidacki,Heather L Lehman,Michelle V Green,Joshua I Warrick,Douglas B Stairs
View all publications
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