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AB134004

Human MMP Antibody Array - Membrane (10 Targets)

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(21 Publications)

Human MMP Antibody Array - Membrane (10 Targets) ab134004 is a membrane based multiplex immunoassay for the simultaneous detection of 10 human Matrix Metalloproteinasea (MMPs) in all sample types.

- More data, less sample: screen multiple MMPs with the same sample
- Global view of MMPs expression
- Chemiluminescent detection - no specific equipment required
- Highly sensitive (pg/mL)

View Alternative Names

CLG, MMP1, Interstitial collagenase, Fibroblast collagenase, Matrix metalloproteinase-1, MMP-1, CLG4A, MMP2, 72 kDa type IV collagenase, 72 kDa gelatinase, Gelatinase A, Matrix metalloproteinase-2, TBE-1, MMP-2, Metalloproteinase inhibitor 2, CSC-21K, Tissue inhibitor of metalloproteinases 2, TIMP-2, TIMP2, Metalloproteinase inhibitor 4, Tissue inhibitor of metalloproteinases 4, TIMP-4, TIMP4, STMY1, MMP3, Stromelysin-1, SL-1, Matrix metalloproteinase-3, Transin-1, MMP-3, Collagenase 3, Matrix metalloproteinase-13, MMP-13, MMP13, CLGI, TIMP, TIMP1, Metalloproteinase inhibitor 1, Erythroid-potentiating activity, Fibroblast collagenase inhibitor, Tissue inhibitor of metalloproteinases 1, EPA, Collagenase inhibitor, TIMP-1, CLG1, MMP8, Neutrophil collagenase, Matrix metalloproteinase-8, PMNL collagenase, MMP-8, PMNL-CL, STMY2, MMP10, Stromelysin-2, SL-2, Matrix metalloproteinase-10, Transin-2, MMP-10

5 Images
Multiplex Protein Detection - Human MMP Antibody Array - Membrane (10 Targets) (AB134004)
  • Multiplex Protein Detection

Lab

Multiplex Protein Detection - Human MMP Antibody Array - Membrane (10 Targets) (AB134004)

Quantification of Human MMP Antibody Array.

Human serum and plasma from a pooled donor (n=50) sample was diluted to 25% and assayed using ab134004. Mean pixel density was quantified using CCD camera software analysis.

Dot Blot - Human MMP Antibody Array - Membrane (10 Targets) (AB134004)
  • Dot

Supplier Data

Dot Blot - Human MMP Antibody Array - Membrane (10 Targets) (AB134004)

Membranes were probed with media and cell lysate from control and treated MCF-7 cells. These membranes were exposed to film for 1 minute.

This image is courtesy of an anonymous customer.

Multiplex Protein Detection - Human MMP Antibody Array - Membrane (10 Targets) (AB134004)
  • Multiplex Protein Detection

Lab

Multiplex Protein Detection - Human MMP Antibody Array - Membrane (10 Targets) (AB134004)

143B cells (Human osteosarcoma) were seeded at 1x106 cells/mL and cultured in EMEM media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate.

Cells were cultured for 48 hours, washed and harvested. Cell and tissue extracts were prepared and 250 μg of total protein was assayed using ab134004.

Multiplex Protein Detection - Human MMP Antibody Array - Membrane (10 Targets) (AB134004)
  • Multiplex Protein Detection

Lab

Multiplex Protein Detection - Human MMP Antibody Array - Membrane (10 Targets) (AB134004)

Quantification of Human MMP Antibody Array.

Cells were cultured for 48 hours, washed and harvested. Cell and tissue extracts were prepared and 250 μg of total protein was assayed using ab134004. Mean pixel density was quantified using CCD camera software analysis.

Multiplex Protein Detection - Human MMP Antibody Array - Membrane (10 Targets) (AB134004)
  • Multiplex Protein Detection

Lab

Multiplex Protein Detection - Human MMP Antibody Array - Membrane (10 Targets) (AB134004)

Human serum and plasma from a pooled donor (n=50) sample was diluted to 25% and assayed using ab134004.

Key facts

Reacts with

Human

Target

MMP1

target

Applications

Multiplex Protein Detection

applications

Sample types

Saliva, Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture media, Cell culture supernatant, Milk, Serum, Other biological fluids, Whole Blood, Cell Lysate

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Complementary Products

Reagents, Controls & Accessories

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AB133997

Cytokine Array - Human Cytokine Antibody Array (Membrane, 42 Targets)

Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 42 Targets) (AB133997)

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AB133998

Cytokine Array - Human Cytokine Antibody Array (Membrane, 80 Targets)

Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 80 Targets) (AB133998)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Multiplex Kits

AB134001

Human Apoptosis Antibody Array - Membrane (43 Targets)

Multiplex Protein Detection - Human Apoptosis Antibody Array - Membrane (43 Targets) (AB134001)

  • 1
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Multiplex Protein Detection": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab134004 is for simultaneous detection of 7 Human MMPs and 3 TIMPs. Suitable for all sample types.

Targets: MMP-1, MMP-2, MMP-3, MMP-8, MMP-9, MMP-10, MMP-13, TIMP-1, TIMP-2, TIMP-4

Antibody arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The antibody array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.

If you are interested in this antibody array, array ab169816 may also be of interest.

A table of human antibody membrane arrays and the analytes they measure is available here.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X (PubMed : 1645757, PubMed : 2153297, PubMed : 2557822). In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity (PubMed : 16807369).
See full target information MMP1

Additional targets

MMP2,MMP3,MMP8,MMP10,MMP13,TIMP1,TIMP2,Metalloproteinase inhibitor 4
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Publications (21)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:19970 PubMed40481143

2025

Dysregulation of cell migration by matrix metalloproteinases in geleophysic dysplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Alejo A Morales,Vladimir Camarena,LéShon Peart,Sarah Smithson,Lindsay Shaw,Lucy Webber,Jose M Negron,Juan E Sola,Ann-Christina Brady,Katherina Walz,Gaofeng Wang,Mustafa Tekin

Journal of cellular and molecular medicine 29:e70498 PubMed40159638

2025

Kaempferol Targets Global Epigenetic Modifiers to Impedes Growth and Migratory Ability of HeLa Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Nazia Afroze,Shafiul Haque,Arif Hussain

Scientific reports 14:26421 PubMed39488561

2024

Hepatocyte growth factor upregulates MMP1 and MMP10 expression and resolves corneal fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Mingshun Lee,Elsayed Elbasiony,Wonkyung J Cho,Vinay K Pulimamidi,Olufemi S Folorunso,Sharad K Mittal,Reza Dana,Sunil K Chauhan

Cancers 15: PubMed38067256

2023

Influence of C Nanofilm on the Expression of Selected Markers of Mesenchymal-Epithelial Transition in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Malwina Sosnowska,Marta Kutwin,Katarzyna Zawadzka,Michał Pruchniewski,Barbara Strojny,Zuzanna Bujalska,Mateusz Wierzbicki,Sławomir Jaworski,Ewa Sawosz

Biomolecules 13: PubMed37509102

2023

The Effects of Natural Epigenetic Therapies in 3D Ovarian Cancer and Patient-Derived Tumor Explants: New Avenues in Regulating the Cancer Secretome.

Applications

Unspecified application

Species

Unspecified reactive species

Rebeca Kelly,Diego Aviles,Catriona Krisulevicz,Krystal Hunter,Lauren Krill,David Warshal,Olga Ostrovsky

Cancers 15: PubMed37370838

2023

The Mutation G17V Does Not Lead to Increased Migration of Human Malignant T Cells but Is Associated with Matrix Remodelling.

Applications

Unspecified application

Species

Unspecified reactive species

Katrin Merk-Ahmad,Julia Bein,Sonja Scharf,Hendrik Schäfer,Tobias Bexte,Evelyn Ullrich,Andreas G Loth,Nadine Flinner,Tina Senff,Olga Schneider,Martin-Leo Hansmann,Matthieu Piel,Björn Häupl,Thomas Oellerich,Emmanuel Donnadieu,Sylvia Hartmann

Journal of orthopaedic translation 38:141-155 PubMed36381245

2022

Therapeutic effect of induced pluripotent stem cell -derived extracellular vesicles in an and osteoarthritis model.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Huan Hsueh,Waradee Buddhakosai,Phung Ngan Le,Yung-Yi Tu,Hsien-Chang Huang,Huai-En Lu,Wen-Liang Chen,Yuan-Kun Tu

Oncology reports 47: PubMed35485275

2022

Cancer cell‑induced tissue inhibitor of metalloproteinase‑1 secretion by cancer‑associated fibroblasts promotes cancer cell migration.

Applications

Unspecified application

Species

Unspecified reactive species

Nozomu Nakai,Masayasu Hara,Hiroki Takahashi,Kazuyoshi Shiga,Takahisa Hirokawa,Yuzo Maeda,Takeshi Yanagita,Nanako Ando,Korehito Takasu,Takuya Suzuki,Anri Maeda,Ryo Ogawa,Yoichi Matsuo,Shuji Takiguchi

Molecular and cellular endocrinology 534:111368 PubMed34153378

2021

Differential control of uterine artery endothelial monolayer integrity by TNF and VEGF is achieved through multiple mechanisms operating inside and outside the cell - Relevance to preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda C Ampey,Rachel L Dahn,Mary A Grummer,Ian M Bird

Experimental and therapeutic medicine 22:808 PubMed34093764

2021

Blocking TNFα attenuates progressive cartilage matrix degradation in inflammatory arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Jinsung Park,Hyosun Park,Young Lim Lee,Subin Weon,Yong-Gil Kim,Jae-Hyuk Yang,Bora Nam,Sungsin Jo,Tae-Hwan Kim
View all publications
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