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ab134004 is for simultaneous detection of 7 Human MMPs and 3 TIMPs.

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Images

Multiplex Protein Detection - Human MMP Antibody Array - Membrane (10 Targets) (AB134004), expandable thumbnail
  • Multiplex Protein Detection - Human MMP Antibody Array - Membrane (10 Targets) (AB134004), expandable thumbnail
  • Multiplex Protein Detection - Human MMP Antibody Array - Membrane (10 Targets) (AB134004), expandable thumbnail
  • Multiplex Protein Detection - Human MMP Antibody Array - Membrane (10 Targets) (AB134004), expandable thumbnail
  • Dot Blot - Human MMP Antibody Array - Membrane (10 Targets) (AB134004), expandable thumbnail

Publications

Key facts

Assay type
Semi-quantitative
Reactive species
Human
Target
MMP1
(See target data below)

Target data

Function

Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X (PubMed:1645757, PubMed:2153297, PubMed:2557822). In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity (PubMed:16807369).

Additional Targets

MMP2, MMP3, MMP8, MMP10, MMP13, TIMP1, TIMP2, Metalloproteinase inhibitor 4

Alternative names

CLG, MMP1, Interstitial collagenase, Fibroblast collagenase, Matrix metalloproteinase-1, MMP-1

What's included?

1 x 4 Membranes
Components
1,000X HRP-Conjugated Streptavidin
1 x 50 µL
1X Blocking Buffer
1 x 25 mL
20X Wash Buffer I
1 x 10 mL
20X Wash Buffer II
1 x 10 mL
2X Cell Lysis Buffer
1 x 10 mL
8-Well Incubation Tray (with Lid)
1 x 1 Unit
Biotin-Conjugated Anti-Cytokines
2 x 1 Vial
Cytokine Antibody Array Membranes
1 x 4 Unit
Detection Buffer C
1 x 1.5 mL
Detection Buffer D
1 x 1.5 mL
Plastic sheets
1 x 1 Unit

Recommended products

ab134004 is for simultaneous detection of 7 Human MMPs and 3 TIMPs.

Key facts

Assay type
Semi-quantitative
Reactive species
Human
Target
MMP1
Applications
Multiplex Protein Detection
Sample types
Saliva, Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture media, Cell culture supernatant, Milk, Serum, Other biological fluids, Whole Blood, Cell Lysate

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

ab134004 is for simultaneous detection of 7 Human MMPs and 3 TIMPs. Suitable for all sample types.

Targets: MMP-1, MMP-2, MMP-3, MMP-8, MMP-9, MMP-10, MMP-13, TIMP-1, TIMP-2, TIMP-4

Antibody arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The antibody array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.


If you are interested in this antibody array, array ab169816 may also be of interest. **A table of human antibody membrane arrays and the analytes they measure is available .**"


Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Multiplex Protein Detection - Human MMP Antibody Array - Membrane (10 Targets) (ab134004), expandable thumbnail

    Multiplex Protein Detection - Human MMP Antibody Array - Membrane (10 Targets) (ab134004)

    Human serum and plasma from a pooled donor (n=50) sample was diluted to 25% and assayed using ab134004.

  • Multiplex Protein Detection - Human MMP Antibody Array - Membrane (10 Targets) (ab134004), expandable thumbnail

    Multiplex Protein Detection - Human MMP Antibody Array - Membrane (10 Targets) (ab134004)

    Quantification of Human MMP Antibody Array.

    Human serum and plasma from a pooled donor (n=50) sample was diluted to 25% and assayed using ab134004. Mean pixel density was quantified using CCD camera software analysis.

  • Multiplex Protein Detection - Human MMP Antibody Array - Membrane (10 Targets) (ab134004), expandable thumbnail

    Multiplex Protein Detection - Human MMP Antibody Array - Membrane (10 Targets) (ab134004)

    143B cells (Human osteosarcoma) were seeded at 1x106 cells/mL and cultured in EMEM media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate.

    Cells were cultured for 48 hours, washed and harvested. Cell and tissue extracts were prepared and 250 μg of total protein was assayed using ab134004.

  • Multiplex Protein Detection - Human MMP Antibody Array - Membrane (10 Targets) (ab134004), expandable thumbnail

    Multiplex Protein Detection - Human MMP Antibody Array - Membrane (10 Targets) (ab134004)

    Quantification of Human MMP Antibody Array.

    Cells were cultured for 48 hours, washed and harvested. Cell and tissue extracts were prepared and 250 μg of total protein was assayed using ab134004. Mean pixel density was quantified using CCD camera software analysis.

  • Dot Blot - Human MMP Antibody Array - Membrane (10 Targets) (ab134004), expandable thumbnail
    This image is courtesy of an anonymous customer.

    Dot Blot - Human MMP Antibody Array - Membrane (10 Targets) (ab134004)

    Membranes were probed with media and cell lysate from control and treated MCF-7 cells. These membranes were exposed to film for 1 minute.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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