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Abcam's Human Neuro Antibody Array II (ab211063) for use with cell culture media, serum, plasma, cell and tissue lysates and other liquid samples.

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Multiplex Protein Detection - Human Neuro Antibody Array II (Membrane, 30 Targets) (AB211063), expandable thumbnail

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Key facts

Reactive species
Human
Target
BDNF
(See target data below)

Target data

Function

The protein expressed by the BDNF gene is a critical signaling molecule that activates pathways downstream of NTRK2, as well as the heterodimeric receptor formed by NGFR and SORCS2. During development, it aids the survival and differentiation of specific neuronal populations in both the peripheral and central nervous systems. It is involved in axonal growth, pathfinding, and modulation of dendritic growth and morphology. In adult synapses, BDNF is a key regulator of synaptic transmission and plasticity in various CNS regions, contributing to processes like long-term potentiation, long-term depression, some short-term synaptic plasticity forms, and the homeostatic regulation of intrinsic neuronal excitability. Signaling through NGFR and SORCS2 is linked to synaptic plasticity and long-term depression, while its binding to these receptors also promotes neuronal apoptosis. This supplementary information is collated from multiple sources and compiled automatically.

Additional Targets

NGF, CNTF, CRP, CCL11, CCL24, CCL26, FAS, GDNF, CSF2, IFNG, IL1A, IL1B, IL10, IL18, IL4, IL6, CXCL8, LIF, CCL2, CCL4, MMP2, MMP3, S100B, CCL17, TGFB1, TIMP1, TNF, VEGFA

Alternative names

Neurotrophic factor BDNF precursor form, proBDNF, Abrineurin, Brain-derived neurotrophic factor, BDNF

What's included?

8 Membrane
Components
1,000X HRP-Conjugated Streptavidin
1 x 50 µL
1X Blocking Buffer
2 x 25 mL
20X Wash Buffer I
1 x 20 mL
20X Wash Buffer II
1 x 20 mL
2X Cell Lysis Buffer
1 x 16 mL
8-Well Incubation Tray (with Lid)
1 x 1 Unit
Antibody Arrays
4 x 2 Unit
Biotinylated Antibody Cocktail
4 x 1 Vial
Detection Buffer C
1 x 2.5 mL
Detection Buffer D
1 x 2.5 mL
Plastic sheets
1 x 1 Unit

Recommended products

Abcam's Human Neuro Antibody Array II (ab211063) for use with cell culture media, serum, plasma, cell and tissue lysates and other liquid samples.

Key facts

Reactive species
Human
Target
BDNF
Applications
Multiplex Protein Detection
Sample types
Tissue Lysate, Plasma, Cell culture media, Serum, Other biological fluids, Cell Lysate

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

Abcam's Human Neuro Antibody Array II (ab211063) for use with cell culture media, serum, plasma, cell and tissue lysates and other liquid samples.

Targets: Adiponectin, BDNF, beta-NGF, CNTF, CRP, Eotaxin-1, Eotaxin-2, Eotaxin-3, Fas, IL-4, GDNF, GM-CSF, IFN-gamma, IL-10, IL-18, IL-1 alpha, IL-1 beta, IL-6, IL-8, LIF, MCP-1, MIP-1 beta, MMP-2, MMP-3, S100B, TARC, TGF beta, TIMP-1, TNF alpha, VEGF-A.


Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1 mL of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison. "


Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Multiplex Protein Detection - Human Neuro Antibody Array II (Membrane, 30 Targets) (ab211063), expandable thumbnail

    Multiplex Protein Detection - Human Neuro Antibody Array II (Membrane, 30 Targets) (ab211063)

    Typical results.

    Membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at room temperature for 1 minute.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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