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AB197417

Human Obesity Antibody Array (40 Targets) - Quantitative

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ab197417 can be used for quantitative measurement of 40 Human cytokines.

View Alternative Names

DF, PFD, CFD, Complement factor D, Adipsin, C3 convertase activator, Properdin factor D, Prolactin, PRL, PRO2222, RBP4, Retinol-binding protein 4, Plasma retinol-binding protein, PRBP, RBP, Neurotrophic factor BDNF precursor form, proBDNF, Abrineurin, Brain-derived neurotrophic factor, BDNF, PAI1, PLANH1, SERPINE1, Plasminogen activator inhibitor 1, PAI, PAI-1, Endothelial plasminogen activator inhibitor, Serpin E1, BP2, IBP2, IGFBP2, Insulin-like growth factor-binding protein 2, IBP-2, IGF-binding protein 2, IGFBP-2, CD120a, TNFAR, TNFR1, TNFRSF1A, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor 1, Tumor necrosis factor receptor type I, p55, p60, TNF-R1, TNF-RI, TNFR-I, Gastricsin, Pepsinogen C, PGC, IFNB2, IL6, Interleukin-6, IL-6, B-cell stimulatory factor 2, CTL differentiation factor, Hybridoma growth factor, Interferon beta-2, BSF-2, CDF, IFN-beta-2, PDGF2, SIS, PDGFB, Platelet-derived growth factor subunit B, PDGF subunit B, PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis, HNL, NGAL, LCN2, Neutrophil gelatinase-associated lipocalin, 25 kDa alpha-2-microglobulin-related subunit of MMP-9, Lipocalin-2, Oncogene 24p3, Siderocalin, p25, D17S136E, SCYA5, CCL5, C-C motif chemokine 5, EoCP, Eosinophil chemotactic cytokine, SIS-delta, Small-inducible cytokine A5, T cell-specific protein P228, T-cell-specific protein RANTES, TCP228, CALC1, CALCA, Calcitonin, TGFB, TGFB1, Transforming growth factor beta-1 proprotein, PTX1, CRP, C-reactive protein, NKSF2, IL12B, Interleukin-12 subunit beta, IL-12B, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p40, NK cell stimulatory factor chain 2, CLMF p40, Insulin, INS, IL1F2, IL1B, Interleukin-1 beta, IL-1 beta, Catabolin, TNFA, TNFSF2, TNF, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, AGRT, ART, AGRP, Agouti-related protein, Interferon gamma, IFN-gamma, Immune interferon, IFNG, IBP1, IGF-1, IGF1, Insulin-like growth factor 1, Insulin-like growth factor I, Mechano growth factor, Somatomedin-C, IGF-I, MGF, IBP1, IGFBP1, Insulin-like growth factor-binding protein 1, IBP-1, IGF-binding protein 1, IGFBP-1, Placental protein 12, PP12, IL1F3, IL1RA, IL1RN, Interleukin-1 receptor antagonist protein, IL-1RN, IL-1ra, IRAP, ICIL-1RA, IL1 inhibitor, Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10, IL8, CXCL8, Interleukin-8, IL-8, C-X-C motif chemokine 8, Chemokine (C-X-C motif) ligand 8, Emoctakin, Granulocyte chemotactic protein 1, Monocyte-derived neutrophil chemotactic factor, Monocyte-derived neutrophil-activating peptide, Neutrophil-activating protein 1, Protein 3-10C, T-cell chemotactic factor, GCP-1, MDNCF, MONAP, NAP-1, OB, OBS, LEP, Leptin, Obese protein, Obesity factor, D3F15S2, DNF15S2, HGFL, MST1, Hepatocyte growth factor-like protein, Macrophage stimulatory protein, Macrophage-stimulating protein, MSP, OCIF, OPG, TNFRSF11B, Tumor necrosis factor receptor superfamily member 11B, Osteoclastogenesis inhibitory factor, Osteoprotegerin, VEGF, VEGFA, L-VEGF, Vascular permeability factor, VPF, ARP4, HFARP, PGAR, PP1158, PSEC0166, UNQ171/PRO197, ANGPTL4, Angiopoietin-related protein 4, Angiopoietin-like protein 4, Hepatic fibrinogen/angiopoietin-related protein, Somatotropin, Growth hormone, Growth hormone 1, Pituitary growth hormone, GH, GH-N, GH1, NKSF1, IL12A, Interleukin-12 subunit alpha, IL-12A, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, NK cell stimulatory factor chain 1, CLMF p35, CD120b, TNFBR, TNFR2, TNFRSF1B, Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor 2, Tumor necrosis factor receptor type II, p75, p80 TNF-alpha receptor, TNF-R2, TNF-RII, TNFR-II, TIG2, RARRES2, Retinoic acid receptor responder protein 2, Chemerin, RAR-responsive protein TIG2, Tazarotene-induced gene 2 protein, FIZZ3, HXCP1, RSTN, UNQ407/PRO1199, RETN, Resistin, Adipose tissue-specific secretory factor, C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein, Cysteine-rich secreted protein A12-alpha-like 2, Cysteine-rich secreted protein FIZZ3, ADSF, Serum amyloid A-1 protein, SAA, SAA1, TSP, TSP1, THBS1, Thrombospondin-1, Glycoprotein G, Pepsin A-3, Pepsinogen-3, PGA3

3 Images
Multiplex Protein Detection - Human Obesity Antibody Array (40 Targets) - Quantitative (AB197417)
  • Multiplex Protein Detection

Supplier Data

Multiplex Protein Detection - Human Obesity Antibody Array (40 Targets) - Quantitative (AB197417)

Standard Curve obtained with Abcam Human Obesity Antibody Array - Quantitative (40 targets) (ab197417).

Multiplex Protein Detection - Human Obesity Antibody Array (40 Targets) - Quantitative (AB197417)
  • Multiplex Protein Detection

Supplier Data

Multiplex Protein Detection - Human Obesity Antibody Array (40 Targets) - Quantitative (AB197417)

Array Map for Human Obesity Antibody Array - Quantitative (40 targets) (ab197417).

Multiplex Protein Detection - Human Obesity Antibody Array (40 Targets) - Quantitative (AB197417)
  • Multiplex Protein Detection

Supplier Data

Multiplex Protein Detection - Human Obesity Antibody Array (40 Targets) - Quantitative (AB197417)

Assay Summary for Human Obesity Antibody Array - Quantitative (40 targets) (ab197417).

Key facts

Reacts with

Human

Target

CFD

target

Detection method

Fluorescent

Assay Platform

Multiplex

Sample types

Tissue Lysate, Plasma, Cell culture media, Serum, Other biological fluids, Cell Lysate

Product details

ab197417 can be used for quantitative measurement of 40 Human cytokines. Suitable for serum, plasma, cell culture media, other body fluids, cell and tissue lysates.
Targets names: Adiponectin, Adipsin, AgRP, ANGPTL4, BDNF, Chemerin, CRP, GH, IFNγ, IGFBP-1, IGFBP-2, IGF-I, IL-10, IL-12p40, IL-12p70, IL-1β, IL-1ra, IL-6, IL-8, Insulin, Leptin, Lipocalin-2, MSPα, OPG, PAI-1, PDGF-BB, Pepsinogen 1, Pepsinogen 2, Procalcitonin, Prolactin, RANTES, RBP4, Resistin, SAA, TGFβ1, TSP-1, TNF RI, TNF RII, TNFα and VEGF.

Quantitative antibody arrays can be used to quantitate up to 40 cytokines with as little as 50 µL of sample. Arrays are available for 400 human and 200 mouse proteins. Each glass slide is spotted with 16 identical antibody arrays and is provided with a 16 well gasket to allow separate samples to be applied to each array. Each antibody is spotted in quadruplicate in each array. For reproducible quantitation, eight of the arrays are used with a cocktail of protein standards to produce a standard curve. The same 8 standard curve arrays can be used across multiple slides, allowing measurement of 22 experimental samples with 2 slides, 36 with 3 slides etc. For high through-put, 4 slides can be nested into a tray matching a standard microplate, allowing automated processing with a liquid handling workstation. Array processing can be completed within one working day (see workflow diagram below) and arrays can be analyzed with a wide number of laser glass slide array / gene microarray scanners. If you don’t have a suitable scanner then we recommend our membrane antibody arrays, which can be analyzed with any WB chemiluminescent reader. The standard is affinity purified. "

What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Product protocols

Target data

Serine protease that initiates the alternative pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 21205667, PubMed : 22362762, PubMed : 6769474, PubMed : 874324, PubMed : 9748277). In contrast to other complement pathways (classical, lectin and GZMK) that are directly activated by pathogens or antigen-antibody complexes, the alternative complement pathway is initiated by the spontaneous hydrolysis of complement C3 (PubMed : 21205667, PubMed : 22362762, PubMed : 6769474, PubMed : 874324). The alternative complement pathway acts as an amplification loop that enhances complement activation by mediating the formation of C3 and C5 convertases (PubMed : 21205667, PubMed : 22362762, PubMed : 6769474, PubMed : 874324). Activated CFD cleaves factor B (CFB) when the latter is complexed with complement C3b, activating the C3 convertase of the alternative pathway (PubMed : 21205667, PubMed : 6769474, PubMed : 874324, PubMed : 9748277).
See full target information CFD

Additional targets

PGA3,AGRP,ANGPTL4,BDNF,RARRES2,CRP,GH1,IFNG,IGF1,IGFBP1,IGFBP2,IL1B,IL1RN,IL10,IL12B,IL12A,IL6,CXCL8,INS,LEP,LCN2,MST1,TNFRSF11B,SERPINE1,PDGFB,PGC,CALCA,PRL,CCL5,RBP4,RETN,SAA1,TGFB1,THBS1,TNF,TNFRSF1A,TNFRSF1B,VEGFA
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