Abcam's Human Obesity Antibody Array - Membrane (62 targets) detects 62 Human Adipokines in 4 or 8 samples.
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- Food & function 14:1349-13562023Anti-obesity effect of vegetable juice fermented with lactic acid bacteria isolated from kimchi in C57BL/6J mice and human mesenchymal stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMoeun Lee,Ye-Rang Yun,Eun Ji Choi,Jung Hee Song,Jin Yong Kang,Daun Kim,Ki Won Lee,Ji Yoon ChangPubMed 36630124
- Menopause (New York, N.Y.) 25:211-2162017Resistance training reduces inflammation and fatigue and improves physical function in older breast cancer survivors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMonica C Serra,Alice S Ryan,Heidi K Ortmeyer,Odessa Addison,Andrew P GoldbergPubMed 28832427
- Frontiers in neuroscience 11:2782017Plasma Extracellular Vesicles Enriched for Neuronal Origin: A Potential Window into Brain Pathologic Processes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaja Mustapic et. al.PubMed 28588440
- Breast cancer research and treatment 150:685-952015Targeting exosomes from preadipocytes inhibits preadipocyte to cancer stem cell signaling in early-stage breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRamkishore Gernapudi et. al.PubMed 25783182
Key facts
- Assay type
- Semi-quantitative
- Reactive species
- Human
- Target
- TNFRSF9
(See target data below)
Target data
Function
Receptor for TNFSF9/4-1BBL. Conveys a signal that enhances CD8(+) T-cell survival, cytotoxicity, and mitochondrial activity, thereby promoting immunity against viruses and tumors (Probable).
Additional Targets
ACE2, CFD, AGRP, ANGPT1, ANGPT2, ANGPTL4, CRP, CXCL5, FAS, FGF6, GH1, CCL16, IFNG, IGF1, IGF1R, IGFBP1, IGFBP2, IGFBP3, IL1A, IL1B, IL1R1, IL1RL1, IL10, Interleukin-11, IL12A, IL6, IL6R, CXCL8, INS, CXCL10, LEP, LEPR, LIF, XCL1, CCL2, CCL7, CSF1, MIF, CCL4, MST1, OSM, TNFRSF11B, SERPINE1, CCL18, PDGFA, PDGFB, PDGFB, CCL5, RETN, SAA1, CXCL12, CCL25, TGFB1, TIMP1, TIMP2, TNF, TNFRSF1A, TNFRSF1B, VEGFA, EDA2R
Alternative names
CD137, ILA, TNFRSF9, Tumor necrosis factor receptor superfamily member 9, 4-1BB ligand receptor, CDw137, T-cell antigen 4-1BB homolog, T-cell antigen ILA
What's included?
Recommended products
Abcam's Human Obesity Antibody Array - Membrane (62 targets) detects 62 Human Adipokines in 4 or 8 samples.
Key facts
- Assay type
- Semi-quantitative
- Reactive species
- Human
- Target
- TNFRSF9
- Applications
- Multiplex Protein Detection
- Sample types
- Saliva, Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture media, Cell culture supernatant, Milk, Serum, Other biological fluids, Whole Blood, Cell Lysate
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Abcam's Human Obesity Antibody Array - Membrane (62 targets) detects 62 Human Adipokines in 4 or 8 samples. Suitable for all liquid sample types.
Targets:4-1BB, ACE-2, Adiponectin, Adipsin, AgRP, Angiopoietin 1, Angiopoietin 2, ANGPTL4, CRP, ENA-78, Fas, FGF-6, Growth Hormone, HCC-4, IFN-gamma, IGFBP-1, IGFBP-2, IGFBP-3, IGF-1, IGF-1 sR, IL-1 R4, IL-1 sRI, IL-10, IL-11, IL-12, IL-1 alpha, IL-1 beta, IL-6, IL-6 sR, IL-8, Insulin, IP-10, Leptin R, Leptin, LIF, Lymphotactin, MCP-1, MCP-3, M-CSF, MIF, MIP-1 beta, MSP-alpha, Osteoprotegerin, OSM , PAI-I, PARC, PDGF-AA, PDGF-AB, PDGF-BB, RANTES, Resistin, Serum Amyloid A , SDF-1 , sTNFRII, sTNFRI, TECK, TGF beta 1, TIMP-1, TIMP-2, TNF alpha, VEGF-A, XEDAR
If you are interested in this cytokine array, arrays ab133998 and ab134000 may also be of interest. A list of human antibody membrane arrays is available ."
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2 product images
This image is courtesy of an anonymous customer reviewMultiplex Protein Detection - Human Obesity Antibody Array - Membrane (62 targets) (ab169819)
5 mg of lyophilized microdissected skeletal muscle (vastus laterals) was homogenized in 180 uL 1X cell lysis buffer (included in kit). After a 10,000 x g 5 min spin at 4°C removed the supernatant was removed and the total protein determined. 250 μg of total protein was added to each membrane with overnight incubations for each incubation step. The attached image is the first blot setting up the method and was exposed using a FluorChem M for 27 seconds. As can be seen Acrp30 will require less protein/less exposure but otherwise all but seven cytokines were visualized following this protocol.
Abreview rating 5/5 stars. Review from Abcam user community. Verified customer at Baltimore VA Medical Center. Submitted Oct 9 2014.
Multiplex Protein Detection - Human Obesity Antibody Array - Membrane (62 targets) (ab169819)
Typical images obtained with Abcam Human Cytokine Antibody Array. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at room temperature for 1 minute.
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