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ab193662 can be used for the simultaneous detection of 71 phosphorylated Human receptor tyrosine kinases (RTKs) in cell and tissue lysates.

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Multiplex Protein Detection - Human RTK Phosphorylation Antibody Array -Membrane (71 Targets) (AB193662), expandable thumbnail

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Key facts

Assay type
Semi-quantitative
Reactive species
Human
Target
EPHB4
(See target data below)

Target data

Function

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 it is involved in the regulation of cell adhesion and migration, and plays a central role in heart morphogenesis, angiogenesis and blood vessel remodeling and permeability. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells.

Additional Targets

ACVRL1, AXL, Tyrosine-protein kinase Blk, BMX, ABL1, Tyrosine-protein kinase CSK, EGFR, EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHB1, EPHB2, EPHB3, EPHB4, EPHB6, ERBB2, ERBB3, PTK2, FER, FGFR1, FGFR1, FGFR2, FGR, FRK, Tyrosine-protein kinase Fyn, HCK, IGF1R, INSR, ITK, JAK3, LCK, LTK, LYN phospho Y418, MATK, CSF1R, Muscle, skeletal receptor tyrosine-protein kinase, NGFR, PDGFRA phospho Y572 + Y574, PDGFRB, PTK2B, RET, ROR1, ROR2, RYK, KIT, SRMS, SYK, TEC, TIE1, TEK, TNK1, NTRK2, TXK, TYK2, KDR, FLT4

Alternative names

HTK, MYK1, TYRO11, EPHB4, Ephrin type-B receptor 4, Hepatoma transmembrane kinase, Tyrosine-protein kinase TYRO11

What's included?

1 x 8 Membranes
Components
1,000X HRP-Conjugated Streptavidin
1 x 50 µL
1X Blocking Buffer
2 x 25 mL
20X Wash Buffer I
2 x 30 mL
20X Wash Buffer II
2 x 30 mL
2X Cell Lysis Buffer
1 x 16 mL
8-Well Incubation Tray (with Lid)
1 x 1 Unit
Biotinylated Anti-Phosphotyrosine Antibody (C1)
4 x 1 Vial
Detection Buffer C
1 x 2.5 mL
Detection Buffer D
1 x 2.5 mL
Human RTK Antibody Array Membranes (C1)
2 x 4 Unit
Phosphatase Inhibitor Cocktail Set II
2 x 1 Vial
Plastic sheets
1 x 1 Unit
Protease Inhibitor Cocktail
2 x 1 Unit

Recommended products

ab193662 can be used for the simultaneous detection of 71 phosphorylated Human receptor tyrosine kinases (RTKs) in cell and tissue lysates.

Key facts

Assay type
Semi-quantitative
Reactive species
Human
Target
EPHB4
Applications
Multiplex Protein Detection
Sample types
Tissue Lysate, Cell Lysate

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

ab193662 can be used for the simultaneous detection of 71 phosphorylated Human receptor tyrosine kinases (RTKs) in cell and tissue lysates.

Targets: ALB1, ACK1, ALK, Axl, Blk, BMX, Btk, Csk, Dtk, EGFR, EphA1, EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, EphA8, EphB1, EphB2, EphB3, EphB4, EphB6, ErbB2, ErbB3, ErbB4, FAK, FER, FGFR1, FGFR2, FGFR2 (α isoform), Fgr, FRK, Fyn, Hck, HGFR, IGF-I R, Insulin R (CD220), Itk, JAK1, JAK2, JAK3, LCK, LTK, Lyn, MATK, M-CSFR, MUSK, NGFR (TNFRSF16), PDGFR-α, PDGFR-β, PYK2, RET, ROR1, ROR2, ROS, RYK, SCF R (CD117/c-kit), SRMS, SYK, Tec, Tie-1, Tie-2, TNK1, TRKB, TXK, Tyk2, TYRO10 (DDR2/TKT), VEGFR2, VEGFR3, ZAP70.


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1 product image

  • Multiplex Protein Detection - Human RTK Phosphorylation Antibody Array -Membrane (71 Targets) (ab193662), expandable thumbnail

    Multiplex Protein Detection - Human RTK Phosphorylation Antibody Array -Membrane (71 Targets) (ab193662)

    Typical images obtained with Abcam Human RTK Phosphorylation Antibody Array -Membrane (71 Targets) ab193662. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at room temperature for 1 minute.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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