Skip to main content

ab193662 can be used for the simultaneous detection of 71 phosphorylated Human receptor tyrosine kinases (RTKs) in cell and tissue lysates.

Be the first to review this product! Submit a review

Images

Key facts

Assay type

Semi-quantitative

Reactive species

Human

Target

TNK2

Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...

Target data

Function

Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. May be involved both in adult synaptic function and plasticity and in brain development. Activates AKT1 by phosphorylating it on 'Tyr-176'. Phosphorylates AR on 'Tyr-267' and 'Tyr-363' thereby promoting its recruitment to androgen-responsive enhancers (AREs). Phosphorylates WWOX on 'Tyr-287'. Phosphorylates MCF2, thereby enhancing its activity as a guanine nucleotide exchange factor (GEF) toward Rho family proteins. Contributes to the control of AXL receptor levels. Confers metastatic properties on cancer cells and promotes tumor growth by negatively regulating tumor suppressor such as WWOX and positively regulating pro-survival factors such as AKT1 and AR. Phosphorylates WASP (PubMed:20110370).

Alternative names

What's included?

1 x 8 Membranes
Components
1,000X HRP-Conjugated Streptavidin
1 x 50 µL
1X Blocking Buffer
2 x 25 mL
20X Wash Buffer I
2 x 30 mL
20X Wash Buffer II
2 x 30 mL
2X Cell Lysis Buffer
1 x 16 mL
8-Well Incubation Tray (with Lid)
1 x 1 Unit
Biotinylated Anti-Phosphotyrosine Antibody (C1)
4 x 1 Vial
Detection Buffer C
1 x 2.5 mL
Detection Buffer D
1 x 2.5 mL
Human RTK Antibody Array Membranes (C1)
2 x 4 Unit
Phosphatase Inhibitor Cocktail Set II
2 x 1 Vial
Plastic sheets
1 x 1 Unit
Protease Inhibitor Cocktail
2 x 1 Unit

Recommended products

  1. Loading...
  2. Loading...
  3. Loading...
  4. Loading...

ab193662 can be used for the simultaneous detection of 71 phosphorylated Human receptor tyrosine kinases (RTKs) in cell and tissue lysates.

Alternative names

Key facts

Assay type

Semi-quantitative

Reactive species

Human

Target

TNK2

Applications

Multiplex Protein Detection

Sample types

Tissue Lysate, Cell Lysate

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

ab193662 can be used for the simultaneous detection of 71 phosphorylated Human receptor tyrosine kinases (RTKs) in cell and tissue lysates.

Targets: ALB1, ACK1, ALK, Axl, Blk, BMX, Btk, Csk, Dtk, EGFR, EphA1, EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, EphA8, EphB1, EphB2, EphB3, EphB4, EphB6, ErbB2, ErbB3, ErbB4, FAK, FER, FGFR1, FGFR2, FGFR2 (α isoform), Fgr, FRK, Fyn, Hck, HGFR, IGF-I R, Insulin R (CD220), Itk, JAK1, JAK2, JAK3, LCK, LTK, Lyn, MATK, M-CSFR, MUSK, NGFR (TNFRSF16), PDGFR-α, PDGFR-β, PYK2, RET, ROR1, ROR2, ROS, RYK, SCF R (CD117/c-kit), SRMS, SYK, Tec, Tie-1, Tie-2, TNK1, TRKB, TXK, Tyk2, TYRO10 (DDR2/TKT), VEGFR2, VEGFR3, ZAP70.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Multiplex Protein Detection - Human RTK Phosphorylation Antibody Array -Membrane (71 Targets) (ab193662), expandable thumbnail

    Multiplex Protein Detection - Human RTK Phosphorylation Antibody Array -Membrane (71 Targets) (ab193662)

    Typical images obtained with Abcam Human RTK Phosphorylation Antibody Array -Membrane (71 Targets) ab193662. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at room temperature for 1 minute.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

There was a problem

We can’t download that datasheet. Please try again. If you need help, contact our Customer Services team at technical@abcam.com