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AB169809

Human Th1/Th2/Th17 Antibody Array - Membrane (34 targets)

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(2 Publications)

ab169809 is for the simultaneous detection of 34 Human Th1, Th2, and Th17 cytokines.

View Alternative Names

CD30, D1S166E, TNFRSF8, Tumor necrosis factor receptor superfamily member 8, CD30L receptor, Ki-1 antigen, Lymphocyte activation antigen CD30, CD121a, IL1R, IL1RA, IL1RT1, IL1R1, Interleukin-1 receptor type 1, IL-1R-1, IL-1RT-1, IL-1RT1, CD121 antigen-like family member A, Interleukin-1 receptor alpha, Interleukin-1 receptor type I, p80, IL-1R-alpha, CD40, TNFRSF5, Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40, CD130, Interleukin-6 receptor subunit beta, IL-6 receptor subunit beta, IL-6R subunit beta, IL-6R-beta, IL-6RB, CDw130, Interleukin-6 signal transducer, Membrane glycoprotein 130, Oncostatin-M receptor subunit alpha, gp130, IL6ST, CD126, Interleukin-6 receptor subunit alpha, IL-6 receptor subunit alpha, IL-6R subunit alpha, IL-6R-alpha, IL-6RA, IL-6R 1, Membrane glycoprotein 80, gp80, IL6R, IFNB2, IL6, Interleukin-6, IL-6, B-cell stimulatory factor 2, CTL differentiation factor, Hybridoma growth factor, Interferon beta-2, BSF-2, CDF, IFN-beta-2, C17orf33, GCSF, CSF3, Granulocyte colony-stimulating factor, G-CSF, Pluripoietin, CD217, IL17R, IL17RA, Interleukin-17 receptor A, IL-17 receptor A, IL-17RA, CDw217, TGFB, TGFB1, Transforming growth factor beta-1 proprotein, Transforming growth factor beta-3 proprotein, TGFB3, CD357, AITR, GITR, UNQ319/PRO364, TNFRSF18, Tumor necrosis factor receptor superfamily member 18, Activation-inducible TNFR family receptor, Glucocorticoid-induced TNFR-related protein, ILTIF, ZCYTO18, UNQ3099/PRO10096, IL22, Interleukin-22, IL-22, Cytokine Zcyto18, IL-10-related T-cell-derived-inducible factor, IL-TIF, CD121b, IL1RB, IL1R2, Interleukin-1 receptor type 2, IL-1R-2, IL-1RT-2, IL-1RT2, CD121 antigen-like family member B, CDw121b, IL-1 type II receptor, Interleukin-1 receptor beta, Interleukin-1 receptor type II, IL-1R-beta, CTLA8, IL17, IL17A, Interleukin-17A, IL-17, IL-17A, Cytotoxic T-lymphocyte-associated antigen 8, CTLA-8, NKSF2, IL12B, Interleukin-12 subunit beta, IL-12B, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p40, NK cell stimulatory factor chain 2, CLMF p40, Interleukin-2, IL-2, T-cell growth factor, TCGF, IL2, Interleukin-4, IL-4, B-cell stimulatory factor 1, Binetrakin, Lymphocyte stimulatory factor 1, Pitrakinra, BSF-1, IL4, IL1F2, IL1B, Interleukin-1 beta, IL-1 beta, Catabolin, TNFA, TNFSF2, TNF, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Interleukin-21, IL-21, Za11, IL21, GMCSF, CSF2, Granulocyte-macrophage colony-stimulating factor, GM-CSF, Colony-stimulating factor, Molgramostin, Sargramostim, CSF, Interferon gamma, IFN-gamma, Immune interferon, IFNG, Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10, NC30, IL13, Interleukin-13, IL-13, Interleukin-5, IL-5, B-cell differentiation factor I, Eosinophil differentiation factor, T-cell replacing factor, TRF, IL5, LARC, MIP3A, SCYA20, CCL20, C-C motif chemokine 20, Beta-chemokine exodus-1, CC chemokine LARC, Liver and activation-regulated chemokine, Macrophage inflammatory protein 3 alpha, Small-inducible cytokine A20, MIP-3-alpha, TNFB, TNFSF1, LTA, Lymphotoxin-alpha, LT-alpha, TNF-beta, Tumor necrosis factor ligand superfamily member 1, CD254, OPGL, RANKL, TRANCE, TNFSF11, Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, Osteoprotegerin ligand, Receptor activator of nuclear factor kappa-B ligand, TNF-related activation-induced cytokine, ODF, CD154, CD40L, TNFSF5, TRAP, CD40LG, CD40 ligand, CD40-L, T-cell antigen Gp39, TNF-related activation protein, Tumor necrosis factor ligand superfamily member 5, NKSF1, IL12A, Interleukin-12 subunit alpha, IL-12A, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, NK cell stimulatory factor chain 1, CLMF p35, CD360, NILR, UNQ3121/PRO10273, IL21R, Interleukin-21 receptor, IL-21 receptor, IL-21R, Novel interleukin receptor, Interleukin-17F, IL-17F, Cytokine ML-1, IL17F, IL28A, ZCYTO20, IFNL2, Interferon lambda-2, IFN-lambda-2, Cytokine Zcyto20, Interleukin-28A, IL-28A

3 Images
ELISA - Human Th1/Th2/Th17 Antibody Array - Membrane (34 targets) (AB169809)
  • ELISA

Unknown

ELISA - Human Th1/Th2/Th17 Antibody Array - Membrane (34 targets) (AB169809)

PBMC.

Human peripheral blood cells (1x106 cells/mL) were cultured in RPMI media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 mg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with 10 mg/mL PHA. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab169809. Mean pixel density was quantified using CCD camera software analysis.

ELISA - Human Th1/Th2/Th17 Antibody Array - Membrane (34 targets) (AB169809)
  • ELISA

Unknown

ELISA - Human Th1/Th2/Th17 Antibody Array - Membrane (34 targets) (AB169809)

Human serum and plasma (EDTA) from a pooled donor (n=50) sample was diluted to 25% and assayed using ab169809. Mean pixel density was quantified using CCD camera software analysis.

Multiplex Protein Detection - Human Th1/Th2/Th17 Antibody Array - Membrane (34 targets) (AB169809)
  • Multiplex Protein Detection

Unknown

Multiplex Protein Detection - Human Th1/Th2/Th17 Antibody Array - Membrane (34 targets) (AB169809)

Typical results obtained with Abcam Membrane Antibody Arrays. Image above presents typical images obtained with Abcam Human Cytokine Membrane Antibody Array. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at RT for 1 min.

Key facts

Reacts with

Human

Target

TNFRSF8

target

Applications

Multiplex Protein Detection

applications

Sample types

Saliva, Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture media, Cell culture supernatant, Milk, Serum, Other biological fluids, Whole Blood, Cell Lysate

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Multiplex Protein Detection": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Product details

ab169809 is for the simultaneous detection of 34 Human Th1, Th2, and Th17 cytokines. Suitable for all liquid sample types.

Targets: CD30, CD40 Ligand, CD40, G-CSF, GITR, GM-CSF, IFN-gamma, IL-1 beta, IL-1 sRI, IL-1 sRII, IL-2, IL-4, IL-5, IL-6, IL-6 R, IL-10, IL-12 p40, IL-12 p70, IL-13, IL-17, IL-17F, IL-17 R, IL-21, IL-21R, IL-22, IL-23 (p19), IL-28A, MIP-3 alpha, spg130, TGF beta 1, TGF beta 3, TNF alpha, TNF beta, TRANCE.

Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.

A table listing all of our human membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Product protocols

Target data

Receptor for TNFSF8/CD30L (PubMed : 8391931). May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B (PubMed : 8999898).
See full target information TNFRSF8

Additional targets

CD40,CD40LG,IL10,IL12B,IL12A,IL13,IL17RA,IL17A,IL17F,IL2,IL21,IL21R,CSF3,IL22,IFNL2,IL4,IL5,IL6,IL6R,CCL20,TGFB1,TGFB3,TNFRSF18,TNF,LTA,TNFSF11,CSF2,IL6ST,IFNG,IL1B,IL1R1,IL1R2

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed35682603

2022

Human Omental Mesothelial Cells Impart an Immunomodulatory Landscape Impeding B- and T-Cell Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Benoit R Gauthier,Diana Rubio-Contreras,Juan Carlos Gómez-Rosado,Luis Cristobal Capitán-Morales,Abdelkrim Hmadcha,Bernat Soria,Christian Claude Lachaud

International journal of molecular sciences 23: PubMed35328606

2022

Reduced Expression of PD-1 in Circulating CD4+ and CD8+ Tregs Is an Early Feature of RRMS.

Applications

Unspecified application

Species

Unspecified reactive species

Maja Machcińska,Magdalena Kierasińska,Martyna Michniowska,Marta Maruszewska-Cheruiyot,Ludmiła Szewczak,Rafał Rola,Anna Karlińska,Michael Stear,Katarzyna Donskow-Łysoniewska
View all publications
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