Skip to main content

ab197460 can be used for quantitative measurement of 80 Mouse cytokines.

Be the first to review this product! Submit a review

Key facts

Assay type
Quantitative
Reactive species
Mouse
Target
Areg
(See target data below)
Assay Platform
Multiplex

Target data

Function

Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.

Additional Targets

Csf2rb, Vegfd, Il21, Pro-epidermal growth factor, Tnfrsf11b, Tnfrsf1b, Ccl3, Ccl1, Spp1, Il10, Il15, Hepatocyte growth factor, Cxcl16, Ccl9, Il17a, Il2ra, Tnf, Il2, Mmp9, Igfbp6, Cd40, Tnfrsf18, Ccl12, Ccl2, Ccl17, Vegfd, Il21, Pro-epidermal growth factor, Tnfrsf11b, Tnfrsf1b, Ccl3, Ccl1, Spp1, Il10, Il15, Hepatocyte growth factor, Cxcl16, Ccl9, Il17a, Il2ra, Tnf, Il2, Mmp9, Igfbp6, Cd40, Tnfrsf18, Ccl12, Ccl2, Ccl17, Axl, Ccl11, Ccl20, Ccl22, Ccl24, Ccl5, CD70, Csf1, Csf3, Cx3cl1, Cxcl1, Cxcl11, Cxcl12, Cxcl13, Cxcl2, Cxcl5, Cxcl9, Faslg, Fgf2, Icam1, Ifng, Ifnl2, Igf1, Igfbp2, Igfbp3, Igfbp5, Il12a, Il12b, Il13, Il17f, IL1A_MOUSE, Il1b, Il1rn, Il20, Il23a, IL-25, Il3, Il4, Il5, Il6, Il7, Kitlg, Lep, Pf4, Prl, Retn, Sele, Selp, Tnfrsf1a, Tnfrsf8, Tnfsf8, Thpo, Vcam1, Vegfa

Alternative names

What's included?

8 Test
Components
20X Wash Buffer I
4 x 30 mL
20X Wash Buffer II
2 x 30 mL
Adhesive Film
2 x 1 Unit
Cy3 equivalent dye-conjugated Streptavidin
2 x 5 µL
Mouse Cytokine Array A (40 T) Biotinylated Antibody Cocktail
1 x 25 µL
Mouse Cytokine Array A (40 T) Glass Slide
1 x 1 Slide
Mouse Cytokine Array A (40 T) Lyophilized Cytokine Standard Mix
1 x 1 Vial
Mouse Cytokine Array B (40 T) Biotinylated Antibody Cocktail
1 x 25 µL
Mouse Cytokine Array B (40 T) Glass Slide
1 x 1 Slide
Mouse Cytokine Array B (40 T) Lyophilized Cytokine Standard Mix
1 x 1 Vial
Sample Diluent
2 x 15 mL
Slide Washer/Dryer (30 mL Centrifuge Tube)
2 x 1 Unit

Recommended products

ab197460 can be used for quantitative measurement of 80 Mouse cytokines.

Key facts

Assay type
Quantitative
Reactive species
Mouse
Target
Areg
Detection method
Fluorescent
Assay Platform
Multiplex
Sample types
Tissue Lysate, Plasma, Cell culture media, Serum, Other biological fluids, Cell Lysate

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

ab197460 can be used for quantitative measurement of 80 Mouse cytokines. Suitable for serum, plasma, cell culture media, other body fluids, cell and tissue lysates.
Targets names:

Array A: Axl, CD27L, CD30T, CD40, CXCL16, EGF, Eselectin, Fractalkine, GITR, HGF, IGFBP-2, IGFBP-3, IGFBP-5, IGFBP-6, IGF-I, IL-12p70, IL-17E, IL-17F, IL-1ra, IL-2 Rα, IL-20, IL-23, IL-28, I-TAC, MDC, MIP-2, MIP-3α, OPN, OPG, Prolactin, Pro-MMP-9, Pselectin, Resistin, SCF, SDF-1α, TPO, VCAM-1, VEGF, VEGF-D.

Array B: bFGF, BLC, CD30L, Eotaxin, Eotaxin-2, Fas L, GCSF, GM-CSF, ICAM-1, IFNγ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-10, IL-12p40, IL-13, IL-15, IL-17, IL-21, KC, Leptin, LIX, MCP-1, MCP-5, M-CSF, MIG, MIP-1α, MIP-1γ, PF-4, RANTES, TARC, TCA-3, TNFα, TNFRI, TNFRII.

The analytes measured by this kit are split accross 2 different arrays on different slides, both of which are provided in the kit.


Quantitative antibody arrays can be used to quantitate up to 40 cytokines with as little as 50 µL of sample. Arrays are available for 400 human and 200 mouse proteins. Each glass slide is spotted with 16 identical antibody arrays and is provided with a 16 well gasket to allow separate samples to be applied to each array. Each antibody is spotted in quadruplicate in each array. For reproducible quantitation, eight of the arrays are used with a cocktail of protein standards to produce a standard curve. The same 8 standard curve arrays can be used across multiple slides, allowing measurement of 22 experimental samples with 2 slides, 36 with 3 slides etc. For high through-put, 4 slides can be nested into a tray matching a standard microplate, allowing automated processing with a liquid handling workstation. Array processing can be completed within one working day (see workflow diagram below) and arrays can be analyzed with a wide number of laser glass slide array / gene microarray scanners. If you don’t have a suitable scanner then we recommend our membrane antibody arrays, which can be analyzed with any WB chemiluminescent reader. The standard is affinity purified. "

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

0 product images

    Downloads

    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

    For licensing inquiries, please contact partnerships@abcam.com