JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB197465

Mouse Cytokine Antibody Array A (20 Targets) - Quantitative

Be the first to review this product! Submit a review

|

(5 Publications)

ab197465 can be used for quantitative measurement of 20 Mouse cytokines.

View Alternative Names

Scyb5, Cxcl5, C-X-C motif chemokine 5, Cytokine LIX, Small-inducible cytokine B5, CD131, Aic2b, Csf2rb1, Il3rb1, Csf2rb, Cytokine receptor common subunit beta, GM-CSF/IL-3/IL-5 receptor common beta subunit, Gro, Gro1, Mgsa, Scyb1, Cxcl1, Growth-regulated alpha protein, C-X-C motif chemokine 1, Platelet-derived growth factor-inducible protein KC, Secretory protein N51, Interferon gamma, IFN-gamma, Ifng, Interleukin-12 subunit alpha, IL-12A, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, CLMF p35, Il12a, Il-13, Il13, Interleukin-13, IL-13, T-cell activation protein P600, Csfmu, Il-3, Il3, Interleukin-3, IL-3, Hematopoietic growth factor, Mast cell growth factor, Multipotential colony-stimulating factor, P-cell-stimulating factor, MCGF, Il-4, Il4, Interleukin-4, IL-4, B-cell IgG differentiation factor, B-cell growth factor 1, B-cell stimulatory factor 1, IGG1 induction factor, Lymphocyte stimulatory factor 1, BSF-1, Il-5, Il5, Interleukin-5, IL-5, B-cell growth factor II, Cytotoxic T-lymphocyte inducer, Eosinophil differentiation factor, T-cell replacing factor, BCGF-II, TRF, Il-6, Il6, Interleukin-6, IL-6, B-cell hybridoma growth factor, Interleukin HP-1, Interleukin-9, IL-9, Cytokine P40, T-cell growth factor P40, Il9, Vegf, Vegfa, L-VEGF, Vascular permeability factor, VPF, Mig, Scyb9, Cxcl9, C-X-C motif chemokine 9, Gamma-interferon-induced monokine, Monokine induced by interferon-gamma, Protein m119, Small-inducible cytokine B9, MIG, MuMIG, Ctla8, Il17, Il17a, Interleukin-17A, IL-17, IL-17A, Cytotoxic T-lymphocyte-associated antigen 8, CTLA-8, Interleukin-1 beta, IL-1 beta, Il1b, Tnfa, Tnfsf2, Tnf, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Interleukin-1 alpha, IL-1 alpha, Il1a, Il-10, Il10, Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, Il-2, Il2, Interleukin-2, IL-2, T-cell growth factor, TCGF, Csfm, Csf1, Macrophage colony-stimulating factor 1, CSF-1, MCSF, Proteoglycan macrophage colony-stimulating factor, PG-M-CSF

2 Images
Multiplex Protein Detection - Mouse Cytokine Antibody Array A (20 Targets) - Quantitative (AB197465)
  • Multiplex Protein Detection

Supplier Data

Multiplex Protein Detection - Mouse Cytokine Antibody Array A (20 Targets) - Quantitative (AB197465)

Mouse Cytokine Antibody Array A - Quantitative (20 targets) Array Map (ab197465)

Multiplex Protein Detection - Mouse Cytokine Antibody Array A (20 Targets) - Quantitative (AB197465)
  • Multiplex Protein Detection

Supplier Data

Multiplex Protein Detection - Mouse Cytokine Antibody Array A (20 Targets) - Quantitative (AB197465)

Mouse Cytokine Antibody Array A - Quantitative (20 targets) Standard Curve (ab197465)

Key facts

Reacts with

Mouse

Target

Cxcl5

target

Detection method

Fluorescent

Assay Platform

Multiplex

Sample types

Tissue Lysate, Plasma, Cell culture media, Serum, Other biological fluids, Cell Lysate

Product details

ab197465 can be used for quantitative measurement of 20 Mouse cytokines. Suitable for serum, plasma, cell culture media, other body fluids, cell and tissue lysates.
Targets names: GM-CSF, IFNγ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12, IL-13, IL-17, KC, MCP-1, MCSF, RANTES, TNFα, VEGF.

Quantitative antibody arrays can be used to quantitate up to 40 cytokines with as little as 50 µL of sample. Arrays are available for 400 human and 200 mouse proteins. Each glass slide is spotted with 16 identical antibody arrays and is provided with a 16 well gasket to allow separate samples to be applied to each array. Each antibody is spotted in quadruplicate in each array. For reproducible quantitation, eight of the arrays are used with a cocktail of protein standards to produce a standard curve. The same 8 standard curve arrays can be used across multiple slides, allowing measurement of 22 experimental samples with 2 slides, 36 with 3 slides etc. For high through-put, 4 slides can be nested into a tray matching a standard microplate, allowing automated processing with a liquid handling workstation. Array processing can be completed within one working day (see workflow diagram below) and arrays can be analyzed with a wide number of laser glass slide array / gene microarray scanners. If you don’t have a suitable scanner then we recommend our membrane antibody arrays, which can be analyzed with any WB chemiluminescent reader. The standard is affinity purified. "

What's included?

{ "values": { "50Test": { "sellingSize": "50 Test", "publicAssetCode":"ab197465-50Test", "assetComponentDetails": [ { "size":"4 x 1 Unit", "name":"Adhesive Film", "number":"AB197465-CMP09" }, { "size":"4 x 25 µL", "name":"Mouse Cytokine Array A (20 T) Biotinylated Antibody Cocktail", "number":"AB197465-CMP01" }, { "size":"2 x 30 mL", "name":"20X Wash Buffer II", "number":"AB197465-CMP07" }, { "size":"2 x 1 Unit", "name":"Slide Washer/Dryer (30 mL Centrifuge Tube)", "number":"AB197465-CMP05" }, { "size":"2 x 1 Vial", "name":"Mouse Cytokine Array A (20 T) Lyophilized Cytokine Standard Mix", "number":"AB197465-CMP03" }, { "size":"4 x 1 Slide", "name":"Mouse Cytokine Array A (20 T) Glass Slide", "number":"AB197465-CMP02" }, { "size":"6 x 30 mL", "name":"20X Wash Buffer I", "number":"AB197465-CMP06" }, { "size":"4 x 5 µL", "name":"Cy3 equivalent dye-conjugated Streptavidin", "number":"AB197465-CMP08" }, { "size":"2 x 15 mL", "name":"Sample Diluent", "number":"AB197465-CMP04" } ] }, "8Test": { "sellingSize": "8 Test", "publicAssetCode":"ab197465-8Test", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Adhesive Film", "number":"AB197465-CMP09" }, { "size":"1 x 25 µL", "name":"Mouse Cytokine Array A (20 T) Biotinylated Antibody Cocktail", "number":"AB197465-CMP01" }, { "size":"1 x 1 Unit", "name":"Slide Washer/Dryer (30 mL Centrifuge Tube)", "number":"AB197465-CMP05" }, { "size":"1 x 15 mL", "name":"Sample Diluent", "number":"AB197465-CMP04" }, { "size":"1 x 5 µL", "name":"Cy3 equivalent dye-conjugated Streptavidin", "number":"AB197465-CMP08" }, { "size":"1 x 30 mL", "name":"20X Wash Buffer II", "number":"AB197465-CMP07" }, { "size":"2 x 30 mL", "name":"20X Wash Buffer I", "number":"AB197465-CMP06" }, { "size":"1 x 1 Slide", "name":"Mouse Cytokine Array A (20 T) Glass Slide", "number":"AB197465-CMP02" }, { "size":"1 x 1 Vial", "name":"Mouse Cytokine Array A (20 T) Lyophilized Cytokine Standard Mix", "number":"AB197465-CMP03" } ] }, "22Test": { "sellingSize": "22 Test", "publicAssetCode":"ab197465-22Test", "assetComponentDetails": [ { "size":"2 x 1 Unit", "name":"Adhesive Film", "number":"AB197465-CMP09", "productcode":"" }, { "size":"2 x 5 µL", "name":"Cy3 equivalent dye-conjugated Streptavidin", "number":"AB197465-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"Sample Diluent", "number":"AB197465-CMP04", "productcode":"" }, { "size":"1 x 30 mL", "name":"20X Wash Buffer II", "number":"AB197465-CMP07", "productcode":"" }, { "size":"3 x 30 mL", "name":"20X Wash Buffer I", "number":"AB197465-CMP06", "productcode":"" }, { "size":"2 x 1 Slide", "name":"Mouse Cytokine Array A (20 T) Glass Slide", "number":"AB197465-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Mouse Cytokine Array A (20 T) Lyophilized Cytokine Standard Mix", "number":"AB197465-CMP03", "productcode":"" }, { "size":"2 x 25 µL", "name":"Mouse Cytokine Array A (20 T) Biotinylated Antibody Cocktail", "number":"AB197465-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Slide Washer/Dryer (30 mL Centrifuge Tube)", "number":"AB197465-CMP05", "productcode":"" } ] } } }

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Product protocols

Target data

May participate in the recruitment of inflammatory cells by injured or infected tissue. GCP-2(1-78) and, more potent, GCP-2(9-78) attract neutrophils and are involved in neutrophil activation.
See full target information Cxcl5

Additional targets

Csf2rb,Il10,Csf1,Cxcl1,Ifng,Il12a,Il13,IL1A_MOUSE,Il1b,Il3,Il4,Il5,Il17a,Il6,Il9,Vegfa,Tnf,Il2,Cxcl9

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:10462 PubMed39668192

2024

Copper chelation redirects neutrophil function to enhance anti-GD2 antibody therapy in neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jourdin R C Rouaen,Antonietta Salerno,Tyler Shai-Hee,Jayne E Murray,Giulia Castrogiovanni,Charlotte McHenry,Toni Rose Jue,Vu Pham,Jessica Lilian Bell,Ensieh Poursani,Emanuele Valli,Riccardo Cazzoli,Naomi Damstra,Delia J Nelson,Kofi L P Stevens,Jonathan Chee,Iveta Slapetova,Maria Kasherman,Renee Whan,Francis Lin,Blake J Cochran,Nicodemus Tedla,Feyza Colakoglu Veli,Aysen Yuksel,Chelsea Mayoh,Federica Saletta,Daniele Mercatelli,Tatyana Chtanova,Arutha Kulasinghe,Daniel Catchpoole,Giuseppe Cirillo,Maté Biro,Holger N Lode,Fabio Luciani,Michelle Haber,Juliet C Gray,Toby N Trahair,Orazio Vittorio

Brain sciences 12: PubMed35884700

2022

The Dynamics of Tumor-Infiltrating Myeloid Cell Activation and the Cytokine Expression Profile in a Glioma Resection Site during the Post-Surgical Period in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jescelica Ortiz-Rivera,Alejandro Albors,Yuriy Kucheryavykh,Jeffrey K Harrison,Lilia Kucheryavykh

Molecular cancer therapeutics 19:2598-2611 PubMed32999046

2020

Anti-S100A4 Antibody Therapy Is Efficient in Treating Aggressive Prostate Cancer and Reversing Immunosuppression: Serum and Biopsy as a Clinical Predictor.

Applications

Unspecified application

Species

Unspecified reactive species

Arsheed A Ganaie,Adrian P Mansini,Tabish Hussain,Arpit Rao,Hifzur R Siddique,Ashraf Shabaneh,Marina G Ferrari,Paari Murugan,Jörg Klingelhöfer,Jinhua Wang,Noona Ambartsumian,Christopher A Warlick,Badrinath R Konety,Mohammad Saleem

Cancer research 80:4129-4144 PubMed32816860

2020

Intratumoral Copper Modulates PD-L1 Expression and Influences Tumor Immune Evasion.

Applications

Unspecified application

Species

Unspecified reactive species

Florida Voli,Emanuele Valli,Luigi Lerra,Kathleen Kimpton,Federica Saletta,Federico M Giorgi,Daniele Mercatelli,Jourdin R C Rouaen,Sylvie Shen,Jayne E Murray,Aria Ahmed-Cox,Giuseppe Cirillo,Chelsea Mayoh,Paul A Beavis,Michelle Haber,Joseph A Trapani,Maria Kavallaris,Orazio Vittorio

Glia 68:574-588 PubMed31652020

2019

Inhibition of inflammatory cells delays retinal degeneration in experimental retinal vein occlusion in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Joël Jovanovic,Xuan Liu,Despina Kokona,Martin S Zinkernagel,Andreas Ebneter
View all publications
websiteProtocolBooklet
en
websiteProtocolBooklet
en

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com