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Abcam Mouse Cytokine Antibody Array - Membrane (ab169820) detects 97 Mouse Cytokines.

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Multiplex Protein Detection - Mouse Cytokine Antibody Array - Membrane (97 targets) (AB169820), expandable thumbnail

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Key facts

Assay type
Semi-quantitative
Reactive species
Mouse
Target
Areg
(See target data below)

Target data

Function

Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.

Additional Targets

Ccl21b, Vegfd, Il21, Pro-epidermal growth factor, Slpi, Tnfrsf11b, Ccl3, Lgals1, Spp1, Il10, Il15, Sell, Cxcl15, Gzmd, Hepatocyte growth factor, Cxcl16, Ccl9, Il17a, Lepr, Madcam1, Flt1, Il2ra, Tnf, Il2, Tnfrsf19, Dickkopf-related protein 1, Mmp9, Epgn, F11r, Igfbp6, Growth arrest-specific protein 6, Tnfrsf13b, Tnfrsf18, Ccl2, Ctla4, Mfge8, Tnfsf11, Ccl17, Vegfd, Il21, Pro-epidermal growth factor, Slpi, Tnfrsf11b, Ccl3, Lgals1, Spp1, Il10, Il15, Sell, Cxcl15, Gzmd, Hepatocyte growth factor, Cxcl16, Ccl9, Il17a, Lepr, Madcam1, Flt1, Il2ra, Tnf, Il2, Tnfrsf19, Dickkopf-related protein 1, Mmp9, Epgn, F11r, Igfbp6, Growth arrest-specific protein 6, Tnfrsf13b, Tnfrsf18, Ccl2, Ctla4, Mfge8, Tnfsf11, Ccl17, Axl, Ccl11, Ccl19, Ccl20, Ccl22, Ccl24, Ccl5, Cd27, Cd36, CD70, Cdh1, Csf3, Ctf1, Cx3cl1, Cxcl1, Cxcl11, Cxcl13, Cxcl2, Cxcl9, Dcn, Faslg, Fcgr2, Flt3lg, Flt4, Ifng, Igf2, Igfbp5, Interleukin-11, Il12a, Il12b, Il13, Il17f, Il17rb, IL1A_MOUSE, Il1b, Il1rn, Il20, IL-25, Il3, Il4, Il5, Il6, Il9, Kitlg, Lep, Mmp2, Prl, Sele, Spint1, Thpo, Tnfrsf8, Tnfsf12, Tnfsf18, Tnfsf8, Vcam1, Vegfa

Alternative names

Sdgf, Areg, Amphiregulin, AR, Schwannoma-derived growth factor, SDGF

What's included?

1 x 2 Membranes
Components
1,000X HRP-Conjugated Streptavidin
1 x 50 µL
1X Blocking Buffer
1 x 25 mL
20X Wash Buffer I
1 x 10 mL
20X Wash Buffer II
1 x 10 mL
2X Cell Lysis Buffer
1 x 10 mL
4-Well Plastic Tray
1 x 1 Unit
4000X Biotin-Conjugated Anti Cytokines
2 x 1 Vial
Cytokine Antibody Array Membranes
1 x 2 Unit
Detection Buffer C
1 x 1.5 mL
Detection Buffer D
1 x 1.5 mL
Plastic sheets
1 x 1 Unit

Recommended products

Abcam Mouse Cytokine Antibody Array - Membrane (ab169820) detects 97 Mouse Cytokines.

Key facts

Assay type
Semi-quantitative
Reactive species
Mouse
Target
Areg
Applications
Multiplex Protein Detection
Sample types
Saliva, Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture media, Cell culture supernatant, Milk, Serum, Other biological fluids, Whole Blood, Cell Lysate

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

Abcam Mouse Cytokine Antibody Array - Membrane (ab169820) detects 97 Mouse Cytokines. Suitable for all liquid sample types.

Targets: 6Ckine, ALK-1, Amphiregulin, Axl, BLC, Cardiotrophin-1, CD27, CD27 Ligand, CD30, CD30 Ligand, CD36/SR-B3, CTLA-4, CXCL16, Decorin, Dkk-1, E-Cadherin, EGF, Eotaxin-1, Eotaxin-2, Epigen, E-Selectin, Fas Ligand, Fc gamma RIIB, Flt-3 Ligand, Fractalkine, Galectin-1, Gas 6, G-CSF, GITR, GITR Ligand, Granzyme B, HAI-1, HGF, IFN-gamma, IGFBP-5, IGFBP-6, IGF-2, IL-1 alpha, IL-1 beta, IL-1 ra, IL-2, IL-2 R alpha, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-11, IL-12 p40, IL-12 p70, IL-13, IL-15, IL-17, IL-17B R, IL-17E, IL-17F, IL-20, IL-21, I-TAC, JAM-A, KC/CXCL1, Leptin/OB, Leptin R, L-Selectin, Lungkine, MadCAM-1, MCP-1, MDC, MFG-E8, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 alpha, MIP-3 beta, MMP-2, Osteopontin, Osteoprotegerin, Prolactin, Pro-MMP-9, RANTES, SCF, sTNFRI, sTNFRII, TACI, TARC, TNF alpha, Thrombopoietin, TRANCE, TROY, TWEAK R, VCAM-1, VEGF-A, VEGFR1, VEGFR3, VEGF-D

Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.


**If you are interested in this cytokine array, a table listing all of our mouse membrane antibody cytokine arrays and other arrays and the analytes they measure is available .**


Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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1 product image

  • Multiplex Protein Detection - Mouse Cytokine Antibody Array - Membrane (97 targets) (ab169820), expandable thumbnail

    Multiplex Protein Detection - Mouse Cytokine Antibody Array - Membrane (97 targets) (ab169820)

    Typical results obtained with Abcam Membrane Antibody Arrays. The membrane was probed with conditioned media from two different cell lines. Membranes were exposed to film at RT for 1 min.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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