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ab133999 is for simultaneous detection of 40 Mouse inflammatory factors.

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Assay type

Semi-quantitative

Reactive species

Mouse

Target

Ccl11

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Images

Target data

What's included?

1 x 8 Membranes
Components
1,000X HRP-Conjugated Streptavidin
1 x 50 µL
1X Blocking Buffer
2 x 25 mL
20X Wash Buffer I
1 x 20 mL
20X Wash Buffer II
1 x 20 mL
2X Cell Lysis Buffer
1 x 16 mL
8-Well Incubation Tray (with Lid)
1 x 1 Unit
Biotin-Conjugated Anti-Cytokines
4 x 1 Vial
Detection Buffer C
1 x 2.5 mL
Detection Buffer D
1 x 2.5 mL
Inflammation Antibody Array Membranes
8 x 1 Unit

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ab133999 is for simultaneous detection of 40 Mouse inflammatory factors.

Key facts

Assay type

Semi-quantitative

Reactive species

Mouse

Target

Ccl11

Applications

Multiplex Protein Detection

Sample types

Saliva, Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture media, Cell culture supernatant, Milk, Serum, Other biological fluids, Whole Blood, Cell Lysate

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

ab133999 is for simultaneous detection of 40 Mouse inflammatory factors. Suitable for all sample types.

Targets: BLC, CD30L, Eotaxin, Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CSF, IFN-gamma, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, I-TAC, KC/CXCL1, Leptin/OB, LIX, Lymphotactin, MCP-1, MCSF, MIG, MIP-1 alpha, MIP-1 gamma, RANTES, SDF-1, TCA-3, TECK, TIMP-1, TIMP-2, TNF-alpha, sTNF RI, sTNF-RII

Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.

**A table listing all of our mouse membrane antibody cytokine arrays and other arrays and the analytes they measure is available .**

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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