JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB133999

Mouse Inflammation Antibody Array - Membrane (40 Targets)

Be the first to review this product! Submit a review

|

(33 Publications)

Mouse Inflammation Antibody Array - Membrane (40 Targets) ab133999 is a membrane based multiplex immunoassay for the simultaneous detection of 40 mouse inflammation factors in all sample types.

- More data, less sample: screen multiple inflammatory markers with the same sample
- Global view of inflammatory markers
- Chemiluminescent detection - no specific equipment required
- Highly sensitive (pg/mL)

View Alternative Names

Scya11, Ccl11, Eotaxin, C-C motif chemokine 11, Eosinophil chemotactic protein, Small-inducible cytokine A11

1 Images
Multiplex Protein Detection - Mouse Inflammation Antibody Array - Membrane (40 Targets) (AB133999)
  • Multiplex Protein Detection

Unknown

Multiplex Protein Detection - Mouse Inflammation Antibody Array - Membrane (40 Targets) (AB133999)

Typical results obtained with Abcam Membrane Antibody Arrays.
Membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at RT for 1 min.

Key facts

Assay type

Semi-quantitative

Reacts with

Mouse

Target

Ccl11

target

Applications

Multiplex Protein Detection

applications

Sample types

Saliva, Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture media, Cell culture supernatant, Milk, Serum, Other biological fluids, Whole Blood, Cell Lysate

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Multiplex Protein Detection": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Product details

ab133999 is for simultaneous detection of 40 Mouse inflammatory factors. Suitable for all sample types.

Targets: BLC, CD30L, Eotaxin, Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CSF, IFN-gamma, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, I-TAC, KC/CXCL1, Leptin/OB, LIX, Lymphotactin, MCP-1, MCSF, MIG, MIP-1 alpha, MIP-1 gamma, RANTES, SDF-1, TCA-3, TECK, TIMP-1, TIMP-2, TNF-alpha, sTNF RI, sTNF-RII

Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.

A table listing all of our mouse membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

What's included?

{ "values": { "1x8Membranes": { "sellingSize": "1 x 8 Membranes", "publicAssetCode":"ab133999-1x8Membranes", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plastic sheets", "number":"AB133999-CMP11" }, { "size":"2 x 4 Unit", "name":"Inflammation Antibody Array Membranes", "number":"AB133999-CMP10" }, { "size":"1 x 2.5 mL", "name":"Detection Buffer C", "number":"AB133999-CMP04" }, { "size":"1 x 2.5 mL", "name":"Detection Buffer D", "number":"AB133999-CMP05" }, { "size":"1 x 50 µL", "name":"1,000X HRP-Conjugated Streptavidin", "number":"AB133999-CMP03" }, { "size":"4 x 1 Vial", "name":"Biotin-Conjugated Anti-Cytokines", "number":"AB133999-CMP02" }, { "size":"1 x 16 mL", "name":"2X Cell Lysis Buffer", "number":"AB133999-CMP08" }, { "size":"1 x 20 mL", "name":"20X Wash Buffer II", "number":"AB133999-CMP07" }, { "size":"1 x 20 mL", "name":"20X Wash Buffer I", "number":"AB133999-CMP06" }, { "size":"2 x 25 mL", "name":"1X Blocking Buffer", "number":"AB133999-CMP01" }, { "size":"1 x 1 Unit", "name":"8-Well Incubation Tray (with Lid)", "number":"AB133999-CMP09" } ] }, "1x4Membranes": { "sellingSize": "1 x 4 Membranes", "publicAssetCode":"ab133999-1x4Membranes", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plastic sheets", "number":"AB133999-CMP11", "productcode":"" }, { "size":"1 x 4 Unit", "name":"Inflammation Antibody Array Membranes", "number":"AB133999-CMP10", "productcode":"" }, { "size":"1 x 1.5 mL", "name":"Detection Buffer D", "number":"AB133999-CMP05", "productcode":"" }, { "size":"1 x 50 µL", "name":"1,000X HRP-Conjugated Streptavidin", "number":"AB133999-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"8-Well Incubation Tray (with Lid)", "number":"AB133999-CMP09", "productcode":"" }, { "size":"1 x 1.5 mL", "name":"Detection Buffer C", "number":"AB133999-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"20X Wash Buffer II", "number":"AB133999-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotin-Conjugated Anti-Cytokines", "number":"AB133999-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"20X Wash Buffer I", "number":"AB133999-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"1X Blocking Buffer", "number":"AB133999-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"2X Cell Lysis Buffer", "number":"AB133999-CMP08", "productcode":"" } ] } } }

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Product protocols

Target data

In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils (a prominent feature of allergic inflammatory reactions), but not lymphocytes, macrophages or neutrophils (PubMed : 7568052, PubMed : 8574847). Binds to CCR3 (By similarity).
See full target information Ccl11

Additional targets

Csf2rb,Tnfrsf1b,Ccl3,Ccl1,Il10,Ccl9,Il17a,Tnf,Timp2,Il2,Ccl2,Tnfrsf1b,Ccl3,Ccl1,Il10,Ccl9,Il17a,Tnf,Timp2,Il2,Ccl2,Ccl24,Ccl25,Ccl5,Csf1,Csf3,Cx3cl1,Cxcl1,Cxcl11,Cxcl12,Cxcl13,Cxcl5,Cxcl9,Faslg,Ifng,Il12a,Il12b,Il13,IL1A_MOUSE,Il1b,Il3,Il4,Il6,Il9,Lep,Timp1,Tnfrsf1a,Tnfsf8,Xcl1

Publications (33)

Recent publications for all applications. Explore the full list and refine your search

Current issues in molecular biology 47: PubMed39996816

2025

The Immune-Enhancing Effects of a L-30 Extract Through the NF-κB and MAPK Pathways in RAW264.7.

Applications

Unspecified application

Species

Unspecified reactive species

Soyeon Kim,Inwook Kim,Sangkyu Park,Jeongmin Seo

Journal of translational medicine 23:207 PubMed39979938

2025

Intra and inter-organ communication through extracellular vesicles in obesity: functional role of obesesomes and steatosomes.

Applications

Unspecified application

Species

Unspecified reactive species

N Lago-Baameiro,T Camino,A Vazquez-Durán,A Sueiro,I Couto,F Santos,J Baltar,J M Falcón-Pérez,M Pardo

PloS one 20:e0318204 PubMed39883721

2025

Establishment of subcutaneous transplantation platform for delivering induced pluripotent stem cell-derived insulin-producing cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Thuan Tran,Watchareewan Rodprasert,Irma Padeta,Saranyou Oontawee,Steven Dwi Purbantoro,Anatcha Thongsit,Parkpoom Siriarchavatana,Sayamon Srisuwatanasagul,Hiroshi Egusa,Thanaphum Osathanon,Chenphop Sawangmake

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2309459 PubMed39049738

2024

PROTAC-Mediated HDAC7 Protein Degradation Unveils Its Deacetylase-Independent Proinflammatory Function in Macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Kailibinuer Kadier,Tian Niu,Baoli Ding,Boya Chen,Xuxin Qi,Danni Chen,Xirui Cheng,Yizheng Fang,Jiahao Zhou,Wenyi Zhao,Zeqi Liu,Yi Yuan,Zhan Zhou,Xiaowu Dong,Bo Yang,Qiaojun He,Ji Cao,Li Jiang,Cheng-Liang Zhu

International journal of molecular sciences 25: PubMed38673795

2024

Tumor-Extrinsic Axl Expression Shapes an Inflammatory Microenvironment Independent of Tumor Cell Promoting Axl Signaling in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kristina Breitenecker,Denise Heiden,Tobias Demmer,Gerhard Weber,Ana-Maria Primorac,Viola Hedrich,Gregor Ortmayr,Thomas Gruenberger,Patrick Starlinger,Dietmar Herndler-Brandstetter,Iros Barozzi,Wolfgang Mikulits

Journal of nanobiotechnology 21:371 PubMed37821897

2023

Nanobody-based pannexin1 channel inhibitors reduce inflammation in acute liver injury.

Applications

Unspecified application

Species

Unspecified reactive species

Raf Van Campenhout,Timo W M De Groof,Prashant Kadam,Brenda R Kwak,Serge Muyldermans,Nick Devoogdt,Mathieu Vinken

Nature communications 14:5660 PubMed37704610

2023

De novo design of highly selective miniprotein inhibitors of integrins αvβ6 and αvβ8.

Applications

Unspecified application

Species

Unspecified reactive species

Anindya Roy,Lei Shi,Ashley Chang,Xianchi Dong,Andres Fernandez,John C Kraft,Jing Li,Viet Q Le,Rebecca Viazzo Winegar,Gerald Maxwell Cherf,Dean Slocum,P Daniel Poulson,Garrett E Casper,Mary L Vallecillo-Zúniga,Jonard Corpuz Valdoz,Marcos C Miranda,Hua Bai,Yakov Kipnis,Audrey Olshefsky,Tanu Priya,Lauren Carter,Rashmi Ravichandran,Cameron M Chow,Max R Johnson,Suna Cheng,McKaela Smith,Catherine Overed-Sayer,Donna K Finch,David Lowe,Asim K Bera,Gustavo Matute-Bello,Timothy P Birkland,Frank DiMaio,Ganesh Raghu,Jennifer R Cochran,Lance J Stewart,Melody G Campbell,Pam M Van Ry,Timothy Springer,David Baker

BMB reports 56:439-444 PubMed37357536

2023

Regorafenib prevents the development of emphysema in a murine elastase model.

Applications

Unspecified application

Species

Unspecified reactive species

Kwangseok Oh,Gun-Wu Lee,Han-Byeol Kim,Jin-Hee Park,Eun-Young Shin,Eung-Gook Kim

Stem cell reports 18:969-984 PubMed37044069

2023

Lovastatin promotes the self-renewal of murine and primate spermatogonial stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chaohui Li,Zhaokai Yao,Linzi Ma,Xiuling Song,Wen Wang,Cong Wan,Shaofang Ren,Dingyao Chen,Yi Zheng,Yong-Tong Zhu,Gang Chang,Shihao Wu,Kai Miao,Fang Luo,Xiao-Yang Zhao

International journal of biological sciences 19:1123-1145 PubMed36923940

2023

Low-intensity pulsed ultrasound promotes skeletal muscle regeneration via modulating the inflammatory immune microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Haocheng Qin,Zhiwen Luo,Yaying Sun,Zhong He,Beijie Qi,Yisheng Chen,Junlong Wang,Ce Li,Weiwei Lin,Zhihua Han,Yulian Zhu
View all publications
websiteProtocolBooklet
en

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com