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ab133999 is for simultaneous detection of 40 Mouse inflammatory factors.

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Multiplex Protein Detection - Mouse Inflammation Antibody Array - Membrane (40 Targets) (AB133999), expandable thumbnail

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Key facts

Assay type
Semi-quantitative
Reactive species
Mouse
Target
Ccl11
(See target data below)

Target data

Function

In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils (a prominent feature of allergic inflammatory reactions), but not lymphocytes, macrophages or neutrophils (PubMed:7568052, PubMed:8574847). Binds to CCR3 (By similarity).

Additional Targets

Csf2rb, Tnfrsf1b, Ccl3, Ccl1, Il10, Ccl9, Il17a, Tnf, Timp2, Il2, Ccl2, Tnfrsf1b, Ccl3, Ccl1, Il10, Ccl9, Il17a, Tnf, Timp2, Il2, Ccl2, Ccl24, Ccl25, Ccl5, Csf1, Csf3, Cx3cl1, Cxcl1, Cxcl11, Cxcl12, Cxcl13, Cxcl5, Cxcl9, Faslg, Ifng, Il12a, Il12b, Il13, IL1A_MOUSE, Il1b, Il3, Il4, Il6, Il9, Lep, Timp1, Tnfrsf1a, Tnfsf8, Xcl1

Alternative names

Scya11, Ccl11, Eotaxin, C-C motif chemokine 11, Eosinophil chemotactic protein, Small-inducible cytokine A11

What's included?

1 x 8 Membranes
Components
1,000X HRP-Conjugated Streptavidin
1 x 50 µL
1X Blocking Buffer
2 x 25 mL
20X Wash Buffer I
1 x 20 mL
20X Wash Buffer II
1 x 20 mL
2X Cell Lysis Buffer
1 x 16 mL
8-Well Incubation Tray (with Lid)
1 x 1 Unit
Biotin-Conjugated Anti-Cytokines
4 x 1 Vial
Detection Buffer C
1 x 2.5 mL
Detection Buffer D
1 x 2.5 mL
Inflammation Antibody Array Membranes
2 x 4 Unit
Plastic sheets
1 x 1 Unit

Recommended products

ab133999 is for simultaneous detection of 40 Mouse inflammatory factors.

Key facts

Assay type
Semi-quantitative
Reactive species
Mouse
Target
Ccl11
Applications
Multiplex Protein Detection
Sample types
Saliva, Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture media, Cell culture supernatant, Milk, Serum, Other biological fluids, Whole Blood, Cell Lysate

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

ab133999 is for simultaneous detection of 40 Mouse inflammatory factors. Suitable for all sample types.

Targets: BLC, CD30L, Eotaxin, Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CSF, IFN-gamma, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, I-TAC, KC/CXCL1, Leptin/OB, LIX, Lymphotactin, MCP-1, MCSF, MIG, MIP-1 alpha, MIP-1 gamma, RANTES, SDF-1, TCA-3, TECK, TIMP-1, TIMP-2, TNF-alpha, sTNF RI, sTNF-RII

Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.


**A table listing all of our mouse membrane antibody cytokine arrays and other arrays and the analytes they measure is available .**


Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Multiplex Protein Detection - Mouse Inflammation Antibody Array - Membrane (40 Targets) (ab133999), expandable thumbnail

    Multiplex Protein Detection - Mouse Inflammation Antibody Array - Membrane (40 Targets) (ab133999)

    Typical results obtained with Abcam Membrane Antibody Arrays.

    Membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at RT for 1 min.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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